CBS
Refresher Lab Safety
Training
Please sign the session roster sheets. This training
session will be recorded in Peoplesoft. (the sheets
are available as you enter. Sign at your name.)
If you are not on the list, write your name LEGIBLY
and please include your Employee ID number.
David Okita
Biochemistry, Molecular Biology & Biophysics
6-155 Jackson Hall
okita001@umn.edu
(612) 624-7107
This sessions covers Lab
Safety, Chemical Safety and
Chemical Waste Management
Refresher Training Only!
General Lab Safety Training
Required for All
All University of Minnesota faculty, staff, and students
that work in or supervise individuals working in
laboratories are required by law to receive training
regarding safety issues and practices in their work place.
Training is mandated by both the federal Laboratory
Safety Standard and the Minnesota Employee Right to
Know Act and is required upon hire, at refresher intervals
(at least annually), and whenever a new procedure is
introduced.
Environmental Health and Safety (DEHS) provides basic
safety training for new faculty, staff, and students. Three
web-based tutorials that are mandatory for all lab
workers are available at
http://www.dehs.umn.edu/training_newlabsafety.htm
Lab Specific Training
Encouraged for All
Each Principal Investigator and/or laboratory supervisor is
responsible for ensuring that employees are provided
with training addressing specific hazards (chemical,
physical, and biological) in their laboratory or work area.
Training must address the specific hazards of the
procedure and personal protective equipment available to
reduce the potential risk of exposure to the particular
hazard. Training must be provided at the time of an
employee's initial work assignment and prior to
assignments involving any new potentially hazardous
situations. Refresher training must be provided annually.
Specific Material Use Training
In addition to general Lab Safety and Chemical Waste
Management training, work with the following materials
requires additional training:
• Bloodborne and Other Pathogens if work involves
human blood, human body fluids, human cells (including cell
lines), unfixed human tissue, and/or infectious agents
(viruses, bacteria, fungi, rickettsia, prions), Online tutorial.
Must be renewed or reviewed every year.
http://www.dehs.umn.edu/bio_pracprin_blood_bpt.htm
• Hazardous Shipping for workers who package and ship
hazardous materials, including infectious substances or
diagnostic specimens.
In Person training sessions. Once every 2 years.
http://www.dehs.umn.edu/training_shiphazmat.htm.
• Controlled Substance users must complete training
through Regulatory Affairs. One time on person training
session.
http://www.research.umn.edu/regaffairs/ControlledSub/inde
x.html
• Animal Users must complete training through IACUC,
http://www.research.umn.edu/iacuc/training/ .
• Radiation training is required by the Nuclear Regulatory
Commission (NRC) for persons who work in or frequent a
radioactive materials area. For further information call
Environmental Health and Safety at 612-626-6002.
Updates
•Compliance Inspections
•Injury Reports
Inspections by the County,
AAALAC, EPA, DEHS and
your departmental RSO have
indicated some improvements
but we still have some
problems.
Good News!
Signs of Improvement
•Less food in the labs
•Better Labeling and Segregation
of Hazardous Waste
•The EPA really liked the Posters
and and saw lots of Yellow labels
•Better Lab Attire
Proper Lab Attire
Chapter 3, section 2 of the University of Minnesota Lab Safety Plan
Skin must be protected from hazardous liquids, gases and vapors, proper basic
attire is essential in the laboratory. Long hair should be pulled back and secured
and loose clothing (sleeves, bulky pants or skirts) avoided to prevent accidental
bare feet, sandals and
open-toed or perforated shoes are not permitted in any
laboratory. Short pants and short skirts are not permitted
unless covered by a lab coat. Long pants should be worn to cover
contact with chemicals or open flames. However,
skin that could be exposed during a spill.
Wear shoes that cover your skin
Good
Bad
Areas That Need Improvement
Chemical Storage
•Top Issue: Incompatible
chemicals stored together
(e.g. acids and bases or
organics and in-organics
stored together)
•Chemicals and Solvents
without secondary
containment
•Chemical Labeling
Containers not labeled
•Sharps Containers
Overfilled or improper items
(e.g. pipettes)
Do Not Recap Needles
•Eyewashes need to be inspected and
documented weekly!
Injuries and Accidents!
Numerous lab accidents.
Explosions, Fires, Burns, Cuts, Spills and
Fainting!
A few injuries requiring Workman’s Compensation
were reported.
How can we avoid these?
•Review your SOPs and make sure to
address the specific hazards for each
procedure!
•Lab Audits
Reporting Injuries
Forms available at the U of M forms library
http://process.umn.edu/groups/ppd/documents/main/formho
me.cfm
Or
Contact your Department RSO
Autoclaving
Biological Waste
What is Autoclaved:
All disposable lab ware contaminated with potentially infectious
materials (blood, body fluids, human cell culture media, bacteria
culture media)
Except Pasteur pipettes, Pasteur pipettes should be disposed of in
red sharps container.
Culture plates
Culture media
Animal cages and bedding from infected animals
Autoclaving Biological Waste
Procedure:
Place materials in a clear autoclave bag (available from U stores). Do not use
the RED or ORANGE biohazard bags to autoclave waste since these bags
cannot be disposed of as regular trash.
No Biohazard symbol should be visible. The University pays a premium to
dispose biohazard waste.
Bag should be loosely packed and not more than 3/4 full. Do not seal bag shut. If
using a rubber band, place rubber band on loosely, do not twist opening should
be at least one inch in diameter.
Using an industrial strength permanent marker write in Large Clearly Identifiable
Markings.
Your PI Last Name, Room Number and the Date you prepared the waste for
autoclaving.
Autoclaving Procedure
Use autoclave indicator tape on outside of bag to show that waste has been
processed.
Place waste material on a large, metal, leak-proof tray.
Metal containers transfer heat more efficiently than plastic containers.
Container should be large enough and shallow enough to allow for ample
steam circulation.
If autoclaving more than one bag at a time, be sure there is ample room
between the bags so steam circulation is not impaired.
Autoclave at 121ｰC for 60 minutes.
After autoclaving is complete, tape bag shut or tighten rubber band and place
in regular trash receptacle.
Liquid Waste
To autoclave liquid waste, place liquid in beaker or flask,
not in autoclave bag.
Autoclaved liquid culture waste can be sewered unless
hazardous chemical waste is present. If hazardous
chemical waste is present follow procedures in the
Hazardous Chemical Waste Management Guidebook at
http://www.dehs.umn.edu/hazwaste_chemwaste_umn_cw
mgbk_sec2.htm.
Do not sewer melted agar as it will congeal and clog the
plumbing.
Autoclave Testing:
Autoclave indicator tape does not prove decontamination
effectiveness.
For highest confidence in decontamination effectiveness, each load
should be tested.
If autoclaving for less than 60 minutes, each load must be tested.
3M Comply (Thermalog) chemical integrator strips provide
immediate test results.
These can be purchased from U Stores - CX12607.
Attach the indicator strip to a stick or string and put in the center of
the load.Retrieve after autoclaving for confirmation that the entire
load has been exposed to the conditions necessary for
decontamination.If the indicator reads unsafe, autoclave again.
Let be careful out there!
•Remember to do your lab audits
•Check to be sure your protocols cover safety
hazards and mitigation plans.
• Contact your Department RSO
•Getting rusty? You might consider re-doing
the initial training online at
http://www.dehs.umn.edu/training_newlabsafety.htm
Laboratory Poster Р
Take One W ith You Today!
Remember
You must sign the session roster
sheets for this training to be recorded
in Peoplesoft (the sheets are
available as you enter. Sign at your
name.)
If you are not on the list, write your
name LEGIBLY at the end of the roster.
Please include ID number too.