Microbiology Lab Report 1 – Laboratory Safety and Making Media
Name:
Question 1: The following is a list of the microorganisms that we will be using
during the first half of this semester. Which of these are BSL-2?
Staphylococcus epidermidis
Micrococcus luteus
Bacillus megaterium
Bacillus subtilis
Escherichia coli
Corynebacterium xerosis
Mycobacterium smegmatis
Klebsiella pneumonia
Providencia stuartii
Enterobacter aerogenes
Kosurea rosea
Alcaligenes faecalis
Pseudomonas aeruginosa
Citrobacter freundii
Micrococcus luteus
Question 2: How do the safety requirements for BSL-2 differ from those for BSL-1?
Question 3: Where would you dispose of a paper towel that had been used to clean
up a microbial spill?
Question 4: Where would you dispose of a paper towel that had been used to wipe
down your lab bench at the end of the lab period?
Question 5: Where would you dispose of a broken test tube that was contaminated
by a microbial culture?
Question 6: Where would you dispose of a used petri plate?
Question 7: For each of the media-making techniques below, write the words “agar
plate” “agar slant” and/or “broth tube” to indicate which of the media types require
the use of that technique. Use any, all, or none of the media types, as appropriate.
Microwave the mixture before disbursing into containers for autoclaving:
Autoclave the entire mixture in one container and then disburse to smaller
containers for use:
Autoclave in test tubes:
Allow autoclaved mixture to cool in a slanted position:
Cover container loosely before autoclaving:
Store completed medium upside down:
Clean up entire prep area after making the medium: