NAME: ____________________________ GENETICS PACKET ***DO NOT LOSE ME!*** PERIOD: ____ COMPLETE DOMINANCE 1. Describe the genotypes given. a. DD Homozygous dominant d. ss _____________________ b. Dd Heterozygous e. Yy ______________________ c. dd ____________________ f. WW _____________________ 2. In humans, brown eyes (B) is dominant over blue eyes (b). What are the phenotypes of the following genotypes? a. BB _____________________ b. Bb _____________________ c. bb ______________________ ***ON A SEPARATE SHEET OF PAPER THAT WILL BE TURNED IN, DO THE FOLLOWING PROBLEMS: Title the page: COMPLETE DOMINANCE Each genetic cross must show the parent alleles, the full Punnett square, and complete genotypic and phenotypic ratios. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. What does “complete dominance” mean? A heterozygous tall pea plant is crossed with a homozygous short pea plant. T = Tall and t = short. A white mouse is mated with a black mouse. Both were from a pure strain (homozygous). B = black, b = white. Any time a homozygous dominant and homozygous recessive are crossed, the offspring can be referred to as the F1 generation. Cross two mice from the F1 generation of problem #3. Black eyes in mice is dominant over red. A black-eyed homozygous male mates with a black-eyed heterozygous female. Use B = black, b = red. A red-eyed male mates with a homozygous black-eyed female. Show the cross between the F1 generation of problem #6. Blue flowers is dominant over white. A plant with white flowers is crossed with a plant with blue flowers. The blue flowered plant had a parent whose flowers were white. Use B = blue, b = white. Two heterozygous blue-flowered plants are crossed. Two homozygous white-flowered plants are crossed. 1 Title the page: More Punnett square practice Use a Punnett Square, genotypic and phenotypic ratios to show the possible offspring from the crosses given and answer the questions. IN PEAS: R = round r=wrinkled T= tall t = short Y = yellow peas P = purple flowers y = green peas p = white flowers 1. Make a cross between a PURE YELLOW SEED parent and a PURE GREEN SEED parent. What is the probability an offspring will show the DOMINANT TRAIT (YELLOW SEEDS)? _________ What is the probability an offspring will show the RECESSIVE TRAIT (GREEN SEEDS)? ________ 2. Make a cross between a HOMOZYGOUS PURPLE FLOWERED plant and a HOMOZYGOUS WHITE FLOWERED plant. What is the probability an offspring will show the DOMINANT TRAIT (PURPLE FLOWERS)? ___________ What is the probability an offspring will show the RECESSIVE TRAIT (WHITE FLOWERS)? _____________ 3. SEE A PATTERN FOR HOMOZYGOUS MONOHYBRID CROSSES: If you cross plants that are PURE DOMINANT for a trait with plants that are PURE RECESSIVE for that trait, ________ % of the offspring will show the DOMINANT trait and the ___________________ trait will not show at all. USE THE PATTERN ABOVE TO MAKE PREDICTION ABOUT A CROSS 4. If you cross a HOMOZYGOUS ROUND SEED plant with a PURE WRINKLED SEED plant 100% will look __________________ 0% will look ____________________ The offspring will have a 100% probability of having the ______ genotype. MAKE SOME HETEROZYGOUS MONOHYBRID CROSSES 5. A black coat (B) is DOMINANT in guinea pigs. A brown coat (b) is RECESSIVE. What is the genotype of a HOMOZYGOUS BLACK guinea pig? = _______ What is the GENOTYPE of a HETEROZYGOUS BLACK guinea pig? =_________ What is the GENOTYPE of a brown guinea pig? =__________ 6. Make a cross between TWO HETEROZYGOUS BLACK guinea pigs. What is the probability that a baby will be black? _________ What is the probability that a baby will be brown? _________ What is the probability the baby will be a HYBRID? ________ What is the probability the baby will be HOMOZYGOUS DOMINANT? _________ What is the probability the baby will be HOMOZYGOUS RECESSIVE? ___________ 2 7. Purple fur (P) is DOMINANT in monsters. Yellow fur (p) is RECESSIVE. What is the genotype of a PURE PURPLE monster? = ________ What is the GENOTYPE of a HETEROZYGOUS purple monster? _________ What is the GENOTYPE of a YELLOW monster? = ________ 8. Make a cross between TWO HETEROZYGOUS PURPLE MONSTERS. What is the probability that a baby will be purple? __________ What is the probability that a baby will be yellow? __________ What is the probability the baby will be a heterozygous? __________ What is the probability the baby will be HOMOZYGOUS DOMINANT? _________ What is the probability the baby will be HOMOZYGOUS RECESSIVE? ___________ SEE A PATTERN FOR HETEROZYGOUS MONOHYBRID CROSSES: 9. If you cross 2 parent plants that are BOTH HETEROZYGOUS for a trait the offspring will show a ____ : ____ phenotypic ratio. ________ % of the offspring will show the DOMINANT trait and ________ % of the offspring will show the RECESSIVE trait. USE DOMINANT OR RECESSIVE TO FILL IN THE BLANKS BELOW: ¼ of the offspring will have two __________________ alleles, ½ will be hybrids with one _______________ and one _______________ allele, and ¼ will have two _________________ alleles. USE THE PATTERN ABOVE TO MAKE PREDICTION ABOUT A CROSS 10. If you cross two HETEROZYGOUS ROUND SEED plants 75% will look __________________ 25% will look __________________ The probability is: ¼ will have the genotype ______ ½ will have the genotype _____ ¼ will have the genotype _____ BACK CROSS / TEST CROSS 11. When an organism has the dominant phenotype, you cannot tell its genotype by looking at it. For example, if B = black and b = brown, a black guinea pig may be BB or Bb. How can we find out? Below, show the cross you would make with your B__ guinea pig that would give us the most information about the other allele. In other words, what can you cross the pig with to determine if it is BB or Bb? 3 INCOMPLETE DOMINANCE Incomplete dominance is a genetic phenomenon in which an individual displays a trait that is intermediate between the two parents. Another way to describe it is that there is a “blending” of the traits. In the problems we have done so far, a homozygous dominant individual has the same phenotype as a heterozygous individual. That is, a BB black mouse looks just like a Bb black mouse. There are some genes for which this is not true. For example, in Japanese four-o’clocks (a type of flower), plants with two red alleles (RR) have red flowers. Plants with two white (rr) alleles have white flowers. However, plants with one red allele and one white allele (Rr) are pink! Perform the following crosses in the same way you would for complete dominance. You may continue to use one capital and one lower case letter, or you may use two different letters. Just remember that a heterozygote now looks different (and will be recorded differently in the phenotype) from a homozygous dominant individual. ** ON A SEPARATE SHEET OF PAPER THAT WILL BE TURNED IN, DO THE FOLLOWING PROBLEMS: Title the page: INCOMPLETE DOMINANCE CROSSES Each genetic cross must show the parent alleles, the full punnett square, and complete genotypic and phenotypic ratios. 1. Predict the outcomes of the following crosses of Japanese four-o’clocks: a. a red plant and a white plant b. a white plant and a pink plant c. a red plant and a pink plant d. two pink plants 2. In some cats the gene for tail length shows incomplete dominance. Cats with long tails and those with no tails are homozygous for the respective alleles. Cats with one long tail allele and one no tail allele have short tails. First pick the letters you will use for each trait, then predict the outcomes of crosses between: a. a long tail cat and a cat with no tail b. a long tail cat and a short tail cat c. two short tail cats 3. In guinea pigs, brown fur (B) is dominant to white (b). The heterozygous condition results in creamcolored fur. Cross a brown guinea pig with a cream-colored guinea pig. Provide the genotypic and phenotypic ratios. 4 CODOMINANCE Codominance is a genetic phenomenon in which a heterozygote shows BOTH traits. For example, a black mouse crossed with a white mouse will look black and white. Crosses for these traits are done the same as crosses for complete or incomplete dominance. *Add these problems to your incomplete dominance page but label a new section “Codominance”. 1. In “pufferbelly pigs” spot color is inherited through codominance. Some pufferbelly pigs have pink skin with green spots (G), while others have pink skin with yellow spots (g). Some really special pufferbelly pigs have pink skin with green and yellow spots. List the three possible genotypes and phenotypes for pufferbelly pig spot color below (set up a key): Show the results (genotypes, phenotypes and their percentages) of a cross between two pufferbelly pigs heterozygous for the spot color gene. Once upon a time… 2. There were dragons that wanted to have baby dragons. The female had hot flame breath (HH) and the male had cool flame breath (hh). The warm breath gene is (Hh). List the three possible genotypes and phenotypes for dragon breath below (set up a key): Complete a Punnett square for the cross of the male and female in the original question. What are the genotypes and phenotypes that could result from this cross? 3. Scale color in dragons is controlled by a gene. The blue spot color gene is B and the yellow spot color gene is b. Some dragons have yellow and blue spots. List the three possible genotypes and phenotypes for dragon spot color below (set up a key): Complete a Punnett square for a cross between dragons – both with blue and yellow spots. What are the genotypes and phenotypes that could result from this cross? 5 Practice: Codominance and Incomplete Dominance 1. Practice setting up keys for the phenotypes listed in each set. Remember that the "medium" trait must always be heterozygous. a) Birds can be blue (B), white (b), or white with blue-tipped feathers. b) Flowers can be white (W), pink, or red (w). c) A Hoo can have curly hair (H), spiked hair (h), or a mix of both curly and spiked. d) A Sneech can be tall (T), medium, or short (t). e) A Bleexo can be spotted, black (B), or white (b). 2. Now, can you figure out in the above (1a-1e), which of the letters (a, b, c, d, and e) represent codominant traits and which are incomplete? Codominant are letters: _____________ Incompletely Dominant are letters:________________ 6 3. In Smileys, eye shape can be starred (E), circular (e), or a circle with a star. Write the genotypes for the pictured phenotypes on the lines below each Smiley. ________ ________ ________ 4. Show the cross between a star-eyed and a circle eyed. What are the phenotype ratios of the offspring? ____________ What are the genotype ratios? __________ 5. Show the cross between a circle-star eyed, and a circle eyed. How many of the offspring are circle-eyed? ____________ How many of the offspring are circle-star eyed? ____________ 6. Show the cross between two circle-star eyed. How many of the offspring are circle-eyed? ____________ How many of the offspring are circle-star eyed? ____________ How many are star eyed? ____________ 7 DIHYBRID CROSSES AND BEYOND For all of the crosses we’ve done so far, we’ve only considered one trait at a time. Is a pea plant tall or short? Does a pea plant have round seeds or wrinkled seeds? Well, what if we want to know the chances of two traits showing up in the offspring? We must consider a dihybrid cross for this. How To Solve a Dihybrid Cross 1. Read the problem and find the two separate traits. In Springfield, red hair (H) is dominant to blue hair (h) and having four fingers (F) on your hand is dominant to having five fingers (f). If Side Show Bob and Mrs. Van Houten get married and decide to have a little brother for Millhouse, what is the chance that the newborn will have red hair and five fingers if Side Show is heterozygous for red hair and heterozygous for four fingers and Mrs. Van Houten has blue hair and is homozygous for four fingers? 2. Find the two parents and write down their genotypes. Dad’s alleles = ______________________________ Mom’s alleles = ______________________________ 3. Read the question and see what you have to look for. Do they want you to find a ratio, percent, fraction, or do they want you to list all possibilities? 4. Once you have found all the information, you can start to set up the problem by finding the gametes for each parent by using FOIL. • Here is an example of how to use FOIL: • (a + b) * (c + d) • First = (a + b) * (c + d) = ac • Outside = (a + b) * (c + d) = ad • Inside = (a + b) * (c + d) = bc • Last = (a + b) * (c + d) = bd • So our pairs are: ac, ad, bc, bd • Here is another example a b c ac bc d ad bd Dad’s gametes = ________, ________,________,________ Mom’s gametes = ________,________,________,________ 8 5. Once you have the gametes, you have to line them up in a punnett square. Put the gametes of one parent across the top of the square and the other down the side of the square 6. Now you have to do the actual cross. Fill in the rest of the boxes! 7. Find the genotypic ratio. Genotypic ratio: 8. Find the phenotypic ratio. Phenotypic ratio: 9. Now, don’t forget to answer the question! 9 ***In the space below, do the following problems. Trait Body color Dominant Phenotype (B) purple Recessive Phenotype (b) red Tentacle suckers (A) present (a) absent Size of Lips (L) thin (l) thick Diet / Type of Teeth (D) pointed = carnivore (d) blunt = herbivore # of Tentacles (T) 8 (t) 4 1. A carnivorous male with 4 tentacles mates with an 8-tentacled female who also eats meat. The male is heterozygous for the diet trait. The female's tentacle genotype is hybrid but her diet genotype is pure. What is the chance this couple will produce a carnivorous baby with 8 tentacles? 2. A red male with thin lips mates with a thin-lipped purple female. The female is heterozygous for the body color trait. As for the lips, both aliens are heterozygotes. What is the chance that their baby will be red and have thick lips? 3. Rock, a thin-lipped male that lacks tentacle suckers, is having a baby with Amber, a thick-lipped female who also lacks tentacle suckers. Rock fell in love with Amber because of her thick lips. Rock's mother had thick lips and it is common for this alien species to select a mate with features similar to the maternal parent. What is the chance that Amber will give birth to a thin-lipped baby that has tentacle suckers? 10 Dihybrid Cross Poster Project Objective: This project involves creativity as well as genetics. You will choose any “organism” and two traits to cross. You will diagram the cross on a poster. In each square of the diagram, you will write the genotype of the offspring and draw the phenotype. Key: 1. Choose a thing to cross. It does not have to be a living thing, just something fun to cross and something you can draw without too much trouble. Example: Frogs I will cross: ___________________________________________ 2. Choose two traits you will examine. Example: color and tongue length I will examine: _________________________ and _______________________ 3. Define the alleles for your traits. You will need to take into account how many alleles there will be for each trait and what pattern of inheritance they will follow (complete, incomplete, or codominance). Use your notes to come up with creative, challenging allele possibilities. Example: Color (GG = green; Gg = green; gg = blue) Tongue length (LL = long; Ll = long; ll = short) My alleles will be: TRAIT: ________________________ Alleles: ______ = ______________ ______ = ______________ ______ = ______________ TRAIT: ________________________ Alleles: ______ =_______________ ______ = _______________ ______ = _______________ 4. Now, decide the phenotypes and genotypes of the parents. Remember, the more varied your parent genotype is, the more interesting phenotypes you will get in the offspring. More variety = better grade! Example: I will cross a green, long tongued male with a blue, long tongued female Phenotype of my parents: _________________________ x _______________ Example: I will cross GgLl x ggLL Genotype of my parents: _______________________ x __________________ 11 5. FOIL their genotypes to determine their possible gametes. Dad’s gametes: __________________________________ Mom’s gametes: _________________________________ 6. Complete the cross. 7. Determine the genotypic ratio of your cross: 8. Determine the phenotypic ratio of your cross: 9. Now you are ready to make your poster! Be sure to include: name/description of the “organism” (living, non-living, fictional) description/key of the TWO traits and the alleles/versions of each (you will receive more points for using co-dominance or incomplete dominance – but be careful, it’s harder!) parent’s genotypes and a PICTURE of their phenotypes (you will receive more points for using heterozygous genotypes) list of parents gametes (results of FOIL) dihybrid punnett square (each box should include the offspring’s genotype AND a picture of its phenotype) list of possible genotypes and phenotypes with fractions ** Pictures can be drawn or computer generated. ** Be neat. Include all required information. ** Remember – you determine what the traits, alleles, and parents will be. 12 Dihybrid Poster Scoring Rubric Your score: ________ / 3 points Both Traits are complete dominance +2 pts. One trait is Incomplete or Codominant +4 pts. Both traits are Incomplete or Codominant ________ / 2 points Parents genotype – homozygous +2 pts. some traits heterozygous +4 pts. all traits heterozygous ________ / 2 points Parents phenotypes ________ / 4 points Correct gametes/Allele combinations outside of punnett square ________ / 4 points Correct genotypes of possible offspring inside punnett square ________ / 4 points Correct phenotypes of possible offspring inside the punnett square ________ / 4 points Correct genotypic ratio of offspring ________ / 4 points Correct phenotypic ratio of offspring ________ / 3 points Appearance of poster – correct paper, colorful, clear drawings of traits, neat ________ / 30 points total score 13 REVIEW 4 types of basic genetics problems Complete Dominance Incomplete Dominance Co-dominance Dihybrid Cross Characteristics: Characteristics: Characteristics: Characteristics: Example: Example: Example: Example: 14