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Genome Sequence of Bacillus sp. Strain FJAT-14515
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Liu guohonga, Liu Boa, Tang Weiqib, Che Jianmeia, Lin Yingzhia, Zhu Yujinga, Su
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Mingxinga,Tang Jianyanga
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Agricultural Bio-resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China a.
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Fujian agicultural and foresty university, Fuzhou, Fujian 350003, Chinab.
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E-mail: Liu Guohong liuguohong624@163.com, Liu Bo fzliubo@163.com, Tang Weiqi
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tweiqi@163.com, Che Jianmei chejm2002@163.com, Lin Yingzhi lynnet@163.com, Zhu Yujing
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zyjingfz@163.com, Su Mingxing starsu717@163.com, Tang Jianyang tjy836@163.com
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ABSTRACT
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We report here the draft genome sequence of Bacillus sp. strain FJAT-14515. The
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genome is 5.44 M in length, and it covers 5,263 genes with an average length of 791
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bp and a G+C value of 37%.06, 67 tRNAs, 31sRNAs and 5 rRNA loci.
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GENOME ANNOUNCEMENT
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Bacillus sp. strain FJAT-14515 (16S rRNA GenBank accession number
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JX262264), a mesophiles and endospore-forming bacterium, was isolated from the
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soil sample collected from Taiwan. It grows optimally at a 0% NaCl (range, 0 to 5%)
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concentration in Nutrient Agar (NA) at 30 °C (10-40 °C) and pH 7.0 (6-9). The 16S
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rRNA similarities between FJAT-14515 and closest strains B. muralis DSM 16288T
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and B. simplex DSM 30646T were less than 98% through EzTaxon-e database
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(http://eztaxon-e.ezbiocloud.net/)(1). Devereux et al. (2) and Fry et al. (3) have proposed
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that a similarity of less than 98% in a 16SrRNA sequence should be considered
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evidence for separate species. So, the genome of Bacillus sp. FJAT-14515 was
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sequenced with a view to determining whether it was a novel species of the genus
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Bacillus.
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The complete genome sequence was determined by Illumina Solexa technology
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at the Beijing Genomics Institute (BGI) (Shenzhen, China). Assembly was performed
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using SOAP denovo v2.04 (4).
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The genome assembly of Bacillus sp. FJAT-14515 (G+C content of 37.06%) has
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approximately 98-fold coverage. It contains 28 scaffolds totaling 5,443,019 bp (largest,
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2,476,485 bp, and smallest, 598 bp). The scaffolds consist of 43 contigs totaling
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5,436,942 bp(largest, 793,370 bp, and smallest, 204 bp). N50 scaffold lengths of
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793,370 bp and N50 contig lengths of 435,981 bp were obtained. All assembly data
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were deposited in the DDBJ/EMBL/GenBank nucleotide sequence database.
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Coding sequences (CDSs) were predicted using Glimmer 3.02 (5) and further
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annotated using Uniprot, NCBInr, COG and KEGG through BLASTP. tRNAs, rRNAs
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and sRNA were identified using tRNAscan-SE (6), RNAmmer (7) and Rfam (8),
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respectively.
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The genome contains 5,263 CDSs with the average length of 789 bp that
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represent 76.5% of the whole genome. The result of annotation shows that only 837
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(15.9%) genes did not match any known protein in the current public protein
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databases. Of the 5,263 genes, 2,735 and 2,812 proteins were assigned into COGs
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functional categories and KEGGs, respectively. Additionally, 67 tRNAs, 31 sRNAs, 5
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rRNAs.7 CRISPRs were identitied in our genome.
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Nucleotide sequence accession number. This Whole Genome Shotgun
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project
of
Bacillus
sp.
(strain
FJAT-14515)
has
been
deposited
at
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DDBJ/EMBL/GenBank under the accession AYSD00000000. The version described
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in this paperis version AYSD00000000.
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ACKNOWLEDGMENT
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This work was supported by agricultural bioresources institute, Fujian Academy of Agricultural
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Sciences, PR China. The work was financed by the 948 project (2011-G25) from Chinese Ministry
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of Agriculture as well as by the 973 program earlier research project (2011CB111607), the project
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of agriculture science and technology achievement transformation (2010GB2C400220), the
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international cooperation project (2012DFA31120) from Chinese Ministry of Science and
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Technology,Natural Science Foundation of China (NSFC)(31370059), respectively.
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