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Supplementary materials
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3
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Table S1 Fatty acid content (%) of strain Ma-21T and related type species
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6
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Strains
1
2
3
4
5
6
7
C8:03OH
ND
ND
ND
ND
ND
ND
1.1
C14:0
2.2
ND
ND
1.1
ND
ND
C16:0
5.9
26.6
3.7
14.5
3.9
2.2
5.8
C18:0
ND
ND
2.0
11.1
ND
1.2
ND
C16:1ω11c
1.9
ND
ND
ND
1.0
ND
2.3
C16:1ω7c alcohol
1.5
ND
2.3
ND
2.2
1.4
4.0
C18:1ω9c
ND
ND
ND
ND
ND
ND
2.1
Iso-C12:0
ND
ND
ND
ND
2.9
Iso-C14:0
2.4
5.2
28.0
18.1
8.8
3.4
19.8
Iso-C15:0
5.8
10.8
2.7
8.3
10.6
20.0
25.4
Iso-C16:0
3.7
6.8
29.2
11.4
7.3
6.0
11.1
Iso-C17:0
ND
ND
1.3
2.7
1.7
Iso-C17:1ω5c
ND
12.4
ND
ND
ND
ND
6.6
Anteiso-C15:0
59.7
28.9
24.4
26.6
52.3
38.7
14.8
Anteiso-C17:0
13.8
7.0
5.3
3.0
8.9
19.9
2.4
Summed feature 4
2.8
ND
ND
7
1.8
2.4
Strains: 1, Oceanobacillus bengalensis Ma-21T; 2, O. pacificus XH204T; 3, O. chungangensis
CAU 1051T; 4, O. caeni S-11T; 5, O. profundus CL-MP28T; 6, Ornithinibacillus contaminans
CCUG 53201T; 7, Orn. heyuanensis GIESS003T. Summed feature 4: C17:1 iso I/anteiso B. All
data are from this study. “-” less than 1% of the total fatty acids, “ND” not detected.
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Fig. S1 Transmission electron micrograph of negatively stained cells of strain Ma-21T. Cells
were grown on Marine agar 2216 for 2 days at 30 °C.
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Fig. S2 Photomicrograph of sporangia and vegetative cells of strain Ma-21T. Ellipsoidal
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endospores are formed in subterminal or central positions in sporangia. Arrows indicate
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endospores.
63
Bacillus acidicola 105-2T(AF547209)
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Bacillus timonensis MM10403188T(JF824810)
89
50
97
Bacillus kochii WCC 4582T(ZFN995265)
Bacillus endophyticus 2DTT(AF295302)
Bacillus subtilis DSM10T(AJ276351)
Bacillus circulans ATCC 4513T(AY724690)
Ornithinibacillus contaminans CCUG 53201T(NR 118626)
Oceanobacillus picturae LMG 19492T(AJ315060)
Oceanobacillus manasiensis YD3-56T(FJ386518)
99
86
Oceanobacillus kapialis SSK2-2T(AB366005)
Oceanobacillus limi H9BT(HQ433455)
Ornithinibacillus bavariensis WSBC 24001T(Y13066)
Ornithinibacillus composti GSS05T(KJ868190)
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Ornithinibacillus scapharcae TW25T(AEWH01000025)
Ornithinibacillus californiensis MB-9T(AF326365)
Oceanobacillus polygoni SA9T(AB750685)
99
Oceanobacillus profundus CL-MP28T(DQ386635)
Oceanobacillus rekensis PT-11T(HQ620695)
Oceanobacillus damuensis PT-20T(HQ620704)
72
Oceanobacillus massiliensis NdiopT(HQ586887)
99
Oceanobacillus pacificus XH204T(JX501255)
Oceanobacillus halophilum GD01T(JX274441)
64
Oceanobacillus caeni S-11T(AB275883)
Ornithinibacillus heyuanensis GIESS003T(KF317693)
Oceanobacillus bengalensis Ma-21T(KF958190)
Oceanobacillus chungangensis KCTC 33035T(JX233492)
Oceanobacillus arenosus CAU1183T(JX233495)
99
Oceanobacillus kimchii X50T(GU784860)
99
Oceanobacillus iheyensis HTE831T(BA000028)
Oceanobacillus sojae Y27T(AB473561)
67
Oceanobacillus neutriphilus A1gT(EU709018)
88
97
98
Oceanobacillus oncorhynchi subsp. oncorhynchi R-2T(AB188089)
Oceanobacillus oncorhynchi subsp. incaldanensis 20AGT(AJ640134)
Oceanobacillus gochujangensis TK1655T(JN808225)
98
Oceanobacillus locisalsi CHL-21T(EU817570)
Oceanobacillus indicireducens A21T(AB623011)
Oceanobacillus chironomi LMG 23627T(DQ298074)
94
Oceanobacillus luteolus WM-1T(KC311561)
80
Paenibacillus polymyxa IAM 13419T(D16276)
0.05
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Fig. S3 Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the
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relationship between strain Ma-21T and close related type species. Numbers at nodes indicate
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percentage levels of bootstrap support based on a neighbour-joining analysis of 1000
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resampled datasets; only values >50% are given. Bar, 0.05 substitutions per site.
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Bacillus timonensis MM10403188T(JF824810)
Bacillus endophyticus 2DTT(AF295302)
Bacillus subtilis DSM10T(AJ276351)
75
Bacillus kochii WCC 4582T(ZFN995265)
93
Bacillus acidicola 105-2T(AF547209)
Bacillus circulans ATCC 4513T(AY724690)
Oceanobacillus picturae LMG 19492T(AJ315060)
Oceanobacillus manasiensis YD3-56T(FJ386518)
99
65
Oceanobacillus kapialis SSK2-2T(AB366005)
Ornithinibacillus contaminans CCUG 53201T(NR 118626)
Paenibacillus polymyxa IAM 13419T(D16276)
Ornithinibacillus bavariensis WSBC 24001T(Y13066)
Ornithinibacillus composti GSS05T(KJ868190)
Ornithinibacillus scapharcae TW25T(AEWH01000025)
Ornithinibacillus californiensis MB-9T(AF326365)
Oceanobacillus limi H9BT(HQ433455)
98
Oceanobacillus polygoni SA9T(AB750685)
Oceanobacillus profundus CL-MP28T(DQ386635)
89
Oceanobacillus damuensis PT-20T(HQ620704)
Oceanobacillus massiliensis NdiopT(HQ586887)
Oceanobacillus rekensis PT-11T(HQ620695)
Oceanobacillus indicireducens A21T(AB623011)
Oceanobacillus chironomi LMG 23627T(DQ298074)
84
62
Oceanobacillus luteolus WM-1T(KC311561)
Oceanobacillus pacificus XH204T(JX501255)
97
Oceanobacillus kimchii X50T(GU784860)
Oceanobacillus iheyensis HTE831T(BA000028)
53
Oceanobacillus neutriphilus A1gT(EU709018)
68
76
81
99
Oceanobacillus sojae Y27T(AB473561)
Oceanobacillus oncorhynchi subsp. oncorhynchi R-2T(AB188089)
Oceanobacillus oncorhynchi subsp. incaldanensis 20AGT(AJ640134)
Oceanobacillus gochujangensis TK1655T(JN808225)
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97
Oceanobacillus locisalsi CHL-21T(EU817570)
Ornithinibacillus heyuanensis GIESS003T(KF317693)
Oceanobacillus halophilum GD01T(JX274441)
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Oceanobacillus caeni S-11T(AB275883)
Oceanobacillus bengalensis Ma-21T(KF958190)
Oceanobacillus chungangensis KCTC 33035T(JX233492)
Oceanobacillus arenosus CAU1183T(JX233495)
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Fig. S4 Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing
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the relationship between strain Ma-21T and type species in related genera. Numbers at nodes
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indicate percentages of 1000 bootstrap resamplings, only values above 50 % are shown.
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Ornithinibacillus heyuanensis GIESS003T
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Orn. contaminans DSM 22953T
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Oceanobacillus bengalensis Ma-21T
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O. pacificus XH204T
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O. profundus CL-MP28T
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O. caeni S-11T
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O. chungangensis CAU 1051T
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Fig. S5 Partial 16S rRNA gene sequence chromatograms of strain Ma-21T and related type
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strains. The 16S rRNA genes from the genus Ornithinibacillus are more heterogenetic than
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those of the genus Oceanobacillus in this study.
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Fig. S6 Two-dimensional thin-layer chromatograms of polar lipids of strain Ma-21T,
Oceanobacillus caeni S-11T and Oceanobacillus profundus CL-MP28T. DPG,
diphosphatidylglycerol; PG, phosphatidylglycerol; NPL, ninhydrin-positive lipid;
NPPL, ninhydrin-positive phospholipid; PL, unidentified phospholipid; L,
unidentified polar lipid.