Gene & genome technology
Content
- Chemistry and biochemistry of nucleic acids
- RecDNA: enzymatic armamentorium and other tools for in vitro
construction and analysis.
- Cloning vectors and basal techniques of DNA cloning,
construction of DNA libraries.
- Expression strategies, hyperproduction and characterisation of
cloned proteins.
- Principles and applications of the polymerase chain reaction.
- Sequence analysis of informational macromolecules.
- Changing a genome, gene or gene-product: site-directed
mutagenesis and genome engineering.
- Exploration of genome and cDNA libraries.
- Applications in diagnostics, analysis of genetic & genomic
diversity, etc.
Interrelationships of genomics, proteomics, interactomics, metabolomics
Aims
The basic principles, techniques and strategies that are instrumental to molecular cloning and
genome analysis are described, in particular using Escherichia coli as host organism, and
including analysis techniques for genes and gene products. At an advanced stage, aspects of
analysis and exploration at the genome level are highlighted.
Previous knowledge
The student should have basic knowledge about the cell and cellular functioning, and about the
(bio)chemical constituents of an organism. Insight in the spatial structure of proteins and catalytic activity of
enzymes is expected. Essentials of metabolism and microbiology are particularly helpful.
Prerequisites: Cell biology, Chemistry, Biochemistry, Genetics