CURRICULUM VITAE
Dr. Pratibha Sharma
Field of specialization: Plant Pathology
Present Designation: Professorand Principal Scientist
Institute presently serving: Division of Plant Pathology,
Indian Agricultural Research Institute
New Delhi-12 (India)
Date of Birth: 23 May, 1954
Contact Information:
Division
of
Plant
Pathology,
Indian
Agricultural
Research Institute (IARI)
New Delhi-12
India.
Contact No.:
(a)Official:
Tel. No.: 011-25848418,
25843474
Fax No.: 011-25840772,
25843113
(b)
Residential:
Tel. No.: 011-25841442
E-mail:
psharma032003@yahoo.
co.in,
pratibha@iari.res.in
Permanent Address:
C-42, Pusa Campus,
Indian
Agricultural
Research Institute (IARI)
New Delhi-12
India.
SERVICE IN RELEVANT FIELD OF PLANT PROTECTION
(Plant Pathology)
Total Research Publications: 72
Total Books: 3 authored
Total Book Chapters/Manuals: more than 21
Total Conference proceedings/Popular articles/ Bulletins/Short
communications: approx. 100
Total Lead Lectures: 45
ACADEMIC RECORD
Degree
Board/University
Matriculation
1st Div.
Pre University Course
Secondary
Education 1969
Board, Rajasthan
Rajasthan University
1970
B.Sc.
Rajasthan University
1973
1st Div.
M.Sc.
Rajasthan University
1975
1st Div.
Ph.D.
Kanpur University
1992
-
1
Year
Division
1st Div.
PROFESSIONAL ATTAINMENTS
Employment Record & Experience (Starting from the present position)
Designation Pay Scale/
Nature of
work
Organization
Pay Band
Institution &
Period
Place of posting
(From-To)
Professor
and
Principal
Scientist
3740067000
Academic in
charge
Indian
Council of
Agricultural
Research
(ICAR)
Div. of Plant
Pathology, IARI, New
Delhi
Sept, 2010Continuing
Principal
Scientist
1640022400
Research
Indian
Council of
Agricultural
Research
(ICAR)
Div. of Plant
Pathology, IARI, New
Delhi
July, 1998Continuing
Senior
Scientist
3700-5700 Research
Indian
Council of
Agricultural
Research
(ICAR)
Div. of Plant
Pathology, IARI, New
Delhi
June 1997- July
1998
Senior
Scientist
3700-5700 Research
and
management
Indian
Council of
Agricultural
Research
(ICAR)
Planning, Publication
and Information Unit
of Directorate, IARI,
New Delhi
April 1995- May
1997
Senior
Scientist
3700-5700 Research
Indian
Council of
Agricultural
Research
(ICAR)
Division of
Floriculture, IARI,
New Delhi
April 1988- June
1997
Senior
Scientist
3700-5700 Research
Indian
Council of
Agricultural
Research
(ICAR)
Directorate of Pulses
Research, Kanpur
Jan, 1986- March
1988
Scientist
S-II
1100-1600 Research
Indian
Council of
Agricultural
Directorate of Pulses
Research, Kanpur
April 1985–
Dec.1986
1
Research
(ICAR)
Scientist
S-I
700-1300
Research
Indian
Council of
Agricultural
Research
(ICAR)
IARI Regional Station,
Kanpur
April 1979March1985
Scientist
S-I
700-1300
Research
Indian
Council of
Agricultural
Research
(ICAR)
Div. of Plant
Pathology, IARI, New
Delhi
Dec, 1976- March
1979
Scientist
S-I
700-1300
Research
Indian
Council of
Agricultural
Research
(ICAR)
NAARM, Hyderabad
Sept, 1976- Dec,
1976
IN-SERVICE AWARD/RECOGNITION
Name of the Awarding
Award/
Organization
Recognition
(Place / country)
General
Secretary
Member,
Councilor
Year
National/
International
Institutional/
Professional
Society
Crop
Protection 2012Professional
Federation, New Delhi contd.
SocietiesNational
International Society of 2012-13 International
Plant Protection
Best Citizens of International
2011
India
Publishing House, New
Delhi
SAB Women Society for Applied 2011
Scientist Award Biotechnology, Tamil
Nadu-635201
National
K.C. Mehta and Indian
National
National
2011
1
Additional Information
For
the
interaction
at
international and national level.
Interaction at the international
level as a secretary of Indian
Phytopathological Society ,
New Delhi
For contribution to agricultural
research.
For outstanding contributions to
the field of agricultural research
through Plant protection and
Organic farming technologies.
For best Paper “Susanta Banik
Manoranjan
Mitra Award
Phytopathological
Society, IARI, New
Delhi
Indian Society
of Ornamental
Horticulture
(ISOH)
Fellowship
Member
of
Expert
committee (EC)
for S&T for
Women”
Member
of
National
Extension
Programme
Outstanding
Women
Scientist Award
Indian
Society
of 2011
National
Ornamental
Horticulture,
IARI,
Pusa Campus, New
Delhi-12
Ministry of Science and 2011-14 National
Technology, DST, New
Delhi
Secretary
Member
Board
management
Expert
IARI, New Delhi-12
National
conference,
Unraveling
multiple
facets of Biological
control in National
purview:
Trends,
challenges and societal
implications and Indian
Phytopathological
Society Zonal Meeting,
Mid-eastern
zone.
Banaras
Hindu
University, Banaras
Indian
Phytopathological
Society, IARI, New
Delhi
of NCIPM, New Delhi
of
and Pratibha Sharma (2011).
Plant pathology in the era of
nanotechnology.
Indian
Phytopathology 64: (2) 120127”
For contribution to Floriculture.
For
reviewing
and
implementing projects in rural
areas / weaker sections.
2010Contd.
National
For demonstration of
protection Technologies.
2010
National
For the field of Vegetable
disease management using ecofriendly means.
20102011
National
Execution of the programmes
related to the society
20112013
National
For research programmes
Planning
Commission,
Preparation of the document by
Working Groups ( V )
Working group V on 2006
Value
addition/
1
Crop
Women
Scientist
Felicitation
Member
Member
Infrastructure/Organic
Farming
of
Agro
Climatic
Zonal
Planning for eleventh
five year plan (20072012)
World Academy of 2006
Ayurveda, India
Government
of India
World
Academy of
Ayurveda
Institutional
Dissemination of Biopesticide
and
organic
farming
technology, IPM In Farmers
Selection of Ph. D students
2006
National
To discuss the current status of
IPM and Biopesticides ,and to
formulate the future programme
2006
National
Monitoring of the preparation
Of Pesticide Encyclopedia to
fine tune the scope, coverage
and budget for the study in
consultation
with
the
Implementing Agency
20062007
National
Execution of the programmes
related to the society
20062007
Institutional
For teaching, research
students activities
2006
National
To develop a pro community
technology resource village in
Hilly
areas
and
for
empowerment of Scheduled
Castes in Hilly region
PG School of IARI, 2006
New Delhi
Brainstorming
on
Integrated
Pest
Management
and
Biopesticides NCIPM,
IARI, New Delhi.
Member
Monitoring Committee
of
the
Pesticide
Encyclopedia
Of
Technology
Information,
Forecasting
and
Assessment Council
( TIFAC ), DST
Treasurer
Indian
Phytopathological
Society, IARI, New
Delhi
Member
Board
of
Studies,
Division
of
Plant
Pathology, IARI, New
Delhi.
Chairman
A
Sensitization
and also expert Workshop
on
SC
member of the Resource
Village.
review
Department of Science
committee.
and Technology (DST),
New
Delhi
and
Himalayan
1
and
Environmental Studies
&
Conservation
Organization (HESCO)
Dehradun Uttaranchal
Dr.
M.
R. Bioved
Research
Siddiqui Medal Society, Allahabad
2005
National
For the work on Integrated Pest
Management and Biopesticides
Indian
2004
Phytopathological
Society, IARI, New
Delhi.
Society
for
the 2003
Protection
of
Rishi Award
Environment
and
sustainable
Development,
BHU,
Varanasi
G. D. Verma Society of Mycology 2003
Women
and Plant Pathology,
Scientist
Udaipur
Commendation
Award
Bioved
Research 2002
Fellowship
Society, Allahabad
Award
National
Selection
National
For plant disease management
(Honor of distinction)
National
On the work of Biological
Control
National
For outstanding contribution in
the field of science and
agricultural Research
To monitor, review and
improvise
the
extension
activities of IARI
Evaluation , selection and
approval of women candidates
for women fellowship
Evaluation and approval of
projects
Monitoring of the project in 12
states
Fellowship
Member
Extension Council of 2002
Institutional
IARI., New Delhi
onwards
Expert
Committee of Women
Fellowship Scheme
( WOS-B DST)
Committee for SC&
ST of DST.
Committee
in
the
Monitoring Committee
of the Net work project
of Fodder Production
and Seed for Special
Component Plan for
Scheduled
Castes,
Expert
Expert
20022007
National
20022007
20022007
National
National
1
Expert
Fellow
DST.
National
Steering 2002Committee for the 2007
evaluation
of
the
projects of Science and
Society of DST
Society
of
Plant 2001
Protection
Sciences,
New Delhi
National
Selection,
Recommendation,
and Approval of the projects
National
For outstanding contribution in
the field of science and
agricultural Research
INTERNATIONAL VISITS
Country
Type of Recognition
Year
Duration Funding agency
Ptlanzenschutz
Advanced studies for
in Ackerbau
host pathogen interaction
Grunland
and training in Phytotron
(BBA), Germany
1st September
1993 to 29th
February 1994
Six
months
UNDP- FAO Fellowship
Honolulu,
Hawaii, United
States
of
America.
To attend American
Phytopathological
Society (APS) and
International Association
for the Plant Protection
Sciences (IAPPS) Joint
Meeting, 2011
6-10th August,
7 days
Department of Science &
Technology, Govt.of
India, New Delhi
Chiangmai,
Thailand
To attend International
Conference on
Biopesticides ICOB 6
11 – 16
December,
2011
8 days
Stress Tolerant Rice in
Africa And South Asia,
International Rice
Research Institute, India
Office
Lincoln
University, New
Zealand
To attend 12th
International
Trichoderma and
Gliocladium Workshop
27 – 30
August, 2012
4 days
Stress Tolerant Rice in
Africa And South Asia,
International Rice
Research Institute, India
Office
2011
1
EXTERNALLY FUNDED PROJECTS
Pl or Co-Pl/
Associated
Scientist
Title of Project (s)
Period
(From-To)
Amount/ Funding agency
Budget
Co-PI
“Production of cut flowers
under Protected
Conditions
(Roses,
Carnation
and
Gerbera).
Development of Disease and
Insect Pests resistant superior
varieties of cabbage and
cauliflower.
Development
of
hybrids
resistant to multiple disease
/pests.
Development of Pesticide
Residue Free IPM Packages
for Vegetables.
Expert system of Extension
(NATP-CGP)
for
major
vegetables
tomato,
peas,
ornamentals gladioli.
1999-2002
15.0
Indian Council of
Agricultural Research
(ICAR), New Delhi
1999- 2004
97 .0
NationalAgriculture
Technology Project
(NATP), ICAR,New Delhi
1999-2004
32.0
2000-2004
37.0
2001-2004
24.0
PI
Development
of 2002-2005
bioformulation from improved
strains of Trichoderma for
cauliflower and tomato.
15.0
PI ( IARI,
Centre,
Vegetables )
National Network project for 2004-2007
“Management for Alternaria
blight of Brassica juncea and
vegetable crops” (NSS-8).
National Network project on 2004-2007
wilt of crops with special
reference
to
cultural,
morphological,
molecular
characterization
and
pathogenic
variability
of
isolates of India. (NSS-9).
Referral laboratory for the 2004-2007
testing of Biopesticides.
11.13
NationalAgriculture
Technology Project
(NATP), ICAR, New Delhi
NationalAgriculture
Technology Project
(NATP), ICAR, New Delhi
NationalAgriculture
Technology Project
(NATP), ICAR, New
Delhi- Competetive Grant
Programme
NationalAgriculture
Technology Project
(NATP), ICAR, New
Delhi- Competetive Grant
Programme
Indian Council of
Agricultural Research
(ICAR), New Delhi-12
Technology
Vision
project titled: On
Demonstration
39.0
Co-PI
Co-PI
Co-PI
Expert
PI( IARI,
Centre
Vegetables )
Co-PI
PI
2020 2008-2012
Farm
and
1
9.15
Indian Council of
Agricultural Research
(ICAR), New Delhi-12
6.0
Department of
Biotechnology (DBT), New
Delhi
Technology, Information,
Forcasting and Assessmemt
Commercial Cultivation of
Trichoderma as Bio-Pesticide
& Growth promoter.
PI
PI
PI
Associate
Co-PI
Associate
Outreach
Programme
on
“Diagnosis and Management
of Leaf spot diseases of field
and horticultural crops in
vegetables.
Contract Research entitled:
Testing of Bio-efficacy of
Formulation
SAS-102“ROOT-O-MAX”
on
Vegetable: Cauliflower Crop.
Contract research project on
“Research on High Density
CFU Microbial Formulation”.
Livelihood and Nutritional
Security of Tribal Dominated
Areas
through
Integrated
farming
System
and
Technology Models.
Niche Area of Excellence’s
project entitled “Exploration
and
Exploitation
of
Trichoderma as antagonist
against soil borne pathogens”
Council
(TIFAC)Department of Science and
Technology (DST), New
Delhi.
2009-2012
49.0
Indian Council of
Agricultural Research
(ICAR) -network
Nov, 2010April, 2011
1.5
Contractual-Jayshree
Rasayan Udyog, Limited
2011continuing
6.8
October
2007-12
31.0
Contractual- International
Panacea Limited,New
Delhi
National Agricultural
Innovative Project (NAIP),
ICAR
2012-2016
onwards
<45.0
Use of Trichoderma against
abiotic stresses in “Stress
tolerant rice for Africa and
south Asia (STRASA) Phase
2.”
2011onw
ards
>5.0
CSAU, Kanpur, Indian
Council of Agricultural
Research –Education and
Indian Agricultural
Research Institute
International Rice Research
Institute (IRRI), New Delhi
Office
SPECIAL
ATTAINMENTS
&
ACHIEVEMENTS
OF
PRACTICAL
IMPORTANCE
(Patents, technologies, varieties, products, concepts, methodologies etc.)
Category
Title
Multilocation
trials for disease
resistance
Multi-location
evaluation
pigeonpea
Year
Individual/
Collaborative
1980of 1988
Additional
Details/Information
Collaborative
A national Sterility Mosaic
(Amin-KS; Reddy- Disease
nursery
was
MV;
Nene-YL; developed to evaluate the
1
( pulses )
Raju-TN;
pigeonpea material received
from all centres
PratibhaShukla;Zote-KK;
Arjunan-G; BendreNJ;
Rathi-YPS;
Sinha-BK; GuptaRP; Anilkumar-TB;
Chauhan-VB;
Bidari-VB;
Gurdeep-Singh;
Jha-DK; KausalyaGanghadharan;
Shukla-P; Singh-G;
Ganghadharan)
(Cajanus cajan)
for
broad-based
resistance
to
sterility-mosaic
disease in India. In
collaboration with
ICAR, ICRISAT,
Agriculture
Universities –K.
(1993)
Identification of
Sterility Mosaic
resistance in
Pigeonpea with P.
Sharma and A. N.
Asthana
Identification of
powdery mildew
resistance in peas
and rust of lentil P.
Sharma and. S. P.
Mishra
Disease resistant In the collaborative 19882002
varieties
with work of
good
flowering development of
following new
characters
Hybrid Tea
(flori-culture)
varieties with the
Rose Breeders:
Chitra (1995), Dr.
Benjamin Pal
(1993), Mother
Teresa (1994),
Raktima (1991),
Preyasi (1991),
Shreyasi (1991)
Floribunda
Varieties: Dr. S. S.
Bhatnagar (1994),
Lahar (1991),
Manasi (1991).
Climbers:
Climbing
Collaborative
(Singh, A. P., B.
Singh
and
P.
Sharma.
1999.
Performance
of
some new IARI
roses. The Indian
Rose Manual XVI
pp. 44 – 47)
1
This work was suggested by
Late Dr. B. P. Pal who
desired
to
incorporate
disease resistance in roses
also.
latest other seven
varieties of Roses
tolerant
to
powdery mildew
and black spot in
2001 and 2004
(Pusa
Mansij,
Pusa Mohit, Pusa
Abhishek, Pusa
Manhar,
Pusa
Muskan,
Pusa
Urmil and Pusa
Ranjana)
(18 varieties )
Singh, A.P, K.P.
Singh, K.V. Prasad
and
Pratibha
Sharma.2005.New
roses from IARI,
New Delhi.. Indian
Horticulture.
Vol.50 (3): 8-9
Techniques for
multistoreyed
budding in roses.
A. P. Singh, B.
Singh and Pratibha
Sharma. A study
on multistoried
budding in roses
The Indian Rose
Annual XVI pp.
86-89.
Collaborative
Selection of Zinnia
Leaf Curl
resistance
“Production of cut
flowers under
Protected
Conditions (Roses,
Carnation and
Gerbera
Disease resistant With vegetable
breeders:
varieties
(Vegetable crops). Development of
leaf curl and
Alternaria blight
resistant varieties
of
tomato,phomopsis
blight of
brinjal,purple
blotch of onion.
Multiple disease
resistant varieties
of cabbage and
19982005
Collaborative
(S.P.S. Raghawa
and P. Sharma)
H.P.Singh, B.Singh
and P. Sharma
1
IN ICAR Cess Fund Project
Divisional
NATP
projects
and
IPM Technology
Provided
Plant
protection
technology
for
IARI Website
Selection
and
improvisation of
Trichoderma
harzianum and T.
viride strains for
development of
bioformulation
Also worked on
the variability in
Trichoderma
strains
cauliflower
(Sclerotinia, Black
Rot, Alternaria
and Downy
Mildew) (NATP
1999-2004)
Development of
2000Pesticide Residue
2003
Free IPM packages
of vegetables:
Tomato, Chilliest
and Cabbage
Associated with the
disease resistance
programme
Development of
2000website http: //
2004
www.iasri.res.in:8
081/ krishnamadan
/ index. Html of
IARI by the PI Dr.
Ram Bahl and CoPI (Dr. M. Wasan,
Sh. S. Marwah).
Composition for 2005
early
profuse
sporulation under
solid state of the
improvised isolate
of
Trichoderma
harzianum and a
process thereof
Survey conducted 2004Collection,
identification and in different states 2007
Collaborative
Inter institutional
(IIHR Bangalore,
IIVR Varanasi.
Division of
Entomology and
Agricultural
Chemicals, IARI)
Team Work: G.
Kulsreshtha , M.
Gopal, P. K.
Malhotra, P.
Sharma, I.
Mukherjee, S. B.
Singh , Ranbir
Singh, S. Chandra)
Collaborative
Inter institutional
NATP/PSR-41/RW/6.6/71/19
Individual
Patent Application number
5920/RQ-Del/2006
(published) This work was
conducted CGP-399NATP
Development
of
bioformulation
from
improved
strains
of
Trichoderma
for
cauliflower and tomato.
This patent is based on the
improvised
strain
of
Trichoderma
harzianum
under CGP- NATP
National Network project
on wilt of crops with special
Collaborative
Inter institutional
1
As an expert resource
persons for the Plant
Protection Technology of
Tomato, pea, and Gladiolus.
Provided crop protection
technology
determination of
pathogenic and
Molecular
variability
in
Fusarium
oxysporum f.sp.
cucumerinum
(Cucumber and
water melons )
to assess the losses
in vegetable crops,
collection
of
diseases samples,
their identification
and
purification
and preparing the
passport data for
all the collected
isolates.
Pathogenic
variability
and
molecular
characterization
of
Alternaria
brassicae,
A.
brassicicola
(Cauliflower) and
A. porri (Onions)
Characterization of
variability
and
develop
management
practices mainly
Biocontrol and non
chemical methods
Development of
IPM and IDM
packages in both
the projects
Two locations
2005simultaneously i.e. 2009
village around
Delhi (Palahari
Sonepat district of
Haryana) and
RAU, ARS,
Durgapura to
generate location
specific IPM
Technology
Study on
2007
corroboration
between DNA
markers (RAPD,
ISSR, ITS) and
bio-control
efficacy of
Trichoderma
species
Development and
validation of IPM
Technology
in
Vegetable Crops
(Cauliflower and
Cabbage)
Characterization
of fungal bio
agents.
Developments of Studies on the
variability of
ecofriendly
Sclerotinia
disease
reference
to
cultural,
morphological, molecular
characterization
and
pathogenic variability of
isolates of India. (NSS-9)
2007
Collaborative
Inter institutional
National Network project
for
Management
for
Alternaria
blight
of
Brassica
juncea
and
vegetable crops (NSS-8)
Collaborative
Inter institutional
IARI
and
NCIPM
collaboration
As a plant pathologist and
biocontrol
scientist
collaborated on supplying
strain
of
Trichoderma
harzianum against soil and
foliar diseases.
PhD Programme
Twelve
isolates
of
Trichoderma
harzianum
and Trichoderma viride
were assessed for their
mycoparasitic effect on
phytopathogens
Pythium
aphanidermatum
and
Sclerotinia
sclerotiorum.
And
also
molecularly
characterized.
The IDM package was
developed
for
organic
farmers by using plant
PhD Programme
1
sclerotiorum
causing rots in
cauliflower and it,
non chemical
management.
Specific
PCR- 2007
Molecular
based marker for
characterization
of
of
Fusarium detection
oxysporum f.sp. pathogenic
groups
of
cucumerinum
causing wilt in Fusarium
oxysporum f. sp.
Cucumber.
cucumerinum
in
India
extracts and Trichoderma
harzianum (Th3).
management
package
for
Scerotinia rot in
cauliflower.
Development of
biological
formulation
of
suitable strain of
Pseudomonas
fluorescence
against
Rice
diseases.
Studies on the
carrier and
ecological
colonization
PhD Programme
2008
PhD Programme
1
For the detection of
Fusarium oxysporum f. sp.
cucumerinum
pathogenic
groups, specific PCR-based,
random
amplified
polymorphic DNA (RAPD)
markers which identified in
four pathogenic groups I, II,
III, and IV were cloned into
Pgem-Teasy Vector. Cloned
fragments were sequenced,
and used for developing
sequence
characterized
amplified regions (SCAR)
primers for detection of
pathogenic
groups.
F.
oxysporum
f.
sp.
cucumerinum
isolates
belonging
to
four
pathogenic groups in India,
cucumber nonpathogenic F.
oxysporum, F. oxysporum f.
sp.
moniliforme
and
melonis, Fusarium udum,
and isolate of Alternaria sp.
were tested using developed
specific primers.
Bacterial
antagonists
Pseudomonas fluorescence
have been proved to be
highly effective against
Bacterial blight of rice
caused by Xanthomonas
oryzae pathovar. Oryzae,
Pseudomonas fluorescence
and Bacillus subtilis were
tested against Xanthomonas
campestris pv. Campestris
and
Ralstonia
solonacearum
reduced
disease incidence in field.
Development of
liquid
formulation
against
foliar
diseases
with
special reference
to Purple blotch
of Onion and
Alternaria blight
of
Cruciferous
vegetables
Demonstration of
low
cost
techniques
for
effective
crop
protection
through
Trichoderma bioformulations
through IPM in
selected villages
in two different
districts
of
Rajasthan.
To
develop
replicable
entrepreneurship
model
for
application and
commercial
production
of
Trichoderma.
Liquid formulation
of this strain has
been developed
and tested against
the foliar
pathogens and also
integrated in IDM
package of the
disease.
20082012
Divisional, ORP and Ph.D
programme. This helps to
provide a non chemical
strategy.
Demonstration of 2008the Trichoderma 2012
technology
by
introducing
bioformulation in
the fields of 20
villages of two
districts
of
Rajasthan covering
32 crops, 188
farmers.
Economic analysis
of the Groundnut
data showed higher
economic returns
and benefit-cost
ratios in
application of Th3
(Rs 119293/ha,
1:6.5) as compared
to farmers practice
(Rs 77632 /ha, 1:
4.8).
Two
on
field
training
programme were
organized on 15th
October to 20th
October 2011 at
Gramin
Vigyan
Kendra –Digodh,
Kota, and on 21st
October to 31st
October 2011 at
Samode
and
Individual
1
Department of Science and
Technology
(DST)technology,
Information,
Forecasting
and
Assessment
Council
(TIFAC) project “On Farm
Demonstration
&
Commercial Production of
Trichoderma
as
Biopesticide
&
Growth
Promoter”
Pathogenic
variability
of
A.brassicae
causing leaf spot
of
cruciferous
vegetables
and
mustard
crop.
IDM package.
Cross infectivity
studies of
A.brassicae and
A.brassicicola
isolates on
different oilseed
and vegetable
crucifers.
Development of
Specific marker
for detection of
black leaf spot
Dodhsar VillageJaipur.
Kisan mela was
organized at
Dodhsar village,
Jaipur district on
1st November,
2011.
Thirty two
2009A.brassicae
2013
isolates infecting
both cauliflower
and mustard were
molecularly
characterized using
RAPD, ISSR and
ITS-RFLP primers.
The result with no
inter strainal
variation among
the isolates
indicate genetic
homogeneity
among the
A.brassicae
isolates.
Some exceptional
results were
observed where
mustard isolates of
A.brassicae could
infect Cauliflower
leaves but some of
the Cauliflower
isolates of
A.brassicae were
ineffective on the
mustard crop.
Whereas all the
A.brassiccicola
isolates were found
to infect all crops.
Detection of Seed
borne infection in
cruciferous seeds.
collaborative
ICAR Outreach programme
in
Network
mode
“Diagnosis
and
management of leaf spot
diseases of field and
horticultural crops”
A
polymerase
chain
reaction based assay was
developed for the detection
and identification of A.
1
disease caused by
Alternaria
brassiccicola
brassicicola using specific
primers designed from
conserved
nuclear
ribosomal
internal
transcribed spacer (ITS)
regions. The specificity of
the primer developed was
evaluated for pure culture
and seeds infected with
A.brassicicola.
The
sensitivity of the designed
primer set and its lowest
detection limit (100pg) was
verified
through
quantitative Real Time
PCR.
ITS and Endochitinase gene
sequences of Alternaria
brassicae, A.brassicicola,
A.porri, Trichoderma sps.,
Fusarium
oxysporum
cucumerinum
This
will
help
in
commercialization of the
strain as bioformulation.
Department of Agriculture,
Durgapura, Jaipur will
purchase the technology
Deposition of
sequences in
NCBI
More than 100
sequences were
submitted to
Genebank, NCBI
2004
onward
s
Individual
Toxicological
testing of
Trichoderma
harzianum Th3
The T.harzianum
strain Th3 has
been submitted for
toxicological
testing to National
Toxicological
Centre, Pune
Biocontrol strategy
for the
Management of
threatening
Diseases
201112
Individual ,
Divisional and
TIFAC- DST
project
200914
Divisional project
A
full
length
of
endochitinase gene for
Trichoderma
harzianum,
T.viride, and T.atroviride
was amplified using pair of
specific primers.
Ecological
colonization
studies on the
fungal and
bacterial bioagents
2008onward
s
Individual
Department of
Science and
Technology (DST)technology,
Information,
Forecasting and
Assessment Council
(TIFAC) project
Field
performance
of
bioformulation
of
Trichoderma
harzianum
(Th3), was evaluated in
terms of its rhizosphere
colonization
and
competence, survivability
(root colonization behavior)
and non-host specificity in
Mechanism of
action of
biocontrol agents
and
characterization
of bio-molecules
and genes
governing them
Survival and
persistence
studies of
biocontrol agents
in different agro
ecosystems
1
Demonstration of
Biocontrol
technology in
Guava wilt
affected area in
Rajasthan
Rejuvenation of
Guava wilt
affected area.
201112
SUMMER/WINTER
SCHOOL/
CONFERENCE ETC.
In collaboration
with farmers
REFRESHER
different crops including
cereals
(Rice,
Wheat,
Maize, Pearl millet, Barley),
Legume (Chickpea), oilseed
crops (Groundnut, Soybean,
Mustard,
Linseed
),
Vegetables
(Cauliflower,
Brinjal, Okra, Pea, Potato,
Tomato, Chilli, Garlic,
Onion,
Coriander,
Fenugreek),
Ornamental
crops (Rose, Marigold) and
fruit (Watermelon) of both
Rabi and Kharif season.
Department of Science and
Technology
(DST).
Bioformulation
of
T.harzianum
and
Aspergillus niger were
applied to Guava fields
affected with Wilt disease at
different villages of Ajmer
and Sawai Madhopur.
COURSE/
Name of the Function(s)
programme
performed
organized
Title
Year
National
Training
programmes
National
Training
programmes
Training
Associates
Phytotronics for
Agricultural
Research
Identification of
Plant Pathogenic
and Biocontrol
fungi
16-21
6 days
November,
1998
14th Sept.
30days
to 13th
Oct.2000.
National
Training
programmes
Course Leader
Associate
Duration Funding agency
Challenges and 16 Oct. to 21 days
Opportunities of 5
Nov.
Biological
2001
Control in plant
disease
management
1
SYMPOSIA/
UNDP & ICAR
Centre of
Advanced Studies
in Plant
Pathology, IARI,
New Delhi-12
Centre
of
Advanced Studies
in
Plant
Pathology, IARI,
New Delhi-12
National
Symposium
Organizing
Committee
National
Training
programmes
Course Leader
National
Seminar and
Zonal
Symposium
As Zonal
President of
Indian
Phtopathological
Society
National
Training
programmes
Associate
National
Training
programmes
Associate
National
Training
programmes
Course Leader
National
Symposium
on
Agriculture
in
Changing Global
Scenario
organized
by
Indian Society of
Agricultural
Sciences
and
Indian Council of
Agricultural
Research,
New
Delhi
Biocontrol
strategies
for
management of
plant pathogens
Feb. 21- 3 days
23. 2002
Indian Society of
Agricultural
Sciences
and
Indian Council of
Agricultural
Research, New
Delhi
16 Nov. 6 21days
Dec, 2004
National Seminar
and
Zonal
Symposium
of
Indian
Phytopathological
Society,
New
Delhi
“
Variability
in
plant pathogens
and
their
management”
“Entrepreneurship
development in
mass production
and
use
of
biological control
agents
in
agriculture
systems
“Biological
Software
and
Rural
Technologies’
Biological control
of
plant
pathogens
9
Dec, 1 day
2004
Centre
of
Advanced Studies
in
Plant
Pathology, IARI,
New Delhi-12
Zonal President
of IPS
1
16.11.0415.12.04
30 days
Division of
Entomology
and NCIPM,
New Delhi
1.11.04 – 30 days
31.12.04
PG School, IARI,
New Delhi
1/08/07 to 21 days
21/08/07
Centre
of
Advanced Studies
in
Plant
Pathology, IARI,
New Delhi-12
International
As an executive
symposium (IPS) member of IPS
5th International 10th to 13th 4 days
Conference
on Nov’09
Plant Pathology
in the Globalized
Era by Indian
Phytopathological
Society.
Indian
Phytopathological
Society,
New
Delhi-12,
and
Division of Plant
Pathology, IARI,
New Delhi-12
National
Symposium
(IPS)
Secretary IPS
Molecular
Approaches for
Management of
Fungal Diseases
of Crop Plants.
2-5
December,
2011
4 days
Indian
Phytopathological
Society, New
Delhi-12 and
Department of
Plant Sciences,
School of Life
Sciences,
University of
Hyderabad,
Hyderabad,
Andhra Pradesh.
National
Symposium
(IPS) 2012
Secretary IPS
Blending
3-6
Conventional and December,
Modern
Plant 2012
Pathology
for
Sustainable
Agriculture,
3 days
Indian
Phytopathological
Society and IIHR,
Bangalore
1
M.SC / PH.D STUDENTS GUIDED AS MAJOR/CO-GUIDE
1.
2.
3.
4.
Studies on Powdery mildew of roses.
Study of biotic and abiotic elicitors in cauliflower against downy mildew.
Interaction of Trichoderma harzianum with common pesticides.
Integrated Disease Management of Stalk rot of Cauliflower caused by Sclerotinia
sclerotiorum.
5. Studies on the hypocreal stage of Trichoderma and its molecular characterization.
6. Studies on the variability of Sclerotinia sclerotiorum causing rots in cauliflower and it,
non chemical management.
7. Molecular characterization of Fusarium oxysporum f.sp. cucumerinum causing wilt in
Cucumber.
8. Moelcular Characteization of Rhizoctonia solani causing sheath blight of rice.
9. Biological control of Bacterial blight of rice using Pseudomonas fluorescence.
10. Characterization and Management of Alternaria porri incitant of Purple blotch of Onion.
11. Study on Population dynamics and effect of Pesticides of Pseudomonas fluorescence and
Trichoderma harzianum used as an antagonist against bacterial blight of Rice in IPM
system.
12. Biochemical and Molecular Characterization of Alternaria brassicicola (Schwein)
Wiltshire causing black leaf spot of Cauliflower in India.
13. Epidemiology and diversity of Alternaria brassicae (Berk) Sacc. The causal agent of
black leaf spot in crucifers.
14. Effect of Abiotic stresses on biocontrol agent Trichoderma and their mechanism of
tolerance to these stresses.
15. Study on Population dynamics and effect of Pesticides of Pseudomonas fluorescence and
Trichoderma harzianum used as an antagonist against bacterial blight of Rice in IPM
system.
16. Biochemical and Molecular Characterization of Alternaria brassicicola (Schwein)
(Wiltshire) causing black leaf spot of Cauliflower in India.
17. Epidemiology and diversity of Alternaria brassicae (Berk) Sacc. The causal agent of
black leaf spot in crucifers.
18. Effect of Abiotic stresses on biocontrol agent Trichoderma and their mechanism of
tolerance to these stresses.
19. Morphological and taxonomical diversity in Powdery mildew pathogens of cucurbits in
northern India.
20. Cloning, characterization and expression of chitinase gene from Trichoderma spp
effective against sheath blight (Rhizoctonia solani Kuhn.) and blast (Magnaporthe
grisea) diseases of Basmati Rice.
In the advisory committee of the students from Schools of Crop Protection and Crop Production
1
WORK ON BIOCONTROL:
Projects undertaken:
 Development of Disease and Insect Pests resistant superior varieties of cabbage and
cauliflower. (Co-PI), 1999- 2004, (NATP Funding)
 Development of hybrids resistant to multiple disease /pests. (Co-PI), 1999- 2004 (NATP
Funding)
 Development of Pesticide Residue Free IPM Packages for Vegetables. (Co-PI), 20002004 (NATP Funding)
 Development of bioformulation from improved strains of Trichoderma for cauliflower
and tomato. (PI), 2002-2005, (NATP-CGP Funding)
 Technology Vision 2020 project titled: On Farm Demonstration and Commercial
Cultivation of Trichoderma as Bio-Pesticide & Growth promoter. (PI), 2008-2012,
(TIFAC-DST funding)
 Niche Area of Excellence’s project entitled “Exploration and Exploitation of
Trichoderma as antagonist against soil borne pathogens” To work for basic research.
(Co-PI), 2012-2016, (ICAR funding).
Major Output:









Developed Biological Formulation of Trichoderma harzianum and Pseudomonas
fluoresence.
Strainal variability in fungal bioagents with special reference to Trichoderma.
Development of pesticide resistant Trichoderma strains used for biodegradation,
Development of suitable techniques for bioefficacy and their application in field IN IPM
system.
Production of Trichoderma bioformulation in mass scale
On farm demonstration of Trichoderma technology
Entrepreneur development in Biocontrol technology
Characterization of major genes of biocontrol in Trichoderma spp.
Managed severe disease problems in Rajasthan with different agroclimatic zones of Kota
and Jaipur.
1
SIGNIFICANT ACHIEVMENT S IN BIOLOGICAL CONTROL
Development of bioformulation from improved strains of Trichoderma for cauliflower and
tomato. (NATP CGP III /399 ON)
To develop biologically active and effective strains of Trichoderma spp. more than 50
Trichoderma harzianum and T.viride isolates were isolated from the soil/plant samples collected
from different Delhi and adjoining areas. Many in vitro screening methods for antagonistic
potential have not predicted well in IPM system / and or farming system / although reliable
screening method can be a great benefit to a biological control programme by providing a cheap,
standardized method for candidate microorganism. Keeping this in view twelve isolates of
Trichoderma spp. including eight of Trichoderma harzianum and four of T. viride were
evaluated for developing methods for screening antagonists against multiple pathosystem.
Besides using dual test, the isolates were rigorously tested by using their toxic culture filtrate
against phytotoxic culture filtrate of ten pathogens to find out the possible detoxification of
several plant pathogens namely Colletotirchum capsici, Sclerotinia sclerotiorum, Pythium
aphenidermatum, Fusarium oxysporum f.sp lycopersici, Alternaria brassisicola, A.solani, A.
alternata Phomopsis vexans, Rhizoctonia bataticola and Rhizoctonia solani isolated from chilli,
cauliflower, tomato and brinjal plants . The Trichoderma spp. isolates were grown on a medium
containing the above mentioned pathogen toxins at different concentration. These treated toxins
were tested for its phytotoxic activity using symptom bioassay on their respective host plant
leaves/seedling. Trichoderma treated phytotoxins exhibit very less symptom development in
comparison to pure phytotoxin. It was observed that Th3, Th10, Th30, Th31 and Th32was found
effective even at 1:3 ratios of Trichoderma metabolites and phytotoxin to reduce the diseases
symptom on leaves and seedlings. The result shows that the effective isolates possessing the
biocontrol characteristic cause detoxification of different pathogen toxins, which is one of the
major pathogenicity determinants of the infection by the pathogen. This process protects plants
from pathogen infection due to development of resistant/hypersensitive reaction.
These isolates were also screened against the commonly used pesticides i.e. benomyl,
carbendazim, thiram, captan, mancozeb, iprodione, imidacloprid, chlorpyriphos, and
pendamethalin and evaluated in terms of sensitivity EC50 scale using three scales (i) low risk
pesticide (> 100ug/ml) (ii) medium risk (1 -100ug /ml) and (iii) high risk (0.1-1ug / ml) based on
growth and sporulation intensity and categorized as follows, low risk pesticides captan and
mancozeb, medium risk benomyl, iprodion, thiram,pendimethalin and ,high risk carbendazim
and chlorpyriphos.The reduction in the sporulation intensity was observed at EC50 level as
compared to the required ideal cfu which is generally 4 - 8 x 108 The range of EC50 against
bavistin was 0.1-0.2 µg/ml and benomyl was (2.3-3.5µg/ml reducing the sporulation intensity to
(0.5 – 1.15at 106dilution). In the category medium risk pesticides range of sporulation intensity
was (2-10 at 106 ) and in low risk sporulation intensity was above 10x106 . The range of
sporulation intensity of all the isolates differed against the EC50 values, which clearly exhibits
the varied potential of the isolates in their sensitivity towards the pesticides.
1
Biologically active isolates TH3 and TH10 compatible with recommended doses of
thiram, captaf, iprodion, and mancozeb showed an increased overall growth/performance of
treated tomato, and cauliflower, in terms of plant vigor, root colonization and disease control,
indicates better performance of Trichoderma under solarized soils than non solarized soil.
Technology Vision 2020 project titled “On Farm Demonstration and Commercial
Cultivation of Trichoderma as Bio-Pesticide & Growth Promoter”.
Dissemination
of Trichoderma Technology in Farmers field against soil and foliar
diseases in crops grown in Jaipur Semi-arid Eastern Plain Zone and Kota districtsSouth-eastern
Humid Plain Zone of Rajasthan. Effective strains of Trichoderma harzianum, a potential
bioagents have been developed. This Trichoderma technology was needed to be disseminated in
the farmer’s field and therefore, a project entitled “On farm demonstration and commercial
production of Trichoderma as biopesticide and growth promoter” was formulated with the
support of the funding agency Technology Information Forecasting and Assessment Council
(TIFAC) - Department of Science and Technology (DST) for November, 2008 to March, 2012.
The major objectives of the project were to demonstrate low cost techniques for effective crop
protection through Trichoderma bioformulations (both powder form @ 4g/kg and liquid form @
4ml/L) through Integrated Pest Management (IPM) in selected villages of two different districts
of Rajasthan viz., Jaipur and Kota, and to develop replicable entrepreneurship model for
commercial application and production of Trichoderma bioformulation. With the collaboration
of NGOs viz., Society for Environmental and Development, Digod, Kota; Mahatma Gandhi
Gramin Vikas Evam Anusandhan Kendra, Samode; Global Green Tech, Chaksu; Centre for
Rural Development (CRD), Chaksu, Jaipur, the Trichoderma technology was demonstrated in a
total of twenty villages of Jaipur and Kota districts in more than 22 crops (Wheat, Barley,
Fenugreek, Brinjal, Chilli, Cauliflower, Mustard, Tomato, Potato, Pea, Onion, Okra, Garlic and
Coriander, Groundnut, Pearl millet, Cauliflower, Maize, Soya bean, Rice, Maize and Linseed) at
soil treatment, seedling, flowering and pre-harvesting stages which resulted in approx. 20%
increase in yield, approx. 62% reduction in disease and increase in net income of farmers.
Biological control of Groundnut root rot in Rajasthan:
Groundnut is an important oilseed crop predominantly grown in Rajasthan, India suffered from
55 to 85 percent root rot disease caused by multiple pathogen complex mainly Aspergillus niger,
Apergillus flavus, Sclerotium rolfsii, Thievaliopsis basicola, Rhizoctonia solani and Pythium
aphanidermatum perennating in soil and seed. Trichoderma harzianum (Th3) was used against
Groundnut varieties, GG-10, GG-20, M-13 and Local varieties in 2009 and 2010 and 2011 at
farmer’s fields in twelve different villages of Jaipur district. Field trials on soil, seed and foliage
treatment with powdered bioformulation (Th3 SD, SA) @ 4-5g per kg seed/soil followed by the
spray treatment of the liquid bioformulation (Th3 FS) @ 4-5 ml/l along with recommended IPM
practices were conducted. The crops under farmers practice were significantly low in yields with
the diagnostic blackening symptoms travelling from roots to stem affecting the vascular system
1
followed by shredding at root-stem internodes resulting in complete wilting and plant death
while in Th3 treated crop blackening reduced and the root vascular system was free of disease.
Maximum values of yield (39.17 Q/ha), R.C. Index (0.15), C.F.U. (38.5 x 106), and lowest root
rot incidence (14.03%) was recorded. Increase in annual income also encouraged farmers to use
the Trichoderma technology. Participatory approach and interaction between researcher and
farmers helped in quick adoption and dissemination of use of biocontrol agents for groundnut
growers in Rajasthan state, India.
Use of Trichoderma harzianum (Th3) as growth promoter in Wheat
Validation of the Trichoderma technology for growth promoting ability of Trichoderma
harzianum (Th3) was evaluated in the popular wheat (Triticum aestivum L.) variety Raj 3765 at
farmer’s field in Jaipur and Kota. Rhizospheric Competence Index along with its growth
promotion effect on rootlets, tillers, weight of grains and grain yield were evaluated by using it at
three stages of crop viz., seed, flowering and pre-harvesting @ 4g/kg and @ 4ml/L along with
soil treatment with a mixture of farm yard manure and formulation @ 50:1 before sowing.
Compared to the first year where the farmers were unaware of Trichoderma in 2008-09, a
significant increase in yield of wheat from 36.25 to 46.73Q/ha (29% in Jaipur) and from 36.88 to
50.12Q/ha (36% in Kota) has been observed after continuous application for three years (20082011) The total income and the benefit cost ratio of farmers increased both at Jaipur (Rs
56242/ha, 1:1.8) and Kota (Rs 60332/ha, 1:1.9).
Rhizosphere colonization and competence of Trichoderma in crops
Field performance of Trichoderma harzianum (Th3), was evaluated in terms of its rhizosphere
colonization and competence, survivability (root colonization behaviour) and non host specificity
in different crops including cereals (Rice, Wheat, Maize, Pearl millet, Barley), Legume
(Chickpea), oilseed crops (Groundnut, Soybean, Mustard, Linseed ), Vegetables (Cauliflower,
Brinjal, Okra, Pea, Potato, Tomato, Chilli, Garlic, Onion, Coriander, Fenugreek) , Ornamental
crops (Rose, Marigold) and fruit (Watermelon) of both Rabi and Kharif season. Field trials on
seed treatment with powdered bioformulation of Trichoderma harzianum (Pusa Th3) @ 4-5 g/kg
seed, followed by spray of liquid bioformulation (Pusa Th3) @ 4-5 ml/l were conducted. The
associations of Trichoderma harzianum (Th3) with rhizosphere were measured by periodic
observation of rhizosphere competence and survivability at three different stages of crops
(seedling, flowering and pre-harvesting stage), has been taken at 20 different locations (Villages)
of two districts of Rajasthan (viz., Jaipur and Kota). Populations of Trichoderma harzianum
(Th3) were isolated on Trichoderma Selective Medium (TSM), was not only found antagonistic
to pathogen but also showed colonizing behavior to rhizosphere. Survivability of T. harzianum
was found maximum with R.C. Index value: 0.31 at flowering stage and c.f.u. value: 5.16 x
107/gm at pre-harvesting stage, while it was minimum with R.C. Index value: 0.16 and c.f.u.:
0.72x 107/gm at seedling stage of most of the crops. Trichoderma harzianum (Th3) was detected
in large quantities with every crop tested when roots with adhering soil (rhizosphere soil) were
1
assayed. Different crop species and cultivars of Groundnut, Soybean, Garlic, Onion, Coriander,
Mustard, Wheat, Cauliflower and Rice varied in rhizosphere densities of Trichoderma
harzianum (Th3), but rhizosphere population densities were consistently higher than in untreated
rhizosphere soils. Th3 also found to play major role in plant growth promotion in different crops
of Rajasthan and control major crop diseases. This tremendous potential of this biocontrol agent
can be well utilized by integrating it with IPM or organic farming systems and also can work for
crop protection and growth promoter strategy.
All the farmers and about thirteen entrepreneurs were trained with the Trichoderma technology
by regular on-field training, entrepreneur training programmes and by conducting Kisan melas.
Finally this technology was also commercialized by transferring the technology to Sai Bio
Organics, Moga, Punjab (India) on 17th September, 2010 and made easily available to all.
Biological Control of Purple Blotch of Onion
Purple blotch caused by Alternaria porri leads to heavy yield loss in both bulb and seed crops of
Onion. Using Biological Control Agents (BCAs) is an important approach for successful disease
management and sustainable onion production. Development of a BCA based strategy was
attempted, after testing 17 isolates of fungal and six isolates of bacterial bioagents for their
bioefficacy against A. porri. Effective isolates (Th-3, Th-30, Tv-12, Tv-15, Pf-3 and Bs-2) were
selected and tested for volatile and non-volatile metabolites production and vigour induction
under in vitro. Different methods for use of the BCA’s viz., seed treatment, seedling dip and
three foliar sprays were evaluated on Onion bulb crop under glass house and field conditions.
Trichoderma harzianum isolate (Th-3) expressed high level of disease reduction and growth
promotion in susceptible Onion (cv. Pusa Red). Field experiments on seed crop also confirmed
the potential of Th-3 isolate as an effective BCA.
Effect of BCAs on onion seed crop was studied to validate the results obtained from the bulb
crop under glass house and field conditions. Methods of treatment used were, bulb treatment and
foliar spray of BCAs. Disease incidence in T. harzianum (Th-3) treated plots was less (27. 36%)
compared to other BCAs but higher than Mancozeb 75 EC (0.25%) treated plot (15.2%). The per
cent plant disease reduction by the BCAs tested was between 78.3 and 52.7. Plants treated with
Th-3 showed better performance in parameters like number of leaves, plant height and seed
weight per plant over the control. Seed yield with Th-3 (210.2 kg/acre) was also higher than that
of control (152.9 kg/acre). Isolate Th-3 not only suppressed infection and invasion but also
induced growth. It has favourably affected yield in the seed crop also. Th-3 however showed
lower disease reduction (61.0%) potential than Mancozeb (78.4%).
Effect of biocontrol agents on onion bulb crop was studied under glasshouse (2007-08) and field
condition (2008-09) and on seed crop under field trials (2009-10) found that disease reduction
was higher with the use of isolate Th-3. It is hence observed that T. harzianum (Th-3) is the
most effective strain compared to others such as Th-30, Tv-12, Tv-15, Pf-3 and Bs-1. In addition
to the suppression of disease the antagonistic treatment is seen to induce plant growth. The
present study shows that Th-3 increases the growth and yield of both bulb and seed crop.
1
Management of Sclerotinia sclerotiorum causing stalk rot of cauliflower by non-chemical
methods.
An integrated management package was developed by using plant extracts with Trichoderma
harzianum Aqueous extracts of ten plants belonging to different families were tested in vitro at
three different concentrations (5, 10 and 15%) against the pathogen in terms of radial growth,
myceliogenic, carpogenic and ascospore germination. In all the in vitro experiments with plant
extracts garlic bulb extract (GBE) recorded highest inhibition of radial growth, myceliogenic,
carpogenic and ascospore germination of the fungus. GBE was followed by Azadirachta indica
or Tagetes erecta in one experiment or the other. All the other plant extracts significantly
reduced the pathogen on the above parameters. GBE at 15% concentration completely inhibited
radial growth of the pathogen which is at par with bavistin (0.1%) treatment. Two fungal
biocontrol agents T. harzianum Th3 and A. niger AN27 were also tested against S. sclerotiorum
in terms of radial growth, mycelial and carpogenic germination. T. harzianum Th3 was found
more inhibitory than A. niger AN27 on above parameters. T. harzianum Th3 completely
inhibited carpogenic germination of the pathogen being at par with bavistin (0.1%) treatment.
Further, the two fungal biocontrol agents were tested in vitro for compatibility with GBE in
terms of radial growth, sporulation and spore germination parameters. Both the biocontrol agents
were inhibited significantly by GBE but T. harzianum Th3 was inhibited less than A. niger
AN27. On the basis of this compatibility study T. harzianum Th3 was chosen for integration with
GBE in vivo along with organic amendments. In the field experiment conducted during Rabi
2005-06 and 2006-07 with 14 treatments taking T. harzianum Th3, GBE and three organic
amendments viz., farm yard manure (FYM), mustard cake and vermicompost in different
combinations, the treatment of [FYM @ 50 t/ha + seed treatment with Th3 + seedling dip with
Th3 + GBE spray (15%)] recorded lowest disease index value of 9.5 among all non-chemical
treatments compared to 37.0 in control and highest seed yield of 370.0 kg/ha as compared to
214.2 kg/ha in control in the year 2005-06. This treatment was followed by [mustard cake @2.5
t/ha + seed treatment with Th3 + seedling dip with Th3 + GBE spray (15%)] and [vermicompost
+ seed treatment with Th3 + seedling dip with Th3 + GBE spray (15%)] recording disease index
value and seed yield of 11.2 and 356.9 t/ha and 12.0 and 343.0 t/ha respectively. The same trend
was followed in the year 2006-07 in terms of both disease index value and seed yield.
Molecular characterization of Trichoderma spp.:
The partial sequences of ITS region of Trichoderma isolates were deposited in the Gene
Bank with the following accession number viz., Th3EU 365835, Th10 EU382224,
Th30 EU443841, Th31 EU443842, ThAg EU443843, Tv2 EU443844,Tv4 EU443845,
Tv12 EU443846, Tv15 EU443847, Tv32 EU443848, TvChen EU443849, TvNir EU443850,
Hypocrea rufa EU443851. The dataset generated through morphological characters and
molecular markers (RAPD and ISSR) showed a comparable output grouping the isolates Tv4,
TvChen and TvNir in one cluster and all T. harzianum isolates in another cluster. It is obvious
1
from the present study that genetic diversity analysis had a positive correlation with the
antagonistic ability of Trichoderma isolates. Thus an integrated approach of morphological and
molecular markers can be employed to identify a superior strain of Trichoderma for its
commercial exploitation.
Isolation and Characterization of Important Biocontrol Genes (Endochitinase (ech42),
Xylanase and beta tubuline) of Trichoderma harzianum (Th3)
Endochitinase (ech42) gene which is involved in mycoparasitism, was isolated from
Trichoderma spp. taken from hot-arid soils of Rajasthan, cloned, sequenced and its expression
profiling was carried by reverse transcription-polymerase chain reaction (RT-PCR) technique.
The cloned DNA sequence was 1,476 base pairs. Gene encoding endochitinase was ligated in
pGEMT cloning vector. The plasmids were transformed in DH5α Escherichia coli competent
cells and clones were confirmed through sequencing and restriction analysis. Endochitinase gene
expression was then observed for different Trichoderma isolates viz., T. harzianum (T14 and
T12) and T. atroviride (T5). Among the three, higher expression of endochitinase was observed
in T14 and T12, whereas T5 showed lesser expression with respect to T14 and T12 strain. The
Trichoderma chitinase enzyme activity was monitored for all isolates under study. The highest
chitinase activity was observed in T14 and T11 viz., 17.21 (1 enzyme μg/ml) and 13.11 enzyme
μg/ml, respectively. Total protein was isolated from the T.harzianum isolate and induced ech42
protein was purified. The stability of the ech42 protein was checked at different pH and
Temperature.
A study was started to evaluate thirty-four Trichoderma spp such as T.harzianum, T.viride and
T.atroviride isolates on the basis of xylanase activity. Experiment was conducted by using Solid
State Fermentation (SSF) method using sugarcane bagasse acting as a nutrient carbon source for
Trichoderma. The highest Xylanase activity was observed in T. harzianum (T14 Untreated) and
(T34 Treated) viz., 51.47µg/ml and 43.6µg/ml, T. atroviride (T2 Untreated) and (T33 Treated)
34.42µg/ml and 19.67µg/ml, and T. viride (T7Untreated) and (T32Treated) 17.29µg/ml and
11.08 µg/ml, at 28°C with respect to other counterparts. It is worth considering that the
enzymatic activity started increasing with increase in pH highest activity at pH5 was achieved by
using phosphate buffer. The T. harzianum (T14) partial length copy of the genomic DNA
(400bp) was amplified and the expression of the cloned xylanase gene was analysed by RT-PCR.
Both genomic DNA and cDNA of the xylanase were obtained and analysed. Amino acid
sequence comparison with other xylanase exhibited 100% identities to xylanase from Hypocrea
lixii.
Carbendazim are group of benzimidazole fungicides used by the farmers in their agricultural
practices. β-tubulin gene has been identified as a primary key binding site for this group of
fungicides. An attempt was made using molecular genetic techniques to identify sites of
interaction of beta-tubulin receptors of biocompetent Th3 with benzimidizole anti-microtubule
1
agents. The isolate Trichoderma harzianum (Th3) was selected through rigorous screening of
isolates which confer resistance to the benzimidazole agent including benomyl and its active
breakdown product, carbendazim which became the centre of our interest. β-tubulin sequences
were obtained through cloning of wide range of collected competent isolates from different ecozones of the country. qPCR results showed higher expression in Th3 than other counterparts. An
increased demand for food and fiber has lead to the chemicalization of agriculture and we have
reached that stage where we are totally dependent on fertilizers and chemicals which in real
sense polluting our life and ecology by entering food-chains. Experiment was conducted under In
vitro conditions to determine the breakdown potential of biocompetent Th3 to mitigate the effect
of carbendazim, through HPLC analysis. The isolate was grown in mineral salt medium that
contained different concentrations of carbendazim ranging from 10ppm, 50ppm, 100ppm, 150
ppm and 200 ppm respectively. The metabolite detected through HPLC was benzimidazole after
2 days of shake incubation. There was a steep decrease in carbendazim content observed in all
ppm concentrations of carbendazim under test. Absence of carbendazim was also observed in
10ppm, 50 ppm and 100ppm concentrations after 3 days of treatment. It showed that Th3 strain
shows maximum breakdown percentage reaching upto 98% which is quite promising to consider
Th3 as a tool for soil bioremediation studies.
Development of bioformulation and delivery system of Pseudomonas fluorescens for the
management of Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) of rice
Bacterial leaf blight of rice caused by Xanthomonas oryzae pv. oryzae is the second most
important disease of rice after blast and most important bacterial disease in terms of economic
loss. The disease causes straw coloured strips either on one or both margins of leaves. It starts
from tip and goes downwards causing drying of leaf tips, inward rolling and twisting of infected
portion. Rhizosphere of crop plant harbor unique microflora. Many of the fluorescent
pseudomonads particularly Pseudomonas fluorescens have been shown to be effective root
colonizer and strong biocontrol agent. Delhi isolate of Pseudomonas fluorescens Plant Growth
Promoting Rhizobacteria (PGPR) has been isolated and characterized. It has been found
antagonistic to the most virulent isolate (Kaul isolate) of Xanthomonas oryzae pv. oryzae in
Northwestern plains of India. Commercial application of PGPR either to increase crop health or
to manage plant diseases depend on the development of commercial formulations with suitable
carriers that support the survival of bacteria for a considerable length of time. Therefore the
carriers used were talc, kaolinite, wheat bran, barley bran and soybean bran. Shelf life of talc
based bioformulation was found best which showed highest mean bacterial population (313.3 X
107 cfu/g) at 30 days of storage and there after declined to 38.3 X 107 cfu/g even after 150 days
of storage. In wheat based formulation the initial population of Pseudomonas fluorescens was
172.6-174.3 X 107 cfu/g. Surprisingly the mean population after 30 days of storage declined to 4
X 107 cfu/g and thereafter declined to zero population. Similar results were obtained in soybean
based bioformulation Shelf life study reveals that wheat and soybean bran do not support the
1
growth of bacterium. Survivability study of P. fluorescens in rhizosphere showed that the
population was found maximum at 45-60 Days after irrigation. It was 146 X104 cfu/g to 157 C
104 cfu/g in talc based bioformulation. The most effective delivery method was seed treatment
with talc based bioformulation which reduced disease intensity up to 93.3% and 87.5%in
glasshouse & microplot conditions respectively 21Days after inoculation. Root dip treatment
with talc based bioformulation treatment reduced disease intensity by 85.3% and 76.3% in glass
house and field study. Delivery method with irrigation was not found very effective in reducing
disease intensity.
With Division of Extension, IARI:
Associated in the activities of dissemination of IARI technologies in Udaipur district: 2008
onwards Participation in technology dissemination. Development
of Four villages as
“Lighthouse” (Model Villages) in National Capital Region of Delhi (from Kharif, 2011 to Rabi
2012-13). The villages covered were i) Badarpur Said, Faridabad, Haryana, ii) Dhani
Kumbhawas, Gurgaon, Haryana, iii) Partapur, Ghaziabad, Uttar Pradesh and iv) Pabeshra,
Sonepat, Haryana, member of Extension council and Production Unit associated wit Extension
Activity.
1
TRAINING COURSES /PROGRAMMES ORGANIZED
(Title)
Year
Duration
Use of Trichoderma on the farmers field at 2010
27th March
No. of
beneficiaries
>80
Jaipur
Use of Trichoderma on the farmers field at
Kota
Biopesticides development and its application
at Jaipur
Entrepreneur training programme on
Trichoderma biopesticides at IARI, New
Delhi
Use of Trichoderma in Disease Management
under Integrated disease management
program at Gramin Vigyan Kendra –Digodh,
Kota.
Plant growth enhancement and disease control
by Trichoderma harzianum in vegetable
seedlings grown under commercial conditions
at Samode and Dodhsar Village-Jaipur.
2010
28rd March
>80
2010
2nd and 3rd Nov.
>80
2010
27 Sept – 1 Oct.’
13
2011
15th October to
20th October
80
2011
21st October to
31st October
35 + 50
Training Programme on Use Of Trichoderma
Technology
2012
13-16
December,2012
10 farmers
ORGANIZATION OF KISAN MELAS, EXHIBITIONS
(Title and location) etc.
Year
Duration
“Jaivic Paddhati Dvara Fhasal Suraksha”,
at Dodhsar village, Jaipur district
31st
Oct.’
2009
2nd
Nov.’
2010
3rd
Nov.’
2010
1st Nov.’
2011
“Jaivic Paddhati Dvara Fhasal Suraksha”
at Kota District
“Jaivic Paddhati Dvara Fhasal Suraksha”
at Jaipur District
Jaivic Paddhati Dvara Fhasal Suraksha” at
Dodhsar village, Jaipur district
1
No. of
beneficiaries
1 day
>100 farmers
1 day
>100 farmers
1 day
>100 farmers
1 day
>100 farmers
BOOKS PUBLISHED
Books Authored:
1. Pratibha Sharma. 2012. Biocontrol of Plant Diseases ,ICAR, P-400( under publication )
2. Pratibha Sharma. 2007. Vegetables: Disease diagnosis and biomanagement. Ppxii+218.
Aavishkar Publications, Distributors, Jaipur, P-300
3. Sharma Pratibha, and S.P.S. Raghav. 2000. Disease Management of Ornamental
Crops. Division of Floriculture and Landscaping, I.A.R.I. New Delhi, P-100
Books Edited:
1. A.K. Misra, P. Chowdappa, P. Sharma and R. Khetarpal. 2012. Series no. 1: Diseases
of fruit crops in India. IPS and Today and Tomorrow publication, pp. 500.
2. T.S. Thind, R.K. Jain, Pratibha Sharma, S.M.Paul Khurana, Rashmi Aggarwal, R.K.
Sharma, Dinesh Singh, S.C. Dubey and A. Kumar. 2012 Plant Pathology Vision 2030.
Indian Phytopathological Society, Division of Plant Pathology, Indian Agricultural
Research Institute, New Delhi, India, pp.294.
3. Pratibha Sharma. 2012. Significant Post Graduate Students’ Research compilation at
Golden Jubilee Convocation, Post Graduate School, IARI, New Delhi, India, pp.42.
BOOK CHAPTERS/MANUALS:
1. Pratibha Sharma, Dinesh Singh and Rashmi Aggarwal. 2012-13. A Practical Manual
On Biocontrol of Plant Diseases. Post Graduate Manual. Indian Agricultural Research
Institute, New Delhi.
2. Pratibha Sharma. 2012. Principles and Practice of Biological Control in field.
Technology, Information, Forecasting and Assessment Council (TIFAC), New Delhi
funded Agricultural Developmental Programme, pp150.
3. Dinesh Singh,
Kalyan Mondal and Pratibha Sharma. 2011. A Practical manual on
Plant Bacteriology. Post Graduate Manual. Indian Agricultural Research Institute, New
Delhi. pp.100.
4. Pratibha Sharma, S. Deep and M. K. Saini. 2011 Biological
control
“Disease
Diagnosis, Evaluation and Management.” Technology, Information, Forecasting and
Assessment Council (TIFAC), New Delhi funded Agricultural Developmental
Programme. pp.60
1
5. Pratibha Sharma. 2011. Entrepreneurship model for The Trichoderma Technology.
Technology, Information, Forecasting and Assessment Council (TIFAC), New Delhi
funded Agricultural Developmental Programme.pp.50.
6. G.L.Sharma, P. Sharma and V.L. Majumdar. 2011. Cereal Cyst Nematode, Heterodera
avenae, A Menace causing Phytonematode in Cereals in Light Soil. In: Phyto-nematodes
in Crops: Their Identification, Treatment and Management (Eds: G.L.Sharma,). New
India Publication Agency, New Delhi (India). pp.1-8.
7. Chowdappa, P. and Sharma, Pratibha. 2011. Fungal foliar diseases of annual and
perennial crops: Challenging national food and nutritional security. In: Plant Pathology in
India: Vision 2030 (Eds. T.S. Thind et al.), Indian Phytopathological Society, Division
of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India, pp. 101104.
8. Sinha, P., Jain, R.K. and Sharma, Pratibha. 2011. Plant disease monitoring for
adaptation strategies under climate change scenario. In: Plant Pathology in India: Vision
2030 (Eds. T.S. Thind et al.). Indian Phytopathological Society, Division of Plant
Pathology, Indian Agricultural Research Institute, New Delhi, India, pp. 121-127.
9. Pratibha Sharma, Swati Deep and S. Gothandapani. 2010. Alternaria Leaf Blight of
Cruciferous Vegetables. Technical Bulletin, ORP on Leaf Spot Diseases Series 6, ICAR.
10. Pratibha Sharma, Monika Tripathi, Monica S. Henry and Amarnath Patel. 2010.
“Rogon Kee Pehchaan, Mulyankan Evam Niyantran”. ICN: H-87/ 2010 .
11. Pratibha Sharma. 2009. “Trichoderma Upcharan Kee Saral Vidhiyan”. TIFAC project(
in Hindi )
12. Pratibha Sharma et al. 2007 “A Practical manual on Diseases of Horticultural Crops.”
“A practical manual on biocontrol of plant pathogens.” IARI.
13. Pratibha Sharma. 2007. Vegetables: Disease diagnosis and biomanagement. Aavishkar
Publications, Distributors, Jaipur, Ppxii+218.
14. G. Kulsreshtha , M. Gopal, P. K. Malhotra, P. Sharma, I. Mukherjee, S. B. Singh, Ranbir
Singh, S. Chandra. 2005. Development of IPM Pesticide residue free IPM package for
Chillies NATP Production System Research PSR-41, Division of Agricultural Chemicals,
IARI, New Delhi-12.
15. G. Kulsreshtha, M. Gopal, P. K. Malhotra, P. Sharma, I. Mukherjee, S. B. Singh , Ranbir
Singh, S. Chandra. 2005. Development of IPM Pesticide residue free IPM package for
Cabbage NATP Production System Research PSR-41, Division of Agricultural
Chemicals, IARI, New Delhi-12.
16. G. Kulsreshtha , M. Gopal, P. K. Malhotra, P. Sharma, I. Mukherjee, S. B. Singh, Ranbir
Singh, S. Chandra. 2005. Development of IPM Pesticide residue free IPM package for
1
Tomato NATP Production System Research PSR-41, Division of Agricultural Chemicals,
IARI, New Delhi-12.
17. Pratibha Sharma; R. Aggarwal; J. Gopalakrishnan and D. V. Singh, 2004. Practical
training Manual on “Biocontrol Strategies for Management of Plant Pathogens”. W.e.f 16
Nov, to 6 Dec.2004 at Centre of Advanced Studies in Plant Pathology Centre of
Advanced Studies in Plant Pathology.
18. Pratibha Sharma; Prem Dureja, and Sain, S.K. 2002
“Shakiya Phasalon Mein
Jeyvic Vidhi Dwara Rog Niyantran” ICAR, NATP, IARI Publication on eve of KISSAN
SAMMAN DIVAS 21-23 Dec.2002. pp.16.
19. Pratibha Sharma and Bhattacharya, S. K. 2002. Plant Protection in Ornamental Crops
AICFRP TECH BULL. 19. AICFRP, pp200.
20. Das, D.K., Shivkumar, B.G, Garg, R.N., Mishra, S.K., Sharma, P. and Singh, R.P. 2002.
National Symposium on Agriculture in Changing Global Scenario organized by Indian
Society of Agricultural Sciences and Indian Council of Agricultural Research, New Delhi
from Feb. 21-23. Society of Annals of Agricultural Research, pp 1-477.
21. Sharma, Pratibha, D. Adalakha and M. Sindhu. 2001. Challenges and Opportunities of
Biological control in Plant Disease Management”. W.e.f 16 Oct. to 5 Nov. 2001 at Centre
of Advanced Studies in Plant Pathology (Sharma Pratibha and D. V. Singh)
22. Shukla Pratibha, B. Singh and N. K Dadlani, 1993. Diseases of Roses in India
Publication from Division of Floriculture and Landscaping. AICFRP.
LIST OF IMPORTANT PUBLICATIONS:
1. Prashant P Jhambulkar and Pratibha Sharma. (2013). Promotion of rice seedling growth
characteristics by development and use of bioformulation of Pseudomonas fluorescens.
Indian Journal of Agricultural Sciences. 83 (2): 136-142.
2. R.T.P.Pandian, P. Sharma, V.K.Singh, A.Singh, R.K. Ellur, A.K. Singh and U.D. Singh
(2012). Evaluation of Tetep derived blast resistant Basmati rice breeding lines for their
reaction to sheath blight disease. Indian Phytopathology 65(3): 233-237
3. Jambhulkar P.P. and Sharma P (2012). Comparative survivability of Pseudomonas
fluorescens RRb 11 in different carriers and rhizosphere. The Bioscan, 7(3): 507-511.
4. Pratibha Sharma, Amar Nath Patel, Mahesh Kumar Saini and Swati Deep (2012). Field
demonstration of Trichoderma harzianum as a plant growth promoter in wheat (Triticum
aestivum L).Journal of Agricultural Science, 4(8): 65-73.
5. Pratibha Sharma, Mahesh Kumar Sain, Swati Deep and Vignesh Kumar (2012).
“Biological Control of Groundnut root rot in Farmer’s field”. Journal of Agricultural
Science, 4(8): 48-59.
1
6. V. Prakasam and Pratibha Sharma (2012). Exploiting a potential strain of bio-agent
against Alternaria porri and increasing vigour index in onion. Indian Journal of Plant
Protection Sciences, 40 (3)( accepted for publication )
7. Desh Bandhu Ahuja, Usha Rani Ahuja, P. Srinivas, R.V. Singh, Meenakshi Malik,
Pratibha Sharma, O. M. Bambawale (2012). Development of Farmer-led Integrated
Management of Major Pests of Cauliflower Cultivated in Rainy Season in India. Journal
of Agricultural Science, 4: (2) 79-90.
8. Pratibha Sharma, Saravanan K, Ramesh R, Vignesh Kumar P, Dinesh Singh, Manika
Sharma, Monica S. Henry and Swati Deep (2012). Cloning and Semi-quantitative gene
expression of endochitinase (ech42) gene from Trichoderma spp. African Journal of
Biotechnology, 11(66): 12930-12938.
9. Pratibha Sharma, Swati Deep, Manika Sharma, Dinesh Singh Bhati (2012). Genetic
variation of Alternaria brassicae (Berk) Sacc causing dark leaf spot of cauliflower and
mustard in India.Journal of General Plant Pathology, DOI: 10.1007/s10327-012-0417-3.
10. Swati Deep and Pratibha Sharma (2012). Host age as predisposing factor in black leaf
spot of Cauliflower caused by Alternaria brassicae and Alternaria brassicicola. Indian
Phytopathology, 65(1):71-75.
11. Sharma Pratibha, A.N. Patel, S. Deep, M.K. Saini, P.P. Jambulkar, O. P. Gangwar and
V. Prakasham (2012). Field performance of Trichoderma harzianum (Th3) for
Rhizosphere competence and survivability in different agriculturally important Rabi
crops. e-planet, 9 (2): 8 - 13.
12. Prashant P Jhambulkar and Pratibha Sharma. (2013). Identification of suitable
bioformulation of Pseudomonas fluorescens for promotion of paddy seedling growth
characteristic and control of bacterial leaf blight. Indian Journal of Agricultural Sciences.
(Accepted publication).
13. Jambhulkar, P.P. and Pratibha Sharma (2011). Promotion of rice seedling growth
characteristics by development and use of bioformulation of Pseudomonas
fluorescens. Journal of ecofriendly agriculture, 7(2): 104-108.
14. Muthu Kumar A, Sharma P (2011) A study on corroboration between DNA markers
(RAPD, ISSR, ITS) and bio-control efficacy of Trichoderma species. Fungal Genom
Biol, 1:103. doi: 10. 4172/ fgb. 100010.
15. Pratibha Sharma, Vignesh Kumar P, Ramesh R, Saravanan K, Deep S, Sharma M,
Mahesh S and Dinesh S (2011). Biocontrol genes from Trichoderma species- A Review.
African Journal of Biotechnology, 10(86): 19898-19907.
16. Susanta Banik and Pratibha Sharma (2011). Plant pathology in the era of
nanotechnology. Indian Phytopathology, 64: (2) 120-127 (Won K.C. Mehta and
Manoranjan Mitra Award.)
17. Mousa Najafinia and Pratibha Sharma (2011). Development of specific PCR-based
marker for identification of pathogenic groups of Fusarium oxysporum f.sp. cucumerinum
in India. Journal of Gentetic engineering and biotechnology, 9: (1)29-34.
1
18. Mousa Najafinia and Pratibha Sharma (2011) Characterization of Indian isolates of
Fusarium oxysporum f. sp. cucumerinum using vegetative compatibility groups (VCGs)
and RAPD assay. Indian Phytopathology, 64: (1) 12-19.
19. Muthu Kumar A and Pratibha Sharma (2011) Molecular and morphological characters:
An appurtetenance for antagonism in Trichoderma spp. African Journal of
Biotechnology. African Journal of Biotechnology, 10 (22): 4532-4543.
20. Vishnu Vats, R. K. Upadhyaya and Pratibha Sharma (2010). Synthesis and antifungal
activity of 2-ketophenyl-3-substituted aryl-1-thiazolidin-4-ones. E-Jounal of Chemistry,
7(3):1040-1044.
21. Mousa Najafinia and Pratibha Sharma (2009) Cross pathogenecity among isolates of
Fusarium oxysporum causing wilt in cucumber & muskmelon. Indian Phytopathology. 62
(1). 9-13
22. Sulekh Chandra, Deepali Jain, Amit Kumar Sharma and Pratibha Sharma (2009)
Coordination Modes of a Schiff Base Pentadentate Derivative of 4-Aminoantipyrine with
Cobalt (II), Nickel (II) and Copper (II) Metal Ions: Synthesis, Spectroscopic and
Antimicrobial Studies. Molecules. 1-17.
23. Sain SK, Gour HN and Sharma Pratibha (2008). Pathogenic Variability and Antibiotic
Sensitivity in Xanthomonas campestris pv campestris isolates Causing Black rot of
Cauliflower. Journal of Mycology Plant Pathology, 509-604.
24. Sharma, Pratibha (2008) Effect of cropping system on rhizospheric competence of T.
harzianum and growth performance of important vegetable crops. Journal of Ecofriendly
Agriculture 3(2): 185-189.
25. S.K.Sain, H.N.Gour and Pratibha Sharma (2007). Evaluation of botanicals and PGPRs
against Xanthomonas campestris pv. campestris, an incitant of black rot of cauliflower.
Journal of Ecofriendly Agriculture, 2(2): 178-182.
26. Sharma, P (2007). Mass production techniques of Trichoderma, Kalisena and
Pseudomonas fluorescence, in Manual on Biomanagement strategy of Plant Pathogens,
17-20.
27. Sharma, Pratibha and Nidhi Sharma (2007). Hypovirulence as tool of biological
control. Journal of Ecofriendly Agriculture, 2 (1): 46-50.
28. Sharma, H.K., D. Prasad, Pankaj and Pratibha Sharma (2006). Bio-management of
Meliodogyne incognita infesting Okra. Ann.pl.Prot.Sci,14 (1) 191-193
29. Sharma, Pratibha, S.R.Sharma, S.K.Sain, and A. Dhandpani (2006). Integrated
management of major diseases of cauliflower (Brassica oleracea var.botrytis subvar
cauliflora). Indian Journal of Agricultural Sciences, 76(12):726-731
30. Pratibha Sharma and S.K. Sain (2005). Use of biotic agents and abiotic compound
against damping of cauliflower caused by Pythium aphanidermatum. Indian
Phytopahology, 58(4): 395-401.
1
31. Pratibha Sharma, L.N.Kadu and S.K. Sain (2005). Biological management of dieback
and fruit rot of chilli caused by Colletotrichum capsici (Syd.) Butler and Bisby. Indian
journal of plant protection, 33(2): 226-230.
32. Qais,K. Zewain, P.Bahadur and Pratibha Sharma (2005). Integrated disease
management of stalk rot of cauliflower. Indian Phytopathology, 58 (2): 167-173.
33. Sain, S.K., H.N. Gour and P. Sharma (2005). Biocontrol of Black Rot of Cauliflower by
Plant-Growth Promoting Rhizobacteria. J.Mycol.Pl.Pathol, 35 (1): 99-102.
34. Sharma Pratibha, Q Zewain, P. Bahadur and S. K. Sain (2005). Effect of soil
solarization on sclerotial viability of Sclerotinia sclertiorum (Lib.) de Bary of cauliflower
(Brassica oleracea L. var. botrytis subvar, cauliflower D. C.)
Indian Journal of
Agricultural Sciences, 75:90-94.
35. Arora, S; Sharma, Pratibha and Madhuban Gopal (2004). Compatibility of fungicide,
iprodione with Trichoderma harzianum. Ann. Pl. Protec. Sci,12: 222-223
36. Qais K. Zewain, P. Bhadur and Pratibha Sharma (2004). Effect of fungicides and neem
extract on mycelial growth and myceliogenic germination of Sclerotinia sclerotiotum.
Indian. Phytopahtology, 57(1): 101-103
37. Sharma Pratibha and P. Dureja (2004). Evaluation of T.harzianum
and T.viride
isolates at BCA Pathogen Crop Interface. J. Mycol. Pl.Pathol, 34 (1): 47-55
38. Sharma Pratibha, G.Kulshrestha, M. Gopal, and L.N. Kadu (2004). Integrated
Management of Chilli Die Back and Anthracnose in Delhi Region.
Indian
Phytopathology, 57 (4): 427-434
39. Sharma, P., S.R. Sharma and M. Sindhu (2004). A detached leaf technique for
evaluation of resistance in cabbage and cauliflower against three major pathogens. Indian
Phytopathology,57 (3): 315-318
40. Sharma, Pratibha and S.K. Sain (2004). Induction of systemic resistance in tomato and
cauliflower by Trichoderma species against stalk rot pathogen. (Sclerotinia
sclerotiorum). J.Biocontrol, 18(1):21-28.
41. Gawande, S. and Sharma, Pratibha (2003). Changes in host enzyme activity due to
induction of resistance against downy mildew in cauliflower. Annals. Agril. Res,
24(2):316-325.
42. Sharma, Pratibha and S.K. Sain (2003). Evaluation of commercial and laboratory
formulations of bioagents and plant nutrients against wilt of tomato and damping off of
cauliflower. Indian J.Pl.Pathol, 21:105-109.
43. Sharma, Pratibha and S.K.Sain (2003). Development of suitable techniques for
evaluating virulence and biocontrol activity of Trichoderma isolates. Indian J.Pl.Pathol,
21:16-21
44. Sharma, Pratibha, S.K.Sain and James S (2003). Compatibility Study of Trichoderma
isolates With Fungicides against Damping-off of Cauliflower and Tomato caused by
Pythium aphanidermatum. Pesticide Research Journal, 15(2): 133-138
1
45. Sharma, Pratibha and A.P, Singh (2002). Multiple diseases resistance in roses against
foliar and flower pathogens. Indian Phytopathology, 55 (2): 169-172
46. Sharma, Pratibha (2002). Use of bioagents with pesticides in plant disease
management. New Agriculturist, 13(1): 54-60.
47. Sharma, Pratibha (2002). Induction of systemic resistance in cauliflower to downy
mildew by exogenous application of plant activator. Annals of Plant Protection, 10:199203.
48. J. Jain and P. Sharma (2001). Fusarium wilt resistance in Eight identified Multiple
disease resistant genotypes of Cucumis melo L. Cucurbits Genetics Cooperative Report,
24 : 26-28
49. Sharma, Pratibha and A.P, Singh (2001). Evaluation of rose cultivars against die back.
Indian Phytopathology, 54 (3): 388-89
50. Sharma, Pratibha, L. Singh and D. Adlakha (2001). Antagonistic potential of
Trichoderma and Aspergillus species on Sclerotinia sclerotiorum (Lib.) de Bary causing
rots in cabbage and cauliflower. Pesticides Information, 2:41-44.
51. Bhattacharya, A. and Shukla, P (2000). Changes in some phenol related enzymes in field
pea leaves infected with powdery mildew under rain-fed and irrigated conditions. Indian
J. of Agricultural Research, 34 (3): 147-151
52. Sharma Pratibha (2000). An Integrated approach for the management of Carnation wilt
caused by Fusarium oxysporum f. sp. dianthi (Pril. And Del.) Snyd. And Hans 2001. New
Botanist, 27:143-150.
53. Sharma, Pratibha (2000). Varietal evaluation of roses against black spot. Ann. Pl.
Protect. Sci, 8(2): 173-175.
54. Singh, A. P., B. Singh and P. Sharma (1999). A study on multistoried budding in roses
The Indian Rose Annual XVI, 86-89.
55. Singh, A. P., B. Singh and P. Sharma. (1999). Performance of some new IARI roses.
The Indian Rose Manual XVI, 44 -47.
56. Bhattacharya, A. and Shukla, P (1998). Nitrogen assimilation related enzyme activities
in Pisum sativum L. Indian Journal of Agricultural Research, 32 : 176-180
57. Bhattacharya, A. and Shukla, P (1996). Effect of Erysiphe polygoni DC infection on
activities of some hydrolytic enzymes and their metabolites in Pisum sativum L. Legume
Research, 19: 133-137.
58. Amin, K. S; Reddy, M. V; Nene, Y. L; Raju, T. N; Pratibha Shukla; Zote, K. K;
Arjunan, G; Bendre, N. J; Rathi, Y. P. S; Sinha, B. K; Gupta, R. P; Anilkumar, T. B;
Chauhan, V. B; Bidari, V. B; Gurdeep, Singh; Jha, D. K; Kausalya, Ganghadharan;
Singh, G. (1993). Multi-location evaluation of pigeonpea (Cajanus cajan) for broadbased resistance to sterility-mosaic disease in India. Indian Journal of Agricultural
Sciences, 63: 9, 542-546.
1
59. Shukla, P. and Chowdhary, P.N (1993). A new wilt disease of Roses caused by
Fusarium solani (Mart) Sacc. Advances in Horticulture & Forestry, 3: 172-173.
60. Venkataraman K. T, Singh B., Shukla P. and Raghava S. P. S (1993). Relative efficacy
of different fungicides for the control of powdery mildew in roses. South Indian
Horticulture, 41 (3): 184- 185.
61. Shukla Pratibha and A Bhattacharya (1992). Changes in activities of primary hydrolytic
and relative water content in Pisum sativum L Pre and Post infection of Erysiphe
polygoni DC. Legume Research, 15 (4): 153-160.
62. Shukla Pratibha and A. N. Asthana (1991). Sources of resistance in sterility mosaic
disease in pigeon pea. Agriculture Science Digest, 11(1): 51-55.
63. Shukla, P. and Chowdhary, P.N (1991). Fungi associated with Die Back of Roses. J.
Mycol. and Pl. Pathol, 21: 213-214.
64. Shukla, Pratibha and K. S. Amin (1991). Integrated disease management of powdery
mildew of field peas (Pisum sativum L.) caused by Erysiphe polygoni D. C. Legume
Research, 14: (2) 59-63.
65. Shukla, Pratibha, A. Bhattacharya and R.S. Shukla (1991). Effect of powdery mildew
infection on the metabolism of field peas (Pisum sativum) leaves II Changes in enzymes
related to nitrogen assimilation. New Botanist, (18) ¾: 80-85.
66. Shukla, Pratibha, A. Bhattacharya and R. S. Shukla (1990). Effect of environmental
parameters on the infection severity of Pisum sativum. New Botanist, 17 (3/4) 283-291.
67. Shukla, Pratibha, A. Bhattacharya and R. S. Shukla (1989). Changes in free amino acid
content due to powdery mildew of peas. Indian Phytopathology, 42(1): 297.
68. Shukla, Pratibha (1989). Note on effect of age of the host on development of powdery
mildew of peas. New Botanist, 16 (1-4): 93-95.
69. Shukla, Pratibha and S. S. Lal (1988). Effect of combined application of fungicides and
insecticides on the powdery mildew and pod borer of pea. Pesticides, 3:5-7.
70. Mishra, S.P., Shukla, Pratibha and S.P. Doshi (1984). Heterosis and combining ability
in pea. Agric.Sci. Digest, 4(3):126-128.
71. Mishra, S.P. and Shukla, Pratibha (1984). Inheritance of powdery mildew resistance in
pea. Z. Pflanzenzuchtg, 93:215-217.
72. Shukla, P (1984). Screening of lentil germplasm against Uromyces fabae. Indian J.
Mycol. Pl. Pathol, 14(1):89-90.
73. Shukla, Pratibha and S.P. Mishra (1984). Assessment of losses in yield due to powdery
mildew of pea. Indian J. Pl. Pathol, 14 (2): 197-199.
ABSTRACTS
1. Manika Sharma, Swati Deep, Dinesh Singh, D.V. Singh, P.Chowdappa and Pratibha
Sharma. 2012 Unravelling the link between transcript profiling and changing metabolism
of CYP83B1 during infection of Alternaria brassicae and Alternaria brassiciciola in
Cauliflower. National symposium on Blending Conventional and Modern Plant
1
2.
3.
4.
5.
6.
7.
8.
9.
Pathology for Sustainable Agriculture, w.e.f. 4-6 December, 2012 at IIHR, Bangalore,
pp-15
P.Chowdappa, K.R.Hema and Pratibha Sharma. 2012. Phenotypic analysis of
Alternaria solani isolates causing early blight of tomato. National symposium on
Blending Conventional and Modern Plant Pathology for Sustainable Agriculture, w.e.f. 46 December, 2012 at IIHR, Bangalore, pp-16.
P.Chowdappa, M.Jyothi Lakshmi and Pratibha Sharma. 2012. Protein profiling of plant
pathogenic Alternaria species. National symposium on Blending Conventional and
Modern Plant Pathology for Sustainable Agriculture, w.e.f. 4-6 December, 2012 at IIHR,
Bangalore, pp-16.
P.Chowdappa, B. Reddi Bhargavi and Pratibha Sharma. 2012. A comparative analysis
of the Alternaria brassicciola (Schwein.) Wiltsh and A.brassiae (Berk) associated with
cabbage and cauliflower. National symposium on Blending Conventional and Modern
Plant Pathology for Sustainable Agriculture, w.e.f. 4-6 December, 2012 at IIHR,
Bangalore, pp-17.
K.Saravanan, Manika Sharma, Pratibha Sharma. 2012. Evaluation and characterization
of Xylanase from competent Trichoderma spp. National symposium on Blending
Conventional and Modern Plant Pathology for Sustainable Agriculture, w.e.f. 4-6
December, 2012 at IIHR, Bangalore, pp-20.
Pratibha Sharma. 2012. Field application of Trichoderma technology in semi-arid
eastern plains and Humid South-Eastern Plains of Rajasthan state of India. Trichoderma
and Gliocladium 2012 workshop entitled “TG2012 Innovations and Applications” at
Commerce Building C1, C2 and Foyer, Lincoln University, New Zealand w.e.f. 27th to
30th August, 2012, pp-57.
Pratibha Sharma, M. Saini, A. Patel, S. Deep and D. S. Bhati. 2012. Rhizosphere
competence and survivability of Trichoderma harzianum (Th3) in the crops grown in
semi-arid eastern plains of Rajasthan state of India. Trichoderma and Gliocladium 2012
workshop entitled “TG2012 Innovations and Applications” at Commerce Building C1,C2
and Foyer, Lincoln University, New Zealand w.e.f. 27th to 30th August, 2012, pp-112.
Pratibha Sharma, U.S.Singh, Najam Waris Zaidi, P. Vignesh Kumar, K.Saravanan, D.
V. Singh and Manika Sharma. 2012. Biological and Molecular investigation of a
potential strain of Trichoderma harzianum against carbendazim. Trichoderma and
Gliocladium 2012 workshop entitled “TG2012 Innovations and Applications” at
Commerce Building C1, C2 and Foyer, Lincoln University, New Zealand w.e.f. 27th to
30th August, 2012, pp-37.
Pratibha Sharma, Manika Sharma, Swati Deep, Dinesh Singh. 2012. Effect of
different concentrations of Indole acetic acid (IAA) on growth pattern of Alternaria
brassicae and Alternaria brassicicola and expression profiling of genetic element
CYP83B1 corresponding to different concentrations. Perspectives in Phyllosphere
Biology w.e.f. 15 to 17 February 2012, Noida, India.
1
10. Pratibha Sharma, Swati Deep, Manika Sharma, Dinesh Singh. 2011. PCR based assay
for detecting pathogenic Alternaria brassicicola in Crucifers. National Symposium on
Biology of Infection, Immunity and Disease Control in Pathogen-Plant Interactions and
64th Annual Meeting of Indian Phytopathological Society w.e.f. December 2-4, 2011
(Won Best Poster Award). Indian Phytopathological Society, New Delhi-12 and
Department of Plant Sciences, School of Life Sciences, University of Hyderabad,
Hyderabad, Andhra Pradesh. p.163.
11. Pratibha Sharma, R.Ramesh, K.Saravanan and Swati Deep. 2011. Characterization of
β-tubulin gene from carbendazim resistant Trichoderma harzianum (Th3). International
Conference on Biopesticides 6, 11 – 16 December 2011, Chiangmai, Thailand. p.1.
12. Saini M.K. and Pratibha Sharma. 2011. “Field performance of Trichoderma harzianum
(Th3) for Rhizosphere competence and survivability in different crops.” National
Symposium on Biology of Infection, Immunity and Disease Control in Pathogen-Plant
Interactions and 64th Annual Meeting of Indian Phytopathological Society w.e.f.
December 2-4, 2011. Indian Phytopathological Society, New Delhi-12 and Department of
Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Andhra
Pradesh. p.48.
13. Sharma, Pratibha, Saravanan K, Ramesh R, Sharma M and Vignesh Kumar P. 2011.
Molecular characterization and sequence analysis of endochitinase gene from
Trichoderma harzianum. International Conference on Biopesticides 6, 11 – 16 December
2011, Chiangmai, Thailand.
14. Pratibha Sharma, A.N. Patel, S. Deep, M.K. Saini, P.P. Jambulkar, O. P. Gangwar and
V. Prakasham 2011 Field performance of Trichoderma harzianum (Th3) for
Rhizosphere competence and survivability in different agriculturally important crops.
National Symposium on “Sustaining agricultural productivity and food security under
changing climate”. During coming 11-12 November 2011., IRC Village, Bhubaneswar
741015, Odisha.
15. Pratibha Sharma, M. Tripathi, AmarNath Patel, and Swati Deep. 2011. Biological
control of root rots of groundnut in Rajasthan, India. In proceedings of American
Phytopathological Society (APS) and International Association for the Plant Protection
Sciences (IAPPS) Joint Meeting, 2011, 6-10th August, Phytopathology. Honolulu,
Hawaii, United States of America. p. S164.
16. Pratibha Sharma, S. Deep, S. Gothandapani, M. Sharma, P. Kalia and P. Chowdappa.
2011. Pathogenic and genetic diversity in Alternaria brassicae and Alternaria
brassicicola causing black leaf spot of cauliflower in India. In proceedings of American
Phytopathological Society (APS) and International Association for the Plant Protection
Sciences (IAPPS) Joint Meeting, 2011, 6-10th August, Phytopathology. Honolulu,
Hawaii, United States of America. p. S164.
17. Pratibha Sharma, S. Gothandapani, Amarnath Patel.2010. Rhizosphere competence and
survivability of Trichoderma harzianum (Th3) in Rajasthan, India. In proceedings of
1
National Symposium on Perspective in the Plant Health Management, 14-16th December,
B.A. College of agriculture, Anand Agricultural University, Anand, Gujrat.
p.44.
18. Pratibha Sharma, Swati Deep, S. Gothandapani. 2010. Biocontrol of black leaf spot
disease of Cauliflower by using Pesticidal resistant strain of Trichoderma harzianum
(Th3). In proceedings of National Symposium on Molecular Approaches for
Management of Fungal Diseases of Crop Plants. 27-30th December, Bangalore, India.
p.143.
19. Pratibha Sharma; M. Henry; G. Sellamuthu and V. Gupta. 2010. Morphological,
biochemical and molecular characterization of highly efficient Indian Trichoderma
isolates. In proceedings of National conference: Unravelling multiple facets of biological
control in national purview: Trends, Challenges and Societal Implications & Indian
Phytopathological Society zonal meeting (Mid-Eastern zone); 26-27th October, Institute
of Agricultural Sciences, Banaras Hindu University, Varanasi, p.154.
20. S. Gothandapani, Swati Deep, and
Pratibha Sharma. 2010. Cross infectivity of
Alternaria brassicae and Alternaria brassicicola in Crucifers. In proceedings of National
Symposium on Molecular Approaches for Management of Fungal Diseases of Crop
Plants. 27-30th December, Bangalore, India. p.65.
21. S. Gothandapani, Swati Deep, and
Pratibha Sharma. 2010. Cross infectivity of
Alternaria brassicae and Alternaria brassicicola in Cauliflower and Mustard. In
proceedings of Fourth Indian Horticulture Congress-2010, 18-21st November, New
Delhi, India. pp.153-154
22. Swati Deep, S. Gothandapani, P. Chowdappa and Pratibha Sharma. 2010.
Morphological, Pathogenic and Molecular variability amongst Alternaria brassicae and
Alternaria brassicicola in India. In proceedings of National Symposium on Molecular
Approaches for Management of Fungal Diseases of Crop Plants. 27-30th December,
Bangalore, India. p.64.
23. Swati Deep, Sushil Kumar, S. Gothandapani, Pritam Kalia, Pratibha Sharma. 2010.
Screening of Cauliflower germplasm lines against Alternaria black leaf spot. In
proceedings of Fourth Indian Horticulture Congress-2010, 18-21st November, New
Delhi, India. p.150.
24. Sharma, Pratibha. 2004. Development of bioformulation from improved strains of
Trichoderma for vegetable crops. In Proceeding of 26th Annual Conference & National
Symposium of ISMPP on Advance in Fungal Diversity and Host-Pathogen Interaction, 79th October, Goa University, Goa. pp. 163-172
25. Sharma, Pratibha. 2004. Integrated use of abiotic and biotic elicitors against downy
mildew suppression and enzymatic activity in cauliflower in Microbial Diversity:
Opportunities and Challenges (Ed.) S.P.Gautam et al. Shree Publishers & Distributers,
New Delhi-110002. pp. 279-286.
26. Sharma, Pratibha. 2004. Effect of fungicides on fungal antagonists of Sclerotinia rot of
cauliflower and selection of pesticide resistant biotypes. In Eds. P. Dureja, D. B. Saxena,
1
J. Kumar, Madhuban Gopal, S. B. Singh and R. J. Tanwar Society of Pesticide Science,
IARI, India. pp. 186-191
27. Sharma, Pratibha; A. Kaur and S.K.Sain. 2004. Variability in Sclerotium rolfsii and
differential response against Trichoderma harzianum and Trichoderma viride.
In
Proceeding of 26th Annual Conference & National Symposium of ISMPP on Advance in
Fungal Diversity and Host-Pathogen Interaction. 7-9th October, Goa University, Goa.
pp. 173-182.
28. Sharma, Pratibha; A. Kaur and S.K.Sain. 2004. Variability in Sclerotium rolfsii and
differential response against Trichoderma harzianum and Trichoderma viride. In
Proceeding of 26th Annual Conference & National Symposium of ISMPP on Advance in
Fungal Diversity and Host-Pathogen Interaction. 7-9th October, Goa University, Goa,
(Accepted for publication), pp. 42-43
29. Sharma, Pratibha. 2004. Some important techniques used for biocontrol against plant
pathogens, In Training Manual: NATP sponsored short-term training course on
“Entrepreneurship development in mass production and use of biological control agents
in agriculture systems”. Pp85-9.
30. Sharma, Pratibha; A. Kaur, S.K. Sain and S.Banik. 2004. Biological control of sclerotia
forming pathogens in vegetables: In Training Manual: Biocontrol strategies for
management of plant pathogens, pp-47-55.
31. Sharma, Pratibha; S.K. Sain, A.Kaur and A.M. Kumar. 2004. Evaluation of ecological
parameters of bioagents. In Training Manual: Biocontrol strategies for management of
plant pathogens, pp-16-20.
32. Sharma, Pratibha; S.K. Sain and A.Kaur. 2004. Evaluation of fungal bioagents by
improved methods against plant pathogens. In Training Manual: Biocontrol strategies for
management of plant pathogens, pp-1-7.
33. Sharma, Pratibha and S.K.Sain. 2004. Ecological and biochemical changes in
Trichoderma harzianum due to fungicides. In NBAIM-CAB International, UK Join
Workshop on Isolation, Preservation and conservation of Agriculturally important
microorganisms and use of potential molecular tools for their identification at NBAIM,
New Delhi,16-17 March, p55.
34. Sharma, Pratibha and S.K.Sain. 2004. Variability in Trichoderma harzianum and
T.viride isolates. In NBAIM-CAB International, UK Join Workshop on Isolation,
Preservation and conservation of Agriculturally important microorganisms and use of
potential molecular tools for their identification at NBAIM, New Delhi,16-17 March,
pp55.
35. Sharma, Pratibha and S.K.Sain. 2004. Development of suitable techniques for
evaluating Trichoderma isolates. In National Symposium on Crop Surveillance: Disease
Forecasting and Management at IARI, New Delhi Feb.19-21. Pp88.
36. Sharma, Pratibha and S.K. Sain. 2003. Use of Potential isolates of Trichoderma species
in Developing IPM Module for Vegetable Crops. Presented in National Symposium IPM
1
in Horticultural Crops for Protection of Environment & Biodiversity (Feb10-12, 2003) at
Zonal Agricultural Research Station, Chandangaon, Chhindawara (M.P.).
37. Sharma, Pratibha and S.K. Sain. 2003. Evaluation of Trichoderma isolates for pesticide
tolerance and their effect against damping off of tomato. 90th Session, Indian Science
Congress. Bangalore, 3-7 January ‘2003’.
38. Pratibha Sharma. 2003. Use of biocontrol agents in sustainable vegetable production,
In Proceedings of International Seminar on Downsizing Technology for Rural
Development. (Eds) S.Khuntia, A.Parida and Vibuti N Misra. Bhubaneswer, Orissa. Vol.I
pp. 193-202.
39. Gopal, M., Mukherjee, I., Singh, S.B., Sharma, P., Chander, S., Pant, S., Sharma ,R.K.,
Kadu, L.N.,Malhotra,P.K. and Kulshrestha, G. 2003. Effect of schedule based application
of pesticides on yield of tomato (Lycopescicum esculantum Mill). In Proceeding of
National Symposium on Frontier Avenues of Entomological Research, 5-7 Nov.2003 at
Division of Entomology, IARI, New Delhi.
40. Sharma, Pratibha and S. K. Sain. 2003. Use of Potential isolates of Trichoderma
species in Developing IPM Module for Vegetable Crops. National Symposium IPM in
Horticultural Crops for Protection of Environment & Biodiversity (Feb10-12, 2003) at
Zonal Agricultural Research Station, Chandangaon, Chhindawara (M.P.).
41. Sharma, Pratibha et. al. 2003.Integrated management of chilli die back and anthracnose
in Delhi region. Won the best paper award National Symposium IPM in Horticultural
Crops for Protection of Environment & Biodiversity (Feb10-12, 2003) at Zonal
Agricultural Research Station, Chandangaon, Chhindawara (M.P.) .
42. Sharma, Pratibha and S. K. Sain. 2003. Evaluation of Trichoderma isolates for
pesticide tolerance and their effect against damping off of tomato. 90th Session, Indian
Science Congress. Banglore, 3-7 January ‘2003’.
43. Sharma, Pratibha., S.R. Sharma and M. Sindhu, 2003. Evaluation of screening
techniques against Sclerotinia sclerotiorum of cabbage and cauliflower. 90th Session,
Indian Science Congress. Banglore, 3-7 January ‘2003’.
44. Mukherjee I., S.B. Singh, M. Gopal, T.K. Das, P. Sharma, S. Chander, S. Pant, D. Rao,
M. Kumar and G. Kulshrestha. 2003. Pesticide Residues in Cabbage Crop: Evaluation of
Chemical Module for IPM, Proc. 90th Indian Science Congress Part III (Advanced
Abstracts). (26) P27-28
45. S.B. Singh, I. Mukherjee, M. Gopal, T.K. Das, P. Sharma, S. Chander, S. Pant, D. Rao,
M. Kumar and G.Kulshrestha. 2003. Pesticide Residues in Chill Crop: Evaluation of
Chemicals module for IPM, XXXVth Annual Convention of Indian Society of
Agricultural Chemists Department of Agril. Chemistry and Soil Science, B.A. Collage of
Agriculture, GAU, Anand.
46. Pratibha Sharma, G.Kulshrestha, M. Gopal, S. Chander, I. Mukherjee, S.B. Singh, S.
Pant, L.N. Kadu and V. Singh. 2003. “Integrated Management of Chilli Die Back and
Anthracnose in Delhi Region” “National symposium on Integrated Pest Management In
1
Horticultural Crops for Protection of Environment and Biodiversity”, 10-12 Feb., 2003,
organized by-Zonal Agricultural Research Station JNKVV, Chandangaon, Chindwara.
480001 M.P.
47. Sharma, Pratibha. 2002. Disease management strategies of green house crops in Crop
Pest and Disease Management Challenges for the Millennium. Eds. (D. Prasad and S. N.
Puri Jyoti Publishers , New Delhi, pp. 211-220.
48. M. Gopal, S. Chander, D. Rao, G. Kulshrestha, P.K. Malhotra, P. Sharma, I. Mukherjee,
S.B. Singh, R.Singh, S. Pant M. Kumar. 2002. Developing an Integrated pest
Management package for cabbage: Insect control and yield enhancement, Proc. Of
Second International Agronomy Congress on Balancing Food and Environmental
Security – A continuing Challenge organized by Indian Soc. Agronomy, ICAR and
NAAS held at New Delhi from November 26th to 30th, 2002.
49. Sharma, Pratibha. 2002. Integrated disease management of rabi crops. Rabi CampaignOrientation Programme 2002-2003 Mitigating delayed monsoon Rabi meeting of ICARState at Bhopal 22Oct.
50. Singh, D. V. and Pratibha Sharma. 2002. Integrated disease management in floricultural
crops in Commercial Floriculture: Avenues and Scope in North East India 24-31 Oct.
pp224-232.
51. Sharma, Pratibha. 2002. Use of improved Trichoderma harzianum isolates against
major soil borne diseases of vegetable crops.
In 2nd International Agronomy
Congress on Balancing Food and Environmental Security A Continuing Challenge, New
Delhi, Nov. 26-30, 2002 Extended summaries, Vol. 2: pp 1014-1016.
52. Sharma, Pratibha. 2002. Integrated disease management: The usable packages for rice
and vegetable crops Oct 19, NCIPM.
53. Sharma, Pratibha. 2002. Use of bioagents with pesticides in plant disease management
4th Indian Agricultural Scientists and Farmer’s Congress, 16-17 February at C.C.S.
University Meerut. Pp 88-89
54. Sharma, Pratibha. 2002. Use of ecologically competent pesticide resistant bioagents in
Integrated Pest Management. National Symposium on Agriculture in Changing Global
Scenario at I.A.R.I., New Delhi by Indian Society of Agricultural Sciences, and ICAR,
21-23 Feb. pp.389-390
55. Sharma, Pratibha. 2002. Selection for benomyl resistance in T. harzianum used against
soil borne pathogens of cauliflower. Asian Congress of Mycology and Plant Pathology
Session. 9 :195-196
56. Sharma, Pratibha; Singh, Ram and Sharma, S.R. 2002. Multiple Disease Resistance in
cabbage against major pathogens. 54th Annual Meeting and National Symposium on
Crop Protection and WTO-An Indian Perspective held at C.P.C.R.I., Kasaragod from
Jan., 22-25. Pp 74-75.
57. Aewain, Qais. K.; Sharma, Pratibha and Bahadur, P. 2002.
The
role
of
temperature in sclerotial survival of Sclerotinia sclerotiorum. 54th Annual Meeting and
1
National Symposium on Crop Protection and WTO-An Indian Perspective held at
C.P.C.R.I., Kasaragod from Jan., 22-25. P 42
58. Sharma, Pratibha. 2002. Biological Management of Vegetable Nursery Diseases.
National Symposium on Agriculture in Changing Global Scenario held at I.A.R.I., New
Delhi From Feb., 21-23.
p.407.
59. Sharma, Pratibha. 2002. Production of biocontrol products at rural women site. In
proceedings on the National Workshop on Role of biotechnology in Women Upliftment
in the New Millenium, 24th and 25th January, 2002, Agra (U.P.), pp. 41-49.
60. Sharma, Pratibha. 2002. Potential use of Fungal Antagonists against Phomopsis Blight
of Brinjal (Solanum melongena L.). Proceedings of the 89th session of the Indian
Science Congress held in Lucknow from Jan.3-7. Pp 1-3.
61. Sharma, P.; P. N. Chowdhary, D. Adalakha and M. Sindhu. 2001. Multiplication of
bioagents and seed treatment techniques Manual on Challenges and Opportunities of
biological control in Plant Disease Management. (Eds. Pratibha Sharma and D. V.
Singh), pp65-70.
62. Sharma, P. M. Sindhu and D. Adalakha. 2001. Rhizosphere and root colonization
competence assay. Practical Manual on Challenges and Opportunities of biological
control in Plant Disease Management. (Eds. Pratibha Sharma and D. V. Singh), pp2325.
63. Sharma, P.; D. Adalakha, and M. Sindhu. 2001. Evaluation of commercial formulations
for microbial control of soil borne pathogens. Practical Manual on Challenges and
Opportunities of biological control in Plant Disease Management. (Eds. Pratibha
Sharma and D. V. Singh), pp9-11.
64. Sharma, P.; D. Adalakha, and M. Sindhu. 2001. Basic techniques of isolation and testing
of bioagents. Practical Manual on Challenges and Opportunities of biological control in
Plant Disease Management. (Eds. Pratibha Sharma and D. V. Singh), pp1-8.
65. Arora, Sumita; Gopal, Madhuban and Sharma, Pratibha. 2001. Bioefficacy and
Residues of Iprodione for Control of Alternaria brassicicola and Sclerotinia
sclerotiorum. National Symposium on Plant Protection Strategies for Sustainable
Agriculture held in I.A.R.I., New Delhi from Oct 12-13. Pp 159-160.
66. Prasad, D. and Sharma, Pratibha. 2001. Effect of Trichoderma harzianum on the
penetration of Meloidogyne incognita Race-I on Cowpea. National Symposium on
Plant Protection Strategies for Sustainable Agriculture held in I.A.R.I., New Delhi from
Oct 12-13. Pp 150-151.
67. Sharma, Pratibha and D. Adlakha. 2001. Trichoderma ki Rasayanik Avrodhi
Prajatiyaan. Souvenir of National Symposium on Ancient Indian Science, Engineering &
Technology interfaced with the modern Knowledge, by Swadeshi Science Movement of
India 14-15 Dec. pp 106-107.
68. Sharma, Pratibha. 2001. Effect of fungicides on fungal antagonists of Sclerotinia
sclerotiorum rot of cauliflower and selection of pesticide resistant biotypes. Extended
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abstracts of International Conference on Pesticides, Environment and food security
organized by Society of Pesticide Science, India 19-22 nov.2001, pp189-190.
69. Sharma, Pratibha, L. Singh and P.N Chowdhry, 2001. Antagonistic potential of
Trichoderma and Aspergillus species on Sclerotinia sclerotiorum (Lib.) de Bary causing
rots in cabbage and cauliflower. Proceedings of the Eighty Eighth Session of The Indian
Science Congress New Delhi 2001 (Agricultural Sciences), pp105-106.
70. P. N. Chowdhary and Pratibha Sharma. 2000. Differentiation in genera of
Erysiphaceae. In Training Programme on Identification of Plant Pathogenic and
Biocontrol fungi (14 Sept. to 13 Oct.) in Technical Manual in Centre of Advanced
Studies in Plant Pathology. pp. 130-137.
71. Sharma, P. P. N. Chowdhary and Leena Singh. 2000. Differentiation in genera of
Erysiphaceae. In Training Programme on Identification of Plant Pathogenic and
Biocontrol fungi (14 Sept. to 13 Oct.) in Technical Manual Centre of Advanced Studies
in Plant Pathology, pp.101-106.
72. Sharma, Pratibha, P. N. Chowdhary and Leena Singh. 2000. Identification of Alternaria
species. In Training Programme on Identification of Plant Pathogenic and Biocontrol
fungi (14 Sept. to 13 Oct2000.) in Technical Manual ppat Centre of Advanced Studies in
Plant Pathology Eds.P. N. Chowdhary, Shashi P. Lal, Nita Mathur and D. V. Singh).
73. Sharma, Pratibha, P. N. Chowdhary and Leena Singh. 2000. Differentiating characters
in Peronosporaceae and Albuginaceae. In Training Programme on Identification of Plant
Pathogenic and Biocontrol fungi (14 Sept. to 13 Oct2000.) at Centre of Advanced Studies
in Plant Pathology Eds(.P. N. Chowdhary, Shashi P. Lal, Nita Mathur and D. V. Singh),
pp .23-31
74. Sharma, Pratibha. 2000. Role of Biocontrol in Integrated Disease Management. In
Training Programme on Identification of Plant Pathogenic and Biocontrol fungi (14 Sept.
to 13 Oct.) 5 Oct. at Centre of Advanced Studies in Plant Pathology.
75. Sharma, Pratibha. 2000. “Biological control of rice diseases”. Rice Training Manual of
National Training Course on Integrated Pest Management of Rice September 4-9, 2000 at
NCIPM, pp120-126
76. Sharma, Pratibha. 2000. Role of Plant Pathologists in commercial ventures. IPS
symposium “Phytopathologists March to next millennium 18th Jan, 2000.
77. Singh A. P, B. Singh, and Sharma, P. 1999. Performance of some new I.A.R.I roses
(Rose Seminar Jan’99).
78. Sharma, Pratibha, and A. P. Singh. 1999. Evaluation of germplasm against die-back.
Ninth Annual meeting (Delhi Zone) Jan. 16 Indian Phytopathological Society, pp. 6.
79. Sharma, Pratibha and A.P, Singh. 1999. Multiple diseases resistance program in Roses
against foliar and flower pathogens Ninth Annual meeting (Delhi Zone) Jan. 16 Indian
Phytopathological Society. pp. 6.
80. Singh, A. P., B. Singh and Pratibha Sharma. 1999. Effect of planting dates, spacing and
varieties of Gladiolus on production of corms and cormlets under low cost polyhouse
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pruning in cut lower roses
National Symposium on Emerging Scenario in Ornamental
Horticulture in 2000A.D. and Beyond (21 and 22 July). Indian Society of Ornamental
Horticulture,Abstract No. II-56.
81. Singh, A. P., B. Singh and P.Sharma. 1999. Effect of pre- monsoon pruning in cut
flower roses National Symposium on Emerging Scenario in Ornamental Horticulture in
2000A.D. and beyond (21 and 22 July). Indian Society of Ornamental Horticulture.
Abstract No. II-56.
82. Singh, A.P., B. Singh and Pratibha Sharma. 1999. Effect of pre-monsoon prunning on
roses. First Asian Regional Rose Conference (10-12th January 1999). The Rose Society
of Rajasthan. P. 72-73
83. Sharma, Pratibha. 1999. Epidemiological study on leaf spot/branch rot of Carnations
National Symposium on Emerging Scenario in Ornamental Horticulture in 2000A.D. and
Beyond (21 and 22 July). Indian Society of Ornamental Horticulture. Abst.IV-7.
84. Singh, A.P., B. Singh and Pratibha Sharma. 1999. A study on multi-storeyed building
in roses. National Symposium on Emerging Scenario in Ornamental Horticulture in 2000
A.D. and Beyond (21st and 22nd July,1999), Abstract No II-47.
85. Singh, A.P., B. Singh and Pratibha Sharma. 1999. A study on behavior building in
roses. First Asian Regional Roses Conference (10-12th January, 1999). The Rose Society
of Rajasthan. P. 72
86. Sharma, Pratibha. 1999. Resistance to Sphaerotheca pannosa in roses. National
Symposium on Emerging Scenario in Ornamental Horticulture in 2000A.D and Beyond
(21 and 22 July). Indian Society of Ornamental Horticulture. Abst IV-6 .
87. Sharma, Pratibha. 1998. Utilization of controlled environments in plant disease
research First National Phytotron Training Program Nov’98.
88. Bhattacharya, A. and Shukla, P. 1995. Changes in some phenol metabolism related
enzyme activities in leaves of Pisum sativum L. under infection of powdery mildew
(Erysiphe polygoni DC). In Proceedings of 2nd European Conference of Grain Legume –
Improving production & Utilization of Grain Legumes, Copenhagen Denmark. pp. 82.
89. Singh A.P, B. Singh, and P. Sharma. 1995. A study of multistoried Budding in roses.
National Seminar on Ornamental Horticulture and Environment held at Calcutta from
Feb. 6-8 1995. The Agri. Hort. Soc. Of India. Abstract no. II-I .
90. Sharma, Pratibha. 1995. Management of Ornamental diseases. Short Course/ Summer
Institute on Production Technology for flower crops of commercial interest held from 19
June to 28 June, 95 at Division of Floriculture and Landscaping, IARI, New Delhi.
91. Singh, A.P., B. Singh and Pratibha Sharma. 1995. A study on multi-storeyed building
in roses. The Indian Rose Annual XVI
.
92. Singh, A.P., B. Singh and Pratibha Sharma. 1995. Effect of pre-monsoon pruning on
roses. National Seminar on Ornamental Horticulture and Environment held from
February 6 to 8th, 1995. The Agri. Hort. Sci. Of India, Calcutta. Abstract No II-24.
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93. Sharma, Pratibha. 1995. Disease of Ornamental Crops and Their management. SCERT
Workshop, Division of Floriculture and Landscaping, IARI, New Delhi, May 95.
94. Shukla, Pratibha and V. Garbe. 1994. Studies on enzyme activity tests for detection of
early infection by Verticillium in oilseed rape. Paper presented in 7th meeting of The
working group on Integrated Control in Oilseed Crop at Zurich Switzerland 24th to 25th
February’94.
95. Shukla, Pratibha. 1990. Diseases of Roses in India. Annual meeting of Indian
Phytopathological Society at Tirupati, Jan.3-5.
96. Shukla, Pratibha and Brijendra Singh. 1990. Diseases of Roses – The changing
Pathogen scenario. International seminar on New Frontiers in Horticultural crops
Bangalore, 25-28 November, pp 98.
97. Shukla, Pratibha, A. Bhattacharya and R. S. Shukla. 1989. Changes in free amino acid
content due to powdery mildew of peas. Annual meeting of Indian Phytopathological
Society. Jan., 1989.
LEAD LECTURES
1. Pratibha Sharma. 2012. Biological control of soil borne pathogens in National
symposium on Blending Conventional and Modern Plant Pathology for Sustainable
Agriculture, w.e.f. 4-6 December, 2012 at IIHR, Bangalore. Pg.118
2. Pratibha Sharma. 2012.Use of Trichoderma in Green Technology in National
symposium on Blending Conventional and Modern Plant Pathology for Sustainable
Agriculture, w.e.f. 4-6 December, 2012 at IIHR, Bangalore pp 22-26.
3. Pratibha Sharma. 2012. TRICHODERMA- Anchorage of basic and applied research.
IPS Zonal Meeting (East Zone), Shantiniketan, West Bengal w.e.f. 5 & 6 , Oct, 2012.
4. Pratibha Sharma. 2012. Participated in the National Consultation on Secondary
Agriculture, Building Agro-based Industries. April 9, 2012. FICCI, Federation House
Tansen Marg, New Delhi.
5. Pratibha Sharma. 2011. Role Biopesticides- crop protection. Conference on AgriBiotechnology, 19-20 December 2011: New Delhi pp-34
6. Pratibha Sharma. 2011. Exploration of Biocontrol Genes from Trichoderma species.
National Symposium on “Microorganisms and Plant Health” and 24th Annual General
Meeting of Indian Phytopathological Society (Eastern Zone), w.e.f. November 04-05,
2011 Department of Botany (DST-FIST Sponsored), Guwahati University, Assam,
India.
7. Pratibha Sharma. 2011. Role of Trichoderma harzianum in Abiotic and Biotic Stresses
and Growth Promotion. National Symposium on Innovative and Modern Technologies
for Agricultural Productivity, Food Security and Environmental Management (Edn: D.P.
Biradar, J. Sangeetha, D.Thangadurai) w.e.f. 22-23 July, 2011,
Society for Applied
Biotechnology (India). Mangalore, Karnataka. P.07.
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8. Pratibha Sharma. 2010. Morphological, biochemical and molecular characterization of
highly efficient Indian Trichoderma isolates. National conference (26th-27th October,
2010): Unravelling multiple facets of biological control in national purview: Trends,
Challenges and Societal Implications & Indian Phytopathological Society zonal meeting
(Mid-Eastern zone); Institute of Agricultural Sciences, Banaras Hindu University,
Varanasi. p.154.
9. Pratibha Sharma. 2009. “Status of Biocontrol in India” National Symposium on ‘Plant
Pathology in the Changing Global Scenario’ Feb 27-28, 2009, NBPGR, New Delhi.
10. Pratibha Sharma. 2009. “Ecological behavior of Biocontrol agents against major soil
borne and foliar pathogens” National Symposium on ‘Climate Change, Plant Protection
and Food Security Interface’ December 17-19, 2009 at BCKV, Kalyani, Nadia, West
Bengal.
11. Sharma et. al. 2009. “Integrated management of bacterial diseases of Brassica oleracea
var. botrytis.”
12. Pratibha Sharma. 2009. “Multiplicity and Reproducibility of Fungal Antagonists in
Bioformulation” National symposium on the Biotechnology In Plant Disease
Management For Sustainable Crop Protection, organized by MACS ARI, Pune & IPS,
New Delhi.
13. Pratibha Sharma. 2009. “Present Status of Biocontrol in India” International
Conference on current trends in biotechnology & implication in agricultural, Feb19-21,
2009 at SVPUAT & SRDA, Meerut.
14. Pratibha Sharma. 2008. “Biocontrol Research: Application, Adoption and Missing
Links” National Conference on ‘Eco-Friendly Approaches in Sustainable Agriculture and
Horticulture Production’, Nov 28-30, 2008. 15. Sharma, Pratibha. 2006. Wilt diseases of cucurbitaceous vegetables: Mechanism and
Management, Winter School on “Emerging trends and strategies for management of
pests and diseases in vegetable crops’’at IIVR Varanasi during December 1-21, 2006.
16. Sharma, Pratibha. 2006. Characterization of Fusarium oxysporum causing wilts in
cucurbits. National Symposium on Recent Trends in Diagnosis and management of
Chronic and Emerging plan, Ghosh,D.K.,Chakrabarty, P. K., Mukewar, P. M. and Das,A.
K.(Eds) held during 23-24 November, at CICR, Nagpur (Maharashtra)
p.19
17. Sharma, Pratibha. 2006. Biological Control of Sclerotial rots of Vegetable Crops. In
National Symposium on “New Frontiers in Plant Pathology” at Kuvempu University,
Shimoga, Karnataka) Indian Society of Mycology and Plant Pathology.
18. Sharma, Pratibha. 2006. Use of Molecular tools for detection of variability in Fungal
Plant Pathogens. National Symposium on Microbial Diversity and Plant Health Problems
by Indian Phytopathological Society.
19. Sharma, Pratibha. 2006. Pathogens of vegetable crops, their diagnosis and
management. Training programme on “Advanced Techniques in Plant disease Diagnosis
and Management” Division of Plant Pathology, IARI.
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20. Sharma, Pratibha, Nazafina, M. Gupta, P.and Sharma, A. 2006. PCR-RAPD of Fungal
plant pathogens. Training programme on “Advanced Techniques in Plant disease
Diagnosis and Management” Division of Plant Pathology, IARI.
21. Sharma, Pratibha. 2006. Integrated disease management in vegetable crops. In state
level seminar on “Enhancing productivity and Quality of vegetable crops”, KVK Ujwa
New Delhi.
22. Sharma, Pratibha. 2006. Focus on Healthy Agriculture in India. National Convention
on Knowledge Driven Agricultural Development: Management of Change ARSSF, New
Delhi.
23. Sharma, Pratibha. 2005. Development of Pesticide Residue Free IPM Packages for
Vegetables. In 7th Indian Agricultural Scientist and Farmers Congress of Bioved
Research Society, Allahabad held at S.V.B.P. University of Agriculture and Technology,
Meerut.
24. Sharma, Pratibha. 2004. Use of Trichoderma as a tool of Biocontrol Research. In Zonal
Symposium on Variability of Plant Pathogens and their management” IPS, as Presidential
Address.
25. Sharma, Pratibha. 2004. Effect of pesticides on ecological Behaviour of Trichoderma.
In National Conference on Role of Biopesticides, Bioagents and Biofertilizers for
Sustainable agriculture and horticulture. pp. 40-41.
26. Sharma, Pratibha. 2004. Implementation of Biological control of Plant diseases in
integrated Disease management system.
In National Seminar on Biotechnological
Approaches for the management of plant diseases at Jaipur. pp. 38.
27. Sharma, Pratibha and Singh, Amerika. 2003. Strategy for integrating antagonistic
organism in crop Disease management in India. In ICAR group Meeting on antagonistic
organism in plant disease Management in India at PDPC Bangalore.
28. Sharma, Pratibha. 2003. Biological Disease Management Levels Against Major
Vegetables. LP-17. In: Souvenir & Abstracts of 5th National Seminar on Biological
Agents for Sustainable Management of Pests held at Pant Nagar, Uttranchal. p. 23.
29. Sharma, Pratibha. 2003. Potential of bioagents in plant disease management. In special
session of National Seminar on Emerging trends in plant diseases management organized
by IPS (Mid Eastern Zone) and IISR in Souvenir with abstracts. pp. 85-86.
30. Sharma, Pratibha. 2003. “IPM in Agricultural growth” In Special Session of Rural
Technologies. International Seminar on Downsizing technology for Rural Development.
At Bhubaneswer. Organized by TIFAC, and RRL, CSIR Bhubaneshwar.
31. Sharma, Pratibha. 2003. Use of bioagents in sustainable Vegetable production. In
Proceedings of International Seminar on Downsizing Technology for Rural Development
at Bhubaneswer. Organized by TIFAC, and RRL, CSIR Bhubaneshwar (Eds) S.Khuntia,
A.Parida and Vibuti N Misra. Vol. I pp 193-202.
32. Sharma, Pratibha. 2002. Kharif sabjiyon ke rog evem unka upchar. Prasar Doot- Mela
Visheshank. pp. 39-44.
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33. Sharma, Pratibha. 2002. Integrated disease management: The usable packages for rice
and vegetable crops NCIPM.
34. Sharma, Pratibha. 2002. Integrated disease management of rabi crops at Rabi
Campaign- Orientation Programme 2002-2003. Mitigating delayed monsoon Rabi
meeting of ICAR- State at Bhopal.
35. Singh, D. V. and Sharma, Pratibha. 2002. Integrated disease management in
floricultural crops in Commercial Floriculture. Avenues and Scope in North East India.
pp.224-232
36. Sharma, Pratibha. 2002. Use of bio agents with pesticides in plant disease management
at 4th Indian Agricultural Scientists and Farmer’s Congress. C.C.S. University Meerut,
pp 88-89
37. Sharma, Pratibha. 2002. Use of ecologically competent pesticide resistant bioagents in
Integrated Pest Management. National Symposium on Agriculture in Changing Global
Scenario at I.A.R.I., New Delhi by Indian Society of Agricultural Sciences, and ICAR.
pp.389-390
38. Sharma, Pratibha. 2002. Production of biocontrol products at rural women site at
National Workshop on “Role of Biotechnology in Women Upliftment in the new
millennium.” R.B.S. College, Agra pp. 15-16.
39. Sharma, Pratibha. 2001. Effect of Physical factors on bioagents in “Challenges and
Opportunities of biological control in Plant Disease Management” for Challenges and
Opportunities of Biological Control in Plant Disease Management.
40. Sharma, Pratibha. 2001. Mass Production of Bioagents in “Challenges and
Opportunities of biological control in Plant Disease Management” for Challenges and
Opportunities of Biological Control in Plant Disease Management.
41. Sharma, Pratibha. 2001. Impact of Pesticides on Bioagents in “Challenges and
Opportunities of biological control in Plant Disease Management” for Challenges and
Opportunities of Biological Control in Plant Disease Management.
42. Sharma, Pratibha. 2000. Identification of Alternaria species: Host specificity,
Pathogenesis and Systemic Acquired Resistance. In Training Programme on
Identification of Plant Pathogenic and Biocontrol fungi in Centre of Advanced Studies in
Plant Pathology.
43. Shukla, Pratibha. 1990. Diseases of Floricultural crops in A Decade of Research on
Disease of Horticultural Crops. AICRP (1980-89) Group Discussion of Plant Pathologists
working in the Co-coordinated Projects of Horticultural crops held on 14 to 15 June,
1990 pp.103-119
44. Shukla, Pratibha. 1989. Disease of important ornamentals in India. Summer Institute on
Production Technology for Floriculture crops at Division of Floriculture and
Landscaping, IARI, New Delhi, 12-21 June, pp. 55-65.
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