Curriculum vitae: L. Steyn General information Title: Mrs Name: Laurinda Initials: L Surname: Steyn Maiden name: van Niekerk Identification number: 6604090020085 Citizenship: South Africa Gender: Female Race: White Contact Details Organisation: Department Microbial, Biochemical and Food Biotechnology Faculty of Natural and Agricultural Sciences University of the Free State Email Address: steynl@ufs.ac.za Work telephone number: 051 4012585 Work fax number: 051 4443219 Mobile number: 084 6085698 Website address: uovs.ac.za Qualifications Degree/Diploma: Medical Technician (Chemical Pathology), Central University of Technology, Free State (1989) MSc (Biotechnology), University of the Free State (2007) Employment Position: Researcher (Jan 2007 – Current), Assistant Researcher (Apr 2000 – Dec 2006), University of the Free State, Bloemfontein Development Fermentation Laboratory Manager (Jul 1999 – Mar 2000), Development Fermentation Program Coordinator (Jan 1998 – Jun 1999, Development Biotechnologist (Nov 1994 – Dec 1997), AECI Bioproducts, Umbogintwini Laboratory technician (Apr 1990 – Oct 1994), University of the Orange Free State, Bloemfontein Medical Technician (Dec 1983 – Mar 1990), Drs Voigt and Partners, Bloemfontein Research expertise Fields of specialisation: Fermentation technology, Biotransformations, Yeast, Secondary metabolites production, Bioethanol production, Bioprocess engineering, Biotechnological process control, Data processing, Process development, Fungal fermentations Student supervision record Supervisor: Mr GB Akanni, BSc Hons (2008); Mr TAL Mohafa, BSc Hons (2010) Co-Supervisor: Mr OO Kuloyo, BSc Hons (2007), MSc (2008 – Current); Mr GB Akanni, MSc ( 2009 – Current); Mr AM Meyer, BSc Hons (2009), MSc (2010 – Current); Miss A Mahlangeni, BSc Hons (2007), MSc (2009 – Current); Mr TAL Mohafa, MSc (2011 – Current) Conference Outputs du Preez, J.C., Steyn, L., de Smidt, O., Dukunde, A. and Albertyn, J. (2009) Phenotypic characteristics of multiple ADH deletion mutants of Saccharomyces cerevisiae. 27th International Specialized. Symposium on Yeasts. Paris, France, 26-29 August. (Poster) Albertyn, J., de Smidt, O., Dukunde, A., Henn, M., Steyn, L. and du Preez, J.C. (2009) Molecular and physiological aspects of alcohol dehydrogenases in the yeast Saccharomyces cerevisiae. 16th Biennial congress of the South Africa South African Society for Microbiology, Durban, 21-23 September. Steyn, L., O. de Smidt, K. Ntlhokoa, J. Albertyn and J.C. du Preez.(2008) The role of alchohol dehydrogenase 1 and 2 in Saccharomyces cerevisiae. 15th Biennial Congress of the SA Society for Microbiology (Bio-08), Grahamstown, 21-25 January (Poster). du Preez, J.C., de Smidt, O., Steyn, L. and Albertyn, J. (2008) The use of multiple deletion mutants for determining the physiological role of alchohol dehydrogenase isozymes in Saccharomyces cerevisiae. IUMS 2008. Joint meeting of the divisions of the International Union of Microbiological Societies. XIIth International Congress of Mycology. Istanbul, Turkey, 5-9 August. Biographic information Currently working in the research group fermentation biotechnology for 15 years, with special interest in continuous cultures, yeast physiology and the production of metabolites such as bioethanol using filamentous fungi and bacteria, including genetically engineered micro-organisms. Have a particular aptitude for computer usage: rapidly mastering a number of programmes for data management and constructing graphs, statistical analyses, etc. Used small bench-top bioreactors to conduct a series of fermentations and have rapidly learnt to handle all aspects of this research. Well equipped with the setting up of bioreactors on a 15 litre scale. Keen on undertaking new tasks and have a wide range of technical skills. Grasp the requirements for different investigations and able not only to design the required experiment, but also to analyse the generated data. Active with other departmental duties. I have always been interested in research and started my career as a trainee Medical Technician with the impression that working in a laboratory will satisfy my interest. Unfortunately this was not the case as it was basically routine work with no research aspect. After completing my diploma I decided to change my job and started working as a laboratory assistant at the Department of Microbiology and Biotechnology at the University of the Free State. During this time I was also studying for my B.Sc., unfortunately I had a contract position with no benefits. A permanent position at AECI Bioproducts was presented to me and I made a decision to move. During my time at this company I excelled as researcher and fermentation biotechnologist, but was limited for promotion as I had no formal education as a researcher. Due to personal reasons I returned to the University of the Free State and had the opportunity to complete my M.Sc. with coursework in Biotechnology earning a distinction for my MSc script. I currently hold a permanent position as researcher at the University and have registered for my PhD to further my expertise. I have always worked full time and studied part-time. Courses completed: Self-governing, career-planning, career development and general management (July 1994, U.F.S, Bloemfontein) Fundamentals of Growth Kinetics with emphasis on Chemostat Theory presented by Prof. J.C. du Preez (UFS) (February 1995, AECI R&DD, Johannesburg) The use, maintenance and calibration of pH and ion selective electrodes (April 1997, Swiss Laboratories, Johannesburg) OPTIMUS 6 – software program for image analyses (June 1997, UCT, Cape Town) Basic Experimental Design and Response Surface Methodology (July 1997, AECI, R&DD, Johannesburg) The use of 3M Petrifilm organism identification system (August 1997, Merck, Johannesburg) Team Business education program (September 1998, Business Education Design, Johannesburg) Data Processing and Modeling (February 1999, U.O.F.S., Bloemfontein) Industrial Quality Management (Statistical Process Control (SPCC) and HACCP) (May 2001, UFS, Bloemfontein) Microarray Data Analysis (September 2007, NBN, Cape Town) SPSS Introduction (January 2009, SPSS-SA, Bloemfontein), SPSS Data Management & Manipulation (March 2009, SPSSSA, Bloemfontein) STATISTICA intro training (June 2010, StatSoft Southern Africa, Bloemfontein) Special training: First Aid (June 1996, St. Johns Ambulance, Durban) Fermentation operation (February 1997 & January 1998, AECI Bioproducts, Umbogintwini) Team Achievement Associates, Durban) (June 1997, Owen, Adendorff & Basic Fire fighting Extinguisher (November 1998, AECI Operation Services, Umbogintwini) Microsoft Excel – Intermediate Bioproducts, Umbogintwini) Hazard Studies including Engineering, Johannesburg). HAZOP (March 1999, AECI (May 1999, AECI Represented: At AECI Bioproducts I also represented the company as Safety,Health and Environmental representative (January 1997 – January 2000) and received training accordingly. Member of South African Society of Microbiology (2001-present)