AP Biology
Web Assignment Lab 6
Name: ______________________
Purpose:
To review for AP Lab 6. This virtual lab will outline the procedure, allow you to
get familiar with the substances being used, and give you insight on what to expect from
the in-class lab.
Procedure:
1) Go to the following url:
http://www.phschool.com/science/biology_place/labbench/index.html
2) Go to Lab 6: Molecular Biology
3) Record the purpose of the of the lab in the space below (found in Introduction):
4) What are the titles of the 2 labs that you will perform
A.
B.
5) When does genetic transformation occur?
6) Which bacteria is used in the first lab activity?
7) What is the most common bacterium in the human gut? Name 4 facts about
that bacterium:
8) What are plasmids?
9) Describe the growth rate of a bacterial culture:
10) Summarize the transformation procedure:
11) What is the correct order of stages of transformation? (letters)
12) What are the correct labeling of your 4 plates?
Plate I
Plate II
Plate III
Plate IV
13) How did the discovery of restriction enzymes make genetic engineering
possible?
14) What will you be doing in the Electrophoresis lab?
15) How do restriction enzymes work?
16) At what temperature and at what temperature are these enzymes incubated?
17) What is gel electrophoresis?
18) Summarize the design of experiment II: