BY 102
Zach Nolen
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I’m a Master’s student in the Thacker lab
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Studying sponge/cyanobacteria relationships
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Teaching Portfolio
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Fill out your notecard with the following:
Name (you wish to be called)
 Major
 Reason taking class (be honest)
 1 thing your looking forward to in this class
 1 thing your not looking forward to in this class
 What’s your favorite food
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Read ahead
Do study guides
Work on lab manual throughout
Keep good notes
Ask questions
Study with others
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Microscopes allow us to view very small
objects
There are three main types of microscopes:
compound light, dissecting, and electron
microscopes.
For this class, we will be working with
compound light microscopes.
Spider
Pollen
Diatoms
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Always carry scope with one hand on the arm
and the other under the base.
Always clean scope when you are done.
Always start on the low objective (red), then
work your way up in magnification.
Never use the coarse adjustment knob when
using an objective higher than red.
If there are any issues with your scope, please
let me know immediately.
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Microscopes are very useful tools for biologists.
Not only can they be used to magnify things,
they can also be used to measure things.
To calculate the total magnification of your
scope, you simply multiply the ocular
magnification x objective magnification
Take a few minutes to complete the chart in
your lab manual.
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For this exercise, we will be using the letter “e”
slides.
You will place the slide on the microscope with
the “e” in the correct orientation, then view it
through the microscope to observe how the
scope transposes images.
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In this exercise, you will be preparing a wet mount
of your own cheek cells.
Make sure that you are using a blank slide.
We will be doing a slightly different procedure
than listed in the book.
After you have prepared your slide, use your
microscope to find a cell on the red objective and
let me check it.
After I check it, you will need to use the highest
objective (blue) to find the cells for your drawing.
After you have finished your drawing, you can
wash your slide off in the sink. You will be using
the same slide for other exercises.
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For this exercise, you will be
preparing a wet mount of an
Elodea leaf to observe plant cells.
Elodea is a freshwater plant that is typically
found in ponds.
Follow procedure in the book for slide prep.
You will draw this using the blue objective
After you finish your drawing, you will want
to add a few drops of NaCl to the leaf and
observe plasmolysis.
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This exercise is demo that I will do to show the
process of osmosis.
What is osmosis?
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Osmosis is simply the movement of water molecules
through a selectively permeable membrane from an
area of higher concentration to an area of lower
concentration.
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This exercise is just a review of the various
parts of plant and animal cells as well as their
functions.
Figures 1.8 and 1.9 in your manual have
labeled images of both.
You should be able to identify the different
parts and also be able to give their function.
1.
What are the 3 types of microscopes?
2.
Which type will we use in lab?
3.
What are 3 differences between animal and
plant cells?
4.
What is osmosis?
5.
What is the purpose of mitochondria in the
cell?
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Make sure you properly store your microscope
Clean up your work station
Wipe down your station
Turn in your microscope drawings
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Read Lab exercise 2: Nutrition and the Cell
Do the online prelab
Read the introduction in your manual (viii-xi)
Complete all questions in Lab 1 exercises that
we covered, if not already done so