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Exercise 9
Introduction to the Eukaryotic Cell
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Announcements
 Students
have time to observe Week 8 plates
and finish their LNAs.
 Post
Lab 9/Pre Lab 10 are due by the time
your lab meets next.
 LNA Eukaryotic
Cell is due next week in lab.
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Goals
 To
learn the basic structures associated with
eukaryotic cells.
 To
identify the differences between animal and
plant cells.
 To
understand the basis of cell culture, and the
changes imposed on cells under culture
conditions.
 To
understand the techniques of fluorescence
and electron microscopy and cell culture.
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Animal Cell
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Plant Cell
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The Exercise

Part I: Plant cells: Onion epidermis

Basic cell structure of a plant lacking chloroplasts

Iodine: observe nucleolus

Janus Green: observe mitochondria
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The Exercise

Part II: Animal cells: Human squamous epithelium

Observe differences from the plant cells

Do the mitochondria vary in size from those found in
plant cells?
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The Exercise

Part III: Autofluorescence

View Tradescantia under the fluorescent microscope

Chlorophyll pigments autofluoresce
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The Exercise

Part IV: Anacharis Leaf Cells

View a living plant cell with chloroplasts
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The Exercise
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Part V: Electron Micrographs and Cellular Infrastructure

View EM transparencies

Identify organelles

You may want to take notes for future identification (i.e. Exam 2 and
Final)
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Be Able to Identify:
Nucleus
Nuclear envelope
Nuclear pore
Nucleolus
Rough Endoplasmic Reticulum
Ribosome
Mitochondria
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Be Able to Identify:
Golgi complex
top view
section view
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Golgi
top view
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Golgi
section view
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Be Able to
Identify:
Mitochondrion
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Be Able to
Identify:
Nucleus
Vacuole
Chloroplast
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Be Able to
Identify:
Thylakoid
+ Summary of Exercise 9
 Part


slide x 3: water / Iodine / Janus Green
size, nucleus, vacuole, cell wall/membrane, chloroplast,
mitochondria (?)
 Part
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III: Tradescantia
TA
Autofluorescence
 Part
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II: Cheek cells
slide x 2: water / Janus Green
size, nucleus, cell membrane, mitochondria (?)
 Part
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I: Onion epidermis
IV: Anacharis
Cytoplasmic streaming