Section 2: Inside the Eukaryotic Cell Bell Ringer During lab when you used a light microscope to view a slide of a eukaryotic cell and found the nucleus of the cell, how did you know that the structure was the nucleus? The Framework of the Cell Eukaryotic cells have an intricate network of protein fibers called the ______________which provides the interior framework of the cell. The cytoskeleton helps the cell ___________, keep its shape, and organize its parts. There are three different kinds of cytoskeleton fibers: microfilaments, microtubules, and intermediate fibers. Cytoskeleton Directing Cellular Activity ________ contains instructions for making proteins which control most of the activity of the cell. The DNA of eukaryotic cells is stored in the _______________. DNA instructions are copied as ______________ messages, which leave the nucleus. In the cytoplasm, ribosomes use the RNA messages to assemble proteins. Nucleus A double membrane called the ________________________envelope surrounds the nucleus. Nuclear pores located on the nuclear envelope act as channels to allow certain molecules to move in and out of the nucleus. The___________________ is a structure within the nucleus where ribosome parts are made. These ribosome parts are transported out of the nucleus into the cytoplasm where they are assembled to form a complete ribosome. Ribosomes Each ribosome in a cell is made of ___________ and many different proteins. Ribosomes that are suspended in the cytosol are called “____________” ribosomes. Free ribosomes make proteins that remain inside the cell. Ribosomes that are attached to the membrane of another organelle are called “___________” ribosomes. Bound ribosomes make proteins that are exported from the cell. Ribosomes can switch between being bound or free, depending on what proteins the cell needs to make. Protein Processing Some proteins that a cell manufactures are needed _______________ the cell that makes them. Proteins that are sent outside the cell are packaged in vesicles. _______________are small, membrane-bound sacs that enclose the proteins and keep them separate from the rest of the cytoplasm. The __________________________ and Golgi apparatus are organelles involved in preparing proteins for extracellular export. Endoplasmic Reticulum The endoplasmic reticulum, or ER, is an extensive system of internal membranes that moves proteins and other substances through the ______________. The membranes of the ER are connected to the outer membrane of the nuclear ________________. The endoplasmic reticulum is divided into two portions: _____________ER and ______________ ER. The portion of the ER with attached ribosomes is called rough ER because it has a __________________ appearance when viewed with an electron microscope. The portion of the ER with no attached ribosomes is called smooth ER because it has a smooth appearance when viewed with an electron microscope. The ribosomes on the rough ER make proteins that are packaged into _______________. Enzymes of the smooth ER make lipids and break down ________________substances. Golgi Apparatus The Golgi apparatus is a set of flattened, membrane-bound sacs. The Golgi apparatus helps __________, sort, and _____________________ cell products for distribution. Making and Exporting Proteins The ribosomes located on the rough ER make proteins which then cross into the ________________________ of the ER. The ER membrane then pinches off and forms a vesicle around the proteins. Vesicles transport the proteins from the rough ER to the Golgi apparatus, where they are modified by ___________________and repackaged in new vesicles. These new vesicles transport the modified proteins to the cell membrane to be released outside the cell. Lysosomes Vesicles help maintain ____________________ by storing and releasing a variety of substances as the cell needs them. A lysosome is a vesicle produced by the Golgi apparatus that contains enzymes that break down large molecules. Lysosomes ________________ old or damaged organelles and digest food particles to provide nutrients for the cell. Vacuoles A vacuole is a fluid-filled ___________________ found in the cytoplasm of many plant cells. Plant cells contain a large compartment called the __________________________, which stores water, ions, nutrients, and wastes. When water fills the central vacuole, the cell becomes rigid, allowing the plant to stand up (turgor pressure increased). When the vacuole loses water, the cell shrinks, and the plant wilts. Other Vacuoles Some protists have _____________________vacuoles which pump excess water out of the cell in order to control the concentration of salts and other substances. A _________________ vacuole is another type of vacuole. It is formed when the cell membrane surrounds food particles outside the cell and pinches off to form a vesicle inside the cell. Energy Production Cells need a constant source of __________________. The energy for cellular functions is produced by chemical reactions that occur in the mitochondria and chloroplasts. In both organelles, chemical reactions produce _______________________________________ (ATP), the form of energy that fuels almost all cell processes. Chloroplasts A ____________________ is an organelle found in plant and algae cells that uses light energy to make carbohydrates from carbon dioxide and water. Chloroplasts are surrounded by ___________ membranes and have several stacks of flattened sacs where energy production takes place. ________________ cells may have several chloroplasts. Mitochondria Mitochondria are cell organelles that use _________________ from organic compounds to make ATP. Most of the _____________needed by a cell is produced inside mitochondria. Both animal and plant cells contain mitochondria. A smooth outer membrane and a folded inner membrane surround a mitochondrion. ATP is produced by _______________ on the folds (cristae) of the inner membrane. Summary The cytoskeleton helps the cell move, keep its shape, and organize its parts DNA instructions are copied as RNA messages, which leave the nucleus. In the cytoplasm, ribosomes use the RNA messages to assemble proteins. The endoplasmic reticulum and Golgi apparatus are organelles involved in preparing proteins for extracellular export. Vesicles help maintain homeostasis by storing and releasing a variety of substances as the cell needs them. The energy for cellular functions is produced by chemical reactions that occur in the mitochondria and chloroplasts.