An Egg-cellent Osmosis Experiment Background: Homeostasis is the tendency of a system, especially the physiological system of higher animals, to maintain internal stability, owing to the coordinated response of its parts to any situation or stimulus that would tend to disturb its normal condition or function. To maintain homeostasis within cells, water, ions and molecules must travel into, and out of, the phospholipid bilayer. One type of cellular transport is osmosis, or the movement of water into and out of the cell determined by the concentration of solutes in the surrounding environment. Purpose: To understand osmosis you will make observations about an egg. You will by studying the effects different concentrations of fluids on the weight/mass of a deshelled egg. Procedure Stage 1- Deshelling the egg Materials: o 1 cup o 1 egg o Vinegar o Scale o Parafilm 1. Place your egg in your cup 2. Cover the egg with the vinegar 3. Cover the cup with parafilm, let sit for 2-3 days Stage 2- Solutions set up Materials: o Deshelled egg o Cleaned cup o Parafilm o Assigned solution 4. 5. 6. 7. 8. 9. Dump vinegar in sink and gently clean egg clean too harshly and it will break Rinse out cup Weigh the egg on the scale make sure to clean the scale before and after Record the weight in your assigned concentration box on the chart under “Weight before” Put egg back into cup and fill the cup ¾ of the way with your assigned solution, cover in parafilm and let sit for 1-2 days Hypothesis 1: What do you think is going to happen to your egg? Is it going to get larger, smaller, or stay the same? Why? ____________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________ 10. Hypothesis 2: What trend do you predict to see for all the eggs? (look at the data table for all the different solutions) Explain. ____________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________ Stage 3- Final weigh in Materials: o Deshelled egg o Wet paper towels (for cleaning) o Scale 11. Empty solution into the sink 12. Gently pat dry egg rub too harshly and it will break 13. Weigh egg and record the weight in your assigned concentration box on the chart under “Weight after” Graph Scatter plot- dots only, no lines One color for each concentration o Make sure there is a key indicating what color symbolizes which concentration Conclusion Write a formal lab conclusion for this lab Make sure to include a data table and a graph in your paragraph. Data TableNames of Group Members Assigned Solution Group 1 100% syrup Group 2 80% syrup Group 3 60% syrup Group 4 40% syrup Group 5 20% syrup Group 6 Group 7 Group 8 Group 9 Group 10 20% vinegar 40% vinegar 60% vinegar 80% vinegar 100% vinegar Benson Reg. Water Mass of Egg Before Mass of Egg After Change in weight % Change