QUICKLY WRITE IN YOUR PLANNE R AND BE READY TO START THE LAB. Is Yeast Alive? How to Set Up the “Is Yeast Alive?” Lab Materials and Equipment 4 Test Tubes Test Tube Rack Yeast (1 cup) Beaker 4 Balloons Sugar (2 packs) STEP 1 Beaker Fill a beaker with 40 mL of warm water from Mrs. Plankenhorn. STEP 2 Add the yeast from the white paper cup A LITTLE BIT AT A TIME into the 40 mL beaker of warm water— STIRRING the yeast thoroughly before adding more. 40 mL Pour yeast solution from the beaker into each of the four test tubes, filling evenly (about 10 mL in each tube. Step 4: Sugar Packet Test Tube 1 Test Tube 2 Add one packet of sugar to test tube 1 and one packet of sugar to test tube 2. Do NOT add sugar to test tube 3 or test tube 4! Step 5: With 1 person… Finished… With 2 people… Cover the opening of each test tube with a balloon to trap any gas. This can either be done by one or two people. (see examples above) Step 6: Your thumb... Hold your thumb over the end of each test tube and shake it vigorously until the contents are completely mixed. Test Tube with balloons Pink = with sugar Orange = NO sugar Step 7 Step 8: Remember to write down all observations. Make detailed sketch of the setup during the last five minutes. Return test tubes to the rack and record initial observations on your lab paper. Then, every 5 minutes observe and record any changes. Include additional sketches and written observations.