*Is yeast alive?(can be used as demo or lab) Objective: Observe the product of a metabolic pathway Observe metabolism(one of the characteristics of life) Distinguish between living and non-living objects Materials:500ml beaker 100ml beaker Lrg. test tube Sm. test tube (needs to fit inside lrg. tube) Hot plate Yeast Sugar Thermometer Procedure:1. Prepare a fresh lukewarm yeast solution 2. Add some sugar to the solution and stir 3. Set up a warm-water bath 4. Put one beaker inside the other 5. Keep temperature of bath at about 380C 6. Fill the large and small test tubes to the top with yeast solution. 7. Turn the sm. tube upside down and place it inside the lrg. test tube.( try not to trap air bubbles) 8. Put the “double test tube” setup in the sm. beaker 9. Let setup sit for about 10 to 15 min. while students observe 10. Tell students the solution also contains sugar 11. Ask the students to tell you what they are observing . You can ask students to give reasons for what they are observing. Bubbles form in the bottom of the small test tube because the yeast cells are using the sugar in a metabolic pathway called cellular respiration . As a result, carbon dioxide gas is produced. *Demonstration taken from Modern Biology, Chapter 1 pg. 12