What research led to the discovery of DNA as hereditary material?
Frederick Griffith 1928
Avery and McCarty, 1944
Experiment:
Experiment:
Outcome:
Outcome:
Hershey and Chase, 1952
Erwin Chargaff, 1950
Experiment:
Experiment:
Outcome:
Outcome:
Wilkins and Rosalind Franklin
Watson and Crick, 1953
Experiment:
Experiment:
Outcome:
Outcome:
DNA Replication:
Analysis Questions:
1. ) A biochemist isolates, purifies, and combines in a test tube a variety of molecules needed for
DNA replication. When she adds some DNA to the mixture, replication occurs, but each DNA
molecule consists of a normal strand paired with numerous segments of DNA a few hundred
nucleotides long. What has she probably left out of the mixture? Explain.
a. DNA polymerase b. DNA ligase
c. nucleotides
d. Okazaki fragments e. primase
2.
Why do DNA molecules get smaller after each round of replication?
3.
The template strand of a gene contains the sequence 3’TTCAGTCGT5’. Identify the
non-template sequence and mRNA sequence. Include the 3’ and 5’ directionality.
4.
Compare DNA polymerase and RNA polymerase. Discuss primers, the direction of synthesis,
and the type of nucleotides used.