-Types of
Solutions
-Facilitated
Diffusion
-Active Transport
1.
2.
3.
4.
What is diffusion?
What is osmosis?
Draw a picture of the same
concentration of solutes
inside a cell as outside a cell.
Draw a hyper person.
Have data out and ready to
hand in


Is also IMPERMEABLE to sucrose…
Which is good, because I made a bunch of
sucrose solutions of different
concentrations, and I can’t remember
which is which.
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
Objective:
To determine which of the
unknown solutions is High, Medium and
Low concentration.
Purpose: To observe the process of
osmosis first hand and to use this process
to understand how dialysis works.
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3 Unknown colored solutions (High, Med
and Low Concentration)
Water (150 mL)
3 beakers*
3 pieces of dialysis tubing*
3 pipettes
Scissors
Scale
Paper towel
PROCEDURES: Watch demo, and write down
procedures

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Lab Title: Osmosis Lab (+ catchy title)
Problem/Question: Does ______________ of
_________ in the bag affect the final ________ of
the bag in ___ after in soaks in water.
Hypoth:
if _________________ (color solution) has
(highest/lowest solute concentration) relative to
the
Then _________________
As measured by __________
COLOR
INITIAL
FINAL
Difference
High/Low
/Med
Red
Blue
Yellow
Diagram:
Draw all 3 set ups at the start of the experiment and 15 minutes later.
-Include particulate drawings showing H20, and sucrose molecules.
-Label the solute and the solvent.
-Use arrows to show the flow of water

Materials:

Procedures: 

Data Table:

Qual. Observations
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
Graph: line/bar
Conclusion
 Iso
– same
 Hypo – Low
 Hyper – High
 Tonic
– Liquid/Solution

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Hyper= more/too much
Higher concentration solution outside the cell


Iso= same
Same concentration outside the cell as inside


Hypo= less/too little
Lower concentration solution outside the cell
 Oxygen
and Carbon
dioxide enter and leave the
cell through simple
diffusion
 Small
unpolarized (not
ions) molecules can
pass through the cell
membrane without a
protein channel.
Lets revisit our
salt water
example
 According to
simple
diffusion, draw
what will
happen here.


The solute will
spread out until
it is evenly
dispersed.

Draw what happens to a cell with lots of
solute in it, dropped in a beaker of pure H2O?
Remember that the cell membrane is
permeable to water but not solutes.

Water follows solute into the cell… and the
cell swells up.

If it swells up too much, the cell can burst if
the membrane gets pulled apart.
 FACILITATED
DIFFUSION
 (another easy way – NO energy
required!)
 molecules Glucose, sodium ions and
chloride ions
need help (facilitated)
getting across membrane
 Carrier
proteins help
 Energy
(ATP) required for
movement.
 Solutes are moved AGAINST
the concentration gradient.
(from low to high conc.)

Look back at your notes for
Passive and Active Transport.
List two differences between
passive transport and active
transport.
Name one thing they have in
common!

Differences
 Active Uses ATP- This is the energy source for the
cell.
 Active goes against the gradient

Similar
 Both have carrier proteins
 Both move molecules that cannot go through the
membrane on their own
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Endocytosis- cell takes in substances
Exocytosis- cell releases substances (out)
ACTIVE TRANSPORT – requires ATP (energy)
A
portion of the cell membrane
surrounds the desired molecule
outside the cell.
 The cell membrane rejoins
pinching off a sac-like organelle
called a vesicle.
 There are 2 types of endocytosis:
phagocytosis and pinocytosis.
 The
reverse of endocytosis
 Wastes and cell products are
packaged by the golgi body in
sacs called vesicles.
 These vesicles fuse with the cell
membrane and the materials are
secreted out of the cell.
Cellular Transport
Requires ______
Cellular Transport
Transport
ATP
Requires ______
PASSIVE
ACTIVE
Facilitated
Diffusion
Diffusion
Proteins
and ATP
Osmosis
Oxygen &
Carbon Dioxide
Hypertonic
Hypotonic
Isotonic
Glucose
transport
Passive Transport:
Diffusion:
Osmosis:
Hypertonic solution:
Hypotonic solution:
Isotonic solution:
Na+/K+ Pump
Active Transport:
Facilitated diffusion:
Glucose transport:
Na+/K+ Pump:
Endocytosis
Exocytosis
Solution:
Solute-particle
Solvent-liquid
Passive vs. Active
Diffusion
Osmosis
ENDO
EXO
Your
Crazy
Study
Page For
Mon’
Test
Don’t forget:
Draw a
phospholipid
bilayer with
proteins and
carbohydrates
(& their
functions)
1. What is the difference between
active and passive transport?
2. LIST 2 kinds of passive transport.
3. With a concentration gradient,
molecules move from areas of ____
concentration to areas of ____ conc.
4. During osmosis, if there is more
salt inside the cell than outside,
which way would water move?