Exam II Practice Exam: Chapter 5,6,7,8,11 Supplemental Instruction Iowa State University Leader: Course: Instructor: Date: Adam Biology 211 Emeka Kemdirim 1. Reactions that release free energy are a. Exergonic b. Spontaneous c. Endergonic d. Endothermic e. Both a and b 2. Circle all of the following that are true in regards to the basic framework of a plasma membrane? a. The membrane is made of a phospholipid monolayer. (bilayer) b. Proteins may span the membrane c. Proteins may be bound on the surface to other protein d. Proteins may be bond on the surface to lipids e. Proteins which have covalently bound carbohydrates are called glycoproteins f. Lipids which have ionic bonded carbohydrates are called glycolipids. (covalent bond) 3. Which enzyme alleviates coiling ahead of the replication fork? a. DNA topiosmerase b. DNA ligase c. DNA primase d. DNA polymerase II 4. Light energy is captured by chlorophyll and converted to chemical energy in the form of ATP and NADPH. This takes place where? a. Stroma b. Thylakoid c. Intermembrane space of chloroplast d. Mitochondria 5. The tendency for Na+ to move into the cell could be due to a. The higher numbers of Na+ outside the cell, resulting a chemical concentration gradient b. The net negative charge inside the cell attracting the positively charged Na+ c. The attractive force of K+ inside the cell pulling NA+ into the cell d. A and B only 6. Facilitated transport requires a. Membrane channel or carrier protein b. ATP hydrolysis c. H+ cotransporter d. Both A and B 7. Which of the following is not an integral membrane protein? a. Transmembrane protein b. Lipid anchor protein (lipid covalently attached to an amino acid side chain) c. Peripheral protein d. None of the above Supplemental Instruction 1060 Hixson-Lied Student Success Center 294-6624 www.si.iastate.edu 8. Plants commonly found in hot and dry environments that carry out carbon fixation at night are a. C3 plants b. CAM plants c. C4 plants d. Nocturnal plants 9. If the extracellular fluid surrounding a plant cell is hypertonic, water will… a. Exit the cell and the plasma membrane will pull away from the cell wall b. Move into the cell and the plasma membrane will inflate c. Move in and out in equal amounts d. Exit the cell and the plasma membrane will inflate 10. For the answer in the question above, what is this process known as? a. Crenation b. Plasmolysis c. Lymph loss d. Isotonic 11. Label each molecules with either a high,moderate, low, and very low permeability across the plasma membrane. (use each only once) i. Water M ii. CO2 H iii. DNA (charged polar) VL iv. Glucose (polar organic molecules) L 12. List all the products of the Citric Acid Cycle i. 2 ATP ii. 6 NADH iii. 2 FADH iv. 4 CO2 13. Water is necessary for photosynthesis because a. It is split into H2 and O2 b. It is directly involved in the synthesis of carbohydrate c. Provides the electrons to replace lost electrons in PSII d. None of the above 14. Which of the following statements about the 5’ end of a nucleic acid is correct? a. The 5’end has a phosphate group b. The 5’end has a Hydroxyl group c. The 5’end has a Carbonyl group d. The 5’end has a is identical to the 3’ end 15. Glycolysis can still occur and make small amounts of ATP even with oxgen, for muscle cells what is the final product of fermentation, and what is the final product for yeast? a. Lactate, glucose b. Fructose, glucose c. Lactate, ethanol d. Pyruvate, Co-enzyme A 16. In biological systems, ATP functions by a. Providing the energy to drive endergonic reactions b. Acting as an enzyme and lowering the activation energy of certain reactions c. Adjusting the pH of solutions to maintain optimal conditions for enzyme activity d. Regulating the speed at which endergonic reactions proceed e. Interacting with enzymes as a cofactor to stimulate chemical reactions 17. A transporter that moves two solutes in the same direction is called… a. A Uniporter b. A Symporter c. A Antiporter d. A Sameporter 18. Substances can move across the membrane in three ways, describe each including the direction of movement in regards to concentration gradient and if it requires energy. Diffusion: occurs when a substance moves from a region of H to L Facilitated diffusion: transport protein provides a passageway for a substance to cross the membrane. 1 and 2 are examples of passive transport. Active transport: L to H against concentration gradient. Require ATP 19. With regard to its effect on an enzyme-catalyzed reaction, a competitive inhibitor a. Lowers the Km only b. Lowers the Km and lowers the Vmax c. Raises the Km only d. Raise the Km and lowers the Vmax 20. During fermentation why is it necessary to convert pyruvate into its respected end product? a. To decrease NAD+ and increase NADH b. To decrease NADH and increase NAD+ c. To increase NADH and increase NAD+ d. To decrease NADH and decrease NAD+ 21. What model was the observed method of DNA replication from the Meselson and Stahl experiment? a. Conservative b. Semi-conservative c. Dispersive d. Conjugated 22. Which enzyme covalently bonds the parent and daughter strand together? a. DNA topiosmerase b. DNA ligase c. DNA primase d. DNA polymerase II 23. Which of the following pairs of base sequences could form a short stretch of a normal double helix of DNA? a. 5’-A-G-C-T-A-C-3’ & 5’-A-G-C-T-A-C-3’ b. 5’-A-G-C-T-A-C-3’ & 5’-C-A-T-C-G-A-3’ c. 5’-A-G-C-T-A-C-3’ & 5’-G-T-A-G-C-T-3’ d. 5’-A-G-C-T-A-C-3’ & 5’-T-C-G-A-T-G-3’ 24. Let’s suppose an insect, which doesn’t maintain a constant body temperature, was exposed to a shift in temperature from 60 degrees F to 80 degrees F. Which of the following types of cellular changes would be the most beneficial to help this animal cope with the temperature shift? a. Increase the number of double bonds in the fatty acyl tails of phospholipids b. Increase the length of the fatty acyl tails of phospholipids c. Decrease the amount of cholesterol in the membrane d. Decrease the amount of carbohydrate attached to the membrane protein e. Decrease the amount of carbohydrate attached to phospholipid 25. Describe the spontaneous movement of the lipid bilayer (name the three ways lipids move). Rotate, lateral, flip flop-> require energy. 26. Which form of movement is characterized by the formation of membrane vesicles from plasma membrane as a way for cells to internalize the extracellular fluid? a. Exocytosis b. Endocytosis c. Pinocytosis d. Phagocytosis 27. Which of the following is not a products of glycolysis a. Pyruvate 2 b. ATP 2 c. NADH 2 d. FADH 28. Which of the following statements is incorrect? a. Glycolysis takes place in the cytoplasm b. Citric Acid cycle takes place in the mitochondrial matrix c. Oxidative phosphorylation takes place in the mitochondrial inner membranes. d. None of the above 29. Of the following statements, which is correct when considering the process of DNA replication? a. New DNA molecules are composed of two completely new strands b. New DNA molecules are composed of one strand from the old molecules and one new stand c. New DNA molecules are composed of the old molecules and sections that are new d. New DNA get a random distribution of parental and daughter strand DNA 30. RNA primers are removed and filled in with DNA by which enzyme? a. DNA polymerase I b. DNA polymerase II c. DNA polymerase III d. DNA Helicase 31. The Na+ and K+ pump does NOT a. Transport Na+ out of the cell b. Transport K+ into the cell c. Requires ATP d. Transports equal amounts of Na+ and K+ per cycle 32. Photorespiration is avoided in C4 plants because a. These plants separate the formation of four carbon molecules from the rest of the Calvin cycle in different cells b. These plants carry out only anaerobic respiration c. The enzyme PEP functions to maintain high CO2 concentrations in the bundlesheath cells d. Both A and C 33. How many ATP are produced by each pyruvate during glycolysis a. 1 b. 2 c. 3 d. 4 34. When water leaves a cell and causes it to shrink, this is termed… a. Dehydration b. Drying c. Crenation d. Osmotic lysis 35. Integral membrane proteins bound within the membrane can be removed by which of the following? a. High Salt concentration b. High ATP hydrolysis c. Dissolving in Detergent (organic solvent) d. A lot of shaking. (like a Polaroid picture) 36. 30-34 ATP are produced during Oxidative phosphorylation in cellular respiration through which energy yielding mechanism? a. Substrate-level phosphorylation b. Chemiosmosis 37. The Calvin cycle for photosynthesis takes place where? a. Thylakoid b. Granum c. Stroma d. Intermembrane space of chloroplast 38. Which enzyme separates the two strands of DNA as it moves outwards from the orgin of replication? a. DNA polymerase I b. DNA polymerase II c. DNA polymerase III d. DNA Helicase 39. DNA moves in which directionality? 40. Certain drugs can cause the mitochondrial membrane to be highly permeable to H+. How would such drugs affect oxidative phosphorylation? a. Movement of electrons down the ETC would be inhibited b. ATP synthesis would be inhibited c. ATP synthesis would be unaffected d. ATP synthesis would be stimulated 41. Primary active transport involves the use of pre-existing gradients to drive the active transport of another solute while secondary active transport involves a pump that directly uses energy. a. True b. False 42. Which compound does the Citric Acid cycle begin with and end with, for this reason it is called a cycle. a. Phosphofructokinase b. Co-enzyme A c. Oxaloacetate d. FADH 43. Which gas enters the stomata and which leaves? a. Oxygen, Nitrogen b. Oxygen, Carbon dioxide c. Carbon dioxide, Oxygen d. Sulfur, oxygen 44. Okasaki fragments occur on which DNA strand? a. Leading b. Lagging 45. Which of the following is not true in regards to entropy? a. Entropy should always increase in a system b. Entropy refers to the disorder in a system c. The 2nd law of thermodynamics refers to entropy and the measure of randomness in a system d. Entropy should always decrease in a system so biological systems become more organized 46. Transporters bind their solutes in a hydrophilic pocket and undergo a conformational change that switches the pocket from one side to another. a. True b. False 47. Which variation of photosynthesis is best suited for hot and dry environments? a. C4 plants b. CAM plants 48. In a chemical reaction, NAD+ is converted to NADH. This is called ________. When NADH is converted to NAD+ and H+ this is called _______. a. Oxidation, Reduction b. Reduction, Oxidation 49. Where does the oxygen for expelled water come from? a. CO2 b. H2O 50. If you have any questions you can do all of the following: a. Raise your hand (Adam doesn’t like asking for questions to get no response) b. Wait till the second half of the review time which will be devoted to any questions. c. RAISE YOUR HAND d. All the above