BELL RINGER 9-16-15 1. In an experiment involving an enzyme, the pH is raised from 2 to 11. What will be the MOST LIKELY effect of this on the reaction? a. b. c. d. 2. Which organelle is responsible for making proteins? a. b. c. d. 3. The enzyme will denature, stopping the reaction The activation energy of the reaction would be reduced The rate of reaction will reduce due to a decrease in the kinetic energy of the molecules The rate of reaction will increase due to the increased number of collisions of the enzyme and substrate Ribosomes Chromosomes Chloroplasts Golgi apparatus Substances that enter the cell without the expense of cellular ATP, but are too large to directly pass through the membrane use _____________________ in order to gain access to the inside of the cell. a. b. c. d. Osmosis Vesicles Pseudopodia Membrane proteins AGENDA Standard Key Ideas Notes on DNA DNA Modeling DNA coloring STANDARD SB2. Students will analyze how biological traits are passed on to successive generations. b. Explain the role of DNA in storing and transmitting cellular information KEY IDEAS What is genetic material composed of? What is the shape of the DNA molecule? How is information organized and stored in a DNA molecule? WHAT DOES THIS HAVE TO DO WITH MENDEL? Mendel did his work with pea plants in the 1800s - well before scientists knew what was responsible for the passing down of traits Mendel studied. WHAT DOES THIS HAVE TO DO WITH MENDEL? Since then, scientists have discovered that genes are the instructions for inherited traits. A gene is a segment of DNA located on a chromosome and it codes for a specific hereditary trait. By the way, it wasn’t until the 1950s that scientists discovered that genes are made up of DNA. DNA is the primary material that causes recognizable, inheritable characteristics in related groups of organisms THE SHAPE OF DNA James Watson and Francis Crick were the first to put together a model of DNA’s structure. So, what does it look like? We call this a double helix (or a spiral staircase) THE SHAPE OF DNA DNA is composed of two parallel strands of linked subunits (called nucleotides) Nucleotides are made up of three parts: a phosphate group, a five-carbon sugar molecule, and a nitrogencontaining base. DNA STRUCTURE If we were to unwind all of the DNA in just one of our cells it would be over 6 feet long! How does that fit in a microscopic cell? It’s all in the twist! DNA STRUCTURE Why doesn’t DNA get tangled up if there’s so much of it in a cell? Parallel strands! Do parallel lines ever cross? HOW DOES THAT HOLD INFO? The Nitrogen Bases 4 different nitrogen bases: adenine (A), guanine (G), thymine (T), cytosine (C) A and G are purines. Purine – a nitrogen base that has a double-ring structure T and C are pyrimidines. Pyrimidine – a nitrogen base that has a singlering structure HOW DOES THAT HOLD INFO? Base-Pairing Purine with Pyrimidine ….. So, A with T and G with C. Pairing happens across the parallel strands Bases are held together by weak hydrogen bonds, this keeps the DNA strand together Paired bases are said to be complementary because they fit together like puzzle pieces. HOW DOES THAT HOLD INFO? If the sequence on one strand is TATGAGAGT, the sequence on the other strand must be ATACTCTCA. Pairing ensures that each strand of the DNA molecule contains the same information (the information on one is just the reverse of the information on the other). BASE PAIRING PRACTICE Given the following strands, write the complementary strand AGGCCTAT TCCGGATA TTCCGGAA AAGGCCTT GACGACTA CTGCTGAT ATGCGCAT TACGCGTA DNA MODELING To help you get a better idea of what DNA looks like we’re going to model it.