Abstract template for “The 3rd Sapporo Summer Seminar for One Health (SaSSOH), 2015”
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Xxxxx YYYYY1, Xxxxx YYYY2, Xxxxx YYY1
Lab. Xxxxx, Graduate School of Yyyyyy, Hokkaido University.
2
Div. Yyyy, Research Center for Zoonosis Control, Hokkaido University.
XXXXX@yyyyy.hokudai.ac.jp
1
Xxxxxxx……
Title
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Background,
Materials
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Methods,
Results,
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Example. 1
Detection and isolation of novel phleboviruses from ticks in Japan
Ryo NAKAO1, Masahiro KAJIHARA2, Keita MATSUNO3, Yongjin QIU4,
Akina MORI2, Naganori NAO2, Kentaro YOSHII5, Hiroaki KARIWA5,
Hirofumi SAWA6,7, Chihiro SUGIMOTO4,7, Ayato TAKADA2,7, Hideki
EBIHARA3
1
Unit. Risk Analysis and Management, Research Center for Zoonosis Control,
Hokkaido University.
2
Div. Global Epidemiology, Research Center for Zoonosis Control, Hokkaido
University.
3
Lab. Virology, Division of Intramural Research, National Institute of Allergy
and Infectious Diseases, National Institutes of Health, Rocky Mountain
Laboratories, Hamilton, Montana, USA.
4
Div. Collaboration and Education, Research Center for Zoonosis Control,
Hokkaido University.
5
Lab. Public Health, Graduate School of Veterinary Medicine, Hokkaido
University.
6
Div. Molecular Pathobiology, Research Center for Zoonosis Control,
Hokkaido University.
7
School of Veterinary Medicine, the University of Zambia, Lusaka, Zambia.
XXXXX@czc.hokudai.ac.jp
Introduction
Since the emergence of severe fever with thrombocytopenia syndrome virus
(SFTSV), an increasing attention has been paid to tick-borne phleboviruses
circulating in Asian countries. This study aimed at the detection and
characterization of tick-borne phleboviruses harbored in different tick species
in Japan.
Method and Results
A total of 817 ticks, consisting of 16 species, were collected in Japan in 2013.
We employed a recently developed one-step RT-PCR system to detect a wide
range of phleboviruses. A total of 27 individual ticks, including 5
Haemaphysalis flava, 3 Haemaphysalis longicornis and 19 Ixodes persulcatus,
were tested positive by RT-PCR. Sequencing analysis of amplified PCR
products indicated that the obtained sequences formed distinct clades from
those of previously reported phleboviruses including SFTSV. To further
characterize these novel viruses, virus isolation was attempted using both
mammalian (DH82, Huh7 and Vero) and tick (ISE6) cell lines. The viral
sequences were recovered only from ISE6 cell culture at 7 days after
inoculation of two phlebovirus-positive I. persulcatus samples. The viral RNA
purified from the culture supernatant was subjected to pyrosequencing. After
de novo assembly of the resulting sequence reads followed by manual gap
closing, whole genome sequences of L, M and S segments were obtained.
Conclusions
This study confirmed the presence of diverse phleboviruses circulating in
Japan. Further studies are under way to investigate their pathogenicity to
mammals and antigenic cross-reactivity with known pathogenic phleboviruses.
Title
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letter should be capital
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Affiliation
 Name of Laboratory/Division
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Contact information
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Abstract text
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 The text body can be divided into
several
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Background,
Materials
and
Methods,
Results,
and
Conclusions.
 References should not be included.
 Not exceed 300 words.
Example. 2
Comparison of the anti-prion mechanism of four different anti-prion
compounds in prion-infected mouse neuroblastoma cells
Takeshi YAMASAKI1, Akio SUZUKI1, Rie HASEBE1, Motohiro
HORIUCHI1
1
Lab. Veterinary Hygiene, Graduate School of Veterinary Medicine,
Hokkaido University
XXXXX@vetmed.hokudai.ac.jp
Molecules that inhibit the formation of an abnormal isoform of prion protein
(PrPSc) in prion-infected cells are candidate therapeutic agents for prion
diseases. Understanding how these molecules inhibit PrPSc formation provides
logical basis for proper evaluation of their therapeutic potential. In this study,
we extensively analyzed the effects of the anti-PrP monoclonal antibody
(mAb) 44B1, pentosan polysulfate (PPS), chlorpromazine (CPZ) and
U18666A on the intracellular dynamics of a cellular isoform of prion protein
(PrPC) and PrPSc in prion-infected mouse neuroblastoma cells to reevaluate the
effects of those agents. MAb 44B1 and PPS rapidly reduced PrPSc levels
without altering intracellular distribution of PrPSc. PPS did not change the
distribution and levels of PrPC, whereas mAb 44B1 appeared to inhibit the
trafficking of cell surface PrPC to organelles in the endocyticrecycling pathway
that are thought to be one of the sites for PrPSc formation. In contrast, CPZ and
U18666A initiated the redistribution of PrPSc from organelles in the
endocyticrecycling pathway to late endosomes/lysosomes without apparent
changes in the distribution of PrPC. The inhibition of lysosomal function by
monensin or bafilomycin A1 after the occurrence of PrPSc redistribution by
CPZ or U18666A partly antagonized PrPSc degradation, suggesting that the
transfer of PrPSc to late endosomes/lysosomes, possibly via alteration of the
membrane trafficking machinery of cells, leads to PrP Sc degradation. This
study revealed that precise analysis of the intracellular dynamics of PrPC and
PrPSc provides important information for understanding the mechanism of antiprion agents.
Title
 Not exceed 15 words
 Bold
Author’s name
 First and middle name: Only first
letter should be capital
 Family name: All capital
 Under line for the presenter
 Author
affiliation
should
be
numbered
 Bold
Affiliation
 Name of Laboratory/Division
 Name of Graduate School
 Name of University/Institute
Contact information
 E-mail address of the presenter
Abstract text
 Images (tables, figures etc.) cannot
be embedded.
 The text body can be divided into
several
sections
such
as
Background,
Materials
and
Methods,
Results,
and
Conclusions.
 References should not be included.
 Not exceed 300 words.