Biochemistry STUDY GUIDE KEY 1. What are the 4 biomolecules of life? Give an example of each. Protein - meats Lipids – fats and oils Carbohydrates – breads Nucleic Acids – DNA & RNA 2. Complete the following table. Macromolecule Monomer/Polymer Elements/Components involved Examples Protein Amino acid/ polypeptide C, H, O, N Carboxyl group, amino group, R group Enzymes, meats, hair Lipids Fatty acids, glycerols/ Triglycerides C, H Glycerol, Fatty acids, phosphate group (phospholipids) Wax, Steroids, fats, oils Carbohydrates Monosaccharide/ Polysaccharide C, H, O Bread Nucleic Acids Nucleotide/ DNA & RNA C, H, O, N, P Phosphorous group, sugar, nitrogenous base DNA, RNA Indicator Biuret Pos: purple Neg: blue Paper smear Pos: translucent Neg: opaque Starch: Iodine Pos: black N: red/orange Sugar: Benedict’s solution Pos: green-red Neg: Blue N/A 3. Each biomolecule has a single unit known as a __________and a many unit structure known as a _________. Each biomolecule has a single unit known as a monomer and a many unit structure known as a polymer. 4. If two monomers of carbohydrates were to bond it would make a _______. If two monomers of carbohydrates were to bond it would make a disaccharide. 5. Which 3 elements do carbohydrates contain? Carbon Hydrogen Oxygen 6. Describe a biological function for each of the following carbohydrates Cellulose: makes the structure of the cell walls Starch: how plants store polysaccharides for energy 7. Briefly describe what a peptide bond is. A peptide bond is when two or more amino acids link together to make a polypeptide or protein. When a peptide bond is made, one molecule of water is produced. 8. An amino acid has three parts. What are they called? Amino group Carboxyl group R group 9. Enzymes are catalysts. What does that mean? Enzymes start a reaction and help it occur faster. They are necessary for our life functions. Without them, reactions would take too long to take place. 10. The substrate of an enzyme binds to the _____ of the enzyme in order for a reaction to occur. The substrate of an enzyme binds to the active site of the enzyme in order for a reaction to occur. 11. What is denaturing a protein mean? Denaturing a protein means that the protein itself changes shape due to temperature, salinity, or pH and is no longer functional. This reaction is not reversible. 12. What conditions can cause an enzyme to change or denature? High temperatures Salinity pH 13. What are the four types of lipids? Triglycerides Phospholipids Waxes Steroids 14. What is this difference between saturated and unsaturated fats? Saturated fats are from animals, contain only single bonds in the fatty acid chains, and are solid at room temperature. Unsaturated fats are from plants, contain at least one double bond in the fatty acid chains, and are liquid at room temperature. 15. What are the two type of nucleic acids? DNA RNA the end.