Cellular Transport How do cells move nutrients, products and wastes in and out of the cell? Why is this traffic across the membrane so important? Cells are filled and surrounded by watery fluid. This fluid helps solids move around inside, outside the cell and cross the cell membrane. Cell Membrane The cell membrane controls what moves in and out of the cell. Semi-permeable membrane Cell membranes only allow some solutes (solids) to move across it. Movement across the membrane depends on size and type of the solute (solid). Membranes are just so beautiful!!!!! Types of Transport High concentration Low concentration Low concentration Down the concentration gradient High concentration Up the concentration gradient Summary: Types of Transport Passive Transport Molecules move from area of high concentration to area of low concentration Movement is down the concentration gradient No energy needed Small molecules such as H2O, O2 and CO2 Active Transport Molecules move from area of low concentration to area of high concentration Movement is up the concentration gradient Energy is required (ATP) Large molecules, ions Diffusion – molecules move from area of high concentration to area of low concentration. Osmosis – water moves across membranes (diffuses) to where there is less water. Concentrations affect Osmosis Water moves out of the cell Water moves across membrane (in/out) at equal rates. Water moves into the cell Red Blood Cells in Hypertonic solution (video) Plants and Osmosis Hypertonic solution (video) Concentrations affect osmosis higher amount of solute outside cells equal amount of solute in and out of cells lower amount of solute outside cells Let’s Review Solution is made of solutes dissolved in a liquid or gas. Hypertonic Solution = high amount of solutes Hypotonic Solution = low amount of solutes Isotonic Solution = equal amount of solutes Dynamic Equilibrium is reached when solutes and water move across a membrane at equal rates because concentrations are equal (isotonic). Homeostasis = all living systems need to maintain perfect concentrations inside each cell Test your knowledge On your left side, number 1-14 (short answer) 15 and 16 Extended answers required 1. Which side is hypertonic? A 2. Why? Lower concentration of solute (sugar) B A Higher concentration of sugar B Same concentration of sugar 3. Can the solutes fit across the membrane? 4. How can you describe the membrane since it is very Selectively memselective about what can permeable brane: sugar molecules cannot pass move across? through pores, but Water molecules cluster around sugar molecules water molecules can 5. How can both sides reach equilibrium? More free water molecules (higher concentration) Fewer free water molecules (lower concentration) Osmosis Water moves from an area of higher free water concentration to an area of lower free water concentration ENVIRONMENT 10% NaCL 90% H2O NaCL (salt is ionic, cannot pass through membrane without energy) CELL ? 10% NaCL 90% H2O 6. What is the direction of water movement?_________ 7. Describe the solution outside the cell. ________ 8. Describe the solution inside the cell. __________ 10% NaCL 90% H2O CELL ? 20% NaCL 80% H2O 9. What is the direction of water movement?________ 10. Describe the solution outside the cell. ________ 11.Describe the solution inside the cell. __________ 15% NaCl 85% H2O CELL ? 5% NaCl 95% H2O 12. What is the direction of water movement?_______ 13. Describe the solution outside the cell. ________ 14. Describe the solution inside the cell. __________ 15.How do cell move nutrients, products and wastes in and out of the cell? 16. Why is this traffic across the membrane so important? Demo Lab - - - Materials Celery sticks in different salt solutions??? Iodine solution / starch solution??? Potato cores in different salt solutions??? Procedure Cell membrane tendency of any molecule to spread out into available space Selectively permeable diffusion of a substance across a biological membrane Hypotonic These help transport things across the membrane Hypertonic lower concentration of solutes Isotonic passage of molecules and ions with transport proteins across a membrane down the concentration gradient Passive transport movement of a substance against its concentration gradient with the help of cellular energy Diffusion Membrane only allows certain things across Concentration gradient Barrier made up of phospholipids and proteins that controls what enters and exits the cell Solution Solute Equilibrium/Homeostasis Active transport When there are two different concentrations across a membrane. Molecules always move from high to low concentration without energy (ATP) equal concentrations of solutes the diffusion of water across a selectively permeable membrane Membrane proteins control of water balance Osmosis Combination of a solvent and a solute equally mixed Osmoregulation Molecule used in living systems for energy Facilitated Diffusion ATP State where molecules move across the membrane at equal rates A substance being dissolved in another higher concentration of solutes