Cell Structure & Function http://koning.ecsu.ctstateu.edu/cell/cell.html Cytology-Study of Cell Biology Definition of Cell A cell is the smallest unit that is capable of performing life functions. The Cell • A cell is the smallest unit of living matter. • Don’t confuse this with: atom, element, proton, etc. Cell Structure • Relies on 4 critical properties – Matter – Energy – Organization – Information Matter • Matter found in living matter is composed of elements, molecules, and macromolecules • Life (cells) depend on organic molecules • Cells are composed of the same macromolecules—carbohydrates, lipids, proteins, nucleic acids. • These compounds are composed of 6 main elements. C H O N P S Energy • Needed to carryout life processes – Build molecules and macromolecules(synthesis) – Break down macromolecules and molecules – To carryout cell functions – To overcome disorder , to maintain organization – Energy molecule the cell uses is ATP adenosine triphosphate Organization • Cells are not a haphazard bag of components • Molecules and macromolecules that make up cells have specific sites where they are found. InformationCell organization requires instructions • Genetic materials that carries the instructions • Genome- the entire complement of its genetic material • Every living cell has a copy of its genome • Genome contains the genes that carry the information to create cells with particular structures and functions. Cell Theory • “Theory” is not a casual term in science. • Tested innumerable times with consistent results. • Universally accepted • One of the most basic, fundamental concepts in the foundation of biology. Cell Theory 1800’s 1. Matthias Schleiden- GermanBotanist Studied plant material Saw compartments with dark areas Cells are living entities and plants are aggregates of cells 2. Theodor Schwann-German physiologist friend of Schleiden He saw similar compartments and large numbers nuclei in animal tissues. Schwann extended Schleiden’s hypothesis to animals. 3.Rudolf Virchow-German Biologist Research diseased cells-discovered that “every cell originate from another cell” Cell Theory • All organism are made of cells. • Cells are the smallest structural and functional units of organisms • All cells come from pre-existing cells • Cells are capable of self-reproduction by cell division. Cell Size-Cells are small Types of Organisms based on number of cells • Unicellular organisms – Bacteria, Protists, etc. • Multicellular organisms – Protist – Fungi – Plants – Animals • Muscles, skin, nerves, liver, digestive, bones, blood, immune system, lungs, etc. Two Types of Organism based on Cell Structures •Prokaryotes- organisms that have prokaryotic cells- cells that do not have a nucleus or organelles •Eukaryotes-organism that have eukaryotic cells-cells that have a nucleus and structures called organelles that carryout specific functions. All Cells have the following 1. Plasma membrane-Maintains Homeostasis Structure--Made of 2 layers (bilayer) of phospholipids and embedded proteins, can give some cells their shape Insoluble in water Main Function-Maintain Homeostasis by: Forms a barrier between the cell’s internal environment and the external environment Semipermeable-controls movement of material into and out of cell Communicates with other cell membranes and molecules in the external environment 2. Cytoplasm • Region of the cell that is contained within the plasma membrane • Fluid within the cell that contains water and molecules 3. Ribosomes • Made of RNA molecules Ribonucleic Acid • Function- place where polypeptides and protein are synthesize (produced) • Free Ribosomes are found in cytoplasm of both prokaryotic and eukaryotic cells • Bound Ribosomes are found on rough Endoplasmic reticulum in eukaryotic cells 4.Genetic Material-DNA • Prokaryotic Cells-DNA is found in the central part of cytoplasm called the nucleoid region • Eukaryotic Cells-DNA is found inside the Nucleus DNA in the two cell types is precisely the same kind of DNA, and the genetic code is exactly the same Examples of Cells Amoeba Proteus Plant Stem Bacteria Red Blood Cell Nerve Cell Prokaryotic Cell • Do not organelles-structures surrounded by membranes • Few internal structures • One-celled organisms, • Bacteria http://library.thinkquest.org/C004535/prokaryotic_cells.html Prokaryotic Cells • Small, less complexity • all materials within the cell are relatively close together • Prokaryotic cells have no nucleus. • DNA is circular (it has no ends) and is found in nucleoid region is essentially an imaginary "structure." There is no physical boundary enclosing the nucleoid. Prokaryotic Cells • Prokaryotic ribosomes are small and composed of only three kinds of rRNA and about fifty kinds of protein. • contains no membrane-bound organelles. Basic Prokaryotic Cell Capsule or Slime layer Some bacteria Surrounds the cell wall and plasma membrane Made up of polysaccharides . Function Traps water to keep the bacterium from drying out and to protect it from phagocytosis (engulfing) by larger microorganisms. More Structures Cell Wall• rigid structure outside the plasma membrane. Functions • It supports and protects the plasma membrane. • It is permeable and will allow material to reach the plasma membrane. More Structures • Pili - Many species of bacteria have pili (singular, pilus), small hairlike projections emerging from the outside cell surface. • These outgrowths assist the bacteria in attaching to other cells and surfaces, such as teeth, intestines, and rocks. Without pili, many disease-causing bacteria lose their ability to infect because they're unable to attach to host tissue. • Flagella - Flagella (singular, flagellum) are hairlike structures that provide a means of locomotion for those bacteria that have them. They can be found at either or both ends of a bacterium or all over its surface. The flagella beat in a propeller-like motion to help the bacterium move toward nutrients; away from toxic chemicals; or, in the case of the photosynthetic cyanobacteria; toward the light. Eukaryotic • Contain organelles small structures surrounded by membranes that perform specific functions • All other organisms except Bacteria Plant http://library.thinkquest.org/C004535/eukaryotic_cells.html Animal “Typical” Animal Cell http://web.jjay.cuny.edu/~acarpi/NSC/images/cell.gif “Typical” Plant Cell http://waynesword.palomar.edu/images/plant3.gif Surrounding the Cell Cell Membrane • Outer membrane of cell that controls movement in and out of the cell • Double layer of phospholipids (bilayer) • Animal (Blue #1) • Plant (Blue #4) http://library.thinkquest.org/12413/structures.html Cell Wall • Most commonly found in plant cells & bacteria • Made of carbohydratescellulose in plants • Not found in animal cells • Supports & protects cells • Plant Cell (Red #2.3) http://library.thinkquest.org/12413/structures.html Cells walls are in plant cells not animal cells Inside the Cell Eukaryotic Cell Part I. Cytosol Occupies about 50 % of volume Site of many chemical reactions-Metabolism 1. Main Structure is Cytoskeleton 1.Microfilaments 2. Microtubules 3. Cilia 4. Flagellum Part II Nucleus Nucleus • Directs cell activities • Separated from cytoplasm by nuclear membrane • Contains genetic material - DNA Nucleus: DNA stored here. The Control Center Nuclear envelope: membrane surrounding the nucleus Nuclear pores: open portals of communication between the nucleus & cytoplasm Chromatin: condensed DNA Chromosome: very tightly packed DNA Nucleolus: dense region of chromatin Nucleolus • Inside nucleus • Contains RNA to build proteins http://library.thinkquest.org/12413/structures.html Nuclear Membrane • Surrounds nucleus • Made of two layers • Openings allow material to enter and leave nucleus http://library.thinkquest.org/12413/structures.html Chromosomes • In nucleus • Made of DNA • Contain instructions for traits & characteristics http://library.thinkquest.org/12413/structures.html Part III Endomembrane System • Network of membranes and organelles where molecules are produced and modified and transported in and out of cell Endoplasmic Reticulum • Interconnected highway within the cell • Moves materials around in cell • Smooth type: lacks ribosomes • Rough type (pictured): ribosomes embedded in surface http://library.thinkquest.org/12413/structures.html Ribosomes: protein factories Rough ER: make proteins (studded with ribosomes) Smooth ER: make lipids, modify proteins made in RER Golgi Bodies • Protein processing, modifications and repackaging organelle • Called Golgi complex or apparatus also http://library.thinkquest.org/12413/structures.html 1-receives proteins & lipids in membrane-bound vesicles from ER. 2-modifies those proteins & lipids. 3-sorts and ships the proteins & lipids away in membrane-bound vesicles. Lysosome • Digest cell parts and waste • Contains many digestive enzymes • Break down proteins, fats, and carbohydrates and waste products • Transports undigested material to cell membrane for removal • Cell breaks down and is digested if lysosome membrane is broken • Animal development http://library.thinkquest.org/12413/structures.html Vacuoles • Membrane-bound sacs for storage, • Stores water, waste, toxins, • Stores enzymes for digestion, and waste removal • Contains fluid (water and molecules • Help plants maintain shape (central vacuole) http://library.thinkquest.org/12413/structures.html Central Vacuole-takes up much of plant cell volume Tonoplast is the membrane around the central vacuole Central vacuole stores water, salts, organic compounds etc. for the plant cell The pressure inside the vacuole builds up and enables plant cells to support omplex structure such as leaves and flowers Vacuoles and vesicles are storage organelles in cells. Vacuoles are larger than vesicles. Either structure may store water, waste products, food, and other cellular materials. In plant cells, the vacuole may take up most of the cell's volume. The membrane surrounding the plant cell vacuole is called the tonoplast. Video of Paramecium contractile vacuole http://www.youtube.com/watch?v=4z98WI eNtjM Part IV Semiautonomous Organelles • These organelles can grow and divide to reproduce themselves • But still depend on other parts of the cell Mitochondria • Small, can be few hundred to thousands. • Cell needing a lot of energy will have thousands • Cellular Respiration – changes chemical energy ( fats & carbohydrates) into a energy molecule(ATP) • ATP is what cell used to do work • Controls level of water and other materials in cell • Recycles and decomposes proteins, fats, and carbohydrates http://library.thinkquest.org/12413/structures.html Contains Maternal DNA Chloroplast-site of Photosynthesis • Only found in plant cells and other photosynthetic cells • Never found in animal cells Chloroplast • Contain there own DNA • Contains green chlorophyll pigment • Where photosynthesis takes place • Converting Inorganic compounds into sugar using sun’s energy http://library.thinkquest.org/12413/structures.html Not found in animal cells Peroxisomes • Catalyze certain chemical reactions • Breaks down molecules by removing hydrogen or adding oxygen • Liver cells have many peroxisomes – Toxins in the liver cells are broken down and release hydrogen peroxide- a lethal toxin to cells. – Catalase enzyme breaks hydrogen peroxide into water and oxygen gas