The Basics of Bacteria What are bacteria? Bacteria are single-celled prokaryotes DNA is not located in a nucleus Bacteria appeared on Earth at least 3.5 billion years ago Bacterial Shapes Most bacteria have one of the following shapes: Bacilli Cocci Spirilla Bacterial Structures Most bacteria have the following structures: Cell wall (contains peptidoglycan, a compound composed of amino acids and carbohydrates) Cell membrane Cytoplasm Chromosome (Single-loop of DNA) Additional Bacterial Structures Some bacteria may have the following structures: Capsule (covering outside of cell wall that protects bacterial cell) Pili (hair-like structures used to transfer genetic material from one bacterium to another) Endospore (Thick covering around DNA; developed under harsh conditions) Plasmid (small, additional loop of DNA) Flagella (Used for motion) Nutrition and Growth Bacteria obtain their energy in one of two ways: Heterotrophs (must obtain “food” for energy) Autotrophs (obtain energy from sunlight or minerals) Nutrition and Growth continued Oxygen requirements Obligate anaerobes (can’t survive in presence of oxygen) Facultative anaerobes (don’t require oxygen, but can survive around it) Obligate aerobes (require oxygen) Bacterial Reproduction Bacteria reproduce by binary fission: One cell divides to form two identical cells http://www.yteach.com/index.php/resources/amino_acids_antibiotics_autotroph_protein_cell_wall_chem oautotrophic_chemosyntesis_chlorophyll_saccharides_carbohydrates_cytoplasm_DNA_energy_enzyme_ev olution_fermentation_phospholipids_histone_nucleus_DNA_RNA_population_t_page_37.html Genetic Recombination If bacteria reproduce “clones” by binary fission, why aren’t all bacteria identical? Transformation: cell takes in DNA from environment and replaces a portion of its own DNA with this new DNA Conjugation: A bacterium attaches its pilus to another cell and transfers a copy of its plasmid to this cell Transduction: Virus “infects” a bacterium with a piece of bacterial DNA it is carrying from a different bacterial cell Transformation transformation and conjugation video http://highered.mcgrawhill.com/sites/0072556781/student_view0/chapter13/animation_quiz_ 1.html Conjugation http://www.hhmi.org/biointeractive/animation s/conjugation/conj_frames.htm Transduction http://faculty.ircc.edu/fac ulty/tfischer/images/speci alized%20transduction.jpg http://highered.mcgraw-hill.com/sites/0072556781/student_view0/chapter13/animation_quiz_2.html Bacterial Defenses Bacteria have numerous defenses against the threats in their environment: Exotoxins: Poisonous proteins secreted by bacteria Endotoxins: Toxin in bacterial cell wall; released when bacteria die Naturally-Occurring Antibiotics Antibiotics Chemicals that interfere with cell functions; inhibit bacteria growth. Bacteria are sensitive if they don’t grow in the presence of the antibiotic. Bacteria are resistant if they do grow in the presence of the antibiotic. Bacteria Growth and Antibiotics http://www.sumanasinc.com/scienceinfocus/sif_antibiotics.html