Osmosis, Diffusion, and Cell Transport Biology II Standards 1. Matter tends to be cycled within an ecosystem, while energy is transformed and eventually exits an ecosystem 2. The size and persistence of populations depend on their interactions with each other and on the abiotic factors in an ecosystem 3. Cellular metabolic activities are carried out by biomolecules produced by organisms 4. The energy for life primarily derives from the interrelated processes of photosynthesis and c ellular respiration. Photosynthesis transforms the sun’s light energy into the chemical energy of molecular bonds. Cellular respiration allows cells to util ize chemical energy when these bonds are broken. 5. Cells use the passive and active transport of substances across membranes to maintain relati vely stable intracellular environments 6. Cells, tissues, organs, and organ systems maintain relatively stable internal environments, eve n in the face of changing external environments 7. Physical and behavioral characteristics of an organism are influenced to varying degrees by h eritable genes, many of which encode instructions for the production of proteins 8. Multicellularity makes possible a division of labor at the cellular level through the expression of select genes, but not the entire genome Warm-up (9-9-14) Is it important to learn proper techniques for handling technology within the lab? What are some capabilities technology has given people throughout the last decade? (Think of some capabilities that are science related too!!) Outline for the day • Objectives • LabQuest Demo & exploration Objectives • To explore the LabQuests and learn how to properly use Logger Pro and the new lab technology Warm-up (9-10-14) • Cross off the box for today’s warm-up. We will not be using it. • Please make sure that 4 of you are logged on to the laptops next door. • After you are logged on, come back in this room and be prepared for more instructions. Warm-up (9-11-14) • Explain some of the capabilities of Logger Pro that you learned yesterday that you maybe didn’t know before. Warm-up (9-12-14) • While working with Logger Pro, do you ever need to open up a Word document to type? • What is one of the easiest and quickest ways to get information if you and your group members don’t know how to do something? Outline for the day • Objectives • Work Time Objectives • To explore LabQuest and Logger Pro and to teach the class how to perform various functions that they may not have known before Warm-up (9-15-14) • When using the LabQuests, what are the procedures for turning it on? • When using the LabQuests, what are the procedures for turning it off? • How do you switch between pages in Logger Pro? Outline for the day • Objectives • Work Time Objectives • To explore LabQuest and Logger Pro and to teach the class how to perform various functions that they may not have known before • To present our Logger Pro assignments and learn new functions of Logger Pro. Warm-up (9-16-14) • When using Logger Pro, what is the procedure for making Logger Pro save each run automatically? • If I don’t set the preferences to save automatically, what must I do? Outline • • • • Objectives Present Logger Pro Any final questions Transport Notes Objectives • To present our Logger Pro assignments and learn new functions of Logger Pro. Warm-up (9-17-14) • What does cell transport mean? What does it make you think about? • How is cell transport involved in the transmission of the Ebola virus? Outline for the day • Objectives • Anatomy of an egg • Why do I care? Objectives • To introduce the concepts of cell transport and the different types of transport • To explain the processes of diffusion and osmosis • Determine the types of transport and how a cell maintains a stable internal environment. Anatomy of an Egg • https://www.exploratorium.edu/cooking/eggs /eggcomposition.html Why do I care? • You are made of cells… Without cell transport you would not be alive! • The movement of various solutes can contribute to a variety of bodily functions that occur in your daily lives. – Sodium / Potassium pump Lab Notebook Setup • Number your notebook starting with page 1 in the very front. Number both front and back of the pages. • Composition notebook, the first 2 pages front and back (pages 1-4) will be used for the table of contents • Make sure to label each of these pages. p. 5 Egg Anatomy • Predict: (What do you think is going to happen? What do you notice before?) • Observe: (What do you notice? What is happening? What is the end result?) • Explain: (Why did this happen? What does this show? Why is this important?) p. 6 Strainer Transport • Predict: • Observe: • Explain: Strainer Transport • What does the strainer represent? • What could the substances going through the strainer represent? • Can the material only go one way? • What do you know about the concentrations of the substances on either side of the membrane? Types of Transport • Three types of transport in cells 1. Passive Transport: does not use the cell’s energy in bringing materials in & out of the cell 2. Active Transport: does use the cell’s energy in bringing materials in & out of the cell 3. Bulk Transport: involves the cell making membrane bound vesicles to bring materials in & out of the cell Passive Transport • There are 3 types of passive transport: 1. Diffusion: involves small or uncharged molecules entering & leaving the cell 2. Osmosis: involves water entering & leaving the cell 3. Facilitated Diffusion: involves large or charged molecules that need a protein helper to get in & out of the cell Diffusion Diffusion Diffusion is the netnet movement • Diffusion is the movementofofaa substance substance (liquid oran gas) an area of (liquid or gas) from areafrom of higher higher concentration one ofconcentration. lower concentration to one to of lower concentration. drop of dye in water but is A drop of dye inA water is concentrated concentrated then begins disperse then begins tobut disperse throughtoout the water moving out fromthe anwater area ofmoving high to from an area of low through concentration. an area of high to an area of low concentration. Warm-up (9-18-14) • Why is it necessary to have a separate definition for the diffusion of water? • What is the difference between active and passive transport? Outline for the day • Objectives • Check Egg Experiment • Cell Transport Notes Objectives • To explain the processes of diffusion and osmosis • Determine the types of transport and how a cell maintains a stable internal environment. • Diffusion When the substance has fully Diffusion dispersed through out the container, it has reached When the substance has fully dispersed through equilibrium. out the container, it has reached equilibrium. • What do in you notice about thehow molecules A and Notice the picture below picture? B are evenly distributed through out the • When equilibrium been container. Whenhas equilibrium has been reached, there is no longer a reached, there is no longer a concentration gradient. concentration gradient. A • A concentration gradient is the concentration gradient is the difference in concentration difference concentration between twoinareas. between two areas. Diffusion Certain moleculesDiffusion can freely diffuse across the cell membrane. Look at the picture hydrophobic • belowCertain molecules can molecules and small freely diffusemolecules across the can uncharged cell membrane. diffuse through the • membrane Look at the picture but large hydrophobic molecules molecules or ions and small uncharged (atoms with positive molecules can adiffuse the membrane orthrough negative charge) but large molecules or can not move ions (atoms with a through the positive or negative membrane. charge) can not move through the membrane. Osmosis • Osmosis is the diffusion of water from an area of high concentration to an area of low concentration across a membrane. • Cell membranes are completely permeable to water and the amount of water in the environment has a large effect on the survival of a cell. . Cell membranes are completely permeable d the amount of water in the environment has ect on the survival of a cell. The picture shows arated by ne and how moves from high on to an . Little solute Lots of solute Lots of water Little water The picture shows a tube separated by a membrane and how the water moves from an area of high concentration to an area of low. Osmosis • There are 3 types of solutions that involve water and how they affect the cell. 1. Hypertonic Solution: the solution the cell is placed in has less water than the cell 2. Hypotonic Solution: the solution the cell is placed in has more water then the cell 3. Isotonic Solution: the solution the cell is placed in has equal amount of water as the cell Osmosis are 3 types of ns that involve and how they he cell. They are: ertonic Solution: the n the cell is placed in s water than the cell otonic Solution: the n the cell is placed in ore water then the cell onic Solution: the n the cell is placed in ual amount of water as Hypertonic Solution • Hypertonic solution: higher concentration of water inside the cell than outside the cell. – more solute (salt, sugar, etc.) than the cell • causes there to be less water in the solution. – Water flows from an area of high concentration to an area of low and leaves the cell. • causes the cell to shrivel. Shriveling Cells • Water Loss = • In animal cells, the shriveling is called crenating. • In plant cells, plasmolysis occurs and the cell membrane shrinks away from the cell wall. • Death will result in both cells. has more solute (salt, sugar, etc.) than the cell and this causes there to be less water in the solution. Water flows from an area of high concentration to an area of low and leaves the cell. This loss of water causes the cell to shrivel. In animal cells, the shriveling is called crenating. The red blood cells in the picture to the left have crenated. In plant cells, plasmolysis occurs and the cell membrane shrinks away from the cell wall. Death will result in both cells. Plasmolysis occurring in a plant cell Crenated red blood cells Normal cell Cell in plasmolysis Hypotonic Solution • Hypotonic solution: higher percentage of water than the cell. – less solute than the cell and causes the solution to have more water than the cell. – When a cell is placed in a hypotonic solution, water flows from an area of high concentration to an area of low and rushes into the cell. • causes the cell to expand and possibly burst. Cell Swelling • In animal cells, the cell bursts or will lyse, killing the cell. • In plant cells, the cell membrane is pressed up against the cell wall but the cell wall does not allow the cell to expand anymore and the plant cell does not die. concentration to an area of low and rushes into the cell. This causes the cell to expand and possibly burst. In animal cells, the cell bursts or will lyse, killing the cell. In plant cells, n a hypotonic solution, the solution contains a higher the cell membrane is pressed up against the cell wall but the cell percentage of water than the cell. A hypotonic wall less doessolute not allow expand anymore and the plant cell solution has than the the cell cell to and this causes does not die. the solution to have more water than the cell. When a cell is placed in a hypotonic solution, water flows from an area of high concentration to an area of low and rushes into the cell. This causes the cell to expand and possibly burst. Plant cell in a n animalHypotonic cells, the solution cell bursts or will lyse, killing the cell. In plant cells, the cell membrane is pressed up against the cell wall but the cell Red blood cells beginning to lyse wall does not allow the cell to expand anymore and the plant cell does not die. Hypotonic Solution Plant cell in a Hypotonic solution Red blood cells beginning to lyse Warm-up (9-22-14) • Divide your box in half. On one half label it isotonic, on the other half, label it hypertonic. • Draw a picture of an isotonic solution. • Draw a picture of a hypertonic solution. Outline for the day • • • • Objectives Egg observations Cell Transport with an Egg Simulation Review of hypertonic, isotonic, and hypotonic Objectives • To review hypertonic, hypotonic, and isotonic solutions. Eggsperiment! • Record your observations of the egg in your composition notebooks. • Feel free to touch the egg. Be careful! Review! Hypertonic, Hypotonic, Isotonic • http://www.phschool.com/science/biology_pl ace/biocoach/biomembrane1/solutions.html • Egg Osmosis • https://www.youtube.com/watch?v=SSS3EtKA zYc • Osmosis and water potential (9-23) • http://www.youtube.com/watch?v=nDZud2g1 RVY Cell Transport Review • https://www.youtube.com/watch?v=kfy92hda AH0 Warm-up (9-23-14) • Explain what you learned from the egg demonstration. Outline for the day • Objectives • Notes Scavenger Hunt Objectives • To discover more information about cell transport and the structure of membranes Review! Hypertonic, Hypotonic, Isotonic • http://www.phschool.com/science/biology_pl ace/biocoach/biomembrane1/solutions.html • Egg Osmosis • https://www.youtube.com/watch?v=SSS3EtKA zYc • Osmosis and water potential (9-23) • http://www.youtube.com/watch?v=nDZud2g1 RVY Osmosis • Why is there a separate definition for the diffusion of water?? – Living systems have semipermeable membranes – This may or may not allow the passage of a solute • Remember that water is a solvent! – LAWS OF DIFFUSION WON’T WORK • Solute concentration (diffusion) has to be replaced by water potential. • Living systems create the need for the concept of osmosis – What is water potential? • The sum of the effect of pressure and solute potentials Water Potential • ᴪ (Psi) = ᴪs + ᴪ p • Made of pressure potential and solute potential – Pressure potential: how much water the cell already has – Solute potential: how much solute is dissolved • This influences pressure potential – Water flows from areas of high water potential to low water potential Isotonic Solution • isotonic solution: same percentage of water on the outside of the cell as the inside of the cell. – same amount of solute as the inside of the cell. – Water moves at a constant rate in and out of the cell and the cell maintains its original shape. Isotonic Solution In an isotonicIsotonic solution, there isSolution the same • percentage of water on the outside of the cell as the inside of the cell. An isotonic solution has same amount solute as keeps its In animal andtheplant cells,ofthe cell the inside of the cell. Water moves at a shape when in an out isotonic solution. Most cells In an isotonic there the cell same constant ratesolution, in and ofisthe and the percentage of water on the outside of the cell maintains its original shape. live in cell anasisotonic environment the inside of the cell. An isotonic and they are In animal and has plant cell ofkeeps solution thecells, same the amount soluteits as ableshape tothemaintain their shape and when inthe ancell. isotonic inside of Watersolution. moves at aMost survive. in and outenvironment of the cell andand the cellsconstant live in rate an isotonic original shape. theycell aremaintains able to its maintain their shape and In animal and plant cells, the cell keeps its survive. Isotonic Solution shape when in an isotonic solution. Most cells live in an isotonic environment and they are able to maintain their shape and survive. Plant cells in an isotonic solution Plant cells in an isotonic solution Red blood cell in an isotonic so Red blood cell in an isotonic solution Hypertonic and Hypotonic Solutions Hypertonic and Hypotonic • The plantSolutions cell to the left is placed in distilled water and The plant cell to the left is placed salt solution. distilled water and salt solution. Notice whatwhat happens to the cell in Notice different types of solutions. happens to the cell in the different types of solutions. The red blood cell to the right is placed in Hypertonic and Hypotonic Solutions The plant cell to the left is placed in • The red blood cell to the right is placed in distilled water and salt solution. Notice The red blood cell to the right is placed in distilled waterhappens and salt solution. what to Notice what happens to the cell in the different types in of solutions. the cell the different types of solutions. distilled water and salt solution. Notice what happens to the cell in the different types of solutions. Facilitated Diffusion Facilitated Some molecules are too large to pass Diffusion through the cell membrane by diffusion • • and need help to cross. These molecules use facilitated diffusion. Facilitated diffusion is the flow of large molecules from an area of high an area low using proteins in the cell membrane. Some concentration moleculestoare toooflarge Glucose is able tothe entercell our cells to pass through from the blood stream by membrane bydiffusion. diffusion and facilitated A glucose need help to iscross. molecule too big These to squeeze throughuse the phospholipid molecules facilitated bilayer and needs protein diffusion. channels to help it pass into Facilitated is the the cell.diffusion These protein extremely important flow of“helpers” large are molecules from because theyconcentration allow much needed an area of high molecules to enter our cells. With to an area of our lowcells using out them, would not have proteins in the cellcells would not be glucose and our able to make energy. membrane. Facilitated Diffusion • Glucose is able to enter our cells from the blood stream by facilitated diffusion. A glucose molecule is too big to squeeze through the phospholipid bilayer and needs protein channels to help it pass into the cell. These protein “helpers” are extremely important because they allow much needed molecules to enter our cells. With out them, our cells would not have glucose and our cells would not be able to make energy. Active Transport • The types of transport discussed so far are passive transport and do NOT require a cell to use its energy- the molecules flow with the concentration gradient. There are times when the cell wants to pump against the gradient and to do so, it must use energy. The use of energy to pump molecules against the gradient is called active transport. A cell uses energy in the form of ATP (adenosine tri- phosphate). When energy is taken from ATP, it turns into ADP. Active Transport transport d so far are ransport and do uire a cell to use y- the molecules h the concentration There are times e cell wants to ainst the gradient o so, it must use The use of energy molecules against ent is called ansport. A cell ergy in the form of enosine trite). When energy rom ATP, it turns . The sodium-potassium pump in nerve cells is an example of active transport. Sodium and potassium atoms are pumped against the gradient using ATP. By pumping against the gradient, the cell builds an even bigger gradient (difference between concentrations across the membrane) that helps nerve impulses. Bulk Transport • The last kind of cell transport is bulk transport. • Bulk transport involves the cell membrane making vesicles to bring materials in and out of the cell. There are two kinds of bulk transport: 1. Exocytosis: moving materials OUT of the cell. 2. Endocytosis: moving materials INTO the cell. There are 2 types of endocytosis: 1. Pinocytosis: bringing small molecules or liquids into the cell 2. Phagocytosis: bringing large molecules into the cell Exocytosis • Exocytosis is the process of exporting materials out of the cell by forming a membrane bound vesicle around the materials. The cell uses exocytosis to get rid of cell waste or to export proteins made in the cell to give to other cells. • The proteins or waste are taken to the golgi body where the materials are packaged into a membrane bound vesicle. The vesicle then merges with the cell membrane and the materials are released into the outside environment. uses exocytosis to get rid of cell waste or to export proteins made in the cell to give to other cells. The proteins or waste are taken to the golgi body where the materials are packaged into a membrane bound vesicle. The vesicle then merges with the cell membrane and the materials are released into the outside environment. Micrograph of a vesicle expelling its contents exocytosis Endocytosis and Pinocytosis • Endocytosis is the movement of materials into the cell through membrane bound vesicles. One type of endocytosis is called pinocytosis, or “cell drinking”. • Pinocytosis is the movement of small molecules or liquids into the cell through bulk transport. • The small molecules make contact with the cell membrane and the cell membrane pinches off around the molecules. Pinocytosis is how animal cells make vacuoles (water filled sacs). Endocytosis - Phagocytosis • The other type of endocytosis is phagocytosis, or “cell eating”. Phagocytosis is the movement of large molecules into the cell through bulk transport. • The large molecules make contact with the cell membrane and the cell membrane pinches off around the molecules. The lysosomes then fuse with the vesicle and break down the large molecules into nutrients. Phagocytosis is how white blood cells engulf bacteria and break them down. Phagocytosis is the movement of large molecules into the cell through bulk transport. The large molecules make contact with the cell membrane and the cell membrane pinches off The other type of endocytosis is phagocytosis, or “cell eating”. around the molecules. The lysosomes then Phagocytosis is the movement of large fuse with the vesicle and break down the large molecules into the cell through bulk transport. molecules into nutrients. Phagocytosis is how large molecules make contact with the cell whiteThe blood cells engulf bacteria and break and the cell membrane pinches off themmembrane down. around the molecules. The lysosomes then fuse with the vesicle and break down the large molecules into nutrients. Phagocytosis is how white blood cells engulf bacteria and break them down. Endocytosis-Phagocytosis Micrograph of a white blood cell engulfing virus particles. Micrograph of a white blood cell engulfing virus particles. A cell taking in a food particle and breaking it down. A cell taking in a food particle and breaking it down. Endocytosis Endocytosis A B Above are examples of endocytosis. Determine what type of endocytosis is shown in each situation. Notice the micrograph of actual cells performing the different types of endocytosis. Warm-up (9-24-14) • Explain what crenation is and why this would happen to a cell. (you may need to look back in your notes to find the answer) Outline for the day • Objectives • Cell Membrane Model Objectives • Build a model of a cell membrane and explain the components Cell Membranes • Permeability… – What does this mean? – What is allowed to cross the barrier • Impermeable • Permeable • Semipermeable Why talk about cell transport when we don’t even know what the items are transported through?! Cell Membrane • Now that we know the function, let’s look at the structure to get a better understanding. • http://www.youtube.com/watch?v=S7CJ7xZOj m0 • More Cell Membrane Notes and Information • http://alevelnotes.com/Cell-Membranes/12 Build a Membrane • You will have time to work to build your own model membrane. • I would like these to look really good so I can use them in the future. • Take your time, put in the effort and the work to produce a good final product. • Yes, you may color them! Warm-up (9-25-14) • What is Exocytosis and how is this important to the cell? • What is Endocytosis and how is this important to the cell? Outline for the day • Objectives • Cell Membrane Model Objectives • Build a model of a cell membrane and explain the components Build a Membrane • You will have time to work to build your own model membrane. • I would like these to look really good so I can use them in the future. • Take your time, put in the effort and the work to produce a good final product. • Yes, you may color them! Warm-up (9-26-14) • What is facilitated diffusion and why is it important? Outline for the day • Objectives • Cell Membrane Model Objectives • Build a model of a cell membrane and explain the components Why talk about cell transport when we don’t even know what the items are transported through?! Cell Membrane • Now that we know the function, let’s look at the structure to get a better understanding. • http://www.youtube.com/watch?v=S7CJ7xZOj m0 • More Cell Membrane Notes and Information • http://alevelnotes.com/Cell-Membranes/12 • Osmosis and water potential (9-23) • http://www.youtube.com/watch?v=nDZud2g1 RVY Quiz!! • http://wps.prenhall.com/esm_krogh_biology_ 3/17/4436/1135738.cw/ • Test your progress on understanding active and passive transport. • Report your score to me! Warm-up (9-29-14) • What is diffusion? • What is osmosis? Why is it necessary and what makes it different from diffusion? Outline for the day • Objectives • Lab Prep Objectives • To prepare for the lab that will demonstrate osmosis Lab Prep • Lab 1A and Lab 1B Warm-up (9-30-14) • What is the purpose of the lab 1A? Outline for the day • Objectives • Lab 1A Objectives • To explore the concept of osmosis and diffusion using dialysis tubing Warm-up (10-1-14) • Write down your hypothesis for the lab 1A • Go make sure that your lab stations are set up and the computers and labquests are turned on and ready to go! Outline for the day • Objectives • Lab 1A Objectives • To explore the concept of osmosis and diffusion using dialysis tubing Warm-up (10-2-14) • Explain what you learned from the start of the experiment yesterday • Go make sure that your lab stations are set up and the computers and labquests are turned on and ready to go! Outline for the day • Objectives • Lab 1A wrap up • Lab 1B prep Objectives • To explore the concept of osmosis and diffusion using dialysis tubing • To prepare for the second portion of the lab Warm-up (10-3-14) • Explain how these labs are helpful to understanding cell transport. Outline for the day • Objectives • Lab 1B Objectives • To explore the concept of cell transport and to demonstrate how this works by completing the lab. Warm-up (10-6-14) • What would you have expected the pressure to do in the osmosis lab? (even though we may not have seen it) Outline for the day • Objectives • Lab 1B Objectives • To explore the concept of cell transport and to demonstrate how this works by completing the lab. Warm-up (10-7-14) • In the osmosis lab, what is one reason that the results showed up as they did? Outline for the day • Objectives • Lab 1B Objectives • To explore the concept of cell transport and to demonstrate how this works by completing the lab. Warm-up (10-8-14) • How could the osmosis lab have been performed in order to get more accurate results? • In other words, what should we have done differently in the lab? Outline for the day • Objectives • Lab 1 Review / Follow up Objectives • To explore the concept of cell transport and to demonstrate how this works by completing the lab. Warm-up (10-9-14) • Write down everything you can remember about cell transport and the labs we did in this unit. Outline for the day • Objectives • Test Objectives • To demonstrate knowledge of cell transport by taking the test. Warm-up (10-10-14) • What is special about carbon compounds? • What are the four main carbon compounds? Outline for the day • Objectives • Carbon Compound Review Objectives • To review previous knowledge of carbon compounds and their purposes. 4 Main Carbon Compounds • • • • Proteins Carbohydrates Lipids Nucleic Acids Carbohydrates • What are they? – Molecules composed of carbon, hydrogen, and oxygen – Sugars and starches – Can be broken down to provide a source of usable chemical energy for cells – Major part of plant cell structure – Monosaccharides – most basic • Glucose – Disaccharides – Polysaccharides • Polymers of monosaccharides Cellulose vs. Starches and Glycogen • Cellulose has different bonding of glucose monomers • Starches are made and stored by plants, and can be broken down as source of energy by plant and animal cells • Glycogen – made and stored by animals is more highly branched than plant starches Lipids • Nonpolar molecules • Fats, oils, cholesterol • Contain chains of carbon atoms bonded to oxygen and hydrogen atoms • Some can be broken down for energy • Some are part of cell structure • Fats and oils both contain glycerol bonded to fatty acids – Chains of carbon atoms bonded to hydrogen atoms • 3 fatty acids bonded to glycerol – triglycerides Lipids • Saturated – Most animal fats – Every place that a hydrogen atom can bond to a carbon atom is filled with a hydrogen atom. – All single bonds • Unsaturated – Oils – Fewer hydrogen atoms because there is at least one double bond between carbon atoms – Double bonds make kinks Think about it • Why would the kinks in the unsaturated fats be important in living organisms? • Think about how those molecules fit together. Proteins • Most varied of carbon-based molecules in organisms • Polymer made of monomers (amino acids) – Molecules contain carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur – 20 different amino acids – Our bodies can make 12 – Others come from foods • Meat, beans, nuts Amino Acids in Proteins • Form peptide bonds – Covalent bonds formed between the amino group of one amino acid and the carboxyl group of another amino acid. – Through peptide bonds, amino acids are linked into chains called polypeptides • A protein is one or more polypeptides • Specific sequence of amino acids determines a protein’s structure and function – Alpha helix – Beta pleated sheet Nucleic Acids • Provide detailed instructions to build proteins • Long carbon-based molecules • Polymers made up of nucleotides – Nucleotide is composed of sugar, phosphate group, and nitrogen-containing molecule (base) • Only have one function – work together to make proteins • Two Types 1. DNA = stores the info for putting amino acids together to make proteins 2. RNA = helps to build proteins