Chapter 4 Cellular Processes Cellular Energy Cells Use Energy • Maintain homeostasis • To perform all cellular processes • To make energy-storing molecules When they stop using energy, they are dead Energy Relationships • Energy is a one time commodity – every time it is used some escapes and becomes unusable • More energy is needed to build an energy-storing molecule than is stored in the molecule. How do organisms obtain their food? • Autotrophs – “auto” = self – “troph” = nourishment • Heterotrophs – “hetero” = others Autotrophs • Make their own food – They capture light energy and convert it into sugar – Ex: plants, algae, and some bacteria. Heterotrophs • Depend on other organisms for their energy source – Ex: humans, animals, fungi, and most bacteria. ATP – Adenosine Triphosphate • Most energy sources (fats, carbohydrates) are large and must be broken down into smaller units (sugar – glucose) • ATP stores energy in a usable form for all living organisms • The bonds between the three phosphate groups are unstable high-energy covalent bonds ATP Energy Production • When the bonds are broken, a large amount of energy is released (an exothermic reaction) and is available for use in any cellular function that requires energy (an endothermic reaction). • ATP ADP + P + Energy ATP adenosine triphosphate Adenosine 1 2 Phosphates 3 ATP Production • ADP and P can be reused to form ATP with the proper enzymes and adequate supply of energy • ADP + P + Energy ATP ADP adenosine diphosphate Adenosine 1 2 Phosphates ATP-ADP Cycle 4A – 2 PHOTOSYNTHESIS The process of taking light energy and converting it into stored chemical energy sun The is the source of energy for living things! Photosynthesis Reaction • Reaction converting light energy into stored chemical energy 6 CO2 + 6 H2O + light energy C6H12O6 + 6 O2 (Carbon dioxide) (water) (glucose) (oxygen) • Green plants and algae perform this energy transformation in large enough quantities to provide stored chemical energy for most living organisms Photosynthesis is important because… 1) It converts solar energy into usable chemical energy 2) It produces oxygen Light Absorption • Different wavelengths of visible light are seen by the human eye as different colors. • The color we see is actually the color reflected. Chlorophyll a • Primary catalyst of photosynthesis • Green pigment in the grana of chloroplasts • Becomes activated by light energy Chlorophyll a • Chlorophyll a is a blue green pigment – it reflects the blues and greens and absorbs the reds and violets Chlorophyll b • Is a yellow green pigment – that absorbs some of the same pigments as chlorophyll a as well as some of the blues not absorbed by chlorophyll a and reflects some of the yellow greens that chlorophyll a absorbs Absorption Spectrum The Process of Photosynthesis The Light-Dependent Phase • Requires sunlight and water • Occurs in the grana of the chloroplast • Produces: Oxygen , ATP and NADPH (electron carrier that stores energy for later use) Photosynthesis: The Process Light-Independent Phase • Light is NOT required • Occurs in the stroma of the chloroplast • Also called:“Dark phase,” “synthetic phase,” “Calvin cycle,” “carbon fixation cycle” • Is dependent upon the products of the light phase (ATP and NADPH) and CO2 from the atmosphere Conditions for Photosynthesis • Proper wavelengths of light • Sufficient absorption of carbon dioxide • Proper temperatures • Proper amount of water Chemosynthesis: Other autotrophs • A few bacteria use inorganic chemicals (i.e. ammonia or sulfur) to obtain energy • Ex. Symbiotic bacteria in tubeworms in hydrothermal vents convert chemical energy in sulfur into usable energy Cellular Respiration Cellular Respiration The breakdown of a food substance into usable cellular energy in the form of ATP Summary Kinetic energy (sun) stored chemical energy (C6H12O6) = photosynthesis Summary stored chemical energy (C6H12O6) ready-to-use chemical energy ( ) = cellular respiration Cellular Respiration • Aerobic – Requires oxygen, is the opposite of photosynthesis, combines oxygen with sugar to release energy, carbon dioxide and water • Anaerobic – Does not require oxygen Aerobic Cellular Respiration Aerobic Cellular Respiration C6H12O6 + O2 H2O + CO2 + energy (ATP) The Process of Cellular Respiration • Glycolysis • Citric Acid Cycle (Krebs Cycle) • Hydrogen and Electron Transport System Glycolysis • All types of cellular respiration begin with glycolysis. • Does not require oxygen • Occurs in the cytoplasm Glycolysis • Breakdown of glucose into pyruvic acid, H+, and electrons • 2 net ATP Aerobic Cellular Respiration The products from glycolysis are sent to the mitochondria. Aerobic Cellular Respiration 1. Citric Acid Cycle (Krebs Cycle) = Pyruvic acid is broken down into citric acid. − Pyruvic acid Acetyl CoA − Acetyl CoA Citric acid Aerobic Cellular Respiration 2. Hydrogen and Electron Transport System − Occurs in the cristae of the mitochondria Aerobic Cellular Respiration 2. Hydrogen and Electron Transport System − At the end of the chain, H combines with oxygen to form water. − Oxygen is the rate-limiting factor. Energy Facts • Aerobic Cellular Respiration results in the net gain of 36 ATP molecules. + & eH Citric Acid transport Glycolysis Cycle system Location Cytoplasm Mitochondria Mitochondria (matrix) (cristae) Glucose Pyruvic acid H+; e- Pyruvic Products acid; H+; e- CO2; H+; e- ATP; water Reactants ATP 2 net 2 net 32 Anaerobic Respiration • Breakdown of food (glucose) without oxygen • “Cellular fermentation” 2 Types of Fermentation 1) Alcoholic fermentation – pyruvic acid + NADH alcohol + CO2 + NAD+ Ex: yeast 2) Lactic Acid fermentation – pyruvic acid + NADH lactic acid + NAD+ Ex: produced in your muscles during rapid exercise when the body cannot supply enough oxygen to the tissue Energy Facts • Cellular fermentation supplies no ATP energy beyond glycolysis. Energy Facts • Cellular fermentation supplies no ATP energy beyond glycolysis. • Cellular fermentation results in the net gain of 2 ATP molecules. Cellular Respiration Comparison of Photosynthesis and Cellular Respiration Function Energy Capture Energy release Location Chloroplasts Mitochondria Reactants Carbon dioxide and water Glucose and oxygen Products Carbon dioxide and water Glucose and Oxygen Equations 6CO2 + 6H2O + energy C6H12O6 + 6O2 6O2 + C6H12O6 6CO2 + 6H2O + energy Match the following: ____1. Organisms that make their own food A. Chloroplasts ____2. Site of photosynthesis B. Aneorobic ____3.Process occurs in a mitochondrion C. Aerobic ____4. C6H12O6 D. Glucose ____5. Process does not require oxygen E. ATP ____6. Process requires oxygen F. Kreb’s cycle ____7. Adenosine diphosphate G. Glycolysis ____8. Energy storing molecule H. Energy ____9. The anaerobic process of splitting glucose and forming two molecules of pyruvic acid I. ADP ____10. The ability to do work J. Autotrophs WORD BANK 2 ATP 2 ATP 36 ATP 6 NADH 2 FADH Electron transport chain Mitochondrion Cytoplasm Fermentation Glycolysis Glucose Pyruvate Lactic acid Kreb's Cycle