2009 NMVB Conference
NYU Dept of Cardiothoracic Surgery
NYU Dept of Pathology
Friday December 4th, 2009
Personnel @ NYU
• Dept of Cardiothoracic Surgery:
• Harvey Pass, MD (surgeon, NYU steering committee member & PI)
• Audrey Sorensen, RN (research nurse coordinator)
• Samantha Lincoln (data manager)
• Ryan Harrington (lab technician, thoracic lab)
• Angela Pittman (research project coordinator)
• Dept of Pathology:
• Jonathan Melamed, MD (pathologist, NYU steering committee member)
• Jacinta Small (lab technician, pathology lab)
• Tian He (research coordinator; previously Timothy Jolis)
• Xiangtian Kong (pathologist, pathology review)
2008-2009
• Logistic improvements:
• Streamlined specimen/case-notification process
• Refined tissue procurement process with
response team and procurement kits
• Synchronized local data
with MVB database
• Progression on 5-year plans:
• Frozen TMA
• NYU MVB Paraffin TMA 1
Mesothelioma Accrual
Prospective cases:
Retrospective cases:
Total # of cases:
63
157
220
Retrospective Prospective
Year
Overall Total
Cases
Cases
2007
157
31
188
2008
0
21
21
2009
0
11
11
Total:
220
Meso accrual data (cont’d)
• Emphasis on accruing extrapleural pneumonectomy cases
Year
New
cases
collected
Collection Method
Frozen
tissue
only
Paraffin +
Frozen
tissue
Paraffin
tissue
only
2008
7
0
6
1
2009
9
2
7
0
Tissue Collected (research blocks)
Collection Method
# of Blocks / Samples of
Frozen Tissue
OCT
124
FOIL
130
Paraffin Blocks
288
Progression on 5 yr plans-Frozen TMA
 Frozen TMA
Frozen Array mold kit
Frozen TMA
N=13 pts
mvb22
21
mvb22
21
mvb21
89
mvb22
15
mvb22
19
mvb21
89
mvb22
15
mvb22
19
Normal lung tissue
mvb21
90
mvb21
91
mvb21
97
mvb21
90
mvb21
91
mvb21
97
Tumor tissue
(mesothelioma)
mvb22
17
mvb22
14
mvb21
99
mvb22
17
mvb22
14
mvb21
99
mvb22
03
mvb22
11
mvb22
13
mvb22
03
mvb22
11
mvb22
13
mvb22
12
mvb22
12
MVB #s indicate
distinct patients
Frozen TMA: Equipment & Accessories
Plans for Paraffin TMA
• NYU MVB Paraffin TMA 1
•
Design Phase:
• N= cases/ Block X 4 B
• 4 TMA Blocks (same cases on each
block)
• Control tissues, cell lines, frozento-formalin tissue (adv: standardized
processing)
• Construction Phase: MTA II
• Microtomy: Limited number of sections;
histocollimator
• Storage: In desiccators under nitrogen
atmosphere
• High resolution scan of slide for website
Sample paraffin TMA
Paraffin TMA: Equipment & Accessories
Tissue Micro-Arrayer
Desiccators
Proposed map for Paraffin TMA
Column
→
Row ↓
0
A
Liver
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
1
2
3
4
5
6
7
Cell Lines
Tumor
Control
Liver
8
9
10
11
12
13
Special Thanks to…
Today’s conference attendees
And the entire NMVB team