SCC Cancer Tissue Pathology Core Tissue Microarray (TMA) Request Form Version SCC Cancer Tissue Pathology Core Stanton L. Young Biomedical Research Center (Rm. 1471) 975 NE 10th Street, Oklahoma City, OK 73104 (405)-271-6890 Please email the completed form to: [email protected] For Official Use Date Received Date Completed Account Holder: Date: Requestor: Phone: Email: Personnel Project number (optional): Please briefly explain your request. We understand that your design may be complicated. We will contact you for further details: Approximately how many blocks do you want to use for construction of TMA? What core diameter do you want to use? Do you want duplicates? Yes 0.6 mm 1.0 mm 1.5 mm 2.0 mm No Do you want an additional core stored for molecular biology study? Yes How many slides do you want to get out of your TMA? 40 slides 20 slides No 60 slides Service requested: Paraffin-based TMA Frozen tissue-based TMA Note: Tissue Microarray (TMA) is a powerful tool for cancer research and drug discovery. In the TMA technique, individual tissue cores are obtained with a hollow needle from different formalin-fixed, paraffin embedded tissue blocks and are then inserted in a recipient paraffin block in a precisely spaced, array pattern. Each TMA block can be sectioned using a microtome. All resulting TMA slides have the same tissues in the same coordinate positions. Each microarray block can be cut into ~35 sections. These collections of tissue cores result in a dramatic increase in throughput for in situ examination of gene status and gene expression from archival specimens. Applications commonly employed are immunohistochemistry, conventional staining, in situ Hybridization (ISH), fluorescent in situ hybridization (FISH), RNA or DNA expression analysis, evaluation of various biomarkers, and the TUNEL Assay for apoptosis and other morphological and clinical characterizations of different tissues. Take advantage by saving reagent costs while maintaining uniformity of assays. The development of TMAs requires significant interaction between the constructor and investigator.