SCC Cancer Tissue Pathology Core
Tissue Microarray (TMA) Request Form
Version
SCC Cancer Tissue Pathology Core
Stanton L. Young Biomedical Research Center (Rm. 1471)
975 NE 10th Street, Oklahoma City, OK 73104
(405)-271-6890
Please email the completed form to: muralidharan-jayaraman@ouhsc.edu
For Official Use
Date Received
Date Completed
Account Holder:
Date:
Requestor:
Phone:
Email:
Personnel
Project number (optional):
Please briefly explain your request. We understand that your design may be complicated. We will
contact you for further details:
Approximately how many blocks do you want to use for construction of TMA?
What core diameter do you want to use?
Do you want duplicates?
Yes
0.6 mm
1.0 mm
1.5 mm
2.0 mm
No
Do you want an additional core stored for molecular biology study?
Yes
How many slides do you want to get out of your TMA?
40 slides
20 slides
No
60 slides
Service requested:
Paraffin-based TMA
Frozen tissue-based TMA
Note: Tissue Microarray (TMA) is a powerful tool for cancer research and drug discovery. In the TMA
technique, individual tissue cores are obtained with a hollow needle from different formalin-fixed, paraffin
embedded tissue blocks and are then inserted in a recipient paraffin block in a precisely spaced, array
pattern. Each TMA block can be sectioned using a microtome. All resulting TMA slides have the same
tissues in the same coordinate positions. Each microarray block can be cut into ~35 sections. These
collections of tissue cores result in a dramatic increase in throughput for in situ examination of gene
status and gene expression from archival specimens. Applications commonly employed are
immunohistochemistry, conventional staining, in situ Hybridization (ISH), fluorescent in situ hybridization
(FISH), RNA or DNA expression analysis, evaluation of various biomarkers, and the TUNEL Assay for
apoptosis and other morphological and clinical characterizations of different tissues. Take advantage by
saving reagent costs while maintaining uniformity of assays. The development of TMAs requires
significant interaction between the constructor and investigator.