TRANSLATIONAL RESEARCH/PATHOLOGY SHARED RESOURCE Hallgeir Rui, MD, PhD, Director Tel: (215) 503-9259 Email: Hallgeir.Rui@jefferson.edu Zhijiu Zhong, MD, MS, Facility Manager Tel: (215) 503-8826/ 3858 Fax: 215-503-4305 Email: Zhijiu.Zhong@jefferson.edu 233 S. 10th St.,Room 815/BLSB, Philadelphia, PA 19107 Tel: (215) 503-3858 Monday – Friday, 9:00 AM - 5:00 PM http://www.kimmelcancercenter.org/research/resources/translationalresearch/ Mission, Goals, Capabilities To provide outstanding full range histopathology services complemented by access to unique banked tissue collections and Laser Capture Microdissection of tissues to capture individual cells for KCC members and external users. The Translational Research/Pathology Shared Resource Facility is used by over 100 investigators and has provided key data that support numerous publications and grant applications. The facility provides a full range of histopathological services including sample processing, slide preparation and staining of human and animal tissues or cell samples. Automated processing and tissue stainers provide highly reproducible results and allow co-registration of staining in the facility with analysis carried out in clinical pathology laboratories. Immunohistochemical staining is performed on paraffin sections, frozen tissue or cytologic preparations, utilizing a broad range of optimized commercial antibodies or antibodies provided by the investigator to be optimized. Services Paraffin, Processing/Cassette, Embedding/Block Sectionig of paraffin-embedded or frozen tissues Paraffin/Frozen, Cut DNase & RNasefree slide/Slide Frozen, Embedding or block handling/Block De-Calcification H&E Stain/Deparaffinization/Dehydration/Cou nter Stain/Slide Immunohistochemistry, New antibody optimization/Antibody TMA(Tissue microarray) Construction set up/TMA Block Membrane slide (for LCM)/Slide