Clinical Practice Microbiology
Mrs. Dalia Kamal Eldien
Msc in Microbiology
CLS 417
Instructor: Mrs. Dalia Kamal Eldien Mohammed
MSC in Microbiology
E mail: dkmohammed@inaya.edu.sa
Office hours: Monday 8-10 am
 Students should know the specimens received in the
bacteriology laboratory, their collection techniques,
suitability, and processing.
 Introduction to the safety measures implemented in the
laboratories.
 The culture media preparation and sterilization.
 processing of specimens, isolation/ identification of
organisms, and antibiotic susceptibility testing.
 The diagnostic techniques in the routine Clinical
Immunology, Mycology and Virology laboratories will
also be taught.
What this?
• Microbiology: Microbiology can be defined as the biology of microscopic
organisms, or life too small to be seen with the naked eye.
 Microbiology covers several disciplines, including virology
(study of viruses), bacteriology (study of bacteria),
mycology (study of fungi), and parasitology (study of
parasites).
Clinical microbiology: Is a branch that concerned with the prevention,
diagnosis and treatment of infectious diseases.
In addition, this field of science studies various
clinical applications of microbes for the
improvement of health.
Microbiology scientist
Antonie van Leeuwenhoek
 The "Father of Microbiology, born in Delft, Dutch Republic
 considered to be the first microbiologist.
 He is best known for his work on the improvement of
the microscope first to observe and describe single-celled
organisms
Antonie van Leeuwenhoek
• Louis Pasteur:"French chemist was the founder of microbiological
sciences. discoveries of the principles
of vaccination, microbial fermentation and pasteurization.
• Robert Koch:German physician The founder of modern bacteriology, he is
known for his role in identifying the specific causative agents
of tuberculosis, cholera, and anthrax and for giving
experimental support for the concept of infectious disease.
Louis Pasteur
Robert Koch
First assessment
10 marks
Second assessment
10 marks
quizzes
10 marks
projects
10 marks
Final (practical) exam
20 marks
Final (theory) exam
40 marks
Total
100marks
• Beginning of semester
• 30/8
• First assessment
• 11/10 -22/10
• EID ALADHA vacation
• 18/9 -28/9
IWILL MISS YOU
First
assessment
• Second assessment
• 21/4 -29/4
• Practical exam
• 8/12 -14/12
• Final exam
• 15/12 – 7/1
• Beginning of new semster
• 17/1/2016
Topics to be
covered
Bacteriological culture media
Urine specimen
Blood culture
Stool specimen
General bench specimens
Serological test
Review in diagnosis of viral
infection
Review in diagnosis of fungal infection
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Cossart, P., P. Boquet, S. Normark, and R. Rappuolo. Cellular
Microbiology. ASM Press, Washington, DC, 2000
Vandepitte ,J and Verhaegen J,Engbaek K,Rohner P,Poit P,and C C.
Heuck. .Basic laboratory in Clinical
Bacteriology.WHO,Geneva,2nd Edition 2003
Kaufmann, S. H. E., A. Sher and R. Ahmed. Immunology of
Infectious Diseases. ASM Press, Washington, DC, 2001
Madigan, M. T., Martinko, J. M., and J. Parker. Brock Biology of
Microorganisms. Prentice Hall, Upper Saddle River, NJ, 1997
Mims, C., A. Nash, and J. Stephen. Mims' Pathogenesis of
Infectious Disease. Academic Press, San Diego, CA. 2001