1 CHAPTER I INTRODUCTION A. Background of the Study The Jackfruit (Artocarpusheterophylla) commonly called “Langka or Nangka” is a species of tree in the Artocarpus genus of the mulberry family (Moraceae). It is one of the popular fruit species in the Philippines. This is manifested by its wide distribution and cultivation. In Eastern Visayas production has steadily increased through the years which enable it to ship to other regions and major cities like Cebu and Manila some 100 to 300 fruits approximately two (2) to three (3) tons per week. Every part of the jackfruit tree has its respective uses. The immature fruit is used as a vegetable and feeds while the mature fruit is used for desserts and it is processed into beverages and sweets. Its trunk is used as a lumber for houses and building materials. The leaves of this fruit are used as a medicine, fodder, and as a compost material. Its latex is in the list of ingredients for glue and paste. Its roots are used for handle for farm implements. The seed is used for food, also used in feeds, used for planting materials and for making candies. But none had studied the seeds as a potential coffee. Coffee is a beverage prepared from dried ground beans of Coffeaarabica, an African evergreen; the berries are rich in caffeine, which stimulates the CNS and cardiorespiratory system and results in mild addictive symptoms. 2 The researchers pursued this study because using this study, jackfruit seeds can be made another new and useful product in everyone’s daily life; instead of throwing the seeds away. Why the researchers chose coffee because it has been a common hot drink for everyone; it is almost a part daily routine of man. B. Statement of the Problem This study determines the feasibility of coffee made from Jackfruit seeds. It seeks to answer the following questions: 1) What are the characteristics of the different coffee samples from jackfruit seeds in terms of: 2) a) Color b) Texture c) Aroma d) Taste e) Body? What is the acceptability of the different coffee samples from jackfruit seed? 3) Is there a significant difference between the characteristics and acceptability of the different coffee samples from jackfruit seeds and with that of commercial coffee? 3 C. Objectives of the Study 1) To determine the characteristics of the coffee samples from jack fruit seeds by its color, texture, aroma, taste and body. 2) To determine the acceptability of the coffee samples from jack fruit seeds. 3) To determine whether there is a difference between coffee from jackfruit seeds and commercial coffee in terms of characteristics and acceptability. D. Hypotheses There is no significant difference between the characteristics of the coffee samples from jackfruit seed and commercial coffee. There is no significant difference between the acceptability of the coffee samples from jackfruit seed and commercial coffee. E. Significance of the Study There were many researchers who succeeded in previous studies in utilizing different seeds in making coffee. In this study, the researchers targeted to produce coffee from jackfruit seeds since coffee is a part of almost everyone’s lives. Especially those people whose jobs need them to be awaketo avoid certain errors like those who have night shift jobs and students who are also studying or making their projects late at night. Companies that use jackfruit in manufacturing products can have more profit since they can use the seeds instead of just throwing them away. The country’s waste disposal problems may decrease for the seeds can be used to form new products. 4 F. Scope and Limitations of the Study This study is limited to the feasibility of coffee from jackfruit seeds. Three samples were made with three teaspoon coffee, one teaspoon of sugar per cup of water. Sample A had coffee from jackfruit seeds that were roasted, boiled and ground, Sample B had coffee from jackfruit seeds that were only boiled and ground and Sample C is commercial coffee. It is the scope of the study to compare the characteristics and acceptability of each sample of coffee from jackfruit seeds differently prepared and commercial coffee. The samples were evaluated and rated using Score Card and Hedonic in terms of color, texture, aroma, taste, body and acceptability. A board of evaluators who are coffee drinker were chosen among the students and faculty of MSU-IIT, IDS. The assessment chemical characteristics of the coffee were not included in this study. The coffee from jackfruit seeds were developed at the researchers’ house while the evaluation of the product was done in the vicinity of MSU-IIT CED-IDS for the school year 2011-2012. G. Aroma Definition of Terms A smell, especially the pleasant one. For this study, it refers to the pleasant smell of coffee. Body The viscosity, weight, and thickness of the coffee when in the mouth. 5 Coffee A brewed beverage with a dark, slightly acidic flavor prepared from the roasted seeds of the coffee plant. Grinding To reduce to powder or small fragments by friction. Roasting A cooking method that uses dry heat, whether an open flame, oven, or other heat source. Taste The relation between acidity, aroma, and body gives coffee its taste: caramel, chocolaty, fruity, sweet, piquant, etc. 6 CHAPTER II REVIEW OF RELATED LITERATURE AND STUDIES Jackfruit seeds come from the jack fruit tree; a specie of tree in the Artocarpus genus of the mulberry family (Moraceae). It is native to parts of Southern and Southeast Asia. The tree is well suited to tropical lowlands, and its fruit is the largest tree-born fruit, reaching 80 pounds (36 kg) in weight and up to 36 inches (90 cm) long and (50 cm) in diameter. The flesh of the jackfruit is starchy, fibrous and is a source of dietary fiber. The flavour is similar to a tart banana. Figure 1 Picture of a Jackfruit tree and seeds Varieties of jackfruit are distinguished according to the characteristics of the fruits’ flesh. In Brazil, three varieties are recognized. These are: “hard” variety, which has firm flesh and the largest fruits that can weigh between 15 and 40 kilograms each; “soft” variety, which bears smaller fruits, with softer and sweeter flesh; and the butter 7 variety, which bears sweet fruits whose flesh has a consistency intermediate between the “hard” and “soft” varieties. (Wikipedia, 2011) Jackfruit seeds have around 135 kcal/100 g. They are a rich source of complex carbohydrates, proteins, dietary fiber, vitamins like vitamin A, C and certain B vitamins, and minerals like calcium, zinc, and phosphorus. They also contain lignans, isoflavones, saponins, which are called phytonutrients and their health benefits are wide- ranging from anti-cancer to antihypertensive, anti-ageing, antioxidant, anti-ulcer etc. (FruitsInfo, 2011) In general, the seeds are gathered from ripe fruit, sun dried and stored for use in rainy seasons in many parts of South Indian states. Different variety of recipes prepared in Southern India where they are eaten by roasting as a snack or added to curries in place of lentils. (Wikipedia, 2011) Coffee, a drink with a mildly stimulating effect that is made from the ground or processed seeds of a tropical tree. It contains primarily caffeine which is a stimulant and other substances like proteins and amino acids Alanine, Arginine, Aspartic acid , Cystine, Leucine, Lysine, Phenylalanine, Proline, Glutamic Acid, Glycine, Histidine, Isoleucine, Serine, Threonine, Tyrosine, Valine that are helpful in muscle development and repair. (“Nutrition Facts for Coffee”, 2009) The average caffeine content in coffee varies from the way it is made: brewed 80 to 135 mg, decaf-brewed 3 to 4 mg, decaf-instant 2 to 3 mg, drip coffee 115 to 175 mg, espresso 100 mg, instant 65 to 100 mg. Evidence suggests that caffeine may help fend of Parkinson’s Disease. Scientists at Massachusetts General Hospital in Boston found out that caffeine seemed to protect mice brain cells from depletion of the nerve chemical dopamine – the problem causing 8 Parkinson’s in humans. However these are preliminary findings; human studies have not consistently supported caffeine’s protective role. (Reader’s Digest, 2002) Coffee also has vitamins and minerals that are essential to our body. These vitamins are within the plain, ordinary coffee: Choline, Folate, Niacin, Vitamin K, Pantothenic acid, and Riboflavin. In learning about the nutrition facts for coffee, scientists also discovered that it contains trace amounts of these minerals: Calcium, Fluoride, Magnesium, Manganese, Phosphorus, Potassium, and Sodium. The calories in coffee without additives are 2.4 calories from carbohydrates 1.0, calories from fat 0.4, and calories from protein 1.0. Coffee is also said to be the one of the best sources of antioxidants, even more than red wine or tea. Red wine or tea are two beverages that have long been known for their cancer fighting qualities and purported abilities to reduce the risk of heart disease.(“Nutrition Facts for Coffee”, 2009) In general, coffee has very many health benefits but drinking coffee too much can result into overdosing of caffeine which can cause a racing heart rate, heart palpitations and in some cases, it can even cause seizures. (Reader’s Digest, 2007) Coffee can be evaluated by its texture, taste or flavour, aroma, color and by its body.(Dan, 2011) Some unpublished research on coffee conducted using different kinds of fruit seeds are discussed in the succeeding paragraphs. Banaag and Quidlat (2008) made coffee using durian seeds. The seeds of the durian were dried and were ground after drying. Three samples were prepared: Sample A contained hot water with only 1 tbsp. of the durian coffee per one-half cup of water, 9 Sample B contained hot water with 2 tbsp of the durian coffee per one-half cup of water, and Sample C contained hot water with 3 tbsp. of the durian coffee per one-half cup of water, wherein all of the samples contained also the same amount of creamer and sugar. The three samples were then evaluated by nine 4th year students with the use of a Score Sheet. The coffee under Sample A and B were rated very good while Sample C was only rated good. Results showed that Durian seeds can be utilized into coffee. Bama (2009) made coffee usingMarang seeds. Two samples of Marang seeds coffee with different mixtures of Marang seeds coffee were used to determine its texture, aroma, taste, and color. Sample A contains pure Marang seeds coffee and Sample B contains Marang seeds coffee with commercial coffee. There were 15 panel of testers who evaluated the two samples using the Scorecard Sheet and the Hedonic Scale. Based on the findings of the study, the two samples in terms of aroma, texture and color did not differ significantly. However, the taste and acceptability of the two samples differed significantly. Many researchers have used different fruit seeds like durian seeds and marang seedsin an attempt to produce coffee and succeeded. However, the utilization of jackfruit seeds as a potential coffee is a new study in providing a cheaper coffee and that is also comparable and similar to commercial coffee and or even more delicious and nutritious. 10 CHAPTER III METHODOLOGY A. Research Design The research employs experimental research design to develop jackfruit (Artocarpusheterophylla) seeds as the main ingredient in making a more nutritious and cheaper coffee. Trial and error were done to arrive to an acceptable product. Three samples were prepared with different components and ratio of ingredients. The samples were then evaluated using a Score Card and a Hedonic Scale according to their taste, texture, aroma, color, body and acceptability by thirty coffee drinkers that were picked through purposive random sampling. B. Materials and Equipments Materials commercial coffee measuring cup frying pan teacups gas stove teaspoon jackfruit seeds water 11 C. Experimental Set-Up Table 1. List of Components for the Different Samples of Coffee SAMPLE COMPONENTS A B Jackfruit Coffee (tsp); (boiled and roasted) 3 0 Jackfruit Coffee (tsp); (roasted only) 0 3 Commercial Coffee (tsp) 0 0 Sugar (tsp) 1 1 Hot water (cup) 1 1 D. C 0 0 3 1 1 General Procedure Preparation of Materials The jackfruit seeds were gathered from a ripe jackfruit and placed on a flat surface. It was then washed thoroughly to remove sticky substances. Commercial coffee was purchased from a store. Making the Coffee After drying the seeds two (2) sets of coffee powders were prepared. One set was boiled, ground and roasted. While the other set was sun-dried, ground and roasted. Seeds were boiled in hot water for 15 minutes until seeds are cooked. Seeds were ground in grinding machine in the market. Ground jackfruit seeds were roasted in a frying fan for 10 minutes until colored black. After making the two sets of coffee from jackfruit three (3) samples of coffee were prepared. Sample A contained 3 teaspoons of jackfruit coffee that were boiled and roasted. Sample B contained 3 teaspoons of jackfruit coffee that were air-dried and roasted. Sample C only contained 3 teaspoons of commercial coffee. The amount of sugar and hot water content was constant which is 1 teaspoon per 1 cup respectively. 12 E. Product Development Multiple trials were conducted to achieve a desired product. The research teacher evaluated the product and gave her suggestions for the improvement and enhancement of the product of the research after each trial. The trials ended when an acceptable product was achieved based on the research teacher’s evaluation. F. Sampling Design The researchers used Purposive Random Sampling by identifying individuals who drink coffee. The researchers selected thirty (30) coffee-drinkers that were asked to evaluate the product of the researchers using Score Card for the characteristics and Hedonic Scale for acceptability. The evaluators were also asked to write their comments at the back of the score card. G. Instruments in Data Gathering The instruments used in data gathering were the Score Card and the Hedonic Scale. The researchers used the Score Card for sensory evaluation of the evaluators; while the Hedonic Scale was used for the measuring of acceptance of the evaluators as shown in the tables below. Table 2. Score Card Scale for Characteristics of the coffee from Jackfruit seeds Rating 5 Color Black Texture Coffee like Aroma Pleasant 4 Slightly Black Brown Slightly not Brown Not Brown Slightly Coffee Like Average Slightly not Coffee Like Not Coffee Like Slightly Pleasant Average Slightly not Pleasant Not Pleasant 3 2 1 Taste Very Savory Slightly Savory Average Slightly not Savory Not Savory Body Very Thick Description Excellent Slightly Thick Average Slightly not Thick Not Thick Very Good Good Fair Poor 13 Table 3. Hedonic Scale for the Acceptability RATING GENERAL ACCEPTABILITY 9 Like Extremely 8 Like Very Much 7 Like Moderately 6 Like Slightly 5 Neither Like Nor Dislike 4 Dislike Slightly 3 Dislike Moderately 2 Dislike Very Much 1 Dislike Extremely H. Product Evaluation The chosen coffee-drinkers were given the Score Card and Hedonic Scale to evaluate the difference coffee samples. The evaluators rated the desirable characteristics of each sample product by using numerical rating from 1 – 5, where 5 is the highest and 1 is the lowest. Specific descriptions per characteristics were laid out in Table 2 for easy evaluation and minimize biases. The general acceptability of the different samples of coffee from Jackfruit seeds was evaluated using Hedonic Scale. The evaluators rated the samples from 1 – 9 were 1 corresponds to “dislike extremely” which means least accepted and 9 corresponds to “like extremely” which means the most acceptable product as shown in Table 3. I. Statistical Tools for Data Analysis The data gathered were analyzed and interpreted to get the overall rating of the product. The weighted mean were employed to find the characteristics and acceptability of different samples of coffee from Jackfruit seeds. Kruskal-Wallis H Test was used in order to know if there is a significant difference among samples. 14 Table 4. Score Card Interpretation Guide Mean General Rating Interpretation Description Color Texture Aroma Taste Body Coffee Like Slightly Coffee Like Very Pleasant Very Savory Very Thick Pleasant Slightly Savory Slightly Thick 4.20 5.00 Excellent Black 3.40 4.19 Very good Slightly Black 2.60 3.39 Good Brown Average Average Average Average 1.80 2.59 Satisfactory Slightly Brown Slightly Not Coffee Like Slightly Not Pleasant Slightly Not Savory Slightly Not Thick 1.00 1.79 Poor Not Brown Not Coffee Like Not Pleasant Not Savory Not Thick Table 5. Hedonic Scale Interpretation Guide MEAN RATING GENERAL ACCEPTABILITY 8.12 - 9.00 Like Extremely 7.23 - 8.11 Like Very Much 6.34 - 7.22 5.45 - 6.33 4.56 - 5.44 3.67 - 4.55 2.78 - 3.66 Like Moderately Like Slightly Neither Like Nor Dislike Dislike Slightly Dislike Moderately 1.89 - 2.77 1.00 - 1.88 Dislike Very Much Dislike Extremely 15 Gathering and Drying of Jackfruit Seeds Boiling, Grinding and Roasting of Jackfruit Seeds Air Drying, Grinding and Roasting of Jackfruit Seeds Adding Needed Ingredients and Hot Water Evaluation of Characteristics Figure 2. Procedures in Production of Jackfruit Seeds into Coffee 16 CHAPTER IV RESULTS AND DISCUSSIONS This chapter focuses on the presentations and discussions of data. The study aimed to utilize jackfruit seeds into coffee. Three samples were made which were then rated according to their characteristics: color, texture, aroma, taste and body. The acceptability of the samples was also evaluated. Table 6. Characteristics of the Jackfruit Seeds and Commercial Coffee CHARACTERISTICS SAMPLE A SAMPLE B SAMPLE C Color 4.07 (Slightly Black) 3.00 (Brown) 4.83 (Black) Texture 3.67 (Slightly Coffee Like) 2.80 (Average) 4.27 (Coffee Like) Aroma 3.87 (Slightly Pleasant) 4.33 (Pleasant) Taste 3.80 (Slightly Savory) Body 2.67 (Average) 2.97 (Average) 2.43 (Slightly Not Savory) 2.10 (Slightly Not Thick) 2.77 (Average) 3.40 (Slightly Thick) Table 6 shows the mean ratings of the characteristics of each sample. Sample A (boiled and roasted jackfruit coffee) was rated very good for its color, texture, aroma and taste. And it was rated good for its body. Sample B (roasted only jackfruit coffee) was rated good for its color, texture and aroma. It was rated satisfactory for its taste and 17 body. Sample C (commercial coffee) was rated excellent for its color, texture and aroma. It was rated good for its taste and very good for its body. From the results shown in Table 6, it can be seen that Sample A (boiled and roasted) has better characteristics over Sample B (roasted only). However, its characteristics are not yet better relative to commercial coffee except for the taste where Sample A is better than Sample C probably due to the preparation set by this study which is 3 teaspoon coffee per cup of hot water. Table 7. General Acceptability of Jackfruit seeds and Commercial Coffee SAMPLE A SAMPLE B SAMPLE C 4.73 4.97 6.60 (Neither Like Nor (Neither Like Nor Acceptability (Like Moderately) Dislike) Dislike) Table 7 shows the general acceptability of the different coffee samples. Sample A was rated 6.60 which means that it was liked moderately. Sample B was rated 4.73 which means that it was neither liked nor disliked. Sample C was rated 4.97 which means that it was also neither liked nor disliked. Based on this result, Sample A (boiled and roasted) is more acceptable over Sample B (roasted only) and commercial coffee. Sample A was more acceptable that commercial coffee probably because the panel of tasters preferred coffee that has characteristics similar to Sample A rather than that of commercial coffee for the preparation set by this study, i.e., 3 teaspoon coffee per cup of hot water. 18 Table 8. Difference of Characteristics Among the Three Samples of Coffee CHARACTERISTICS P-VALUE INTERPRETATION There is a significance difference 0.00786 Color the three samples. There is a significance difference 0.0000176 Texture the three samples. There is a significance difference 0.00000132 Aroma the three samples. There is a significance difference .00000987 Taste the three samples. There is a significance difference .0000164 Body the three samples. between between between between between Table 8 shows the statistical difference among the three samples of coffee. It was found out that there was a significant difference in terms of color, texture, aroma, taste and body of the three samples because their p-values are less than the level of significance which is 0.05. This means that Sample A (boiled and roasted), Sample B (roasted only) and Sample C (commercial coffee) has characteristics that differ significantly. Table 9. Difference of Acceptability between the Three Samples of Coffee P-VALUE INTERPRETATION There is a significance difference between .002 Acceptability the three samples. Table 9 shows that the three samples of coffee differ significantly in terms of its general acceptability because the p-value is less than the level of significance at 0.05. Hence, the null hypothesis which states that there is no significant difference between the three samples of coffee in terms of general acceptability is rejected. This means that Sample A (boiled and roasted), Sample B (roasted only) and Sample C (commercial coffee) has acceptability that differ significantly. 19 CHAPTER V CONCLUSION AND RECOMMENDATIONS A. Summary of Findings This study aims to utilize jackfruit seeds into coffee. There were three samples produced, each with components or ingredients. All samples contain 1 teaspoon of sugar per 1 cup of water. Sample A has three teaspoons of jackfruit seeds that were roasted and ground; Sample B has three teaspoons of jackfruit seeds that were boiled and roasted first before they were ground and Sample C has three teaspoons of commercial coffee. The different kinds of coffee were assessed by 30 evaluators who are coffee drinkers using the Score Card and the Hedonic Scale as their guide. After the evaluation KruskalWallis Test was used to determine whether the samples have a difference in their characteristics and general acceptability. Based on the gathered data, the different samples of coffee vary in characteristics such as color, texture, aroma, taste and body. For Sample A (boiled and roasted), the mean values of its color, texture, aroma, taste and body are 4.07, 3.67, 3.87, 3.80, 2.67 and 3.67, respectively. For Sample B (roasted only), the mean values of its color, texture, aroma, taste and body are 3.00, 2.80, 2.97, 2.43, 2.10 and 2.80, respectively. While Sample C (commercial coffee), the mean values of its color, texture, aroma, taste and body are 4.83, 4.27, 4.33, 2.27, 3.40 and 4.27, respectively. As calculated, Sample C has 20 best result in terms of characteristics since it is commercial coffee. It can be observed, however, that Sample A (boiled and roasted), has comparable mean values of characteristics to Sample C. Hence, it can be a potential coffee. The mean values of acceptability for Samples A, B and C are 6.60, 4.73 and 4.97, respectively. As seen, Sample A (boiled and roasted) is more acceptable over Sample C (commercial coffee) since the panel of evaluators prefers the taste of Sample A than Sample C, probably due to the preparation set by this study which is 3 teaspoons coffee per cup hot water. B. Conclusion Based on the result of the experiment, the following conclusions are made: 1) Coffee can be made from jackfruit seeds especially when it is boiled then roasted. 2) Sample A (boiled and roasted) is slightly black, slightly coffee like, slightly pleasant, slightly savory and its body is average, Sample B (roasted only) is brown, has an average texture and aroma, is slightly savory and slightly not thick and Sample C (commercial coffee) is black, coffee like, has a pleasant aroma, has an average taste and is slightly thick. 3) Sample A is liked moderately, while Sample B and Sample C are neither liked nor disliked. 4) There a significant difference between the characteristics and acceptability of the different coffee samples from jackfruit seeds and with that of commercial coffee. 21 C. Recommendations The researchers recommend the following to help future researchers who want to pursue further studies related to this: 1) To use different kinds of seeds in making coffee. 2) Add more ingredients to the coffee like creamer. 3) Try different methods in making coffee. 4) Try using the jackfruit seeds into a different product like coffee creamer. 22 REFERENCES Encyclopedia Erbe, L. (1993). Jack Tree. Encyclopedia Americana (Volume 15, p.640). Danbury CT Grolier Incorporated Struning, W.C. (1993). Coffee.Encyclopedia Americana (Volume 7, pp.187-191). Danbury, CT. Grolier Incorporated Research Papers Bama, F.A.S. (2009) “Utilization of Marang Seeds into Coffee” A Research Paper, IDS, MSU-IIT Chiu, K.T. & Padilla, J.R. (2010) “Comparative Study Between Coffee From Polished Rice and Coffee From Unpolished Rice” A Research Paper, IDS, MSU-IIT Quidlat, E.D., Jr. (2008) “Utilization of Durian Seeds into Coffee” A Research Paper, IDS, MSU-IIT Magazines Katharine Mieszkowski. 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