PROFESSOR DR. SAMINA KAUSAR

Q.1:
Differentiate between the following: a) Competitive and Non competitive inhibitors. b) Iso-enzymes and Coenzymes.
Supplementary 2005

Q.2: a)
Enumerate the four basic principles involved in
Enzyme classification.
b)
Illustrate the role of Hydrolases with examples from human body.
Supplementary 2006

Q.3: a) What do you mean by: i) Coenzyme ii) Isoenzyme b) Give diagnostic importance of
Alkaline Phosphatase.
Annual 2006

Q.4: a)
Define Enzymes.
b)
How do the following factors affect the rate of an enzyme catalyzed biochemical reaction: i) pH ii) Temperature iii) Substrate concentration iv) Competitive inhibitors
Annual 2007

Q.5: a)
What are Isoenzymes (Isozymes)? How do they help in clinical diagnosis of myocardial infarction?
b) Define Allosteric enzymes, Km value of enzyme.
Annual 2007

Q.6:
Clearly differentiate between the following terms: a)
Apoenzyme and Holoenzyme.
b)
Hydrolases and Lyases: c)
Competitive and Non-competitive inhibitors
Annual 2008

Q.7:
Name the three enzymes having diagnostic importance in the diseases of liver and heart?
Supplementary 2008

Q.8: a) Give diagnostic importance of following enzymes: i) Alkaline phosphates ii) ALT iii) Amylase b) How does covalent modification of enzyme molecule influence the enzyme activity?
Supplementary 2008

Q.9:
a) How can you differentiate competitive inhibitors from the non-competitive inhibition of enzyme activity?
b) Enumerate two clinically important enzyme inhibitors along with examples of enzymes inhibited by them
Supplementary 2008

Q.10: a) Enumerate the factors that modulate the enzyme assisted reactions ?
b)
Draw & Elaborate the graphic representation of enzyme catalyzed reaction on the basis of Michaels
Menton equation & Line weaver burk plot.
Annual 2009

Q.11:
Enumerate 6 factors which can influence enzyme activity. What is the effect of substrate concentration and pH on the rate of enzyme –catalyzed reaction?
Annual 2010

Q.12:
A 60 years old female with a history of breast carcinoma treated by mastectomy three years ago, is now complaining of general malaise and bone pain. Laboratory investigations including LFTs
(test to assess liver function) suggest metastasis (spread) of cancer to liver. a) i) The activities of which plasma enzymes are most likely to be raised in this patient?
ii) Why are the activities plasma enzymes raised in this disease?
b) What do you understand by competitive and noncompetitive inhibition of enzyme activity with example? How does temperature influence the rate of enzyme catalyzed reaction?
Annual 2010

Q.13:
Enumerate the Four important factors affecting enzyme activity.
How is enzyme activity regulated by covalent modification of enzyme molecule along with example ?
Annual 2011

Q.14:
How does pH influences the enzyme activity? Give examples of two enzyme inhibitors used as drug.
How is enzyme activity regulated allosterically?
Supplementary 2011

Q.15: a) Discuss the factors effecting enzyme catalyzed reactions.
b) Explain the phenomenon of enzyme inhibition.
Annual 2012

Q.16: a) How Does enzyme catalysis biochemical reactions?
b) What model of enzyme catalysis in used in biochemical reactions?
Annual 2013

Q.17: a) Enumerate all mechanisms through which enzyme cause catalysis.
b) Give the diagnostic importance of plasma enzymes of differentiate different types of jaundice.
Annual 2013

Q.18: a) What are Isoenzymes? How do they help in clinical diagnosis of myocardial infarction?
b) Differentiate between competitive and non-competitive inhibition.
Annual 2014