Syllabus BCHM 101a, Fall 2015:
Module 1: Kinetics
August 27
August 31
Sept. 2
Sept. 3
Sept 7
Sept. 9
Sept. 10
Sept 14
Sept. 16
Sept. 17
Sept. 21
Sept 23
Sept. 24
Sept 28
Introduction in the World of Enzymes
Review chemical kinetics, first order, second order, reversible reactions,
chemical kinetics, parallel reactions, steady state, molecularity versus order
Transition state theory, temperature dependence of reactions,
no Brandeis classes, Labor Day
Eyring Equation
Energy diagrams for uncatalyzed versus catalyzed reactions, intermediates
versus transition states
no class
Enzyme kinetics, steady state, Michaelis Menten,
Vmax, kcat, rate equations for different enzymatic reactions, Haldane
Relation
General mechanisms of enzyme catalysis
no class
General mechanisms of enzyme catalysis
no class
Module 2: Isomerizations
Sept. 29
Sept 30
Oct. 1
Oct. 5
Oct. 7
Oct. 8
Oct. 12
Isomerizations: [1,1] hydrogen shifts, Proline Racemase
Isotope Effects
Prolyl cis/trans isomerases
no class
[1,2] hydrogen shifts, Triose phosphate isomerase
Triose phosphate isomerase
Exam
Module 3: ThDP reactions
Oct. 14
Oct. 15
Oct. 19
Oct. 20
Decarboxylation: a-ketoacids versus b-ketoacids
no class, Biochem retreat
ThDP reactions, oxidative versus nonoxydative decarboxylation
guest speaker, the renaissance of enzymology in pharmaceutical industry,
how to make drugs
Module 4: Group transfer reactions
Oct. 22
Oct. 26
Oct 28
Oct 29
Nov. 2
Nov. 4
Nov. 5
Nov. 9
Nov. 11
Nov. 12
PDC
Group transfer reactions: Proteases
Chymotrypsin
Chymotrypsin
Inhibitors
Economics of enzyme catalysis
Phosphoryl transfer reactions
Phosphoryl transfer reactions
Kinetics of 2 substrate reactions
Acyl Activation
Module 5: PLP reactions
Nov. 16
Nov. 18
Nov. 19
EXAM
PLP reactions
PLP reactions
Nov. 23
Nov. 30
no class (makeup)
PLP reactions
Module 6: Redox reactions
Dec 1
Dec. 2
Dec. 3
Dec. 7
Dec. 9
makeup: Redox reactions: NAD
DHFR
DHFR
Flavin reactions
Summary of the course
Dec. 11 or 14 ?? Final Exam
Four-Credit Course (with three hours of class-time per week)
Success in this 4 credit hour course is based on the expectation
that students will spend a minimum of 9 hours of study time per
week in preparation for class (readings, papers, discussion
sections, preparation for exams, etc.).