OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which
you are a designated OIE Reference
Laboratory:
Brucellosis
Address of laboratory
Brucellosis Laboratory
Bacterial disease Division, Animal, Plant and Fisheries
Quarantine and Inspection Agency (QIA), Ministry of Food,
Agriculture, Forestry and Fisheries (MIFAFF)
175 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, 430-757,
Republic of Korea
Tel.:
+82-31-467-1765
Fax:
+82-31-467-1778
e-mail address:
brujung@korea.kr
website:
http://www.qia.go.kr
Name (including Title) of Head of
Laboratory (Responsible Official):
Dr. Yong Ho Park DVM, MS, PhD
Name (including Title and Position)
of OIE Reference Expert:
Suk Chan Jung
Date of submission to the OIE
January 2013
QIA Commissioner
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January
through December of the past year (2012), unless otherwise stated, and must be submitted by the end of
January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE
should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference
Laboratories, available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute
with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference
Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Bovine Brucellosis » – « Suk Chan Jung » – « Republic of Korea »
ToR: To use, promote and disseminate diagnostic methods validated according to OIE
Standards
Test recommended by the OIE
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
RBT
1,475
SAT
297
ELISA
332
FPA
168
Dip-stick
61
Direct diagnostic tests
Nationally
Internationally
PCR
88
32
MLVA
13
32
Bacterial culture
88
32
ToR: To develop reference material in accordance with OIE requirements, and implement
and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any
other reagents used in the diagnosis and control of the designated pathogens or
disease.
2.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
2
Type of reagent
available
Related
diagnostic
test
RBT antigen
No
Name of
recipient OIE
Member
Countries and
of institutions
Produced/
stored
Amount supplied
nationally
(ml, mg)
Amount supplied
internationally
(ml, mg)
RBT
Produced
1,000 test
1,000 test
Mongolia,
Vietnam
SAT antigen
SAT
Produced
69,680 test
CFT
CFT
Produced
1,000 test
PCR test kit
(Multiplex PCR)
PCR
Produced
4,800test
480 test
Mongolia
Institute of
Veterinary
Medicine
Annual reports of OIE Reference Centres, 2012
OIE RL for « Bovine Brucellosis » – « Suk Chan Jung » – « Republic of Korea »
4.
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
5.
No
Did your laboratory produce vaccines?
Yes
6.
No
Did your laboratory supply vaccines to OIE Member Countries?
Yes
No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for
diagnosis and control of the designated pathogens or diseases
7.
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
No
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and
technical advice on disease control measures to OIE Member Countries
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
No
Name of OIE
Member Country
seeking assistance
Date
(dd/mm)
Mongolia
06/04
20
17/09
20
10.
No. samples received for
provision of diagnostic support
(i.e. from surveillance campaign)
No. samples received for
provision of confirmatory
diagnoses
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
No
Name of the OIE Member Country
receiving a technical consultancy
Mongolia
Purpose
Methods for producing Brucella
selective medium and multiplex PCR
How the advice was
provided
In loco
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with
other laboratories, centres or organisations
11.
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Annual reports of OIE Reference Laboratories, 2012
3
OIE RL for « Bovine Brucellosis » – « Suk Chan Jung » – « Republic of Korea »
Yes
No
Title of the study
Duration
Purpose of the study
Distribution of Brucella
species from a variety of
animals and the molecular
epidemiological analysis
by MLVA assay in
Mongolia
3 years
Identifying Brucellosis
in Mongolia and
epidemiological
correlation between
Brucella isolates
Partners
(Institutions)
Institute of
Veterinary
Medicine
OIE Member Countries
involved other than
your country
Mongolia
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to
the designated pathogens or diseases
12.
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 9
b)
International conferences: .............................................. 7
c)
National conferences: ...................................................... 4
d)
Other: ...............................................................................
a) Articles published in peer-reviewed journals
Kyung Yuk Ko, Jong-Wan Kim, Moon Her, Sung-Il Kang, Suk Chan Jung, Dong Hee Cho, Ji-Yeon Kim. (2012).
Immunogenic proteins of B. abortus to minimize cross reactions in brucellosis diagnosis. Vet Microbiol,
156:374-380
Eun-Jeong Heo, Sung-Il Kang, Jong-Wan Kim, Moon Her, Donghee Cho, Yun-Sang Cho, In-Yeong Hwang, JinSan Moon, Sung-Hwan Wee, Suk-Chan Jung, and Hyang-Mi Nam. (2012). In vitro activities of antimicrobials
against B. abortus isolates from cattle in Korea during 1998-2006. J Microbiol Biotechnol 22(4):567-570
Jong-So Kim, Wooseog Jeong, Hye-Young Jeoung, Jae-Young Song, Hyungtae Kim, Jeong-Hun Beak, Vinuselvi
Parisutham, Sung Kuk Lee, Jong Wan Kim, Ji-Yeon Kim, Suk Chan Jung, Moon Her and Dong-Jun An. (2012).
Complete genome sequence of B. canis strain HSK A52141 isolated from the blood of an infected dog. J
Bacteriology 194(19):5134
Hyungtae Kim, Wooseog Jeong, Hye-Young Jeoung, Jae-Young Song, Jong-So Kim, Jeong-Hun Beak, Vinuselvi
Parisutham, Sung Kuk Lee, Jong Wan Kim, Ji-Yeon Kim, Suk Chan Jung, Moon Her and Dong-Jun An. (2012).
4
Annual reports of OIE Reference Centres, 2012
OIE RL for « Bovine Brucellosis » – « Suk Chan Jung » – « Republic of Korea »
Complete genome sequence of B. abortus A13334, a new strain isolated from the fetal gastric fluid of dairy
cattle. J Bacteriology 194(19):5444
Md. Siddiqur Rahman, Moon Her, Ji-yeon Kim, Sung-il Kang, Kichan Lee, Muhammad Jasim Uddin, Amitavo
Chakrabartty and Suk Chan Jung. (2012). Brucellosis among ruminants in some districts of Bangladesh using
four conventional serological assays. African Journal of Microbiology Research, 6(22):4775-4781
Hyang-Keun Lee, Jong-Wan Kim, Yun-Mi Ha, Moon Her, Ji-Yeon Kim, Kichan Lee, Sung-Il Kang, Suk-Chan Jung,
(2012). Dignosis of canine brucellosis using recombinant ribosomal protein L7/L12. Korean J Vet Res 52(1):2531
You-Chan Bae, Gye-Hyeong Woo, Ha-Young Kim, Moon Her, Dong-Hee Cho, Suk-Chan Jung, Jae-Ku Oem, JaeWon Byun, Yi-Seok Joo, O-Soo Lee, Myoung-Heon Lee. (2012) Pathological study of Korean native pregnant
cows and fetuses experimentally vaccinated with Brucella abortus RB51 in heifers and challenged with
Brucella abortus 2308 in pregnancy. Korean J. Vet. Publ. Hlth, 36(3):109-113
Ji-Yeon Kim, Shin Seok Kang, Moon Her, Kichan Lee, So-Ra Sung, Jung Hui Gu, Sung-Il Kang, Hyang-Keun Lee,
Yu Jin Kim, Dong Gil Kim, Suk Chan Jung. (2012). Investigation of occurrence factors on brucellosis-outbreak
farm in Chung-buk province, Korea. Korean J Vet Serv, 35(4):263-268
So-Ra Sung, Ji-Yeon Kim, Moon Her, Kichan Lee, Jeong Hui Gu, Sung-Il Kang, Hyang Keun Lee, Suk Mi Kim, SukChan Jung. (2012). Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine
brucellosis. Korean J Vet Ser, 35(4): 269-273
b) International conferences
Ji-Yeon Kim, So-Ra Sung, Jeong Hui Gu, Moon Her, Kichan Lee, Sung-Il Kang, Hyang-Keun Lee, Yu Jin Kim, and
Suk-Chan Jung. (2012). Detection of immunogenic proteins of Brucella abortus RB51 strain by twodimensional electrophoresis (65th Brucellosis meeting in Chicago)
Dong-Gil Kim, Yu Jin Kim, Moon Her, Sung-Il Kang, Kichan Lee, Ji-Yeon Kim, So-Ra Sung, Hyang Keun Lee, Suk
Chan Jung. (2012). Rapid detection of Brucella canis by the loop-mediated isothermal amplification method
(65th Brucellosis meeting in Chicago)
Kichan Lee, Moon Her, Ji-Yeon Kim, Sung-Il Kang, Erdenebaatar Janchivdorj, Suk Chan Jung. (2012). Isolation
of Brucella species from aborted foetuses of sheep and goats in Mongolia (93rd CRWAD in Chicago)
So-Ra Sung, Ji-Yeon Kim, Moon Her, Kichan Lee, Jeong Hui Gu, Sung-Il Kang, Hyang Keun Lee, Suk Chan Jung.
(2012). Investigation on the diagnostic efficiency of the standard tube agglutination test for brucellosis (93 rd
CRWAD in Chicago)
Hyang-Keun Lee, Jong-Wan Kim, Kichan Lee, Donggil Kim, Sung-Il Kang, So-Ra Sung, Yu Jin Kim, Ji-Yeon Kim,
Moon Her, Suk-Chan Jung. (2012). Combination DNA plus Brucella canis vaccine (93rd CRWAD in Chicago)
Annual reports of OIE Reference Laboratories, 2012
5
OIE RL for « Bovine Brucellosis » – « Suk Chan Jung » – « Republic of Korea »
Sung-Il Kang, Moon Her, Ji-Yeon Kim, Kichan Lee, Yun-Mi Ha, Myeong Ju Chae, Hyang Keun Lee, Suk Chan
Jung. (2012). Specific detection of B. abortus by the loop-mediated isothermal amplification (LAMP) assay
(27th WBC, Portugal)
Suk Chan Jung (2012). National Surveillance and Control Measures of Brucellosis in South Korea (NIAH
conference, Thailand)
c) National Conferences
Ji-Yeon Kim, Moon Her, Sung-Il Kang, Kichan Lee, Hyang Keun Lee, Suk Chan Jung. (2012). Molecular typing
assays for epidemiological relatedness between Brucella abortus isolates from cattle and Chinese water deer
(Spring, KSVS)
Kichan Lee, Moon Her, Ji-Yeon Kim, Sung-Il Kang, Hyang Keun Lee, Suk Chan Jung. (2012). MLVA assay as a
tool for the epidemiological survey of Brucella canis in Korea (Spring, KSVS)
Sung-Il Kang, Moon Her, Yu Jin Kim, Ji-Yeon Kim, Kichan Lee, Hyang Keun Lee, So-Ra Sung, A-Ran Yoon, Suk
Chan Jung. (2012). Rapid diagnosis of Brucella abortus using loop-mediated isothermal amplification assay
(Spring, KSVS)
Hyang-Keun Lee, Jong-Wan Kim, Yun-Mi Ha, Kichan Lee, Sung-Il Kang, Ji-Yeon Kim, Moon Her, Suk Chan Jung.
(2012). DNA prime-protein boost vaccine strategy with DNA vaccine coding rL7/L12 protein (Spring, KSVS)
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
6
No
a)
Technical visits: ................................................................ 1
b)
Seminars: .........................................................................
c)
Hands-on training courses: .............................................. 20
d)
Internships (>1 month): ...................................................
Type of technical training
provided (a, b, c or d)
Country of origin of the expert(s)
provided with training
No. participants from the
corresponding country
c
Cambodia
2
c
Indonesia
2
c
Lao
2
c
Malaysia
2
c
Mongolia
2
Annual reports of OIE Reference Centres, 2012
OIE RL for « Bovine Brucellosis » – « Suk Chan Jung » – « Republic of Korea »
c
Myanmar
2
c
Nepal
1
c
Philippines
2
c
Singapore
1
c
Thailand
2
c
Vietnam
2
a
Mongolia
5
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the
pathogen and the disease concerned
16.
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
No
Quality management system adopted
ISO/IEC 17025
17.
Is your laboratory accredited by an international accreditation body?
Yes
No
Test for which your laboratory is accredited
Accreditation body
MRT, RBT, SAT, ELISA, FPA, PCR, MLVA and
Bacterial culture
ilac-MRA
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or
Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes
No
ToR: To organise and participate in scientific meetings on behalf of the OIE
19.
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
20.
No
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
Title of event
Thailand-Japan joint
conference on
animal health 2012
No
Date
(mm/yy)
Location
Role (speaker, presenting
poster, short
communications)
Title of the work
presented
05/12
Thailand
Speaker
National surveillance and
control measures of
brucellosis in South Korea
Annual reports of OIE Reference Laboratories, 2012
7
OIE RL for « Bovine Brucellosis » – « Suk Chan Jung » – « Republic of Korea »
ToR: To establish and maintain a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organise regular inter-laboratory
proficiency testing to ensure comparability of results
21.
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
No
ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results.
24.
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
No
Purpose for inter-laboratory test comparisons1
No. participating
laboratories
Participating OIE Member
Countries
Evaluating multiplex PCR method (specify:
differentiating Brucella species)
1
Mongolia
ToR: To place expert consultants at the disposal of the OIE
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
1
8
No
See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Annual reports of OIE Reference Centres, 2012