BLENDED MICROBIOLOGY SYLLABUS
Tulsa Community College, Southeast Campus
Spring 2013
BIOL_2164_310_21539_201320
Microbiology Section 310
Lecture Online
Lab meets once a week at the TCC Southeast campus
Lab: SE 8204 Friday 11:00 AM - 1:50PM
Hours of Credit: 4 semester hours
Science and Mathematics Division SE 8125
Office Hours: 8:00 AM - 5:00 PM
Associate Dean: Lyn Kent
Phone: 918-595-7742
Instructor: Marva Volk
Office Room Number: SE 8281
Office Hours: T & R 11:00 PM - 4:00 PM
NOTE: I am usually in the Prep room next to lab
SE 8204 if I am not in my office
Phone: 595-7687 Office (try this one first)
918-595-7958 Prep room (no messages)
Email: marva_volk@mail.tulsacc.edu
COURSE PREREQUISITES
BIO1224 (Intro to Biology for Majors) or equivalent* AND CHE1114 (Principles of Chemistry) or CHE1315
(General Chemistry). This course will be taught with the assumption that you have a solid grasp of basic
chemical and biological concepts and terminology. *With approval of SEC Associate Dean of Math and Science.
GENERAL EDUCATION GOAL STATEMENT
The General Education Goals are designed to ensure that graduates of Tulsa Community College have the skills,
knowledge, and attitudes to carry them successfully through their work and their personal lives. General
Education Goals relevant to this course include Critical Thinking, Effective Communication, Engaged Learning,
and Technical Proficiency.
COURSE DESCRIPTION
Included will be a survey of the viruses, protozoa and fungi of medical significance, with an in-depth survey of
bacteria, their morphology, metabolism, pathogenic capabilities and classification. Laboratory will emphasize
those techniques concerned with isolation and pure culture of microorganisms and the use of biochemical criteria
in taxonomy. Lecture 3 hours (online.) Laboratory 3 hours (on campus.)
COURSE OBJECTIVES
 Describe the anatomy and physiology of microorganisms, with emphasis on the bacteria.
 Identify scientists that have made a major contribution to the field of Microbiology and discuss the impact
of their work.
 Compare the modes of reproduction of microbes and contrast the mechanisms of genetic transfer and
gene expression in microbes.
 Discuss the genetic processes and the procedures used in Biotechnology
 Compare the physical and chemical techniques for microbial control, including sterilization, disinfection,
and antimicrobial therapy.
 Examine bacterial diversity in the major orders of bacteria and illustrate the use of morphological and
physiological differences in establishing taxonomic schemes.
 Discuss the concept of pathogenesis and the resultant immune response in the infected host.
 In laboratory, demonstrate proficiency in culturing and staining bacteria and other microbes. Interpret
biochemical test and selective and differential media results. Verify the identity of a bacterial specimen
based on staining, biochemical tests and selective and differential media results
Blended Microbiology Syllabus
page
1
TEXTBOOKS AND OTHER MATERIALS (See Blackboard for ISBN numbers)
REQUIRED: Microbiology, 3rd Edition, Robert W. Bauman, Benjamin Cummings Publishing Co.
A Photographic Atlas for the Microbiology Laboratory 4th Edition, Leboffe and Peirce. Morton Publishing
Lab Exercises for Microbiology 2164, TCC Southeast Campus,* Marva Volk, Neil Enis, Dusti Sloan, Ivan Lurz;
Revised January 2013, TCC Publishing (Includes printed copies of Blackboard quizzes.)
Labcoat* (disposable), safety glasses (sunglasses style)*, slides (1 package)*, non-latex gloves, colored pencils,
permanent marker (fine tip), large 3 ring binder for lab notebook and handouts. Coverslips will be provided.
*Best to purchase these items from the Southeast Campus bookstore.
Required software: Microsoft Office (Word, Excel, PowerPoint). If you do not have a current version of
Microsoft Office, you can download one free from Blackboard. Go to the Organization tab (3rd tab from the left),
use the Organization Search box to search for: software. Enroll in the SoftwareDownloads organization and
follow the on-screen instructions.
Required email address: All TCC students receive a designated “TCC Outlook” email address (ex:
jane.doe@tulsacc.edu). All communications to you about this course and TCC will be sent to your TCC Outlook
email address. You must use TCC Outlook email to receive email from, and send email to, your instructor
regarding this course. You may access TCC Outlook from the Blackboard homepage or from
http://www.outlook.com/tulsacc.edu
SUPPLEMENTAL: AIDS Update 2013, Stine, Prentice Hall; USB flash drive (highly recommended)
INCLEMENT WEATHER AND CLASS CANCELLATION POLICY
If severe weather is forecast, you should assume that TCC will be open unless you receive a TCC Alert. Signing
up for TCC Alerts is required; see TCC Homepage. TCC closings may also be announced on television and radio
stations. TCC is often not closed when the public schools are. However, your safety is of primary concern.
INSTITUTIONAL STATEMENT
Each student is responsible for being aware of the information contained in the TCC Catalog, TCC Student
Handbook, and Student Policies and Resources Handbook (on the TCC website), and semester information
listed in the Class Schedule and this Syllabus. NOTE: As of August 1, 2012, in compliance with Oklahoma
State law, Tulsa Community College must maintain a Tobacco Free Environment. Tulsa Community College
prohibits smoking or the use of tobacco products on all property owned, leased or contracted for use by Tulsa
Community College. This includes both indoor and outdoor areas such as, but not limited to, classrooms,
laboratories, offices, work areas, vestibules, hallways, enclosed walkways, restrooms, elevators, stairwells,
sidewalks, parking lots, and green spaces, etc.
EDUCATION ACCESS CENTER
It is the policy and practice of Tulsa Community College to create inclusive learning environments.
Accommodations for qualifying students in compliance with the Americans with Disabilities Act (ADA) and Section
504 of the Rehabilitation Act are available. To request accommodations, contact the Education Access Center
(EAC) at eac@tulsacc.edu or call (918) 595-7115. If a student is in need of accommodations, it is the student’s
responsibility to request accommodations and advise the instructor of the accommodations needed at least a
week before a test or assignment date for which accommodations are needed.
COMPUTER ASSISTED INSTRUCTION AND COMPUTER SERVICES ACCEPTABLE USE
Blackboard will be used for announcements, quizzes, tests, project directions, PowerPoints, notes, web links and
other instructional materials. You should check our Blackboard site and your TCC Outlook email at least three
times a week; preferably DAILY. It is highly recommended that you have regular (daily) computer access,
preferably a home computer with broadband Internet access. This course can be completed using public
computers at TCC or other public access areas. However, be aware that using public computers may create a
hardship. All course requirements remain the same whether your computer access is private (home) or
public. On the Southeast campus, computers are available in the Math Lab SEC 8150 (918-595-7751), the
Computer Lab SEC 4231, and the LRC. Access to computing resources is a privilege granted to all TCC faculty,
staff, and students. Use of TCC computing resources is limited to purposes related to the College’s mission of
education, research, and community service. See Student Policies and Resources Handbook. Students
consistently that take their tests at the same time, have the same pattern of incorrect answers or that have
frequent Blackboard “crashes” while taking their tests may be required to take their tests at a TCC approved
testing center.
Blended Microbiology Syllabus
page
2
LEARNING SUPPORT SERVICES
The SEC Math Lab, 8150, and Library (LRC) have copies of our textbook and Bergey’s Manuals. The SEC
Writing Center offers help in building analytical reading skills, solid research and effective writing skills and
provides information on writing styles (SEC 1102, call 918-595-7749 for appointment). Tutoring is free of charge.
ATTENDANCE, CELL PHONES AND OTHER ELECTRONIC DEVICES
Attendance for the online lecture portion of this class will be assessed by the completion of online assignments
and how often you access our website (at least three times a week.) You are required to attend every lab. If you
miss a lab, you must contact your instructor within one day and make arrangements to make up the lab prior to
your next lab. ATTENDING LAB REGULARLY, COMPELETEING BLACKBOARD QUIZZES, TESTS AND
ASSIGNEMENTS AND READING THE ASSIGNED TEXT AND LAB MATERIALS ON TIME are among the
most important factors in insuring your success in this class. For Lab, read all assigned materials and complete
your prelab questions and definitions BEFORE you come to lab. Lab absences or failure to complete your online
assignments, unknown identification, lab reports, and other assigned projects will lower your grade and may lead
to administrative withdrawal. Make sure you TURN OFF cellular phones and other electronic devices in lab. Text
messaging will lower your “attendance and participation” grade and will result in loss of credit for your lab exam.
Attendance and participation in lab and online performance is a component of your class grade.
WITHDRAWAL, AUDIT AND INCOMPLETE POLICY
It is the student's responsibility to drop or withdraw from courses by the scheduled deadline. Failure to withdraw
may result in the student receiving a grade of “F”. Non-attendance DOES NOT constitute official withdrawal. You
must have a passing grade, instructor approval and complete a contract to change from credit to audit or receive
an incomplete. Contact the Counseling Office at any TCC campus to initiate withdrawal from a course ('W' grade)
or to change from Credit to Audit. See the information about course withdrawal on Blackboard. The last day
to withdraw with a “W” or change from credit to audit is Friday, Nov. 6.
LABORATORY SAFETY
We will be working with live cultures in the laboratory. It is imperative that you follow all safety recommendations.
A lab coat is REQUIRED. You should wash your hands before and after each lab. Absolutely NO food or drink
is allowed in the laboratory. Please inform your instructor of any aspect of your health status (i.e. pregnancy,
medications, convulsions, etc.) which might impact your lab participation or performance.
ACADEMIC DISHONESTY OR MISCONDUCT
Academic dishonesty or misconduct is not condoned or tolerated at campuses within the Tulsa Community
College system. Academic dishonesty is behavior in which a deliberately fraudulent misrepresentation is
employed in an attempt to gain undeserved intellectual credit, either for oneself or for another. Academic
misconduct is behavior that results in intellectual advantage by violating specific standard, but without deliberate
intent or use of fraudulent means. The student should review the relevant sections of the Student Policies and
Resources Handbook.
ACADEMIC ACCOUNTABILITY AND PLAGIARISM POLICY
Students will have opportunities for learning in both group settings and as individuals in this class. While I
encourage you to study with your classmates, you are expected to complete your own labs, reports and research
projects. There will be zero tolerance for academic dishonesty or misconduct or deliberate plagiarism. No credit
plus an additional penalty will be given for work that is not your own or work of your own that you allow
to be copied. A student guilty of academic dishonesty or misconduct or deliberate plagiarism may
receive an “F” in the course. “Plagiarism is claiming, indicating, or implying that the ideas, sentences, or words
of another writer are your own; it includes having another writer do work claimed to be your own, copying the work
of another and presenting it as your own, or following the work of another as a guide to ideas and expression that
are then presented as your own.” Review the Plagiarism information on Blackboard.
Blended Microbiology Syllabus
page
3
EVALUATION TECHNIQUES
Online Lecture: For each chapter there will be PowerPoints, lecture notes, and often other content (animations,
web links,) as well as a practice Blackboard quiz covering the textbook reading assignment. The practice quizzes
for each chapter are worth 2 points. There are six Mastering Microbiology Assignments (for Exams 1-6), worth 5
or 10 points each. The Points Possible for each item is displayed in the gradebook. Completing the practice
quizzes and Mastering Microbiology Assignments will help you prepare for your test. About 30% -70% of the
questions on lecture exams will come from the practice quizzes and Mastering Microbiology assignments; other
questions on the test often cover similar content. The course is divided into 8 “Modules” (sections), each covering
from two to four Chapters. You will have a Module test every other week. Practice quizzes must be completed
prior to the test. Refer to the Schedule posted in Course Information the material covered in each test. You may
use your book, notes and other class resources on your lecture tests. Your tests are given on the honor system
and you must not either give or receive help on your tests. Students consistently that take their tests at the same
time, have the same pattern of incorrect answers or that have frequent Blackboard “crashes” while taking their
tests may be required to take their tests at a TCC approved testing center. A missed online exam may be made
up only if the instructor is notified prior the due date of the online exam. Make-up tests may have additional
written (essay, short answer, etc.) questions and must be completed within 1 week of the exam due date.
Students who miss an exam deadline may not be eligible to receive bonus or test curve points.
Class projects will include a Bergey’s Manual exercise and writing a Disease Report covering a pathogenic
microorganism. Information about these projects is posted in “Reports&Projects” on Blackboard. Late
assignments will not be accepted without instructor approval.
Content for this class will be posted both on Blackboard and Mastering Microbiology. If you have a used book or
purchased you book off campus, you must purchase access to Mastering Microbiology.
On campus Lab: Be prepared for lab. Lab definitions and research questions in italics and bold print are to
be completed BEFORE coming to lab. Lab reports will be checked at the beginning of lab for pre-lab
preparedness and labs reports may be due in lab at the end of the period the lab is completed. Lab quizzes may
be given in lab prior to or following the lab. Contact your instructor immediately if you miss lab. Due to the special
materials needed, missed laboratory classes may be made up only with the instructor’s approval. Excessive
absences from lecture, lab and/or failure to complete your Unknown may result in Administrative Withdrawal from
the course. No electronic devices, cell phones, ball caps, dark glasses or notes (unless specifically stated) may
be used during lab tests.
If you have questions about any grade received in this class, such as your grade for a test, assignment, lab, or
report; you must speak with and send a TCC Outlook email to your instructor within one week after receiving that
grade and work with your instructor to resolve the issue as soon as possible.
EVALUATIONS
Possible Points
Grading Scale
Practice Quizzes
50 points
A: 900-1000 points
Mastering Microbiology Quizzes
55 points
B: 800-899 points
Lecture Exams
400 points
C: 700-799 points
Bergey’s Project
25 points
D: 600-699 points
Disease Report Research Paper
75 points
F: below 600 points
Laboratory Reports
200 points
Lab Exam
100 points
Unknown
50 points
Attendance and Participation
45 points
Total Points:
1000 points
Extra Credit: Disease PowerPoint, additional Unknown documentation, seminars (see Blackboard)
THIS IS NOT A SELF-PACED COURSE!
You must complete assignments, reports and tests during the time they are scheduled!
Blended Microbiology Syllabus
page
4
RESEARCH ASSIGNMENTS
Bergey’s Project. 25 Points. This short project is designed to help you become more familiar with how to use
Bergey’s manuals, which you will use to help identify your Unknown. A form to use for your Bergey’s project and
a sign-up sheet will be available in lab.
Written Disease Report. 75 Points.
Select a bacterial, protozoan, fungal, viral or prion disease. Look in Chapters 19-25 in our text, in Topley &
Wilson’s Microbiology and Microbial Infections (one of the excellent resources available on reserve in the
reference section in our LRC) or in the Bacterial Pathogens, Mycology, and Parasitic Protozoans Exercises in our
lab manual if you need some suggestions for your topic. Each student must choose a different disease for his or
her report. A sign-up sheet will be available in lecture. The same bacteria may, but does not have to, be used for
your Bergey's project and your disease report. If you choose to do your disease report on a disease not
associated with the bacteria used for your Bergey's project, you may “release it” so that another student may use
that topic for their report.
Review the information on Plagiarism on Blackboard and Handouts before you begin writing. Write a paper on
your topic. Include the classification of your organism in standard format (“Bergey Project” format for bacteria but
no Roman numerals required), in your paper. The text of your paper EXCLUDING title page information (title,
your name, class, date), section headings, spaces between paragraphs, illustrations, charts and any other
materials that you did not write should be at least five pages double spaced, with 1 inch margins, 12 point font
(Times New Roman, Arial or similar font.) You are allowed 3 lines for your classification information. Your paper
should be in the format of review article and may have a 1/4 - 1/3 page abstract. You should have five (or more)
references, cited in the format used for scientific journals (such as Nature), for your bibliography.
Your references should include:
1. A full text peer reviewed article, with references, from a refereed journal. For ONE peer-reviewed article, turn
in a copy of ONLY the title (first) page of the peer-reviewed article and one page of the bibliography
(usually last or next to last page of the paper).
2. One scientific journal article that is at least 2 pages long.
3. A recent journal article (~1 year). NOTE: One article may, but does not have to, fulfill requirements 1. - 3.
4. An article from the CDC website (see link on Blackboard in Projects&Reports). See me for an acceptable
alternative if needed.
5. Other references may include Topley & Wilson’s Microbiology and Microbial Infections (available in our LRC)
your text (if applicable) and Internet websites.
6. NOTE: You may NOT use Wikipedia as one of you five required references. If you do use Wikipedia, you
must correct the classification scheme to be consistent with Bergey’s Manual.
You must submit your report to SafeAssignment on Blackboard and receive a plagiarism score of 25% or less.
Directions for submitting your paper to SafeAssignment will be posted on Blackboard. You should post your
disease report file on the “Disease Report Parking Spot” in Reports&Projects on Blackboard for safekeeping.
Turn in a printed copy of your paper, with references, in lecture on the due date. Keep a copy of your paper to
use for your presentation (and as a back-up in case the Lost Assignments monster eats it.) The paper you turn in
will not be returned.
You will have the opportunity to make a ~ 10 minute presentation of your disease report for 10 points extra credit
when we cover diseases in lecture. Following the discussion of your disease presentation, you will present four
objective questions to the class and post your questions on our TCC Outlook "My Consolidated Courses" site.
Exam questions will be selected from student presentation questions.
Some Recommended Journals (there are many more excellent journals)
Journal of Cell and Molecular Biology
Journal of Microbiology Science News
Discover
Emerging Infectious Diseases
J. of Infectious Disease Clinical Infections Nature
New England Journal of Medicine
Journal of Bacteriology Scientific American Lancet
Virology
Cell
Science
Many of these journals are in the TCC LRCs. Feel free to use journals from other libraries. Many journal articles
are available online. If you use Internet resources, also include the web address in your bibliography.
Blended Microbiology Syllabus
page
5
MICROBIOLOGY BIO 2164 310 TENTATIVE CLASS SCHEDULE 1-11-13 INSTRUCTOR: MARVA VOLK
NOTE: The class schedule may be changed AT ANY TIME by updates posted on Blackboard
or verbal notification in class
Week LECTURE TOPICS (Lecture materials to be covered
LABORATORY (Friday)
BEFORE Lab on Friday)
Date
Complete Prelab BEFORE Lab
All Blackboard and Mastering Quizzes must be
(Friday)
Lab coat & Safety Glasses Required
submitted before the Lecture Exam.
(for Labs 2-9)
1
Module 1: Introduction to Microbiology
Blackboard and LRC Orientation. Meet in
Chapter 1: A Brief History of Microbiology
Micro Lab 8204 then Computer Rm 8152
Chapter 2: The Chemistry of Microbiology
18-Jan
2
Chapter 3: Cell Structure and Function
Lab 1 Microscope & Bacterial Morphology
(No Classes MLK Day, Mon. Jan. 21)
Chapter 4: Microscopy, Staining and Classification
Exam 1 Ch 1-4
25-Jan
3
Module 2: Metabolism and Growth
Lab 2 Aseptic Technique & Isolation Streak
Chapter 5: Metabolism (omit photosynthesis,
biosynthesis p. 149-158)
1-Feb
4
Chapter 6: Microbial Nutrition and Growth
Lab 3 Gram Stain
Exam 2 Ch 5&6
8-Feb
5
Module 3: Genetics
Lab 4 Spore Stain & Acid Fast Stain
Chapter 7: Microbial Genetics
15-Feb
6
Chapter 8: Recombinant DNA
Begin Unknown Journal: Part I. Streak, Ana Jar
Sign-up for Bergey Project
Lab 5 Negative Stains: Nigrosin & Capsule
Exam 3 Ch7&8
22-Feb
7
Module 4: Microbial Control &Prokaryotes
Begin Lab 6 Selective & Differential Media
Chapter 9: Control of Microbial Growth (External)
Unknown Journal: Part II. Unknown Gram
Chapter 10: Antimicrobial Drugs (Internal)
Stain, Catalase & Oxidase tests, (Morphology)
1-Mar
8
Chapter 11: Prokaryotes
Complete Lab 6 Selective & Differential Media
Bergey's Project Due Sign-up for Disease Report
Unknown Morphology
Exam 4 Ch 9-11
8-Mar Notebook Check: Turn in Labs 1-6
9
Module 5: Eukaryotes, Viruses & Epidemiology
Lab 7 Inoculate Biochemical Tests; Biochem
Charts Due at BEGINNING of Lab
Chapter 12: Eukaryotes
Chapter 13:Viruses
15-Mar
10 Chapter 14: Infection & Epidemiology
Spring Break No Lab
Exam 5 Ch 12-14
22-Mar
11 Module 6: Infection and Immunity
Lab 7 Read & Turn In Biochemical Tests
Gloves are REQUIRED for this lab!
Chapter 15: Non-Specific Immunity
Chapter 16: Specific Immunity
Inoculate broth for Lab 8 (begin working on Lab
Disease SafeAssign Due by 10 pm Wed. March 27
29-Mar 8; prelab due next week)
12 Chapter 17: Immunization
Lab 8 prelab due. Begin Lab 8 Effect of UV
Chapter 18: Immunological Disorders
Radiation&Antibiotic Sensitivity Testing
Exam 6 Ch 15- 18
Work on Unknown Identification in Lab
Disease Research Paper Due in lab Fri. April 5
5-Apr Begin working on your Lab 9 Charts!!!
13 Module 7: Bacterial Diseases
Finish & turn in Lab 8 UV & Antibiotics
Begin Lab 9 Eukaryotic Microorganisms: Fungi
Diseases: Chapter 19 & 20
Last Day to withdraw/audit: Fri. April 12
12-Apr
14 Diseases: Chapter 21 & 22
Complete Lab 9 Eukaryotes: Protzoans
Exam 7 Ch 19-22
Turn in Lab 9 AND Lab 9 Chart
19-Apr Turn in Unknown Identification
15 Module 8: Protists and Viral Diseases
Lab Exam Review
Chapter 23, 24 & 25
26-Apr
16
Exam 8 Ch 23-25
Lab Exam
3-May
No Lab
10-May
Blended Microbiology Syllabus
page
6