Photosynthesis Copyright © 1999 Rollo Silver What is photosynthesis? The ability to convert light energy into chemical energy in the form of sugar. PHOTO = light SYNTHESIS = to make, manufacture Why is photosynthesis so important to me? #1: One product of photosynthesis is glucose (sugar) that is the basis of most food chain. #2: The second product of photosynthesis is oxygen. Since you are an aerobic organism, you require oxygen in order to live. www.sclero.org Where does photosynthesis occur? Autotrophs: self feeders because they make their own food. Ex: plants Plants: in leaves that contain organelles called chloroplasts Where does photosynthesis not occur? Heterotrophs: organisms that cannot use the sun’s energy to make its food. Have to eat other things in order to make energy. Ex: humans and animals What raw materials are needed for photosynthesis? Sunlight: That’s why it is called PHOTOsynthesis Water Carbon dioxide (CO2) Chlorophyll www.hhcamp.com How can we summarize photosynthesis? CO2 + H2O ------Sunlight------ C6H12O6 + 6O2 Carbon dioxide Glucose (sugar) oxygen Water What else do I need to remember about photosynthesis? It is important in the oxygen-carbon dioxide cycle. Carbon dioxide is being removed from the air while putting oxygen back into the air Increase in carbon dioxide levels contribute to Global Warming. Where does the glucose (sugar) go after the leaves make it? The sugar is stored in various forms in different parts of the plant Stems: celery, rhubarb Roots: carrots, turnips, radish Fruits: apple, banana Seeds: rice, wheat, barley, corn Leaves: spinach , lettuce www.thefeltsource.com How are plants adapted for photosynthesis? The leaves of plants are the factories where photosynthesis occurs Let’s take a look at some structures in the leaves Photosynthesis occurs in organelles called chloroplasts Inside the chloroplasts, photosynthesis occurs in the grana and stroma WHY ARE PLANTS GREEN? Inside the grana are huge amounts of a pigment called chlorophyll. Chlorophyll absorbs blue, violet, orange and red light and reflects green and yellow light www.yorku.ca Photosynthesis occurs in two phases Light –Dependent Reactions Light – Independent Reactions or the Calvin Cycle Light-dependent reactions Reaction requires sunlight First phase of photosynthesis Occurs in the grana Helps form needed materials for the second phase of photosynthesis called light-independent reaction or Calvin Cycle •NADPH2 •ATP BUT how does this all happen? Through a series of complex reactions that looks long on paper but the reaction occurs in a fraction of a second. We are going to describe the light-dependent reaction in a series of steps. Light-dependent reaction STEP 1 Chlorophyll is energized Chlorophyll (the pigment) in chloroplasts absorbs sunlight and becomes energized or excited. Chlorophyll passes the energy it captured from the sun through the electron transport chain and produces energy for reactions. Light-dependent reaction Step 2 Formation of ATP Chloroplasts contain ADP. Some of the energy released during the electron transport chain helps add another phosphate to ADP to form ATP. A + P + P ------ + P yields A + P + P + P ATP stores energy that will be used in the second phase called the Calvin Cycle Light-dependent reaction Step 3 Splitting of water molecules Some of the other energy produced during the electron transport chain goes to split water into hydrogen and oxygen molecules. Oxygen is released back into the air The two hydrogen ions are trapped by a coenzyme in the chloroplasts called NADP and forms NADPH2 which will be used on the Calvin Cycle Light Independent Reactions or Calvin Cycle Second phase of photosynthesis Does not require sunlight Uses carbon dioxide (CO2) to form glucose This reaction occurs in the stroma of the chloroplasts Light Independent Reaction/ Calvin Cycle Uses the products made in the light dependent reaction (ATP and NADPH2) These provide the energy to do the following steps. Combines H+ with CO2 “carbon fixation” H+ came from the light reaction CO2 came from the air which entered the leaves thru the stomata Remember the purpose of this whole mess is to make glucose. C6H12O6 To build a glucose molecule we need C, H, and O which we have in our H+ and CO2 A whole series of complicated chemical reactions causes the H+ and CO2 to form a 3-carbon compound called PGAL Each PGAL is like a ½ of a glucose molecule. So through another series of reactions two PGAL combines and presto! A glucose is made.