Lab. Safety, Microscopes
and
Colony Morphology
Supplemental instruction
Fall 2011
Chattahoochee Technical College
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Lab. Safety
Food and Drink Not allowed.
Safety goggles, Please….
Dispose materials in proper place.
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Dispose materials
Bacteria containing solid materials.
To biohazard can.
Microscopic slides
To glass disposal box
Bacteria containing liquid test tubes.
To waste rack
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Dispose agar plates
In order to dispose agar plate, wrap the
plate with lab. film before putting the
biohazard can.
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What type of microscope are
you seeing?
Light compound microscope
In which object do you need immersion oil to see the bacterial
sample? 100X objective
Why do you need oil-immersion? (how the oil-immersion work?)
Bacteria is too small; Oil – to increase the magnification and to
remove light refraction and to improve resolution.
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What are the names of these parts? Write each function.
A
The answer is in your textbook or lab. notebook.
B
E
C
D
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40
X
10
X
100X
Total magnification on the slide in view?
100x
10
X
Total magnification on the slide in view?
400x
100X
40
X
You want to see in 600 times magnification. Which part do
you change to? (turn into..)
60x objective
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Colony Morphology
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Colony Morphology and Arrangements
-Cocci
•Diplococcus: cocci arranged in pairs.
•Streptococcus: cocci arranged in chains
•Staphylococcus: cocci arranged in
irregular, often grape-like clusters
Diplococcus
Staphylococcus
Streptococcus
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Colony Morphology and Arrangements
-Bacillus(Rods)
• Bacillus: a single bacillus
• Diplobacillus: Bacilli that remain in pairs
after they divide.
• Streptobacillus: bacilli in chains
• Coccobacillus: oval and similar to a
coccus
Bacilli
Streptobacilli
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