Photosynthesis Defined: process by which light energy is converted to chemical energy. • Is this an example of metabolism? • Is this a chemical reaction? Autotrophs: self feeding Energy: ATP Light- Absorbing Structures • Pigments: molecule that contains a lightabsorbing structure • Chlorophyll: primary pigment involved with photosynthesis • Carotenoids: secondary pigments in photosynthesis Color- absorption vs. reflection • What visible light colors (wavelength) does chlorophyll A and B absorb? Reflect? Infrared (IR) light • Invisible to human eye • “Night Vision” • “Thermographic” cameras • Photosynthetic Bacteria Prokaryotes – Lack organelles – Single celled Cyanobacteria Who came first? Endosymbiosis Recap Story of Life • Atoms Molecules • Molecules Organic compounds: monomers • Monomers Polymers – Amino acids protein and enzymes – Glycerol & fatty acids lipids • Phospholipids selective permeability • Maintain Homeostasis • Photosynthesis- Carry out Metabolic Reactions Photosynthesis Stages 6CO2 + 6H2O + light energy C6H12O6 + 6O2 Stages of Photosynthesis: i. Absorb light energy (via pigments) ii. Convert light energy chemical energy iii. Convert chemical energy organic compounds Photosynthesis Light Reaction Steps Calvin Cycle (Light- Independent) Occurs in the Thylakoid Membrane Chloroplast Occurs in the stroma chloroplast 1. 2. 1. 3. 4. 5. 6. 7. Light energy is absorbed Water molecules are split into H+ ions, O- and e-. O- combine to form O2 that diffuses out of thylakoid membrane H+ ions travel down concentration gradient through the transport protein ATP synthase via facilitated diffusion. ATP synthase uses energy from H+ gradient to combine ADP + Pi ATP (chemical energy) Light energy excites electrons, which bounce through the Electron Transport Chain (ETC) and land on NADP+ reductase, enzyme. NADP+ reductase combines e- + NADP+ NADPH Inputs: H2O + light energy Intermediates: H+ ions, Oxygen and Electrons Outputs: ATP and NADPH 2. 3. 4. CO2 is added to a 5-C compound via enzyme RuBisCO to make a 6-C compound. The resulting 6-C compound splits into two 3C compounds; phosphates from ATP and electrons from NADPH are added. One of the 3-C compounds is used to make C6H12O6, a sugar to store energy for plant. The other 3-C compound gets recycled back into the mix to keep cycle going. Inputs: ATP, NADPH and CO2 Intermediates: 3- carbon sugar Outputs: C6H12O6 6. 7. 6. 2. 3. 4. 5. + 5 carbon compound RuBi sCO Unstable 6 carbon compound 3 carbon simple sugar (recycled) organic compounds: sucrose, starch