Biology Unit Exam Review Microscope Total Magnification= eyepiece X objective 1. 10 X 40= 400 2. High Power Key Terms for Microscope 3. Magnification: see first slide for total magnification 4. Resolution: show two points that are close together as separate images 5. Slide moves to left, image appears to move to the right Parts of the Microscope 1. 2. 3. 4. 5. 6. 7. 8. Ocular/Eyepiece Fine Adjustment Knob Objective Lenses Diaphragm Base Light Source Stage Arm Fine vs Coarse Adjustment 7. Course Adjustment Knobmoves lenses 8. Course- low power Fine- High Power MAKE SURE THAT YOU KNOW THE DIAGRAM OF THE MICROSCOPE AND THE CHARACTERISTICS OF LIFE Scientific Method Steps of Sci Method: Problem Hypothesis Testing Hypothesis (procedure) Record Data (# or details) Analyze and Form Conclusions Replicate Work Variables Controlled Experiment: Two groups differ by one factor; control is present Control vs Experimental Group: Experimental group has something changed (i.e. amount of light, etc) Variables Independent Variables: Changed by scientist to see what happens Dependent Variable: Changes in response to the change the scientist makes to the independent variable. Determine the Different Variables Does fertilizer make a plant grow? Two spider plants are placed in separate pots. One plant is given fertilizer and the other plant is not. Both plants are measured daily. Identify Independent, Dependent, and control group Answers INDEPENDENT: Amount of fertilizer DEPENDENT: Growth of plant CONTROLLED: Plant not receiving fertilizer Chemistry Atomic Number, Atomic Mass Atomic #: # of protons in the nucleus Atomic Mass: sum of protons and neutrons in the nucleus Example: Oxygen Atomic #: 8 Atomic Mass: 16 Determine the # of protons, neutrons, and electrons ANSWER Protons: 8 Electrons: 8 Neutrons: 16-8= 8 Charges Protons: + Electons: Neutrons: neutral Neutral Atom Neutral atom has no charge; so number of protons and electrons would be equal Ions Charged Charge atom can be either positive or negative…how does this form? ANSWER Positive Ion: excess positive charge ( more protons) Negative Ion: excess negative charge (more electrons) Positive or Negative Ion? Isotope of potassium has 19 protons and 21 electrons. What is its charge? ANSWER NEGATIVE Covalent Bonds Bond formed by sharing electrons Ionic Bond Bond that forms by transferring the electrons; can create an electrical attraction Elements, Compound, Mixture Elements: substance made up of one kind of atom (i.e. oxygen) Compound: made up of two or more elements (i.e. water) Mixture: substances that are mixed without forming a new chemical bond (i.e. saltwater) 2H2O + O2---2H2O2 Atoms= hydrogen, oxygen Reactants: 2H2O + O2 Products: 2H2O2 Atoms: 8 Hydrogen, 8 Oxygen Chemical Formula Tells you the type and amount of atoms EX: H2SO4 2 hydrogen, 1 sulfur, and 4 oxygen H2SO4 Atoms: Hydrogen, Sulfur, Oxygen H= 2 S=1 O=4 pH Scale pH runs from 1-14 7 is neutral Below 7 acidic (with 1 being more acidic) Above 7 basic with 14 being most basic Neutralization Reaction Reaction that occurs when acids and bases are combined to form water and salt EX: NaOH + HCl----NaCl + H2O Solution & Its Parts Substances are spread evenly throughout the mixture EX: Air- comprised of N, O, CO2 Two parts: a. b. Solvent: substance that a solute is dissolved in Solutes: substance that is dissolved in a solvent Biochemistry Organic Compounds Compounds that contain carbon and occur naturally only in the bodies and products of an organism Monomer vs. Polymer Monomer- Polymer- 1 sugar 2+ sugars; can contain 100 or 1000 sugars Macromolecule Another name given to organic compound because they are very large compounds Monomers Carbs: sugar Proteins: amino acids Nucleic Acid: nucleotide Skip question #6 Examples of Carbohydrates Anything that ends with –ose (maltose, fructose, glucose) Examples: Lipids Proteins Fats, oils, waxes Enzymes Nucleic Acids DNA, RNA Classify: A. carb B. carb C. Lipids D. Lipids E. Lipids F. Nucleic Acids G. Nucleic Acids H. Proteins I-K. Carb Disaccaharide: 2 sugars (maltose) Polypeptide: 2+ amino acids connected by peptide bonds Polar vs. Nonpolar Water is polar Polar molecule: a molecule that has a partial positive and partial negative charge Only polar substances will dissolve in water; non polar will not. Enzymes 12/13. Proteins that are necessary for life 14. It is a catalyst, which causes a reaction to occur 15.pH, temperature affect enzymes 16. Substance that is broken down by enzyme 17. Area where substrate binds The Cell: Make sure you know the diagrams on plant and animal cells Cell Theory 1. All living things are composed of one or more cells 2. Cells are organisms’ basic units of structure and function 3. Cells come only from existing cells Prokaryotic vs. Eukaryotic Cells Prokaryotes: Lack any internal membrane bound structures (most organelles) and nucleus Ex: bacteria Eukaryotes: have nucleus; plants & animals Cell Membrane Also called Plasma Membrane Prokaryotes, Eukaryotes Plants & Animals Function: controls what enter and leaves the cell Cell Wall Found in bacteria and plants Made of cellulose Function: gives cells its shape and protection Nucleus & Nuclear Envelope Only in eukaryotes Function: Control center for metabolism and reproduction Surrounded by a nuclear envelope that is selectively permeable Nucleolus Eukaryotes Function: makes ribosomes Cytoplasm Eukaryotes & Prokaryotes Jelly-like substance in between cell membrane and nucleus Function: metabolism; holds organelles in place Endoplasmic Reticulum Eukaryotes Function: highway of cell; moves material from one end to the next Two Types: Rough Endoplasmic Reticulum (ER): ribosomes attached Smooth ER: no ribosomes Ribosomes Eukaryotes Function: makes proteins Golgi Complex Eukaryotes Flattened stack of pancakes Modify and transport proteins to parts of the cell Lysosomes Eukaryotes Garbage disposal; break down food or bacteria Mitochondria Eukaryotes Powerhouse of cell Produces energy for the cell Vacuoles Plants: one large Animals: several small that digest food or remove excess water (similar to sponge) Cilia & Flagella Involved in cell movement Cilia: usually in animals Flagella: usually one whip like structure found in unicellar organisms Microtubules & Microfilaments Involved in cell movement and shape Forms the cell’s cytoskeleton Eukaryotes Centrioles Involved in cell division Animals only Plastids Plants Two only types: 1. Leucoplasts: starch storage 2. Chromoplasts: pigments for color Choloroplast Type of chromoplast Contains chlorophyll; site for photosynthesis Plants only Phospholipid Bilayer Selectively Permeable Only allows certain things to enter or exit EX: Cell Membrane Organization Cell Tissue Organ (stomach, pancreas, liver) Organ System (respiratory, circulatory) Organism (human, dog, cat) Tissue vs. Organ Tissue: group of cells that serve the same function Organ: group of tissues that serve the same function Cell Transport Active vs. Passive Active Energy Required Moves from low concentration to high concentration Passive No energy required Moves from high concentration to low concentration Diffusion Movement of molecules from an area of high concentration to an area of lower concentration Passive Transport Diffusion Concentration Gradient Occurs when there is a difference between high and low concentration Equilibrium Same amount is inside and outside the cell Osmosis Diffusion of water across a selectively permeable membrane from region of high water concentration to a region of low water concentration Passive Plasmolysis vs. Cytolysis Plasmolysis: shrinking of cytoplasm (result of hypertonic solution) Cytolysis: cell explodes due to hypotonic solution Turgor Pressue Increase of pressure in a plant due to water entering the cell via osmosis Plant cell will not explode because of cell wall Greatest pressure in pure water Facilitated Diffusion Special transport proteins transport substance into or out of cell Same as diffusion- movement from high to low concentration Endocytosis Transporting material into cell by vesicle EX: Pinocytosis: small liquids are taken into cell by vesicle Phagocytosis: solid particles ingested into cell by vesicles Exocytosis Transporting material out of cell by vesicle Type of Solution Where is there more solute Where is there more solvent (water) What direction does water move What happens to the cell Hypotonic Inside cell Outside cell Inside Cytolysis (explode) Hypertonic Outside cell Inside cell Outside Plasmolysis (shrink) Isotonic Equal Equal Constant Nothin Let’s Do Examples Look at the examples on the board and determine which way the water will move and what will happen to the cell.