What invention helped with many
discoveries in science?
Agenda for Friday Sept 18th
1. Microscope notes
2. Microscope lab
What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by
the power of the objective.
Eyepiece is 10x and
objective is 10x what
is total magnificaion?
Basic Rules and Tips
•
•
•
•
•
Carry using arm and base
Flat Surface
Adjust light using diaphragm
Use stage clips
Focus with course adjustment on low and
medium power only!
– High Power use fine adjustment only
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the
water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover
Slip
Lower slowly
Comparing Powers of Magnification
We can see better details with higher the powers of
magnification, but we cannot see as much of the
image.
Which of these images would
be viewed at a higher power
of magnification?
How do you calculate total
magnification?
Agenda for Monday Sept 21st
1. Measure with microscope notes
2. Labs
Worm lab due tomorrow
Quiz Tuesday
Test Thursday
Calculate Field of View
• Clear Ruler
• Determine how many millimeters across
• Convert to micrometers
• 1mm=1000μm
• So: if 1.5 mm across then field of view is
1.5mm x 1000 μm
= 1500 μm
1mm
one millimeter is the distance
from the middle of one mark to
the middle of the next mark.
Calculate Size of Specimen
• Figure out how many will fit across the field of
view (estimate)
• Divide field of view length by how many fit
across
Example:
*About 8 fit across
– field of view is 800 μm
– 800 μm/8 = 100 μm
Remember!
•
•
•
•
•
Carry using arm and base
Flat Surface
Adjust light using diaphragm
Use stage clips
ONLY USE FINE ADJUSTMENT ON HIGH POWER!
How many microns(micrometers) is
3.2 mm?
Agenda for Tuesday Sept 22nd
1. Quiz
2. What is life notes
3. Finish labs
TEST Thursday
• Low power = 4 mm or 4000 μm
• Medium power = 1.5 mm or 1500 μm
• High power = .5 mm or 500 μm
Lab Questions
1. a. If I measure the low power field of view to be
7 mm, how many μm is that?
b. If I estimate that 9 of my specimen could fit
across the field of view, how big is each one in
mm, and μm?
c. How big would the high power field of view
be if the low power objective is 4x and the high
power is 50x?
2 a. If the high power field of view is 425 μm, how
many mm is that?
b. If I estimated that 2.5 specimens could fit
across the field of view, how big is my specimen,
in mm and μm?
c. If each specimen is 30 μm, and 10 can fit
across, how big is the field of view?
d. If each specimen is 120 μm and the field of
view is 630 mm, how many can fit across?