Name:_____________________________
Date:______________________________
Semester:____________
Banker
Lab 25: Brain Dissection
Practical 4
Reading: No new reading from the book
Figures: No figures from the book. See the figures from my PowerPoint.
Locate on Diagram/Model:
Convolution
Diencephalon
Fornix
Lateral Sulcus
Transverse fissure
Olfactory Tract
Mammillary Body
Pineal Glad
Lateral Ventricles (First and Second)
Sulcus
Brainstem (Midbrain, Pons, Medulla oblongata)
Central sulcus
Temporal lobe
Cerebellar hemisphere
Optic chiasma
Optic Tract
Corpora Quadrigemina
Third Ventricle
Cerebrum (Cerebral Hemesphere)
Corpus Callosum
Frontal Lobe
Parietal Lobe
Olfactory bulb
Optic nerve
Pituitary Gland
Thalamus
Fourth Ventricle
Locate on Dissection:
Convolution
Frontal Lobe
Transverse fissure
Cerebrum
Thalamus
ArborVitae
Medulla Oblongata
Mammillary Body
Olfactory Bulb
Sulcus
Temporal lobe
Cerebellum (Cerebellar hemisphere)
Corpus Callosum
Midbrain
Spinal Cord
Pons
Optic Chiasma
Olfactory Tract
Cerebrum (Cerebral Hemesphere)
Parietal Lobe
Pineal Gland
Lateral Ventricles
Cerebellum
Central Canal
Pituitary Gland
Optic Tract
Corpora quadrigemina
Lab:
1.
2.
Obtain a preserved sheep brain, and rinse with water.
Examine the outer surface to see the meningies. You should at least be able to see the tough outer Dura Mater and the inner Pia Mater
(adheres to the surface of the brain). The Arachnoid Mater may be hard to see.
3. Remove the Dura matter carefully. Do this by carefully pulling it from the brain. In some places you may need to use scissors or a scalpel.
4. Examine the dorsal surface of the brain facing upward, and locate the features listed above.
5. Gently Separate the cerebral hemispheres, and expose the transverse band of white matter (corpus callosum).
6. Bend the cerebellum and medulla down slightly (do this gently). This should expose the pineal gland and Corpora quadrigemina.
7. Examine the ventral surface of the brain facing upward, and locate the features listed above. (some of the cranial nerves may be missing, but
you should be able to see the structures relating to the optic and olfactory nerves)
8. Using a scalpel cut the sheep brain along the midline (between the cerebral hemispheres). This will produce a midsagittal section. Examine
the midsagittal section of the brain facing upward, and locate the features listed above.
9. When finished, bring the brain to the teacher. I will either instruct you to dispose of the brain or keep it (if the dissection id particularly good).
10. Wash all tools and equipment. Replace them in their proper places.
Extras:
1.
2.
Remember to use gloves!
Do not wear nice clothes for this lab. You will likely get some preserved tissue or preserving fluid on your clothes of shoes.