1 ENVIRONMENTAL AND POLLUTION MICROBIOLOGY PROBLEM SET DUE APRIL 28, 2010 1. Write a balanced equation for the mineralization of 2,4-dichlorophenol to carbon dioxide under sulfidogenic conditions. You need not consider the production of cells. 2. Write a balanced equation for the degradation of toluene to carbon dioxide under iron reducing conditions (Fe+3 —> Fe+2). You need not consider the production of cells. 3. Compare the aerobic oxidation of glucose to carbon dioxide and water (ignore cell production) with the aerobic oxidation of a comparable 6 carbon alkane (ignore cell production). What is the difference in oxygen required? If you were asked to stimulate the biodegradation of a 50,000 gallon spill of this alkane (~ 40 tons of petroleum) in a coastal environment, how much nitrogen (in tons) would be needed (assume a 10:1 ratio of C:N)? What kinds of ecological consequences could result by adding this nutrient to this coastal environment site?