課程大綱及進度表
開課系所
生命科學系
開課學年
98
開課學期
1
課程名稱(中文)
微生物學實驗
課程名稱（英文）
Microbiology Laboratory
課程碼
C531800
分班碼
先修科目或先備能力
學分數
1
開課教師
王浩文
e-mail
hvwang@mail.ncku.edu.tw
電話
65538
Office Hours
課程概述
An introduction to experimental
techniques in microbiology.
The exercises include cultivation and
various staining techniques, isolation,
identification and biochemical activities.
Three hours of laboratory per week. A
brief lecture will be given prior to each
experiment. A team of two students are
expected to construct or set up their own
experiment. Materials are usually
prepared by a laboratory assistant.
Students are expected to develop
marginal laboratory techniques. The
result of each experiment should be
recorded and discussed.
教學目標
You are expected to become
familiar with (1) observation and
manipulation of microorganisms,
(2) preparation of media and
culturing, (3) the utilization of
stains to observe microbial
structures and other tests for
microbial identification, and (4)
develop independence on research
skills and sources of scientific
information.
授課課程大綱明細
1.Introduction
Culture Transfer Techniques
Techniques for Isolation of Pure
Cultures
2. Cultural Characteristics of
Microorganisms
Maintenance and Preservation of
Pure Cultures
3. Microscopic Examination of Living
Microorganisms
The Microscopic Measurement of
Microorganisms
Preparation of Bacterial smears
Negative Stain
4. Bacterial Stain
Simple Stain
Gram Stain
Acid-Fast Stain
Spore Stain
5. Nutritional Requirements : Media
for the Routine cultivation of
Bacteria
Use of Differential and Selective
Media
6. Physical Factors: Atmospheric
Oxygen Requirements
Techniques for the Cultivation of
Anaerobic Microorganisms
7. Serial Dilution-Agar Plate
Procedure to Viable Cell
The Bacterial Growth Curve
8. Biochemical Activity of
Microorganisms
Extracellular enzyme
Triple sugar iron agar test
Utilization of amino acid
9. IMViC
H2S production
Urease test
10. Carbohydrate fermentation
Carbohydrate fermentation
Catalase test
Oxidase test
11. Microbiology of Water
12. Chemical Agents of Control:
參考書目
Chemotherapeutic Agents
The Kirby-Bauer Antimicrobial
Sensitivity Test
13. Phenol Coefficient
14. Enzyme-Linked Immunoabsorbent
Assay
1. Cappuccino & Sherman,
Microbiology: A Laboratory
Manual,8th ed. 2007
Benjamin/Cummings Publishing
Company
2. Bergey's manual of systematic
bacteriology / David R. Boone, Richard
W. Castenholz, editors, volume 1 ;
George M. Garrity, editor-in-chief. 2nd
ed. New York :Springer,2001-
課程要求
評量方式
實驗報告: 50%
隨堂考: 15%
期末考: 20%
平時及值日生工作態度: 15%
課程網址
助教資訊
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