BY 240, Applied Microbiology (Summer Quarter, 1995)

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Instructor:

Meeting Time:

BY 340-01,-02 Microbiology Course Syllabus, Fall Semester, 2002

Dr. Donald Salter, BG 201C (office) or BG 206 (laboratory) or BG 108

(mailbox); Phone No .

205-652-3416 (voice mail); email: dsalter@uwa.edu. http://facstaff.uwa.edu/dsalter. Office Hours: MF, 2:30 - 5:30 PM; Tu, 1:30 -

5:30; other times by appointment (by phone (voice mail) or email).

Lecture: 1:25 – 2:15 PM MWF, Room 109 BG. No lecture on M, September

2 (Labor Day), MWF, November 25, 27, & 29 (Thanksgiving Holidays).

Laboratory: Section 01: 8:00 - 10:45 AM TH; Section 02: 12:30 -3:15 PM

TH; Room 206. No lab on W, November 27 (Thanksgiving Holidays).

Course Description: "Concepts and techniques relating to the morphology, taxonomy, physiology, and cultural methods of microorganisms, with emphasis on those of special importance to humans."

Credit Hours: 4 semester hours.

Prerequisites and BY 112 (General Botany) OR BY 122 (General Zoology). In addition, the

Course Standards: student must have the ability to read, perform simple math and algebra, and write effectively.

Course Objectives: 1. To become familiar with the biology, chemistry, genetics, growth, and function of the microbial community.

2. To become proficient in using various techniques to study the structure, function, and identification of microbes.

Course Materials: Lecture Text: “Foundations in Microbiology: Basic Principles”, softcover,

K.P. Talaro and A. Talaro, 4th Edition, 2002, WCB McGraw-Hill, Boston,

MA, ISBN 0-07-232042-7. 2 nd

and 3 rd

editions would probably be ok too.

Laboratory Manual: “Applications in General Microbiology: A Laboratory

Manual” T.J. Kerr and B.B. McHale, 6th Edition, 2001, Hunter Textbooks,

Inc., Winston-Salem, N.C., ISBN 0-88725-269-9.

Lab Material: A lab coat or lab apron, safety glasses, and disposable gloves are recommended for you to wear at appropriate times in the laboratory. I have enough of each that can be shared by the students. If you have a lab coat or safety glasses from work or left from another class, please mark them appropriately and bring them to leave at class. Please purchase some sort of marking device (Sharpie, Grease Pen, etc.) for marking test tubes, Petri dishes.

Computer: You can access the course materials through my WEB page:

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http://facstaff.uwa.edu/dsalter .

You should be able access various documents such as the syllabus, lab exercises, literature readings, answer to quizzes, review sheets, interesting and appropriate Web sites, etc. through

here.

Course Requirements Three lecture-lab exams 300 or 300 or 250

Evaluation, and Grading: ( 100 point each)

Comprehensive Final Exam 150 or 100 or 150

9 quizzes/problem sets

(@12.5 pts each)

Current Literature Review

2 times @ 25 pts each

Laboratory (lab reports)

50 or 100 or

50 or 50 or

100

50

2 times @ 25 pts each 50 or 50 or 50

Micro. Research Paper, Web Site

& Oral Presentation

Total

50 50 50

650 or 650 or 650

Final grades will be determined from the percentage of the total possible points achieved on the basis of: A = 100-90%; B = 89-80%; C = 79-70%;

D = 69-60%; F = 59-0%. Any student who is dissatisfied with grades or receives failing grades during this course is urged to discuss this with the instructor. Exam 1: Probably the Week of 9/23; Exam 2: Probably the

Week of 10/14; Exam 3: Probably the Week of 11/11; Final exam:

Definitely Wednesday, December 11, 2:00 - 4:00 PM, BG 109; 33-50% comprehensive on lab and lecture material covered on Exams 1 and 2; 50-

67% on lab and lecture material covered from Exam 2 to end).

Lecture/Lab Exams: Exams can consist of any combination of True/False, fill-in-the-blank, multiple choice, matching, short answers, problems, diagrams, and short essays on what was covered in lecture and lab (85-90 pts lecture, 10-15 pts lab for hour exams; 120-130 pts lecture, 20-30 pts lab for the comprehensive final exam). I usually include bonus questions (5 - 10% of exam value) for extra points. Review sheets will be given out prior to each exam. Exams will usually be given during the first part of labs.

Quizzes/Problem sets: There will be 9 quizzes/problem sets given during the Labs, each counting

12.5 points with, perhaps, a bonus point for the quizzes. One of the 9, presumably the lowest grades or missed ones, will be dropped for a total of up to 100 points. For this reason, there will be no make-up on quizzes. You will receive a “0” for any missed quizzes for any reason. It is strongly encouraged that you take all quizzes and not intentionally miss early ones in case you need to miss one later in the term. Each quiz will be about 10 minutes in length and given at the beginning of the lecture or lab. The

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quizzes will cover the previous 1 or 2 reading assignments AND what was covered in lecture on the previous 1 or 2 lecture days OR will cover the previous 1 or 2 lab(s) AND what will be done in the lab that day. Questions will be similar to lecture exam questions. Problem sets will be due 2-7 days after assignment by 5 PM on the indicated day. Problem sets will be worth up to 6.25 points any time after the 2-7 days until 5 PM on the last day of class. You can expect a quiz/problem set about every week or so. See below for how these 100 points will/can be used in your final point total.

Grading Option: You can deduct 1 quiz or problem set grade of the 9 total for a total of 8 quizzes/problem sets worth up to 100 points. One-half of these points

(up to 50 points) will be included in your point total. The remaining one-half, up to 50 points, can be used in one of three options,

Depending on the individual grades of your three lecture/lab exams or final exam:

1.

The remaining one-half of your quizzes/problem sets total grade, up to 50 points, is discarded. You will keep your 3 lecture/lab exam grades and final exam grade. OR

2.

Keep the remaining one-half of your quizzes/problem sets total grade, up to 50 points, and add it to 1/2 of your lowest hour lecture/lab exam grade (the other 1/2 is discarded). You will keep the remaining two lecture/lab exam grades and final exam grade will be counted as is. OR

3. Keep the remaining one-half of your quizzes/problem sets total grade, up to 50 points, and add it to 2/3 of your final exam grade (the other

1/3 is discarded). You will keep your 3 lecture/lab exam grades.

Make-Up Exams: NO Make-Up quizzes will be given for the quizzes. If you miss quizzes for any reason, up to two of these missed quizzes will be dropped from the 12 possible quizzes and problem sets; the rest of the missed quizzes will be awarded 0s. It is strongly encouraged that you take all quizzes and not intentionally miss an early quiz in case you need to miss one later in the term.

Lecture Make-Up exams will be only given after presenting a legitimate excuse to me as discussed in “Attendance Policy”, page 53 in the 2001-2002

General Catalogue. Legitimate excuses include: Medical (requires doctor's note specifying that the severity of illness requires absence from class); death in immediate family (requires copy of obituary announcement from newspaper);official university functions as listed in the Weekly Report; or memo from an instructor for UWA business such as field trips, meetings, etc.

The Dean of CNSM has final authority over excused absences.

Planned doctor/dentist/etc. appointments are not approved excuses! Make these appointments for before or after the class lecture or exam . If an absent is approved, Make-Up exams (you don’t want to take my make-up exam!) must

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be taken within 7 days from the original exam date. Other options are possible.

Current Literature Review: Each student will prepare two literature reviews on various Biochemistry publications @ 25 points each. Each review will be due at 5 PM between exam I and II (Friday, probably October 4) and exam II and III (Friday, probably November 8). I will provide the format in the near future. For each review, I will put one copy of two - three Microbiology publication on reserve in the library to be only used in the library or on the course web site.

Pick one to do your review. Feel free to make your own copy. Each literature review will be worth only 12.5 points any time after the due date and 0 points if not turned in by 5 PM on the last day of class,

December 5. Typewritten reports are required (computer labs are available in

Bibb Graves, Wallace, and the Sub at convenient hours including some evenings and weekends); Get computer literate now!

Laboratory : Lab Reports: For each completed lab exercises, a Lab report will be written using directions to be given to you at a later time. In real situations, your lab notebook would be a bound book in which every experiment is written up and all data, materials, methods are recorded. For this lab however, obtain a loose leaf binder and set it up according to the directions in the handout to be given out later. The sections of the lab report (see later) should be hand written, legible, according to the guidelines on the handout to be given out later. Include answers to the various questions (all answered of course!) of each lab exercise in your Result section. Lab reports for completed exercises will be turned in 2 times during the course, 25 points each: 5 PM on a Friday between Exam II and III (probably 10/25) and 9 AM on the Thursday following the final exam (12/12 ). Late lab report turn-ins after the first deadline will be worth only 1/2 the points (12.5 pts) and 0 points if not turned in with the last lab report on December 10.

Please be neat and organized; if I can’t read it or find it, you won’t get credit!

Absences: Student will usually work in pairs during each laboratory. Since missed lab work usually cannot be made up, if you must miss or be very late for a lab, you must obtain the details and results of the lab from your partner or see me to see if the lab can be done outside lab hours. Don’t make a habit of this option! Pick a good partner, communicate with your partner, swap phone numbers, and work out the details! One lab absence will not result in any penalty. Because the laboratory is a very important part of the entire course, in addition to loosing attendance points (see below), two or more lab absences, regardless of the reason, will cost you points. Two absences will result in a lowering of your course grade points by 5 points, three by 10 points, etc. on up (see Attendance Policy below). NOTE: (1) The laboratory may require some additional time outside the assigned time for the regular lab .

(2) Lecture/lab exams will probably be given during the first 90 minutes

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of three of the laboratories. (3) Depending on what we will be doing in the lab, a portion of the lab time may also be used for lecturing and problem solving.

Make-up Labs: There are no make-up labs. If you miss a lab or labs, it is your responsibility to get the information from your partner or lab mates.

Obviously it is very important for you to choose a good partner! However, if you see that you cannot make your laboratory section on Thursday, there is a possibility that you can attend the earlier or later laboratory that day IF approved IN ADVANCE by the instructor. Don’t make this a habit!

Micro Reseach Paper, Web

Each student will prepare a paper and construct a web page link to the

Link, & Oral Presentation

course Web site on some aspect of Microbiology. I will provide a list of topics and guidelines for the paper and web page link at a later time. In addition, each student will prepare a 10 min. oral presentation on their topic.

Due dates will be given later.

Extra Credit: Homework (Optional): Homework (up to 12 extra points added to your total points which could result in up to about 2 percentage points on your final grade): ):

On single stapled pages, answer the odd-numbered (1, 3, etc.) of the Multiple-Choice Questions, Concept Questions and Critical-

Thinking Questions at the end of chapter or part of chapter covered during the lectures (only those questions pertaining to what was covered). For the multiple-choice questions, give one reason why one of the other choices was not the correct answer. Homework will be collected at the beginning of the each of the three hour exams and the final exam (NO TIME EXTENSIONS

WILL BE GRANTED). You may choose to do this at any time for partial credit (only in up to 3 point increments) from one exam to the next; however in order to get up to the maximum 12 points, you must turn in your question journal at the indicated time, starting with exam 1 and continuing with exam

2, then exam 3 and the final exam. You CANNOT go back and pick up previous ones.

Absence Policy: Attendance will be taken.

Please find a seat in lecture and laboratory that you will use for the whole course. See "Attendance Policy", page 53, in the

2002 - 2003 General Catalogue for details. No credit for the course will be given if the student ".. does not attend at least two-thirds of the class meetings, regardless of the reason for the absences". For this course: 38 of the 58 lecture and laboratory meetings = 2/3. First two total absences (lab and lecture) are free. In addition, my policy is to give 2 points to your total points

(up to 10 points) for each day not absent under 7 total lecture and lab days and deduct 2 points from your total points (the sky is the limit!) for each absence over 7 total lecture and lab days regardless of the reasons for the absences. You keep up with your absences. Note additional penalty points for missing laboratory days.

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Assessment Day:

As a part of the University’s plan to assess institutional effectiveness, a day is set aside each semester (except Summer Semester) for assessment activities.

Although no day classes meet on this designated day, for fall – Tuesday,

October 22, 2002, students are required to participate in assessment activities when they are called upon to do so.

Disability: The University of West Alabama strives to make its programs accessible to qualified persons defined as disabled under Section 504 of the Rehabilitation

Act of 1973 and the Americans with Disabilities Act. Students who have special needs that require accommodation are responsible for notifying instructors in each course in which they are enrolled and appropriate staff members, who in turn will refer the student to the ADA Compliance

Coordinator. Following verification of the student’s status, the ADA

Compliance Coordinator will work with the instructor or staff member in implementing an appropriate plan for accommodation of the student’s needs.

Support documentation of special needs from a physician or other qualified professional will be required if deemed necessary. For additional information, see page 13 in the 2002-2003 General Catalogue; students should also contact the Student Success Center, Foust Hall Room 7, (205)

652-3651, or the Office of Student Life, Webb Hall, Room 311, (205) 652-

3581.

Content/Schedule Change: The lecture and laboratory schedules reflect expected class progress in course subject material and are considered tentative. The lecture and laboratory schedules are subject to change in content and scope at the instructor's discretion. The instructor will make corrections as needed and will announce changes in the class. In some lectures, only selected parts of chapters will be presented and discussed; page numbers will be given out in class. Parts of some chapters may be discussed in lab.

Honesty/Integrity: The UWA Academic Dishonesty policy as stated in the 2002-2003 General

Catalogue, pp. 53-54, and the student handbook will be followed in this course. You may work with other students to complete laboratory exercise questions and problems, laboratory reports, literature reviews, research papers, homework, and problem sets, but you must turn in your own work in your own style of writing. Plagiarism in any of these will get all involved parties "0" for the work and a verbal tongue lashing from yours truly in addition to what is spelled out in the catalogue, pp. 53-54!

Other Policies: Audible Cell Phones, Beepers, and Alarm Clocks will be turned off during lectures and laboratories. Cell phones will not be used during the lectures or laboratories. Food, Drink and Tobacco of any kind are not permitted during lectures (note new carpet, desks and chairs!) or laboratories.

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BY 340 Tentative Microbiology Lecture Schedule

6

7

8

9

2

3

4

5

Week

1

Date

Aug. 19

Aug. 26

Sept. 2

Sept. 9

Sept. 16

Sept. 23

Sept. 30

Oct. 7

Oct. 14

Topic

Course Logistics

Microbial Survey and Characteristics

Molecules and Macromolecules

Molecules and Macromolecules

Prokaryotes and Eukaryotes

Prokaryotes and Eukaryotes

No class on Sept. 2

Prokaryotes and Eukaryotes

Prokaryotes and Eukaryotes

Viruses

Exam 1 during lab

Growth and nutrition; metabolism

Lit. Review 1 Due

Growth and nutrition; metabolism

Informational Molecules, Genetics

Exam 2 During lab

10

11

12

Oct. 21

Oct. 28

Nov. 4

Informational Molecules, Genetics

Biotechnology

Lab report I due, 10/25

Physical and Chemical Control of Growth

Physical and Chemical Control of Growth

Lit. Review 2 due Nov. 8

13

14

15.

16.

Nov. 11

Nov. 18

Nov. 25

Dec. 2

Drugs, Chemotherapy

Exam 3 during lab

Drugs, Chemotherapy

No classes/labs on Nov. 25, 27, 28, 29

Catch up

Final Exam: Wednesday, December 11, 2:00 - 4:00 PM, BG 109

3, 4, 5

3, 4, 5

6

7, 8

7, 8

9

6

10

11

11

12

12

Chapters

Handout

1

2

2

3, 4, 5

3, 4, 5

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BY 340 Tentative Microbiology Lab Schedule

4

5

6

7

8

9

3

2

Week

1

10

11

12

13

14

15

16

Date

Sept. 9

Sept. 16

Sept. 23

Sept. 30

Oct. 7

Oct. 14

Aug. 19

Aug. 28

Sept. 2

Oct. 21

Oct. 28

Nov. 4

Nov. 11

Nov. 18

Nov. 25

Dec. 2

Topic Exercise Nos.

Lab Safety v, vi

Lab Techniques; metric system

Mini- Winogradsky Column

“, Complex, Minimal, Differential Media

Preparation; pH

Environmental Surveillance

Handout, Appendix I

Handout

Handout

12, 15, 3

50, 51

1, 2 Microscopy, sizes

No class on Sept. 2

Pure Culture Techniques, simple staining

Specialty Stains; motility

4, 6

7, 8, 9

10 Eukaryotes

Exam 1 during lab viruses, bacterial population counts

Bacterial Examination of Water

Exam 2 During lab

34, 5, 31, 35, 36 spectrophotometry 13

Growth, Effect of Environmental Conditions 14, 22, 23, 24

33

Antiseptics and Disinfectants

Antibiotic Susceptibility

Intracellular and Extracellular enzymes

Biochemical reactions mutations finish labs, work on unknown

Exam 3 during lab transformation, DNA preparation

No classes/labs on Nov. 25, 27, 28, 29

PCR

25, 26

27, 28

16, 17

18, 19, 20

54, 29

Previous

55, Handout

Handout

Final Exam: Wednesday, December 11, 2:00 - 4:00 PM, BG 109

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Strategies for Success in BY 340, Microbiology

The course content of BY 340 may be totally new, vaguely familiar, or a more in-depth analysis of similar material from other courses and experiences. Regardless, there are several strategies that I feel you can employ to master and retain the material, relieve stress and, actually, benefit you and your educational objectives. The following DOs and

DON'Ts are intended as helpful hints - clearly not all are helpful to all students.

DOs

1. DO attend ALL lectures and labs and DO be on time!

2. DO take notes on lecture and laboratory materials (these areas will be emphasized on exams and quizzes/problem sets).

3. DO read all assigned reading material, preferably before the lecture and laboratory are given.

4. DO take notes from the assigned readings, either add to lecture notes or use to clarify lecture notes. One helpful technique is to re-copy your notes and add to them from the assigned readings. Another is to divide you notetaking paper in half; on one half, record your notes from lecture, on the second half, add to the notes from your classmates, or from your textbook readings.

5. DO review lecture and laboratory notes/assigned readings frequently - generally, 1 hour lecture requires 2 to 3 hours of outside review.

6. DO form study groups: Find 3 to 5 friends or classmates in BY 340 and meet weekly to review/discuss material.

Make sure that this is a real study group and not a social one!

7. DO review the questions and answers when we go over the quizzes and exams. By determining the type(s) of questions you answer incorrectly, you can modify your study habits to overcome deficiencies.

8. DO participate in the Homework for Extra Credit. This will force you to keep up with the assigned readings by regularly doing the questions. Go ahead and answer the odd number questions too. If you do not want to do the

Question Journal, DO ALL of the questions anyway. You will probably see these questions again!

9. DO ask questions at the end of the class lecture or lab. DO assume that other students probably have the same question(s).

10. DO meet with me during office hours if you have further questions or other things to discuss.

11. If you miss a lecture, DO communicate with your classmates about what was covered, the reading assignment(s), and whether there were any handouts. That is your responsibility.

12. DO integrate material from various lectures and assigned readings to develop your understanding and appreciation of the BIG PICTURE.

13. DO be an active learner; my role is as a facilitator and I cannot learn the material for you.

DON'Ts

1. DON'T miss lectures and laboratories, DON'T come to lectures and laboratories late, and DON'T leave lectures and laboratories early. If you have to be late or leave early, please notify me before class. DON'T be rude and inconsiderate to the other class members and lab partners! And DON’T make it a habit.

2. DON'T sleep, read newspapers or talk during lectures and laboratories If the class is too easy for you and you are bored, I can find supplemental reading material for you or you can just leave.

3. DON'T get behind ( a large amount of material is covered in the course).

4. DON'T memorize lectures independent of each other. See the relatedness of the topics.

5. DON'T cram for exams. Nothing substitutes for studying the subject every day.

6. DON'T cheat on the quizzes/problem sets, lecture exams, laboratory reports, and question journal.

7. DON'T blame me, the instructor, for giving you a poor or unacceptable grade; you EARN your grade!

8. DON’T have Cell Phones, Beepers, and Alarm Clocks turned on during lectures and laboratories. 9. DON’T use

Cell phones during the lectures or laboratories.

10. DON’T use Food, drink, and Tobacco of any kind during lectures or laboratories.

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Student Profile (BY 340 Microbiology)

Name:

Local Address:

Phone No. (where I can reach you if needed):

Student No.:

Hometown and High School: email addresses (ever student should have a cp account and, perhaps, a second one: Give both!)

Major:

Career Goals:

Minor:

1. What other Biology courses are you taking or have taken at UWA and when?

2. What other Science and Math courses are you taking or have taken at UWA and when? Give number and name.

Chemistry

Physics

Math

Other (please list)

3. Please list Science and Math Courses by name that you have taken at other colleges and when.

Biology

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Chemistry

Physics

Math

Other (please list)

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