Microscopy: Parts & Observations 2009 Name: _TEACHER'S NOTES_ Period: ____ 1. What are the parts? Label the parts of a microscope on the diagram below. Eyepiece Body tube Nosepiece Arm Low power Med. Power High power Stage Stage clips Coarse Adj. Diaphragm Fine Adj. Light source Base WORD BANK Arm Eyepiece Nosepiece Base Fine adjustment Stage Body tube High power objective Stage clips Coarse adjustment Light source Med. Power objective Diaphragm Low power objective 2. What do the parts do? Match the part of the microscope with its function. 1 Minority Science Programs – School of Biological Sciences – University of California, Irvine Microscopy: Parts & Observations 2009 _5_ Where the slide is placed 1. Objectives _4_ Used to focus when using the low power objective 2. Base _1_ Magnifies the specimen 3. Arm _3_ Used to carry microscope 4. Course adjustment _7_ Used to focus when using the high power objective 5. Stage _6_ The lens you look through, magnifies the specimen 6. Eyepiece _2_ Supports the microscope 7. Fine adjustment 3. Magnification Calculate the total magnification for each objective. Eyepiece: 10X Objectives: 4X, 10X, or 40X 4X objective: __10__ x __4__ = __40X__ 10X objective: __10__ x __10__ = __100X__ 40X objective: __10__ x __40__ = __400X__ 4. Letter “e” Observe the letter “e” with your naked eye and the microscope. Draw only what you see through the eyepiece. Include the correct magnification. Naked eye _______ X What do you observe about the orientation and direction of the image? ___In the eyepiece, the “e” appears to be upside-down and backwards.__ 2 Minority Science Programs – School of Biological Sciences – University of California, Irvine Microscopy: Parts & Observations 2009 5. Comic strip Observe the colored comic strip with your naked eye and the microscope. Draw only what you see through the eyepiece. Include the correct magnification. Naked eye _______ X 6. Prepared Slides Observe a prepared slide at different magnifications. Record the title of the slide. Draw only what you see through the eyepiece. Include the correct magnification. Slide title: __________________________ _______ X _______ X Slide Preparations: 3 Minority Science Programs – School of Biological Sciences – University of California, Irvine Microscopy: Parts & Observations 2009 Letter “e”: Make a class set of letter “e” slides by printing out a page of tab-delimited e’s. Cut out each letter and secure them to slides using clear nail polish and a cover slip. Let dry overnight before using. Comic strip: Cut out small pieces of colored newspaper comic strips. Have students place them on a slide with a cover slip when viewing under the microscope. Prepared slides: Use a commercially available set of assorted prepared slides. Cheek cells: Have each student scrape the inside of their cheek using a cotton swab. Smear the swab on a clean slide to dislodge the cells. Add a drop of bromothymol blue stain and cover with a cover slip. Disinfect used slides with bleach. Only allow students to handle their own cheek cell slides. 4 Minority Science Programs – School of Biological Sciences – University of California, Irvine