Name: __________________________________________________
Period: _________
AP Biology Invest. Lab 8: Biotechnology: Bacterial Transformation
Molecular Biology Homework
Directions: Go to http://www.phschool.com/science/biology_place/labbench/lab6/intro.html and
answer the following questions in your lab notebook. You do not
need to rewrite the questions! Make sure to answer using complete
sentences.
1) What is the purpose of this lab?
2) Click next. What is genetic transformation?
3) What bacteria become genetically transformed in this lab? How?
4) Click Next Concept. What is the E.Coli grown on?
5) What is in a colony of bacteria?
6) Click on Next Concept. Why are E.Coli used in this lab?
7) Click on Next Concept. What is a plasmid?
8) What is the purpose of the ampR gene? How can you make –ampR bacteria become an +ampR
bacteria?
9) Click on Next Concept. How do you make a cell competent to take up a foreign piece of DNA?
10) Click on Next Concept. Describe three (3) rules to making/keeping a sterile environment?
Name: __________________________________________________
Period: _________
11) Click on Next. Describe the 5 steps to Bacterial Transformation (Hint: Use the closer look if you need
help).
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12) Do the Activity titled “Place the Stages of Transformation in Order.” Record your answers below.
Answer:
13) Click on Next. Explain these results.
Explanation:
Name: __________________________________________________
Period: _________
14) Click on Next. Do the Activity titled “Label the Results of your Experiment.” Record your answers
below.
15) Click on Next. Take the Self-Quiz. Record your answers below.
16) Click on Next. This is the start of the Lab Part II: Electrophoresis. Describe the background to
restriction enzymes/endonucleases.
17) Click on Next Concept. What does ‘palindromic’ mean?
18) Click on Next Concept. What do you do after adding the restriction enzyme to the DNA?
19) Click on Next Concept. What is gel electrophoresis? Why does this work on DNA?
20) Click on Next Concept. Describe the 6 steps involved in gel electrophoresis:
21) Click on Next Concept. How do you prepare the gel?
Name: __________________________________________________
Period: _________
22) Click on Next Concept. What is the purpose of adding a dye to the DNA sample?
23) Click on Next Concept. Click on Next Concept. When do you turn off the apparatus?
24) Click on Next Concept. Why must you stain the gel with methylene blue?
25) Click on Next Concept. What has the DNA been cut with already? Why is this done?
26) Click on Next Concept. Do the exercise titled “Making a Standard Curve.” Record your answers
below.
27) Click on Next Concept. Why do you make a standard curve?
28) Click on Next Concept. Do the Practice Problem 1. Record your answers below.
29) Click on Next Concept. Do the
Practice Problem 2. Record your
answers.