A crime scene This DNA profile shows a hypothetical example of how gene probes are used in forensic investigations. In this case a victim was murdered. There are three suspects under investigation. A blood sample was taken from the scene of the crime. The blood is believed to belong to the attacker. Two fluorescent probes were used to produce a DNA profile. Adapted from an activity on p98 of Microbiology and Biotechnology in the Biology Modular Workbook series. Authors: Greenwood, Shepherd, Allan, Butler. Published in 2006 by Biozone International Ltd. Reproduced with the permission of Biozone International Ltd Using the profile, answer the following questions. 1. Identify which of the three suspects was the likely killer. 2. What information from the profile helped you arrive at your answer? 3. Why would a sample be taken from the victim and analysed? © WestOne Services 2010 SCIENCE1437 1 4 Until DNA profiling was developed, blood types and fingerprints were used as evidence to assist in the solving of crimes. Why is DNA profiling a more reliable technique? Scroll down for answers. © WestOne Services 2010 SCIENCE1437 2 A crime scene – Answers 1. Suspect 1. 2. The fluorescent bands on the profile from Suspect 1 and the evidence matched. 3. To ensure the evidence was clearly a different DNA to the victim’s DNA. 4 This technique is based on very specific molecular information. The chance of two people sharing similar microsatellites is approximately one in a billion, so identification is very reliable. © WestOne Services 2010 SCIENCE1437 3