Government of West Bengal DIRECTORATE OF ANIMAL RESOURCES & ANIMAL HEALTH Prani Sampad Bhavan, 2nd floor, LB-2, Sector-III, Salt Lake City, Kolkata-700 098 Ph: 2335-1145 Fax: 2335-1187 E-mail: dahvswb@rediffmail.com Website: www.darah.org NOTICE INVITING E-TENDER (2nd call) FOR RATE CONTRACT LABORATORY INSTRUMENTS, LAB. REAGENTS, LAB. KITS & LAB. MOLECULAR REAGENTS (Submission of bid through NIC e-tender portal http://www.wbtenders.gov.in) NIT # WBARD/DARAH/LAB INST. Etc./NIT-03/2P-105(A)/11/15-16 Date of Issue: 5th May, 2015 The Director of Animal Husbandry & Veterinary Services, West Bengal on behalf of the Governor to the State of West Bengal is going for e-tender (2nd call) for preparing the rate schedule of Laboratory instruments for the year 2015-16. On-line tenders are invited only from MANUFACTURERS/AUTHORISED DISTRIBUTORS/DEALERS for rate contract of Laboratory Instruments & Reagents etc. to the following decentralized units under this Directorate. 1. Office of the Dy. Director, A.R.D. & Parisad Officer, South 24 Paraganas (Alipore), North 24 Paraganas (Barasat), Howrah, Purba Medinipur (Tamluk), Dakshin Dinajpur and Jalpaiguri. Central Medical Stores, Sub-depots at Nadia, Bankura, Uttar Dinajpur, Murshidabad, Coochbehar, Siliguri, Bardhaman, Hooghly (Chinsurah), Birbhum, Purulia, Paschim Medinipur and Malda. 2. Office of the Joint Director, A.R.D., Zone-I/II/III/IV Division, P.O. Bardhaman/ Kalyani/Siliguri/Baharampur, Dist. Bardhaman/Nadia/Darjeeling/ Murshidabad. 3. Office of the Joint Director, A.R.D., State Livestock Farm, Kalyani, Nadia / Composite State Animal Husbandry Farm, Salboni, Paschim Medinipur. 4. Office of the Joint Director, A.R.D., Institute of Animal Health & Veterinary Biologicals, 37, Belgachia Road, Kolkata-37. 5. Office of the Addl. Director, A.R.D., Haringhata Farm, P.O. Mohanpur, Dist. Nadia. 6. Office of the Dy. Director, A.R.D., Poultry-Marketing, State Poultry Farm, 42, Graham Road, Tollygunge, Kolkata-40. 7. Office of the District Executive Officer of all district; Station Director-I, Frozen Semen Bull Station, Haringhata/Salboni/Beldanga; Deputy Project Director, Bull Mother Farm, Haringhata; Office-in-Charge, Bull Mother Farm, Salboni all under Paschim Banga Go-Sampad Bikash Sanstha (A Govt. of West Bengal Organization). Laboratory instruments etc., as per catalogue enclosed, are classified in the following groups: Sl. No. Name of the Instrument Acc. Unit Group-A- Laboratory Instruments 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. Laminar Flow(Horizontal) Small autoclave vertical Fully automatic vertical autoclave, GMP model Bio Safety Cabinet Class- II Horizontal High pressure Cylindrical Stem Sterilizer (Autoclave- Semi-automatic: Single door) One chamber CO2 Incubator with work chamber volume approx. 300 litres Refrigerator with data logger (Vertical) Horizontal Low Temp. Freezer(-60°C) Inverted Microscope Refrigerated Micro centrifuge Micro Centrifuge for PCR plate Temperature Indicator with alarm devices and thermal data loggers Manual Rotary Microtome Ordinary Centrifuge Machine Tissue Homogenizer Automatic Rotary Microtome UPS 15 KVA on-line uninterrupted Power Supply Gr. A/01 Pc./01 Gr. A/01 Pc./02 Gr. A/01 Pc./ 03 Gr. A/01 Pc./04 Gr. A/01 Pc./ 05 Gr. A/01 Pc./ 06 Gr. A/01 Pc./07 Gr. A/01 Pc./ 08 Gr. A/01 Pc./ 09 Gr. A/01 Pc./ 10 Gr. A/01 Pc./ 11 Gr. A/01 Pc./ 12 Gr. A/01 Pc./ 13 Gr. A/01 Pc./ 14 Gr. A/01 Pc./ 15 Gr. A/01 Pc./16 Gr. A/01 Pc./ 17 Contd. Page-2 Page-2 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Battery for UPS- 15 KVA on line Gr. A/01 Pc./18 UPS(Uninterrupted Power Supply) 5 KVA on line Gr. A/01 Pc./19 Battery for UPS- 5 KVA on line Gr. A/01 Pc./20 Pilot Freeze Dryer Gr. A/01 Pc./21 Clean room clogs with cuffs Gr. A/01 Pc./22 Programmable peristaltic pump Gr. A/01 Pc./23 Membrane pump Gr. A/01 Pc./24 Ready to connect single use filtration unit Gr. A/01 Pc./25 Door Interlock Switches Gr. A/01 Pc./26 Multichannel Pipette Gr. A/01 Pc./27 Multichannel Pipette Gr. A/01 Pc./28 Thermo cycler with three independent blocks Gr. A/01 Pc./29 Automated DNA/RNA extraction system Gr. A/01 Pc./30 Portable gel electrophoresis system with real time transilluminator Gr. A/01 Pc./31 Computer controlled rapid Microbial Identification system Gr. A/01 Pc./32 Binocular microscope Gr. A/01 Pc./33 Automatic Tissue Processor Gr. A/01 Pc./34 UV-VIS Spectrophotometer Gr. A/01 Pc./35 Gr. A/01 Pc./36 Ampoule Freeze Dryer Research adjustable volume pipettes, Autoclavable with tip box Gr. A/01 Pc./37 Gr. A/01 Pc./38 Research adjustable volume pipettes, Autoclavable with tip box Gr. A/01 Pc./39 Research adjustable volume pipettes, Autoclavable with tip box PCR Plate centrifuge Gr. A/01 Pc./40 Group-B: Laboratory Reagents & Other Glass/ Plastic materials Polypropylene bottle Autoclavable Gr. B/Lab. Regt./100 Pc./01 Gr. B/Lab. Regt./100 Pc./02 Rubber stopper for Polypropylene bottle Gr. B/Lab. Regt./100 Pc./03 Aluminum cap (20 mm diam.), Gr. B/Lab. Regt./360 pcs./04 Neutral Glass vial Neoprene Rubber cap for Neutral Glass vial 3 ml. capacity, 13 mmOD Gr. B/Lab. Regt./2000 pcs./05 Aluminium caps, coloured and non colour for Neutral Glass vial Gr. B/Lab. Regt./5000pcs./06 Gr. B/Lab. Regt./10 pc./07 Gel pack(Ice cold pack) Gr. B/Lab. Regt./50 pc./08 Syringe Filter (Membrane Filter) Gel pack(Ice cold pack) Gr. B/Lab. Regt./10 pc./09 Tissue Culture (TC)Flask with filter cap Gr. B/Lab. Regt./18 pc./10 Tissue Culture (TC)Flask Gr. B/Lab. Regt./18 pc./11 Thioglycollate Broth(BQ vaccine Media) Gr. B/Lab. Regt./01 pc./12 Standard Nutrient Broth (HS Vaccine Media) Gr. B/Lab. Regt./01 pc./13 Formaldehyde 37% (formalin) Gr. B/Lab. Regt./01 pc./14 Gr. B/Lab. Regt./01 pc./15 Glycerol-98% Purified Aluminum Hydroxide Gel (3 % W/V, Liquid type) pH-7 Gr. B/Lab. Regt./01 pc./16 Yeast Extract Powder Gr. B/Lab. Regt./01 pc./17 EMEM Gr. B/Lab. Regt./10 bot./18 L-Glutamine Gr. B/Lab. Regt./10 vial/19 Group – C : Laboratory Kits Classical Swine Fever Antigen ELISA test kits Gr. C/Lab. Kit./1kit/1 M. bovis Antibody ELISA test kit Gr. C/Lab. Kit./5kit/2 Bacillus anthracis (common)PCR kit Gr. C/Lab. Kit./1kit/3 RT PCR kit Gr. C/Lab. Kit./1kit/4 PRRS Antibody ELISA test kit Gr. C/Lab. Kit./1 kit/5 Bovine Herpes virus(BHV-1) real time PCR kit Gr. C/Lab. Kit./150 tests/ 6 Bovine viral Diarrhoea virus (BVD) real time RT PCR Kit Gr. C/Lab. Kit./150 tests/ 7 Quantitative(q) PCR mastermix kit Gr. C/Lab. Kit./ 100 Reaction/Kits/ 8 Quantitative(q)RT PCR master mix kit Gr. C/Lab. Kit./ 100 Reaction/Kits/ 9 DNA purification kit for whole blood and tissue Gr. C/Lab. Kit./ 250 purification / 1 kit /10 Viral RNA purification kit Gr. C/Lab. Kit./ 50 purification /1kits/11 Rapid AIV Antigen Test Kit Gr. C/Lab. Kit /30 tests/pack/12 Contd. Page-3 Page-3 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25 26. 27. 28. 29. 30. 31. 32. 33. 1. 2. 3. 4. 5. IBD Virus Antigen Test Kit Gr. C/Lab. Kit /10 tests/kit/13 NDV ELISA Kit (Ab. Detection) Gr. C/Lab. Kit /480tests/pack/14 Rapid IBD test kit Gr. C/Lab. Kit /10 tests/pack/15 Rapid NDV Antigen Test kit Gr. C/Lab. Kit /10 tests/pack/16 Sandwitch ELISA test Kit for BSE Purification of PrPsc. Gr. C/Lab. Kit /192 tests/Kit/17 Avian Influenza Virus subtype (H5) Real time kits Gr. C/Lab. Kit /150 tests/pack/18 Avian Influenza Virus subtype (H7) Real time kits Gr. C/Lab. Kit /150 tests/pack/19 Avian Influenza Virus subtype (H9) Real time kits Gr. C/Lab. Kit / 150 reactions/Kit /20 AIV antibody ELISA Kits Gr. C/Lab. Kit /480tests/Kit/21 Rapid AIV subtype H5 antigen detection kit Gr. C/Lab. Kit /25tests Kit /pack/22 Avian Influenza Virus subtype (H5) Antibody detection kits Gr. C/Lab. Kit /192tests/Kit/23 Avian Influenza Virus subtype (H7) Antibody detection kits Gr. C/Lab. Kit /192tests/Kit/24 Newcastle Disease (ND) Virus Real time kits Gr. C/Lab. Kit /150 tests/pack/25 Avian Infectious Bronchitis Virus, Real Time Kits Gr. C/Lab. Kit /150 tests/pack/26 Avian Adenovirus(Egg Drop Syndrome) Gr. C/Lab. Kit /150 tests/pack/27 Mycoplasma gallisepticum Real Time Kits Gr. C/Lab. Kit /150 tests/pack/28 Brucella genus Real Time kits Gr. C/Lab. Kit /150 tests/pack/29 Camylobacter foetus Real Time Kits Gr. C/Lab. Kit /150 tests/pack/30 Salmonella detection PCR kits Gr. C/Lab. Kit /25 runs/kit/31 E coli 0157:H7 detection PCR kit Gr. C/Lab. Kit /25 reactions/Kit/32 SYBR Green I PCR kits Gr. C/Lab. Kit /200 runs/33 Group- D: Laboratory Molecular Reagents Basic synthesis(Per base charges) for Primers Gr. D/Lab. Regt./1 vial/1 Probe Gr. D/Lab. Regt./1vial/2 Probe Gr. D/Lab. Regt./1 vial /3 Primer, forward Gr. D/Lab. Regt./ 1 vial /4 Primer, Reverse Gr. D/Lab. Regt./1 vial/5 N.B. *Catalogue of Laboratory instruments along with specification and packing unit is attached. 1. Uniform rate must be quoted applicable to the entire aforementioned units. Quoting of separate rate against each of the above units will not be considered for acceptance. 2. Offered rate should remain valid for a period OF ONE YEAR FROM THE DATE OF ACCEPTANCE OF THE TENDER and any extended period, if situation arises. 3. Necessary Earnest Money Deposit or valid exemption certificate along with mandatory documents in Statutory & non-statutory cover are to be uploaded for participation in the tender. 4. After publication of the N.I.T. in the newspapers, detailed catalogue along with terms & conditions, BOQ (Bill of Quantities) may be obtained from the Website http://www.wbtenders.gov.in at free of cost. 5. Tenders will be floated in two parts – one Technical Bid for technical proposal (mandatory documents in Statutory & non-statutory cover) and other for Financial Bid for quoting rate & VAT (whole amount) in BOQ’s in dedicated column. 6. Submission of on-line tender may be made by the bidder with the help of Digital Signature Certificate (DSC). 7. The software shall make automatic encryption of the technical bid as well as financial bid. No one will be able to open these two bids prior to schedule date & time as fixed by the undersigned. 8. After evaluation of technical bid, those who will technically qualify, their financial bid will be opened for evaluation. 9. Disclosure of rate other than BOQ will disqualify the bidder. Continued on Page-4 Page-4 10. On-line bid submission will start on 12th May, 2015 at 4 pm in the e-tender portal viz. http://wbtenders.gov.in. 11. Earnest Money Deposit should be submitted physically on or before 3rd June, 2015 up to 4 pm at the office of the Director, AH & VS, West Bengal Prani Sampad Bhavan, LB-2, Sector-III, Salt Lake City, Kolkata-700 098. 12. Sample along with Literature of quoted item/s should be submitted physically before sample selection committee on stipulated date, place & time that will be communicated later on. 13. On-line tenders in two parts i.e. Technical Bid (Part-I) & Financial Bid (Part-II) are to be submitted up to 5 pm on 2nd June, 2015 for the aforementioned N.I.T. 14. Technical Bid Opening Date is tentatively fixed at 11 am on 3rd June, 2015. Other activities i.e. date of uploading List of Technically qualified bidders, date of opening of financial bid etc. will be communicated later on. 15. The above schedule is fixed, however, the undersigned reserves the right to change the date of activities in case of any exigencies through a notice in the departmental website viz. www.darahwb.org & Notice Board at above addressed office. The entire tender documents including N.I.T, terms & conditions for submission of tender and BOQs are part & parcel of the tender. The Bidder should abide by all the tender terms & condition and upload the same as a token of acceptance. The undersigned reserves the right to reject and/or cancel all tenders at any stage without assigning any reason thereof and will not bound to accept lowest bid. Sd/(Dr. S. Bose) Director of Animal Husbandry & Veterinary Services, West Bengal CATALOGUE Items for tender list Sl. No. Name of the Instrument Specification with presentation Acc. Unit Pkg. Unit Group-A- Laboratory Instruments 1. Laminar Flow(Horizontal) 2. Small autoclave vertical 3. Fully automatic vertical autoclave, GMP model 4. Bio Safety Class- II Cabinet Horizontal Laminar Flow Clean Air Work Station ,Work Space – 4’ x 2’ x 2’Power Supply – 230V AC 1-ø 50 Hz, 500 Watts HEPA Filter – 1220Work Space – 1186 x 610 x 577 mm Overall Dimension – 1302 x 915 x 1425 mm, Option:- Polycarbonate front door with gas filled hinges for 4 feet. System:HEPA Filter, Pre Filter, Motor blower assembly, Stainless steel 304 Work Table, Glass side panels, U-V light, fluorescent lights (in excess of zoo lux)Air / Gas regulator, Pressure monitor from 0 to 25 mm, Electrical outlet with switch to be supplied with polycarbonate Front door with hinges. Filters:Separator less Minipleat antimicrobial EU-13 HEPA filter with an efficiency of 99.97% on monodisporse,0.3 micron challenge, biocidal anti-microbial prefilter conforming to EU-6 rating. Media is impenitently bactericidal & fungicidal. Performance:- Air Cleanliness: Class M 3.5 (US FED 209E) 150 Class 5 [ISO 14644-1 : 1999(E)] Inner chamber made of SS 304 grade & outer of MS covered with SS sheet. Fitted with pressure gauze steam release valve safety valve and paddle fitting device to work on 220 V AC size of inner chamber 20” x 12” with basket capacity 40 liters. 2 kW SS lid. should be equipped with timer . Fully automatic with digital temperature and time controller( 4 sensors 3 temp + 1 pressure) with printer interface to connect to dot matrix printer, to be supplied with printer, of size 22X 30 ( diameter X height in inches) on site validation service, calibration certificate with IQ, OQ and PQ protocols should be provided with all the accessories including printer, printer paper etc. Motor driven front door, alarm for HEPA filter blockage, air flow indicator, timer for UV light, two no. Indian standard power sockets inside the cabinet, to be provided with UV light, micro processor controlled, pressure sensor monitoring, digital airflow verification,, interior eye level display on rear wall,, performance factor indicator, display parameters including downflow air velocity, cabinet malfunction, time of day, operating time of UV lights, total operating hours, time for delayed start, hand held control for use inside cabinet eliminates glove change with all the accessories including one additional UV light, Inner dimension: 1220 x 610 x 610 mm (L x D x H), DQ,IQ,PQ and OQ documents should be provided, Hour meter for monitoring UV life, certificate regarding installation Gr. A/Lab. Inst./01 Pc./01 cartoon Gr. A/ Lab. Inst./01 Pc./02 Crate Gr. A/ Lab. Inst./01 Pc./ 03 Crate Gr. A/ Lab. Inst./01 Pc./04 cartoon 5. Horizontal High pressure Cylindrical Stem Sterilizer (AutoclaveSemiautomatic: Single door) 6. One chamber CO2 Incubator with work chamber volume approx. 300 litres 7. 8. Refrigerator with data logger (Vertical) Horizontal Low Temp. Freezer (-60°C) 9. Inverted Microscope 10. Refrigerated centrifuge Micro in govt. institutions, one year warranty plus two years additional AMC The inner chamber, steam jacket, outer chamber and boiler are made of heavy gauge stainless steel with leakproof argon are welding. All units are hydraulically tested upto 40 Psi, to work up to 20 Psi. Steam boiler is fitted with water level indicator, pressure gauge, low water cutoff device and automatic pressure control device, working chamber- Diameterx Depth 500 mm x 1200 mm, 221 litres to 235 litres. Air jacketed heating system with temp. Control by microprocessor,Temp. Range from ambient +3 o C to + 55 o C ,Temp. Deviation with respect to time is + 0.1K. CO 2 control by Microprocessor based & by heat resistant Thermal Conductivity ,detector to withstand heat of decontamination System should have built-in automatic decontamination facility to remove bacteria, fungi , spores ,mycoplasma etc. without removing sensor , fan or any other fitting. The system should employ automatic decontamination routine at moist heat of 90 o C or dry heat of 180 deg C Built-in audible and visual water level alarm should be available when the water reservoir needs to be refilled to ensure a constant high level of humidity and to prevent cultures from drying out. Independent electronic over temp. protection with separate sensor should be available for sample protection. Suitable for 230V, single phase 50Hz operation. Suitable servo voltage stabilizer with high voltage low voltage cut off circuit, auto reset with delay timer and spike eliminator etc. & Double stage CO2 pressure regulator with stainless steel diaphragm & 18Kg CO2 Cylinder filled with CO2 gas 99.5% or better purity required for operation of CO 2 Incubator should also be quoted; Warranty 2 years; Optional item : Oxygen control accessory with 3 nos. Interior gas tight split doors should be quoted. 290 – 310 liters 400 lt. capacity, with high and low alarm, to be supplied with CO2 back up system including controller for CO2 back up with CO2 cylinder connector and all the necessary arrangements for protection against power outrage, to be provided with voltage stabilizer, should be operated on 230 V AC,50/60 HZ with all the accessories as mentioned Illumination LED for transmitted light, objective turret 4 position, manual, stage fixed, t be supplied with 6 plan achromatic objectives ,4X,10X,20X,40X,50X, to be supplied with power input jack power switch, USB port, coaxial focusing knob, Illumination wheel, freeze button, save button, with integrated software for adjustable saturation and contrast, color temperature control, to be supplied with all the accessories including 12.1’ high resolution color monitor with adjustable tilt, AC adaptor,, vessel holder for 25 cm2 and 75 cm2 Table top microprocessor controlled refrigerated micro centrifuge with autoclavable 24 x 2 ml rotor bio containment lid for superior safety for infectious materials & higher lid of the rotor suitable to accommodates kits max speed 15000rpm with 10 rpm increment, maxRCF 21380 X g 10 g increment. Temp. Range from - 16◦C to + 40◦ C. Bright large LCD easy to read digital display for speed, time, temp. & RCF. Maintenance free brushless motor drivee. Very fast acceleration and Gr. A/ Lab. Inst./01 Pc./ 05 Box Gr. A/ Lab. Inst./01 Pc./ 06 Cartoon Gr. A/ Lab. Inst./01 Pc./07 Box Gr. A/ Lab. Inst./01 Pc./ 08 Cartoon Gr. A/ Lab. Inst./01 Pc./ 09 Box Gr. A/ Lab. Inst./01 Pc./ 10 Cartoon 11. 12. 13. 14. 15. 16. Micro Centrifuge for PCR plate Temperature Indicator with alarm devices and thermal data loggers Manual Rotary Microtome Ordinary Centrifuge Machine Tissue Homogenizer Automatic Microtome Rotary deceleration around 12 seconds or less should be available. Noise level should be less than 56 dB ( A) . Quick run / pulse run facility should be available. Should be supplied with rotor for PCR strip tubes 8 x 8 with lid; The rotor should be autoclavable; Power: Suitable for 220V±22 V , AC, single phase , 50 Hz/6 Hz operation; 3 Amp Warranty : 2 years. After-sales service : Prompt & efficient after - sales service should be available from Kolkata office, to be supplied with 24 no. adapters for 0.2 ml. micro centrifuge tubes and 24 no. adapters for 0.5 ml. micro centrifuge tubes, Indian standard power cord and all the accessories to run the machine. Micro centrifuge with rotor for 96 well PCR plate, fixed speed for pulse centrifuge of PCR plate Gr. A/ Lab. Inst./01 Pc./ 11 Cartoon Automatic (temperature ranges from 40°C and -200°C to -800°C) Gr. A/ Lab. Inst./01 Pc./ 12 Cartoon Imported microtome with low maintenance & backlash free micrometer feed system with cross bearings for vertical stroke. Section thickness setting from 0.5 to 60mm, thickness increment steps of 0.5/1mm up-to 10mm, specimen retraction -220mm(approx), mechanical trimming function at two steps(preferably 10 & 50mm), vertical specimen stroke length at least 59 mm, horizontal specimen feed approx 28 mm, hand whell lockable in any position using two independent locking systems, specimen orientation facility: 8 deg in X, Y, Z direction, disposable blade holder with lateral displacement facility for complete utilization of the disposable blade edge, 100 pcs compatible disposable blades, accessories neede to run the instrument smoothly, profile ‘C’-16 cm long knife- 2 qty., conventional knife holder should be compatible with system, kolkata based after sale service with proven record RPM up to 5000, Capacity 0f 18 tubes of 15 ml. capacity Gr. A/ Lab. Inst./01 Pc./ 13 Gr. A/ Lab. Inst./01 Pc./ 14 Box Allow homogenization of various samples derived from animal tissue, central nervous system and peripheral tissues, e.g. tonsil, spleen, lymphoid tissue etc for TSE testing, capacity upto 24 grinding tubes per run simultaneously in 45 seconds, 1 predefined programme intended for TSE testing 1 userdefined programme, speed: 5000-6800 rpm, run time 5-90 sec, number of cycles-1-3, display and control panel Backlit LCD ,2lines and 16 characters long /4 button key pad, should be able to use in Bio-safety lable-3 lab.500 samples should be homogenized per hour. Fully Automatic Rotary Microtome should be ergonomically designed. Force compression system should be available for effortless and uniform hand wheel rotation. Section thickness setting upto 100μm, with provision of step- by-step increment. Specimen feed approximate 25 mm(via stepper motor) or above vertical stroke length of 70mn(+/-5%). Specimen retraction up to 90 µm or above with 5 μm increment steps with provisions of On/ OFF. Mechanical trimming (500 μm or above) thickness selection facility with different settings step must be available. Easy alteration should be Gr. A/ Lab. Inst./01 Pc./ 15 Cartoon Gr. A/ Lab. Inst./01 Pc./ 16 Box 17. UPS 15 KVA on-line uninterrupted Power Supply 18. Battery for UPS- 15 KVA on line UPS(Uninterrupted Power Supply) 5 KVA on line 19. 20. 21. Battery for UPS- 5 KVA on line Pilot Freeze Dryer available between trimming and reaction mode. Precise specimen orientation of 8 horizontal and vertical direction. 2 monitorized forward and backward specimen coarse feed speeds must be available (preferably 800 µm/s and 900 µm/s). Disposable knife holder with lateral displacement facility with figure guard in contrasting colour. Han wheel lockable at any position with additional12 O'clock locking by simply pulling the handle for easy changing specimen and or blade. Slot cover to protect the interior of the instrument from sectioning debris. Spacious magnetized section waste tray for collecting section debris .Section thickness totalizer and section counter. Max specimen size 50x 60x 40 mm. High profile blade(at least one pack of 50 blades). Sectioning speed range at least upto 400 mm/s. UPS System should have input Power factor control & low less than 0.5% THD(1) with IGBT rectified technology. Input Voltage: 339-438 VAC. Operating frequency:50/60 Hz.(45-65 Hz.), Input Power factor : > 0.99 Input Current Distortion: 5% THD in normal network Condition. Normal Output Voltage:220/230/240VAC, Single Phase. Output Voltage Regulation:+/- 2% Static, +/-5% Dynamic at 100% load change<1µs response Time, overload capacity 150% for 5 seconds/125% for 1 minute/1000% for 20µsec.(Bypass), out power factor:0.9 i.e., 15 KVA/13.5 KW, DC Bus voltage: Must be 384 Volt, Type of Battery: Sealed Maintenance free Lead Acid Battery. Back Up time: Minimum two hours, Minimum battery AH required: Minimum 24960 VAH & UPS should have advanced Battery Management Features. Efficiency : 92% with rated non-linear load, graphical LCD with Blue Black Light, 4 Led, 1 x RS 232 for local support, 2xX-slot(empty) 1x Relay contact, 1 X emergency power off input, 2 X Environmental input. 384V, Sealed maintenance free battery compatible with 15 KVA UPS Gr. A/ Lab. Inst./01 Pc./ 17 cartoon Gr. A/ Lab. Inst./01 Pc./ 18 Box On line PWM with IGBT as switching device. Input voltage range : 165 – 270 VAC. Nominal voltage out put : 230 VAC Single phase. Out put wave form : Pure. Isolation: Out put isolation transformer should be available as standard . DC Bass voltage must be 18 Volt. Type of battery : Sealed maintenance free lead acid battery. Back up time : min 2 hours, Minimum battery AH required min. 11700 VAH. Gen set capability, UPS should be engine generator compatibility parameters. LCD panel display. Multiple capabilities eliminating user gap. Out power fraction 0.08 min. 12V, 65 AH (Dry Cell); Sealed maintenance free battery UPS Gr. A/ Lab. Inst./01 Pc./ 19 Cartoon Gr. A/ Lab. Inst./01 Pc./ 20 Box Shelf temp control range up to -65'C, lowest shelf temp up to -70'C,lowest condenser temp 85'C,Maximum condenser capacity 35 lt., maximum ice condensing capacity in 24 hours (L) 20, condenser pull down from 20'c to -45'C≤25 min, shelf pull down from 20'C to -40'C ≤30 min,number of compressors 2, refrigerant RS508B R407C, vaccum time to 100 milliTorr ≤20 min, lowest system vacuum ≤15mT(milliTorr),aircooled system with hydraulic stoppering system and 8’ vapour port, 6 nos of freeze drying trays , 3 ml vial capacity> 2500 no., to be supplied with software and work station with following features Software: It utilizes Programmable Logic Controller (PLC) for Windows as per Current Good Manufacturing Practice (c GMP) standards and an Allen-Bradley Micro Logic 1500 Gr. A/ Lab. Inst./01 Pc./ 21 Crate 22. Clean room clogs with cuffs 23. Programmable pump 24. Membrane pump 25. Ready to connect single use peristaltic and regulatory guidelines for computer systems used for pharmaceutical processing equipment. Up to sixteen (16) product temperature-monitoring probes, Time, temperature and vacuum level control for each step of the Primary and Secondary Drying phases, Up to twelve (12) Thermal Treatment (annealing) steps, Integrated performance and vacuum integrity testing routines, Door pre seal routine, Cascade refrigeration handling with inter stage control, Work station specification: Capacitance manometer vacuum gauge in addition to standard Pirani vacuum gauge, Single point eutectic display, Moisture sensor to aid in endpoint determination, Backfill to set point for highly controlled product storage, Up to three (3) levels of security to keep the workstation safe., Pirani/ capacitance manometer differential endpoint determination to aid in product dryness detection, Isolation valve control allowing automated pressure rise testing during Primary Drying Extensive alarming to protect personnel, , product and equipment, Extensive alarming to protect personnel, product and equipment, Remote View option allowing remote monitoring and control of the lyophilizer, Thorough historical data trending with export function, synoptic screen for real time monitoring of the system, Local area network (LAN)-ready., work station to be supplied with the system. Microsoft Windows based, Intel Core 2 Duo Processor, 2 GB RAM, 500 GB Hard Disk Drive, CD/DVD Re-Writable & Floppy Disk Drives, USB Laser jet Printer, 17-Inch Flat Screen Monitor, software along with workstation on which the software will be installed, connecting cable between workstation and lyophilizer, 6 no. vial loading trays with rings, one 15 KVA suitable UPS for power outrage protection, inclusive of all installation charges, including two year warranty and one tear additional AMC, 5 lit. vacuum oil, one tube high quality vacuum grease Made of TPU, ideal for use in clean rooms, holes in shoe and in removable sole for better ventilation, removable sole and detachable cuff, Autoclavable at 121°C. To be supplied with autoclavable silicon tubing 4mm. ID 25 mt. length, Dispensing tube stainless steel, 4 mm. internal diameter 10 cm. length, aspiration tube 4 mm. internal diameter, stainless steel, 10 cm. length, with additional autoclavable silicon tubing 8mm. ID 25 mt. length, Dispensing tube stainless steel, 8 mm. internal diameter 10 cm. length, aspiration tube 8 mm. internal diameter, stainless steel, 10 cm. length, one tube collar with all the accessories as mentioned, along with SS sinker and aspiration tubes, capable of dispensing liquid from 0.5 ml. to 1 litre capacity, programmable for timed operation or manual operation. A self-priming diaphragm pump with an electronic speed regulator, complete with connector tubing set. It pumps aqueous solutions quietly without stressing the product, and is equipped with an overpressure limiter which can be set at 1-3 bar. The pump, stainless steel sinker and tubing are resistant to 1N NaOH., max. operating pressure 3 bar (4.5 psi), preset to 2.5 bar (36.3 psi), pump to be supplied with suitable tubing, diaphragm max 3000/min, rated output for water 650 ml/min without pressure, 300 ml/min with filtration system, materials made of Polypropylene housing, PTFE membrane, EPDM seals and valves, self priming up to 3 m water column, power consumption 0 .75A. 20 cm2 filter area, optimal flow path and automatic venting, surfactant free cellulose acetate Gr. A/ Lab. Inst./01 Pc./ 22 Pack Gr. A/ Lab. Inst./01 Pc./ 23 Cartoon Gr. A/ Lab. Inst./01 Pc./ 24 Box Gr. A/ Lab. Inst./01 Pc./ 25 box filtration unit 26. Door Interlock Switches 27. Multichannel Pipette 28. Multichannel Pipette 29. Thermo cycler with three independent blocks 30. Automated DNA/RNA extraction system 31. Portable gel electrophoresis system with real time transilluminator membrane filter with 0.2 μm pore size, Outlet side with protective filling bell, additional 100% binderfree glass fiber pre filter on the cellulose acetate membrane, luer lock as an inlet connecter, Vent ports on the inlet side of the housing are protected against liquid passage by a hydrophobic PTFE membrane, filtration capacity 1 ml. to max. ten lit., hold u volume before bubble point 1 ml., polycarbonate housing, EO sterilized, filter material PES type, filter diameter 64 mm., Easily and quickly to be installed, advanced design as per clean room concept, maintenance free control panel, to be provided with manual switches for emergency mode, electromagnetic system, to be provided with biometric reader, indicator lights and alarm buzzer, when one door is closed the opposite door will not open 8 channel, 5-50 µl capacity should be suitable for research purpose, to be mentioned in the literature, fully autoclavable and UV resistant, 8 channel, 50-300 µl capacity should be suitable for research purpose, to be mentioned in the literature, fully autoclavable and UV resistant Capable of running three different experiments simultaneously by three independently controlled blocks, wi fi enabled and remote controlled, touch screen function for programming and running of the instrument, 3 X 32 wells block for 0.2 ml/0.1 ml. PCR tubes/PCR plates, to be supplied with all the accessories including PCR tubes for 500 reactions , USB drive and modem Flexible and efficient extraction system. Completely automatic and brand, minimum twelve samples. Suitable for wide variety of starting material, based on magnetic bead extraction technology,pre programmed along with flexible protocols, adjustable elution volume , compatible with spin column technology, automatic transfer of elutes into elution tubes with caps, UV lamp for decontamination of chamber, to be supplied with all the reagents and accessories for processing of samples for extraction of viral RNA and DNA extraction including all consumables, software, laptop, communication cables, USB stick etc. To be supplied with real time Transilluminator and 10 no. 1% and 10 No. 2% pre cast agarose gels cassettes having safe blue light excitable dye, 50 bp and 1 Kb plus DNA ladder; gel loading buffer, preprogrammed to run with gel cassettes, transilluuminator should be provided with amber color filter, Indian power cord with universal adapter for 90-220 V,50-60 Hz, can run minimum 4 samples at a time. Gr. A/ Lab. Inst./01 Pc./ 26 Box Gr. A/ Lab. Inst./01 Pc./ 27 Box Gr. A/ Lab. Inst./01 Pc./ 28 Box Gr. A/ Lab. Inst./01 Pc./ 29 Cartoon Gr. A/ Lab. Inst./01 Pc./ 30 Cartoon Gr. A/ Lab. Inst./01 Pc./ 31 Cartoon 32. Computer controlled rapid Microbial Identification system 33. Binocular microscope 34. Automatic Tissue Processor 35. UV-VIS Spectrophotometer System should be based on the technology of Advance colorimetry with a capacity to test 60 samples at a time, System should be FDA cleared with 21 CFR part 11 compliance, System should have at least 3 optical lenses to read the color change in the reactions, System should have specific cards with biochemical substrates impregnated for reaction, System should be useful for microbial identification and antibiotic susceptibility testing if required with specific antibiotic panel for veterinary use, System should be accompanied with a computer pre loaded with the system software, a densitometer, Dispensor and 2000 tubes for sample preparation, System should have an inbuilt incubator and waste disposal bin, System should have minimal manual steps and the card filling and loading should be automated, System should read the reactions in both kinetic and end point mode and give results within a day, System should be able to analyse Gram positive bacteria, Gram negative bacteria, Bacillus species, Coryne form species, yeast species and anaerobic bacteria and 200 nos. of relevant cards should be provided with the system, The software of the system should be pre loaded in the PC and should be intuitive software which is easy to use, The software should have an audit trail which would capture all the activities being done on the software, Software should have a feature to establish a new database as per the requirement of the user apart from the already existing database, Every user should be able to create and use their own login id and password for using the system, The vendor will provide installation and training on site for all the trainees in the user lab, should be provided with UPS for 30 minutes power back up, one year warranty followed by two years AMC, certificate of installation in reputed organization should be provided by the vendor. Illumination LED for transmitted light, objective turret 4 position, manual, stage fixed, to be supplied with 6 plan achromatic objectives ,4X,10X,20X,40X,100X, to be supplied with power input jack, power switch, USB port, coaxial focusing knob, Illumination wheel, freeze button, save button, with integrated software for adjustable saturation and contrast, color temperature control, to be supplied with all the accessories including 12.1’ high resolution color monitor with adjustable tilt, AC adaptor Carousel type Construction with 12 station (10 reagent station, 2 wax bath) Glass containers at each station should have capacity of 1.8 litres. Option for manual raising and rotation of carousel for immediate tissue basket removal or transfer to the next station-using crank. Automatic reheating of wax before basket transfer to a wax bath. standard tissue basket. Membrane keypad and LCD screen. .Metal tissue baskets – 1 no. s capacity approx. 80 cassettes to be run at a go. Infiltration time of up to 99 hr 59 minutes & should be separately programmable, for each station. Temperature range of wax baths 45 oC to 65 oC. Excess temperature cut out 75 oC Both immediate and delayed start option must be available. Delayed start function up to 7 days or more. Preferably 1 minute drain time between stations for reduced carry over. Safety feature for automatic immersion of tissue basket in a station in case of mains power failure. Power failure indication including station number and time lapsed in excess of programmed infiltration time. Audible alarm warning in case of error message and completion of tissue processing cycle. Android based operating system, stand alone unit, no PC is required for running the experiments, preloaded the programs for determination, concentration and purity of ss DNA, ds DNA, RNA, protein Gr. A/ Lab. Inst./01 Pc./ 32 Cartoon Gr. A/ Lab. Inst./01 Pc./ 33 Cart. Gr. A/ Lab. Inst./01 Pc./ 34 Cart. Gr. A/ Lab. Inst./01 Pc./ 35 Cart. 36. Ampoule Freeze Dryer 37. Research adjustable volume pipettes, Autoclavable with tip box Research adjustable volume pipettes, Autoclavable with tip box 38. 39. 40. 1. 2. 3. Research adjustable volume pipettes, Autoclavable with tip box PCR Plate centrifuge Polypropylene bottle Autoclavable Rubber stopper for Polypropylene bottle Aluminum cap (20 mm diam.), along with unlimited number of customs assays, can be used for microvolume and cuvette based measurements, should include a cuvette heater for providing flexibility for kinetics at temperature set point 30-45 degree C, can measure 2.0-25000 ng/µl ds DNA and 1.0 mg/ml -750 mg/ml BSA, should have full spectrum capabilities with wave length accuracy 1 nm, can measure sample as little as 0.5 µl, should include built wi-fi, Ethernet and 3 USB ports, light source should be pulsed xenon flash lamp, internal storage 8 GB flash memory, path length 10,5, 2,1 mm with accurate pathway control direction limit 0.4 ng/ µl ds DNA maximum concentration 750 ng/ µl ds DNA (Cuvette mode), operating voltage 12 VDC to be operated at 220 V, measurement time less than 4, one year warranty followed by 2 years AMC Area: 0.40 Sq.m, Volume: 44.5 litres, Ice capacity: 10.5 Kg/24hrs, Ice capacity (Max): 18.0 Kg/24hrs, condenser Ultimate low temp.: -85°C ± 5°C @ 20°C ambient, Condenser Material of Construction: SS 304, Position: Horizontal Compressor: 2 x ¾ HP, Refrigerant: CFC Free Vacuum indication: Digital pirani vacuum gauge with a range of 999 to 1 x 10-3 mbar, Vacuum pump required: 250 Lit/min, Input power supply: 220V, 50Hz, 1 Phase, Vacuum Pump 250 LPM, Oil Mist Filter, Spin Freezer temp 55°C ± 5°C with 5 interchangeable carriers with independent power supply and timer, 24-Port secondary drying manifold, Spark tester, for leak check of ampoules (Imported), Hand held ampoule sealing torch with cylinder and all the accessories for ampoule sealing Pipette ranges 0.5-10 µl, should be certified as research category, single channel Gr. A/ Lab. Inst./01 Pc./ 36 Crate Gr. A/ Lab. Inst./01 Pc./ 37 Box Pipette ranges 100-1000 µl, should be certified as research category, single channel Gr. A/ Lab. Inst./01 Pc./ 38 Box Pipette ranges 20-200 µl, should be certified as research category, single channel Gr. A/ Lab. Inst./01 Pc./ 39 Box Should be capable of centrifuging 96 wells PCR plate, fixed speed, single knob for alignment of the plate with rotor slot, capable of sort spin GROUP-B: LABORATORY REAGENTS & OTHER GLASS/ PLASTIC MATERIALS Gr. A/ Lab. Inst./01 Pc./ 40 Cartoon 110 ml capacity & 20 gm weight per bottle Gr. B/Lab. Regt./100 Pc./01 Bot. Made of rubber for 110 ml capacity & 20 gm weight polypropylene bottle Gr. B/Lab. Regt./100 Pc./02 Box Aluminum cap (20 mm diam.), for 110 ml capacity & 20 gm weight polypropylene bottle Gr. B/Lab. Regt./100 Pc./03 4. Neutral Glass vial 3 ml. capacity, 13 mm OD, made of neutral glass, height more than 30 mm Gr. B/Lab. Regt./360 pcs./04 box 5. Neoprene Rubber cap for Neutral Glass vial 3 ml. capacity, 13 mm OD Aluminium caps, coloured and non colour for Neutral Glass vial Made of neoprene rubber, double slit, double ring, suitable for above vaccine vial, specimen to be obtained from the respective units Gr. B/Lab. Regt./2000 pcs./05 Pkt. Aluminum cap for Neutral Glass vial 3 ml. capacity, caps, 13 mm. 3 ml. capacity, 13 mm OD diameter Gr. B/Lab. Regt./5000pcs./06 Pkt. 7. Gel pack(Ice cold pack) 100gm Gr. B/Lab. Regt./10 pc./07 Pkt. 8. Syringe Filter (Membrane filter) Gel pack(Ice cold pack) Tissue Culture (TC)Flask with filter cap Pore size-0.45 µm, sterile, individually packed- 50 pcs/pack Gr. B/Lab. Regt./50 pc./08 Pkt. 450 gm 300 cm2, Growth area with 0.2 µm porosity filter cap, sterilized by gamma ray irradiation, legible volume scale with white print and marking area,1360 ml. Gr. B/Lab. Regt./10 pc./09 Gr. B/Lab. Regt./18 pc./10 Pkt. Pkt 11. Tissue Culture (TC)Flask 300 cm2 Growth area, sterilized by gamma ray irradiation, legible volume scale with white print and marking area, 1360 ml. Gr. B/Lab. Regt./18 pc./11 Pkt. 12. 500 gm Gr. B/Lab. Regt./01 pc./12 Jar 500 gm Gr. B/Lab. Regt./01 pc./13 Jar AR Grade -500 ml. Gr. B/Lab. Regt./01 pc./14 Bot 25 lt. Jar As adjuvant for bacterial vaccine-400 ml. Gr. B/Lab. Regt./01 pc./15 Gr. B/Lab. Regt./01 pc./16 Jar Bot 17. 18. Thioglycollate Broth(BQ vaccine Media) Standard Nutrient Broth (HS Vaccine Media) Formaldehyde 37% (formalin) Glycerol-98% Purified Aluminum Hydroxide Gel (3 % W/V, Liquid type) pH-7 Yeast Extract Powder EMEM 500 gm 1 lit., modified for autoclaving without sodium bicarbonate and L-Glutamine, contain 9.4 gm powder/ liter of media, water by Karl Fisher NMT 2.0% , glucose 9.54-11.66%, endotoxin assay NMT 1.0EU/ML at IX Gr. B/Lab. Regt./01 pc./17 Gr. B/Lab. Regt./10 bot./18 Bot. Pack 19. L-Glutamine 0.292gm. /vial, sterile by gamma irradiation, to be used with EMEM Gr. B/Lab. Regt./10 vial/19 Pack 6. 9. 10. 13. 14. 15. 16. GROUP:C-LABORATORY KITS 1. Classical Swine Fever Antigen ELISA test kits 2. M. bovis Antibody ELISA test kit 3. Bacillus anthracis (common)PCR kit 4. 5. RT PCR kit PRRS Antibody ELISA test kit Bovine Herpes Virus (BHV-1) real time PCR kit 6. 7. 8. 9. 10. Bovine Viral Diarrhoea Virus (BVD) real time RT PCR Kit Quantitative(q) PCR mastermix kit Quantitative(q)RT PCR master mix kit DNA purification kit for whole blood and tissue 11. Viral RNA purification kit 12. Rapid AIV Detection Kit Antigen Simple, familiar ELISA format, 96-well plates, can be fully automated for high sample throughput, Short (less than 4-hour) or overnight protocol, to be used with tissue sample, cell culture fluid etc, ,to be inclusive of ready-to-use reagents and bar-coded plates for safe sample identification and traceability, to be suitable for CSFV disease detection, 5 plates/strips/ Kit No cross-reactivity in animals infected with other mycobacterium sp., no desensitization in anergic cattle, not required 60-day waiting period between tests, should give fast objective result with good traceability. Can be used with standard serum and plasma samples collected for other purposes, to be supplied/matched with a compatible software for data analysis and result interpretation, 2 hours protocol, 5 plates/strips/ Kit Ready to use Kit for conventional PCR(agarose gel based), should contain –ve control, +ve control, forward and reverse primers, Taq polymerase and all other materials except agarose and TAE buffer, 100 reactions/kit One step, For 25 reactions per pack, should support multiplex reactions. Porcine reproductive & respiratory syndrome viral antibody ELISA test kit, 480 tests/kit, single well format Contains primers and probes(FAM labeled BHQ quenched) with 100 % homology with reference sequence contained in the NCBI database, along with defined number of positive template control with defined copy number for construction of standard curve, internal extraction control DNA, internal extraction control primer/probe mix. Contains primer and probe (FAM labeled BHQ quenched) against BVD virus polyprotein gene along with template control with defined copy number for construction of standard curve to quantify the virus, internal extraction control RNA, internal extraction control primer/probe mix . Gr. C/Lab. Kit./1kit/1 Box Gr. C/Lab. Kit./5kit/2 Box Gr. C/Lab. Kit./1kit/3 Box Gr. C/Lab. Kit./1kit/4 Gr. C/Lab. Kit./1 kit/5 Box Box Gr. C/Lab. Kit./150 tests/ 6 Box Gr. C/Lab. Kit./150 tests/ 7 Box For multiplex real time PCR reaction, for 200 reactions of reaction volume 50 µl, to be supplied with 3X1.7 ml. multiplex PCR master mix, 2 X 2 ml. nuclease free water For multiplex real time RT PCR reaction, for 200 reactions of reaction volume 50 µl, to be supplied with multiplex RT PCR mastermix 3 X 1.7 ml.,multiplex RT mix, Rnase free water 2 X 2 ml. Capable of purification of DNA from animal whole blood, animal tissue, plasma, cell free fluids, cultured cells, mouse tail, to be supplied with spin columns, tissue lysis buffer,elution buffer, washing buffer, collection tubes, Proteinase K solution, RNAse A solution, resuspension solution,250 purification/kit Gr. C/Lab. Kit./ 100 Reaction/Kits/ 8 Gr. C/Lab. Kit./ 100 Reaction/Kits/ 9 Gr. C/Lab. Kit./ 250 purification / 1 kit /10 Box For viral RNA purification from plasma, serum and cell free body fluids, to be supplied with mini spin columns, carrier RNA, buffers and collection tubes, 50 purification/kit 30 tests/pack Gr. C/Lab. Kit./ 50 purification /1kits/11 Gr. C/Lab. Kit /30 tests/pack/12 Box Box Box Box 13. 14. 15. 16. 17. 18. IBD Virus Antigen Test Kit NDV ELISA Kit (Ab. Detection) Rapid IBD test kit Rapid NDV Antigen Test kit Sandwitch ELISA test Kit for BSE Purification of PrPsc. Avian Influenza Virus subtype (H5) Real time kits 19. Avian Influenza Virus subtype (H7) Real time kits 20. Avian Influenza Virus subtype (H9) Real time kits 21. AIV Antibody ELISA Kit 22. Rapid AIV subtype H5Antigen detection kit Avian Influenza Virus subtype (H5) Antibody detection kit Avian Influenza Virus subtype (H7) Antibody detection kit Newcastle Disease (ND) Virus Real time kits 23. 24. 25. Gr. C/Lab. Kit /10 tests/kit/13 Box Gr. C/Lab. Kit /480 tests/pack/14 Box Gr. C/Lab. Kit /10 tests/pack/15 Box Gr. C/Lab. Kit /10 tests/pack/16 Box 192 tests/kit i) TeSeETM Purification Kit - 192 test sii) Calibration Syringe & Needle (X100) iii) Calibration Syringe & Needle (X100) Detection Kit: TeSeE Detection kit (192 tests) Bovine Sampling Syringe. For Haemaglutinin H5 gene, H5 specific primer/probe mix (150 reactions)FAM labeled BHQ quenched, H5 positive control template, Internal extraction control RNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, For Haemaglutinin H7 gene, H7 specific primer/probe mix (150 reactions)FAM labeled BHQ quenched, H7 positive control template, Internal extraction control RNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, For Haemaglutinin H9 gene, H9 specific primer/probe mix (150 reactions)FAM labeled BHQ quenched, H9 positive control template, Internal extraction control RNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, Competitive ELISA Kit for qualitative detection of antibody to the most common and prevalent AIV in chicken , duck turkey, quail, goose, guinea partridge, red partridge, pheasant, swan, horse/swine samples 480 Test /Kit 25 test/kit Gr. C/Lab. Kit /192 tests/Kit/17 Box Gr. C/Lab. Kit /150 tests/pack/18 Box Gr. C/Lab. Kit /150 tests/pack/19 Box Gr. C/Lab. Kit / 150 test /Kit/20 Box Gr. C/Lab. Kit / 480 test /Kit/21 Box Gr. C/Lab. Kit / 25 test /Kit/22 Box 192 test /Kit Gr. C/Lab. Kit / 192 test /Kit /23 Box 192 test /Kit Gr. C/Lab. Kit t/ 192 test /Kit/24 Box For M gene, NDV specific primer/probe mix (150 reactions)FAM labeled BHQ quenched, NDV positive control template, Internal extraction control RNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, For rapid and validated diagnosis of the subtype, 150 Gr. C/Lab. Kit /150 tests/pack/25 Box 10 tests/kit 480 tests/kit 10 tests/pack 10 tests/pack 26. Avian Bronchitis Time Kits 27. Avian Adenovirus(Egg Drop Syndrome) 28. Mycoplasma gallisepticum Real Time Kits 29. Brucella genus Real Time kits 30. Camylobacter foetus Real Time Kits 31. Salmonella detection PCR kits E coli 0157:H7 detection PCR kit SYBR Green I PCR kits 32. 33. 1. Infectious Virus, Real 2. Basic synthesis(Per base charges) for Primers Probe 3. Probe tests/pack For IBV specific primers and probes (150 reactions)FAM labeled BHQ quenched, IBV positive control template, Internal extraction control RNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, For EDS specific primers and probes (150 reactions)FAM labeled BHQ quenched, EDS positive control template, Internal extraction control DNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, For M.gallisepticum specific primers and probes (150 reactions)FAM labeled BHQ quenched, EDS positive control template, Internal extraction control DNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, Brucella spp. specific primer/probe mix (150 reactions)FAM labeled BHQ quenched, Brucella spp. positive control template, Internal extraction control DNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, C. foetus specific primer/probe mix (150 reactions) FAM labeled BHQ quenched, C. foetus. positive control template, Internal extraction control DNA, Internal extraction control primer/probe mix (150 reactions) VIC labeled, Endogenous ACTB primer/probe mix (150 reactions) FAM labeled, BHQ quenched, RNAse/DNAse free water, Semi quantitative25 runs/kit PCR kit & primer mix-1 box25 reactions/kit 2x SYBR Green PCR Master Mix, Hot Start Taq DNA Polymerase, SYBR Green PCR Buffer, dNTP mix, including dUTP, SYBR Green I, 5 mM MgCl2 , 200 runs X 50µl, For Real time assay using SYBR green I dye, 200 runs X 50µl, Gr. D-LABORATORY MOLECULAR REAGENTS HPLC purified, salt free oligos, length restriction (mer) 5-49, synthesis scale tube 0.03µmole (30nm), to be supplied positively within 15 working days, per base charges to be included. Reporter dye – FAM/VIC/HEX/Cy5; Quencher dye – BBQ; Condition: TMA 1.0 OD, HPLC purified. Lyophilized. Rate should be quoted per base pair. Charges for any LNA have to be quoted separately. Amount and concentration of the reagent has to be quoted. Reporter dye – FAM/VIC/HEX/Cy5; Quencher dye – BHQ 1; Condition: TMA 1.0 OD, HPLC purified. Lyophilized. Rate should be quoted per base pair. Charges for any LNA have to be quoted separately. Amount and concentration of the reagent has to be quoted. Gr. C/Lab. Kit /150 tests/pack/26 Box Gr. C/Lab. Kit /150 tests/pack/27 Box Gr. C/Lab. Kit /150 tests/pack/28 Box Gr. C/Lab. Kit /150 tests/pack /29 Box Gr. C/Lab. Kit /150 tests/pack/30 Box Gr. C/Lab. Kit /25 runs/kit/31 Box Gr. C/Lab. Kit /25reactions/kit/32 Box Box Gr. C/Lab. Kit /200 runs/33 Gr. D/Lab. Regt./1 vial/1 Cartoon Gr. D/Lab. Regt./1vial/2 Cartoon Gr. D/Lab. Regt./1 vial /3 Cartoon 4. Primer, forward 5. Primer, Reverse Synthesis scale tube 0.03 micro molar, length restriction 5- 40 (Mer), minimum yield OD 3.0, rate to be quoted price per base, High prity salt free Synthesis scale tube 0.03 micro molar, length restriction 5- 40 (Mer), minimum yield OD 3.0, rate to be quoted price per base, High prity salt free Gr. D/Lab. Regt./ 1 vial /4 Cartoon Gr. D/Lab. Regt./1 vial/5 Cartoon Sd/(Dr. S. Bose) Director of Animal Husbandry & Veterinary Services, West Bengal