IGCSE BIOLOGY Cellular organization State that living organisms are made of cells All animals, plants, fungi, protists, & bacteria are made of one or more cells. Cells are the smallest unit of life. Most human cells, like most other animal cells, have the following parts: nucleus cytoplasm cell membrane mitochondria ribosomes Plant cells also have: cell wall chloroplasts permanent vacuole 1 Identify and describe the structure of a plant cell (palisade cell) and an animal cell (liver cell), as seen under a light microscope. palisade cell liver cell Photos produced by microscopes are called micrographs. 2 Light micrographs Transverse section of upper part of leaf of a Helleborus sp. showing epidermis and palisade cells. Light micrograph of a transverse section through a holly leaf, Ilex sp. . Below the epidermis (top layer of cells), are two layers of column-like cells known as palisade cells. These contain large numbers of chloroplasts and are the principal sites of photosynthesis. Magnification: x240 at 35mm size. Tea leaf. Light micrograph of a cross-section through a tea (Camellia sinensis) leaf. The upper and lower epidermis on the surfaces of the leaf are blue. Under the upper epidermis are palisade cells (brown), which contain chloroplasts, the site of photosynthesis. Beneath this a spongy mesophyll layer with large spaces between the cells. At bottom left, a stoma (pore) is seen. Stomata allow gases and water to enter and leave the plant. Magnification: x230 when printed 10 centimeters wide. 3 Color enhanced freezefracture Scanning Electron Micrograph (SEM) of leaf of Lupin plant (Lupinus), showing vascular bundle, epidermis, palisade cells and spongy mesophyll. Magnification x540 Scanning Electron Micrographs Scanning electron micrograph of cross fracture of the leaf Zinnia elegans, showing the upper epidermis and palisade cells within which are stored the chloroplast (photosynthesis). X 2100. Leaf section. Colored scanning electron micrograph (SEM) of a section through a fractured leaf. At right is a single layer of cells that forms the epidermis of the leaf. The top layer (seen here at left) of the leaf interior is made up of palisade mesophyll tissue. One of the tightly packed palisade cells (light green) has been fractured open to reveal its chloroplasts (orange/green), the sites of photosynthesis. 4 Describe the differences in structure between typical animal and plant cells. Generalized animal and plant cell *Make sure you can label diagrams of animal and plant cells. 5 Describe the differences in structure between typical animal and plant cells. PLANT CELLS Have a rigid cell wall that helps them keep their shape. Plants manufacture their own food and use chloroplasts to do that. Usually have a large central vacuole. ANIMAL CELLS Do not have a cell wall and therefore require either external or internal support from some kind of skeleton. Have a shape related to their function. Animals must eat their food because they cannot photosynthesize. Animal cells may have several small vacuoles, or none at all. 6 Relate the structures seen under the light microscope in the plant cell and in the animal cell to their functions. Animal cells and plant cells have some organelles in common. organelle function nucleus contains genetic material, which controls the activities of the cell cytoplasm most chemical processes take place here, controlled by enzymes cell membrane controls the movement of substances into and out of the cell mitochondria most energy is released by respiration here ribosomes protein synthesis happens here Plant cells also have a cell wall, and often have chloroplasts and a permanent vacuole. organelle function cell wall strengthens the cell chloroplasts contain chlorophyll, which absorbs light energy for photosynthesis permanent vacuole filled with cell sap to help keep the cell turgid 7 Relate the structure of the following to their functions • red blood cells – transport • root hair cells – absorption Specialized cells: Cells may be specialized for a particular function. Their structure allows them to carry out this function . Examples of specialized cells Cell Function Adaption Absorbs light energy for photosynthesis Packed with chloroplasts. Regular shaped, closely packed cells form a continuous layer for efficient absorption of sunlight. Absorbs water and mineral ions from the soil Long 'finger-like' process with very thin wall, which gives a large surface area. Leaf cell Root hair cell 8 Cell Function Adaption Fertilizes an egg cell female gamete The head contains genetic information and an enzyme to help penetrate the egg cell membrane. The middle section is packed with mitochondria for energy. The tail moves the sperm to the egg. Contain hemoglobin to carry oxygen to the cells. Thin outer membrane to let oxygen diffuse through easily. Shape increases the surface area to allow more oxygen to be absorbed efficiently. No nucleus, so the whole cell is full of hemoglobin. Sperm cell Red blood cells Nerve cells To carry nerve impulses to different parts of the body Long To join with male cell, and then to provide food for the new cell that's been formed Large Female reproductive cell (egg cell) Connections at each end Can carry electrical signals Contains lots of cytoplasm 9 Calculate magnification and size of biological specimens using millimeters as units. Practice: 1. using the images of the cells on pages 3 and 4 of these notes, calculate the actual size size of some of the structures seen. 2. Search the internet for light and electron microscope images of various cells. If they have a scale bar, print them and calculate the real size of the sample. 10 Know that cells are arranged in groups to form tissues. tissue – a structure made from large numbers of one type of cell Examples of tissues found in the human body 11 Examples of tissues found in a leaf 12