HL IB-Biology 2011/12 #18 PLANT STRUCTURE 9.1 Plant Structure and Growth Assessment Statements Addressed: 9.1.1 Draw and label plan diagrams to show the distribution of tissues in the stem and leaf of a dicotyledonous plant. 9.1.2 Outline three differences between the structures of dicotyledonous and monocotyledonous plants. 9.1.3 Explain the relationship between the distribution of tissues in the leaf and the functions of these tissues. 9.1.4 Identify modifications of roots, stems and leaves for different functions: bulb, stem tubers, storage roots and tendrils. Part I: DISTRIBUTION OF TISSUES IN THE STEM AND LEAF OF DICOTYLEDONOUS PLANTS Text adapted from: Damon et al, Higher Level Biology, Heinemann Baccalaureate 2007 Land plants possess some common characteristics: • They provide protection for their embryos which has increased over time • They have multicellular haploid and diploid phases • They can be compared by the presence or absence of conductive systems Table 1: LAND PANTS ARE DIVIDED INTO THREE MAJOR GROUPS: MAJOR FEATURES PLANT GROUP NON-VASCULAR LAND PLANTS SEEDLESS VASCULAR PLANTS SEED VASCULAR PLANTS Co conductive tissue Often grouped together as bryophytes Usually small and grow close to the ground Include mosses, liverworts., and hornworts Well developed vascular tissue Do not produce seeds Include horsetail, ferns, club mosses, and whisk ferns Most living plant species are in this group Seeds contain an embryo, a supply of nutrients, and a protective outer coat Have extensive vascular tissue and include some of the world’s largest organisms The seeded vascular plants are further divided into: • Gymnosperms - have seeds that do not develop within an enclosed structure • Angiosperms - have seeds that develop within a protective structure Labudda, CHS, 18 Plant Structure.pages We are looking at the angiosperms. In general, angiosperms have thee basic types of tissue: 1. Dermal tissue - this outer protective covering protects against physical agents and pathogenic organisms; it prevents water loss and may have specialized structures for various purposes; 2. Ground tissue - consists mostly of thin - walled cells that function in storage, photosynthesis, support and secretion; 3. Vascular tissue - xylem and phloem carry out long distance conduction of water, minerals and nutrients within the plant and provide support. These three tissue types derive from meristematic tissue. Meristematic tissue is composed of aggregates of small cells that have the same function as stem cells in animals. When these cells divide, one cell remains meristematic while the other is free to differentiate and become part of the plant body. By doing this, the population of meristematic cells in continually renewed. The cells that remain meristematic are referred to as initials, while the cells that begin differentiation are called derivatives. Plants have organs just as complex as animals do. The three major organs are roots, stems, and leaves. Root tissues The rootʼs function is to absorb mineral ions and water from the soil. Roots anchor the plant and even provide food storage in some cases. They have an epidermis that forms an protective outer layer. Following the epidermis is the cortex which is involved in conducting water from the soil to the interior vascular tissue of the root. The cortex may also be modified to carry out storage function. Next time you eat a whole (real) carrot (not a baby carrot), break it in half, and identify the cortex and vascular tissue. In the middle of the root is the vascular tissue that contains the phloem, which transports organic nutrients and xylem, which transports water and dissolved minerals. The xylem is located at the innermost part of the root, surrounded by the phloem. The vascular tissue is surrounded by an endodermis. Stem tissues The stem is the plant region where leaves are attached. The area where a leaf joins the stem is called the node, and the area between two nodes is called an internode. Leaves are arranged in a number of different ways of the stem. The arrangement of tissues in the stem is as follows: The stem is surrounded by an epidermis which mainly serves as a protection, however, it can have pores (lenticles) that allow gas exchange. Followed by the epidermis is the cortex. The cortex of the stem resembles that of the root. It supports and may have storage function. The transporting tissues of the stem include xylem and phloem. They are arranged together as vascular bundles in a circle with the xylem towards the inside and the phloem towards the outside of the stem. The xylem transports water and dissolved minerals. In woody plants it also lends support to the plant. The phloem mainly transports organic nutrients throughout the plant. The tissue that separates the xylem from the phloem in the stem is called the cambium. The cambium is an area of rapidly dividing cells that differentiate into xylem and phloem. In the very middle of a stem cross section is the pith region which functions as storage and support. Turgid, fluid filled cells in the cortex and pith offer support to the plant. Leaf tissues Leaves are involved in photosynthesis. They vary greatly in form but they generally consist of a flattened portion called the blade and a stalk called petiole that attaches the blade to the stem. The tissues are distributed as follows: Many leaves have a layer of wax called the cuticle as their outermost layer. This layer protects against water loss and insect invasion. If a cuticle is not present, the outermost layer is the epidermis which protects. In leaves with cuticle the epidermis follows the cuticle and is called the upper epidermis. Like stems and roots, the leaves have vascular tissue which includes xylem and phloem. The xylem brings water to the leaves while the phloem carries the products of photosynthesis to the rest of the plant. The xylem and phloem occur together in veins or vascular bundles. A densely packed region of cylindrical cells occurs in the upper portion of the leaf. This region is called the palisade mesophyll. The cells here contain large numbers of chloroplasts to carry out photosynthesis. The bottom portion of the leaf is composed of the spongy mesophyll. It consists of loosely packed cells with few chloroplasts. There are many air spaces in this area providing gas exchange surfaces. Stomata occur on the bottom surface of leaves and they allow oxygen and carbon dioxide exchange. Specialized cells called guard cells control the opening and closing of stomata. ‣ Find and study drawings showing the distribution of tissues in the stem and leaf of a dicotyledonous plant. Review the function of each tissue in the stem and the leaf. Note how the relative location and distribution of the tissues support their function. NOTES: Part II: MICROSCOPY LAB Handling the Microscope: • Carry using both hands; one at arm the other supporting the base • Place on table gently • Do not touch lenses • Return on low power • Make sure slide is removed when returning Focusing: • Always start on low power, using COARSE focus ONLY! • Switch to medium power without moving the stage • Refocus using the coarse adjustment (only minor adjustments are needed) • Switch to high power without moving the stage • Only use FINE focus under high power! Measuring with the Microscope: We will estimate the size of a microscopic structure by comparing it to the known sizes of the diameter of the field of view and the length and width of the pointer. Refer to the chart in class for the measurements. Making to Scale Drawings of Microscopic Structures: ‣ Every drawing needs to provide enough information about the actual size of the structure shown. This can be done several ways: • Indicate in your drawing the part you measured and write the actual measurement next to it. • You calculate how much bigger your drawing is than the actual size and write the magnification factor next to your drawing. • Provide a scale bar at bottom of the drawing ‣ Title each drawing with the structure observed and power used ‣ Each drawing should take up ½ page, do not draw the field of view ‣ All structures shown should be labeled. Draw a line from the structure to the OUTSIDE of your drawing and add the label ‣ All drawings should be neat! TASK: You have available prepared slides of cross-sections of stem and leaf of a dicotyledonous plant. 1. Look at each cross-sections and make detailed (show cellular detail) and labeled drawings under low, medium, and high power. You might have to make several high power drawings to show different structures in more detail. Use the information from part I to identify all the tissues found in each organ. 2. Once you are done with your detailed drawings, make a plan diagram of a cross section of a stem and leaf to show the position of the different tissues. Remember a plan diagram does not show the individual cells just the relative position of the different tissues. 3. Add a description of the function next to the label for each tissue shown in the plan diagram 4. Copy table 2 under your plan diagrams and complete the table by explaining for each tissue how how location supports function. Table 2: How location of leaf tissue supports its function Leaf tissue Palisade mesophyll Veins Spongy mesophyll Stomatal pores How location supports function: Part III: MONOCOTS, DICOTS AND PLANT ORGAN MODIFICATIONS Monocots Versus Dicots Angiospermophytes are organized into monocotyledonous and dicotyledonous plants, based on their morphological similarities. Morphological similarities between different groups of organism can imply, that the groups are closely related to each other. However, some structural similarities can be a result of adaptations to the same selection pressure (bat wing and bird wing). Evolutionary relatedness can be shown in form of a cladogram. A branching point represents the last common ancestor of two groups of organisms. Any characteristics the two groups have, that are due to evolutionary relatedness, the common ancestor should have as well. If a group has a characteristic the common ancestor or the other group does not share, then it means that characteristic evolved after the split. 1. Using the internet, research how much the morphological differences between monocots and dicots represent an evolutionary relationship. In other words, which cladogram more closely resembles current DNA evidence for “degree of relatedness”? Circle the ‘correct’ cladogram in figure 1 and provide a justification for your choice below. Figure 1: Possible Cladograms for the evolutionary Relationship Between Monocots and Dicots. dicot Common ancestor monocots Common ancestor dicots Common ancestor angiosperm Justification: monoc monoc dicot dicot Common ancestor dicot 2. Table 2 compares the the structures common to all monocots and dicots. Describe how each structure looks like for monocots and dicots and provide a drawing to clarify your description. Table 2: MAIN STRUCTURAL DIFFERENCES BETWEEN MONOCOTS AND DICOTS MONOCOTS STRUCTURE DESCRIPTION VEINS IN LEAVES FLOWER PARTS NUMBER OF COTYLEDONS (SEED LEAVES) VASCULAR BUNDLES IN STEM ROOT SYSTEM POLLEN GRAIN DICOTS DRAWING DESCRIPTION DRAWING Plant Organ Modifications Roots, stems, and leaves in plants can be modified to serve a different function, such as food storage or climbing. 1. Obtain and cut crosswise a whole carrot (not a baby carrot) and a potato. Based on what you have learned identify the tissues that are visible and decide if a carrot or potato are a modification of a root, stem, or leaf. 2. Draw and label the cross section of the carrot and potato. 3. Below the drawing state which modification the carrot and potato represent and provide a justification. (FYI: Repeat with a baby carrot. Was the baby carrot a part of a bigger carrot or is it a whole carrot but smaller?) Drawings: Justification: 4. Tables three to five list the different kinds of modifications for roots, stems and leaves. Describe each modification and provide an example. Table 3: MODIFICATION OF ROOTS ROOT MODIFICATION DESCRIPTION EXAMPLE DESCRIPTION EXAMPLE PROP ROOTS STORAGE ROOTS PNEUMATOPHORES (AIR ROOTS) BUTTRESS ROOTS Table 4: MODIFICATION OF STEMS STEM MODIFICATION BULB TUBERS RHIZOMES STOLONS Table 5: MODIFICATION OF LEAVES LEAF MODIFICATION TENDRILS REPRODUCTIVE LEAVES BRACTS OR FLORAL LEAVES SPINES DESCRIPTION EXAMPLE